complex model suitable for high throughput oncogene screening

... Page of 13 (page number not for citation purposes) Retrovirology 2004, Conclusions This new CD4+/CCR5+/CXCR4+ cell line described here is most valuable as a tool for high- throughput evaluation of ... it can be considered as a highly specific CXCR4 antagonist [26-28] It was demonstrated that two aspartate residues at positions 171 and 262 of CXCR4 are crucial for the high- affinity binding of ... CXCR4 expression, we used the U87.CD4 cell line as a starting point to create a cell line highly suitable for the evaluation of the anti-HIV activity of potential CCR5 and CXCR4 antagonists The...

Ngày tải lên: 13/08/2014, 13:20

Ngày tải lên: 21/02/2014, 05:20

... can be produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production ... proteins makes it an attractive tool for preliminary studies prior to the production of uniformly [15N ⁄ 13C]labelled samples for in-depth NMR analysis Much information can be gleaned already from ... particularly attractive for high- throughput production of proteins and selective isotope labelling starting from relatively expensive isotope labelled amino acids A straightforward combinatorial...

Ngày tải lên: 07/03/2014, 12:20

... Bioinformatics for High Throughput Sequencing Naiara Rodríguez-Ezpeleta Ana M Aransay ● Michael Hackenberg Editors Bioinformatics for High Throughput Sequencing Editors ... MG-RAST, a high- throughput system built to provide high- performance computing to researchers interested in analysing metagenomic data 1.5 What is Next? The increasing range of high- throughput ... al (eds.), Bioinformatics for High Throughput Sequencing, DOI 10.1007/978-1-4614-0782-9_2, © Springer Science+Business Media, LLC 2012 11 12 S Myllykangas et al Fig 2.1 High- throughput sequencing...

Ngày tải lên: 29/05/2014, 14:33

... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... generalized modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...

Ngày tải lên: 18/06/2014, 18:20

... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... generalized modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...

Ngày tải lên: 20/06/2014, 01:20

... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... generalized modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...

Ngày tải lên: 20/06/2014, 21:20

... developed for the identification of molecules in complex shotgun spectra datasets It formalizes the available or assumed knowledge of lipid fragmentation pathways into queries that are used for probing ... spectrometer LipidXplorer imports spectra in generic mzXML format and converters from proprietary formats to mzXML are available for major instrument platforms Here we provide evidence that LipidXplorer ... with R = 7,500 for MS spectra (Orbitrap) and unit resolution for MS/MS spectra (IT); experiment III, four acquisitions with R = 30,000 for MS spectra (Orbitrap) and unit resolution for MS/MS spectra...

Ngày tải lên: 09/08/2014, 22:23

... nucleotide file It performs automatic IS-associated ORF identification using IS-associated transposase and transpositionrelated (for example, regulatory) gene models (provided by ISfinder) for ‘.fasta’ ... performed using ISsaga can be found on the web site without registration Selected tabs that are important for understanding the description below are indicated in the accompanying text in the form: ... more than 40 expertly annotated genomes (119 replicons) The ISsaga platform has been designed to maintain common standards for high quality IS annotation used in ISfinder at both protein and nucleotide...

Ngày tải lên: 09/08/2014, 22:24

... Alphascreen® signal indicating the formation of a highly stable ROP-R06-TAR kissing complex (Figure 3C) A ~10 fold higher AlphaScreen® signal was observed for this ternary complex in the presence of ... immobilized on the two beads [20] (Figure 2A) Therefore monitoring the emission signal allows for the detection of complex formation We used this technology for monitoring the interactions between a candidate ... M1 and M2, were performed on the automated workstation For each SELEX, μM of the RNA library was heated at 80°C for min, cooled at 4°C for min, placed at room temperature for and mixed DNA primers...

Ngày tải lên: 11/08/2014, 00:23

... as before, and polymerase chain reaction performed in a Thermo Electron (Milford, MA, USA) MBS 384 Satellite Thermal Cycler (initial denaturation of 92°C for minutes, 92°C for 30 s, 60°C for 30 ... shown) Taken together, these data indicate that LMF represents a highly accurate signature analysis method that is suitable for high- throughput applications reviews Peck et al R61.3 comment Fraction ... expression-based high- throughput screening (GEHTS) and application to leukemia differentiation Nat Genet 2004, 36:257-263 Yang L, Tran DK, Wang X: BADGE, Beads Array for the Detection of Gene Expression, a high- throughput...

