complications postoperative care and management of adverse events

... The lack of hydroxylation of vitamin D3 at position 22 now provides a clear explanation for the inability of P450scc to cleave the side chain of vitamin D3 Hydroxylation of the side chain of cholesterol ... C22 and C23 of these molecules The biological significance of P450scc-catalysed metabolism of vitamin D and its precursors cannot be underestimated because P450scc-generated hydroxy derivatives of ... identification of new metabolites of vitamin D3 made by the action of P450scc will enable us to proceed with further biological testing of 2594 these compounds and the investigation of whether this...

Ngày tải lên: 18/02/2014, 17:20

... was expression of CYP4x1 RNA in brain, heart and kidney, and lesser expression in skeletal muscle and liver, and no detectable expression in other tissues However, probing of a blot of heart tissues ... (07 ⁄ 05), and of these, 18 were from brain, three were from aorta and 21 were from tumours or cell lines, confirming the importance of brain and aorta as sites of expression Cloning of mouse Cyp4x1 ... weight of 58 556 Da, a pI of 7.19, with 94 and 71% identity with the rat and human CYP4x1 proteins, respectively (Fig 1B) A genomic fragment of the CYP4x1 was cloned, containing 247 bp of intron and...

Ngày tải lên: 19/02/2014, 07:20

... the control, namely dicofol production by CYP6A2vS and DDA, and DDD and dicofol production by CYP6A2vSVL ND, not detected; NC, not calculated DDT metabolite DDA DDD Dicofol Membrane preparation ... affinity of DDT and testosterone for CYP6A2wt and CYP6A2vSVL For each apparent affinity calculated, the mean ± SD and the number of experiments (in parentheses) are given Statistical analysis of the ... and these locations (junction between helices B¢ and C, junction between b sheets 4–1 and 4–2) are very different from the locations of the mutated positions in CYP6A2 (helix J and vicinity of...

Ngày tải lên: 19/02/2014, 12:20

... lifetimes of carbon-centred radicals derived from a diverse set of substrates are on the order of about 10)10 s [98] Moreover, the randomness of the apparent intramolecular KIEs for unrearranged and ... result in a drastic diminution of polymerization products derived from aniline and some of its p- and N-substituted congeners, while formation of p-hydroxylated and dealkylated metabolites was ... discounted in view of the high selectivity of DNA strand breakage [239] Also, density functional theory (DFT) calculations of the electronic structure of an optimized geometric model of activated BLM...

Ngày tải lên: 19/02/2014, 16:20

... presence (B) of NADPH Incubations were performed at 27 °C and contained 20 pmol of CYP77A4 They were stopped after 30 by the addition of 20 lL of acetonitrile (containing 0.2% acetic acid) and directly ... presence (B) of NADPH Incubations were performed at 27 °C and contained 20 pmol of CYP77A4 They were stopped after 30 by the addition of 20 lL of acetonitrile (containing 0.2% acetic acid) and directly ... presence (B) of NADPH Incubations were performed at 27 °C and contained 20 pmol of CYP77A4 They were stopped after 30 by the addition of 20 lL of acetonitrile (containing 0.2% acetic acid) and directly...

Ngày tải lên: 07/03/2014, 03:20

... reductive and oxidative half-reactions of the enzyme, resulting in a multitude of rst- and second-order steps contributing to the observed kcat and Km values To assist in the deconvolution of possible ... re-analysed using global analysis The assignment of E1 and E2 to the FMN and of E3 and E4 to the FAD cofactor, respectively, corresponds to the analysis of Munro et al [19] FMN E1 pH 7.5 7.5 (+1 mM NADP+) ... (B) Figure S5 presents the ratio of kfast and kslow in H2O and D2O as a function of the pH value (D) The pH dependence of the SKIEs for the up phase (up-triangles) and down phase (down-triangles)...

Ngày tải lên: 07/03/2014, 06:20

... formation of P450nor with NADH Materials and methods Mutagenesis and expression plasmids The construction of each mutant of P450nor of F oxysporum was carried out according to a standard method ... other mutations (D88A and D88V) On the other hand, the accumulation of I was still observed even in the case of slow reduction of the D88N mutant with a higher concentration of NADPH (1.0 mM; Table ... was performed by means of PCR [14] using template pfp(450)-20 [15], which consists of the P450nor cDNA of F oxysporum and the pUC18 vector The primers used were M13-47 and M13RV (Takara, Otsu,...

Ngày tải lên: 07/03/2014, 15:20

... characterization of one of the minor hydroxylation products of testosterone, with an estimated total amount of  lg Spectroscopy alone yielded an unequivocal assignment of the product Traces of a product ... is of interest in considerations of the steroselectivity of P450 3A4 and general considerations about the juxtaposition of the substrate in the active site, particularly in predicting sites and ... and characterization of enzymes and nucleic acids In Principles and Methods of Toxicology (Hayes, A.W., ed.), pp 1625–1687 Taylor & Francis, Philadelphia 25 Guengerich, F.P (1988) Oxidation of...

Ngày tải lên: 07/03/2014, 16:20

... determination of the protoheme content of NHis-P450mor as pyridine hemochromogen B Sielaff and J R Andreesen firmed by spectral analysis of NHis-P450mor First and second deviations of spectra were ... the stimulation of the activity of FdRmor towards cytochrome c by wt-Fdmor, indicating an apparent Vmax of 1534 ± 29 electronsÆmin)1 and an apparent Km of FdRmor for wt-Fdmor of 316 ± 17 nm Using ... led to some loss of cofactor The highest ratio was found for NHis-Fdmor, which might indicate less efficient incorporation of the Fe3S4 cluster and ⁄ or lower stability of the cofactor, compared...

Ngày tải lên: 07/03/2014, 16:20

... incubation of D3 with P450scc, 0.26 lg of TLC-purified P1 product was obtained, representing a yield of 16% from the original vitamin D3 The yield of TLC-purified products P2 and P3 were 1.7 and 4%, ... time and mass spectrum matching the corresponding standard None of the major peaks showed detectable amounts of species with m ⁄ z at 417, 399 and 381 that represent dihydroxyvitamin D3 and products ... comparison with Fig and consisted of 85% methanol and 0.1% acetic acid from to 25 min, followed by linear gradient to 100% methanol and 0.1% acetic acid from 25 to 35 min; and 100% methanol and 0.1% acetic...

Ngày tải lên: 07/03/2014, 21:20

... Universities and complied with the ÔPrinciples of Laboratory Care and the ÔNIH Guide for the Care and Use of Laboratory AnimalsÕ 1996 Adrenal glands, allocated for RNA isolation, were flash frozen and ... dual activity of CYP17 and the differential regulation thereof have been the subject of many studies It is apparent that the normal expression and dual hydroxylase and lyase activities of this key ... activity for PREG and PROG away from the competitive influence of 3b-HSD and CYP21 Metabolism of PREG and PROG by human CYP17 was also determined to allow comparison of human and baboon CYP17 in...

Ngày tải lên: 08/03/2014, 09:20

... its keto group and the hydroxyl group of the amino-acid residue Tyr-96, and (b) by hydrophobic contacts of its methyl groups C-8, C-9 to Val295 and Asp297 in the b3 sheet, and of the methyl group ... 2002 2990 C Jung et al (Eur J Biochem 269) Fig Structure of the active site of cytochrome P450cam and of camphor analogues Top, heme and amino acids contacting the substrate 1R-camphor, PDB accession ... volume and energetic properties of the binding of CO to hemeproteins Biophys J 66, 89–98 12 Taube, D.J., Projahn, H.-D., van Eldik, R., Magde, D & Traylor, T.G (1990) Mechansim of ligand binding...

Ngày tải lên: 08/03/2014, 23:20

... mixture of d0 and [methyl-d3] 7-OMe coumarin was used as the substrate, and the kinetic isotope effect was measured from the ratio of d2 ⁄ d0 formaldehyde product b A : molar mixture of d0 and [1-ethyl-d2] ... assays with d0 and perdeuterated (at the carbon being oxidized) substrates and comparing the v versus S plots (Figs and 3, and Tables and 3) The patterns were very similar for 7-OMe and 7-OEt coumarin, ... (2001) Analysis and characterization of enzymes and nucleic acids In Principles and Methods of Toxicology (Hayes, AW, ed.), pp 1625–1687 Taylor & Francis, Philadelphia, PA, USA 35 Sandhu P, Guo...