Ngày tải lên: 14/08/2014, 16:21

... protein biotinylation strategies for potential high- throughput proteomics J Am Chem Soc 126, 10551062 (2004) Hu, Y., Chen, G.Y.J & Yao, S.Q Activity-based high throughput screening of enzymes using ... (2005) Hu, Y., Uttamchandani, M & Yao, S.Q Microarray: a versatile platform for highthroughput functional proteomics Comb Chem High Throughput Screen 9, 203-212 (2006) x List of Tables Table 2.1 Proteins ... from three different aspects for high- throughput identification of enzymes and their associated proteins in yeast proteome (catalomics) Firstly, to validate the high- throughput capacity of differential...

Ngày tải lên: 16/09/2015, 08:30

... likelihood of obtaining a product that is effective Unfortunately these latter viral inhibition assays are usually not suited to high throughput screening (HTS) In this report we describe modifications ... confirmed the suitability of NA activity as a practical end-point index for development of a high throughput assay with rapid read-out for inhibition of virus replication This "accelerated" virus inhibition ... virion and its activity has previously been used to quantify virus [16] For the purpose of developing a high throughput screening assay, we needed to show that NA activity is readily measured...

Ngày tải lên: 20/06/2014, 01:20

... 5'-TTGCAAGTTTGCTGCTGAAC-3' (95°C for 10 minutes followed by 40 to 50 cycles of 94°C for 15 s, 59°C for 20 s, 72°C for 30 s and 76°C for s (signals acquired)), whereas the primers for the promoter of mir-21 ... were treated with 10 μM doxorubicin (Sigma Dorset, UK) for h before being allowed to grow for another 24 h, 200 μM deferroxamine (DFX; Sigma) for 24 h, with or without μM NF-κB activation inhibitor ... 5'-TACAAACTGGGGAGCTTGGT-3' and 5'-AACCCCTGCGTCATCCTTAT-3' (95°C for 10 minutes followed by 40 to 50 cycles of 94°C for 15 s, 59°C for 20 s and 72°C for 30 s (signals acquired)) PCR signals were standardized...

Ngày tải lên: 11/08/2014, 12:20

... before detailed images of these regions can be generated A naive complete high- resolution scanning is out of the question due to the tight time constraints of a high- throughput system Therefore, ... the developed high- throughput screening system is a motorized, computer-operated light-optical microscope (left) The xy-table can carry up to slides (right) Page of (page number not for citation ... mission The desired screening of thousands of candidate genes would require many person years without automation Relegating this task to a fully automated high- throughput screening system offers...

Ngày tải lên: 12/08/2014, 05:20

... k) for models D1, the background distribution R2j (j = k) for models D2, and the background distribution Rzj (j = k) for models Dz To estimate significance of the inferred network model D1 for ... KEGGanim: pathway animations for high- throughput data Bioinformatics 24, 588–590 Antonov AV & Mewes HW (2006) Complex functionality of gene groups identified from high- throughput data J Mol Biol ... network (model 2) For example, model D5, which allows up to four intermediate compounds, covers 11 metabolites For each model, the P-value was estimated using a Monte Carlo procedure For the most...

Ngày tải lên: 16/03/2014, 01:20

... overall Kappa for the four topics were 0.59 for pulmonary fibrosis, 0.79 for cholangiocarcinoma, 0.79 for diabetes mellitus and 0.59 for congestive heart failure We ran a set of queries for each of ... 0.764487 0.769548 0.775346 Table Performance of different annotation methods The f-score results are fairly close for all methods; however, the recall is highest for the method that combines Metamap ... language models and lexicons for tagging In print in Journal of Biomedical Informatics, 2005 Pakhomov, S., A Coden, and C Chute, Developing a Corpus of Clinical Notes Manually Annotated for Part-of-Speech...

Ngày tải lên: 31/03/2014, 03:20

... Biochemical Target type Assay formats Table The Most Important Assay Formats for Various Target Types Macarrón and Hertzberg High Throughput Screening Assays able performance while keeping assay ... Lutz, M.W., Menius, J.A., et al (1996) Experimental design for high- throughput screening Drug Discov Today 1, 277 – 286 High Throughput Screening Assays 29 15 Taylor, P., Stewart, F., et al (2000) ... Saussy, D L (1996) Experimental design for high- throughput screening Drug Discovery Today 1(7), 277–286 Major, J S (1995) Challenges of high throughput screening against cell surface receptors...

Ngày tải lên: 11/04/2014, 09:46

... channel model we have considered assumes that sensors are static (or restricted to very slow movement) Our system model and the energy-efficient scheme developed in this paper may be suitable for the ... requirements for each sensor by means of SNR gap approximation We apply this approximation because it allows to relate a constant application quality (SER) with a constant throughput straightforwardly ... energy per bit is lower for M-QAM for distance less than 30 m, which is our range of interest Other related works consider also M-QAM as the multilevel modulation to be used for energy efficient communications...

Ngày tải lên: 22/06/2014, 19:20