Ngày tải lên: 16/03/2014, 13:20

... 20E up to 80% of transformation (Fig 2), which is independent of the amount of E applied (1.9 pmol, 8.6 nmol and 86 nmol EÆ100 mg calli)1) After E treatment the bioconversion rate of E to 20E (Fig ... v) glycerol to a final protein concentration of 1–2 mgÆmL)1 and stored at )80 °C until use Microsomes from 100 g of P americana mesocarp and from 17 g of rat liver were obtained using the same protocol ... to 50 lL and screened for the presence of P450 The amount of protein was determined using the Bradford procedure with bovine serum albumin as a standard [42] The protein concentration of partially...

Ngày tải lên: 16/03/2014, 23:20

... and 21 h after treatment (Fig 5A) We found the accumulation of both isoforms of C/EBPa (p42, of 42 kDa and p30, of 30 kDa) to occur concomitantly with the increase in the relative abundance of ... observations of the induction of C/EBPa mRNA and protein preceding the accumulation of CYP3A1 mRNA in rat liver suggest the importance of the characterized C/EBPa promoter elements in the up-regulation of ... 0.5 lg of 3A1-C/EBP-RE-T81Luc and 0.15 lg of pMSV/EBPa expression vector, or empty vector The normalized luciferase activities are expressed as mean values ± SD of duplicates for a minimum of three...

Ngày tải lên: 17/03/2014, 09:20

... CYP2C5 is present and the C-terminal part of SRS is a b-strand not involved in a b-sheet with SRS The alignment of SRS of CYP2C5 and the whole CYP2B family with those of CYP73A1 and CYP71Ds is not ... product Superimposition of the NA and CA structures, and of their positions of attack on those of I2C, indicates that P2 is most likely 6-hydroxy-I2C, although an authentic standard was not commercially ... binding and correct positioning of CA during catalysis A role of N302 in anchoring the side-chain carboxylate of CA is further supported by the enhanced binding of amine substituted ligands and also...

Ngày tải lên: 17/03/2014, 10:20

... retention time and MS cleavage of Ala1-TTX; and (c) the relative amounts of M1 and M2 were different depending on the acidity of the HPLC eluent or on the time elapsed between incubation and analysis ... and characterization of stable carbinol-amide L Perrin et al Table Amounts of metabolites M1 and M2 recovered after 10 incubations of 100 lM tentoxin using lM of rat microsomal preparations and ... characterization of stable carbinol-amide Table MS and MS-MS fragments of M1 TTX metabolite (in bold) and tentative assignment of loss of fragments obtained after HPLC-MS ESI in positive and negative...

Ngày tải lên: 22/03/2014, 16:20

... matrix of the cuticle Reverse genetics approaches in Arabidopsis and potato enabled investigation of the putative involvement of other members of the CYP86 family in the synthesis of cutin and the ... condensation and elongation of the polymers Ensuring reproductive success was also of primary importance for land conquest Cutin of the anther wall and sporopollenin represent protective envelopes and ... thaliana of a second plant FA hydroxylase Using the similarity of an Arabidopsis expressed sequence tag (EST) sequence and the consensus sequence of the fungal CYP52 family of alkane hydroxylases and...

Ngày tải lên: 22/03/2014, 16:21

... degradation of xenobiotics and synthesis of polyketide antibiotics Most enzymes can handle a broad range of substrates, although CYP105D5 activity is restricted to fatty acids only x-1, x-2, x-3 and ... Eshoo M & Wilson CR (2003) Identification and characterization of the CYP52 family of Candida tropicalis ATCC 20336, important for the conversion of fatty acids and alkanes to alpha, omega-dicarboxylic ... proven to be pseudogenes Well-studied members of the CYP52 family are the enzymes of the alkane-metabolizing yeasts Candida tropicalis, Candida maltosa and Yarrowia lipolytica C tropicalis has at...

Ngày tải lên: 22/03/2014, 16:21