competencies required for a sales manager
Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
Ngày tải lên :
16/03/2014, 04:20
... constants
have been reported only for AcAc-SPP [3]; for exam-
ple, K
m
and k
cat
are 73 lm and 469 ⁄ s for AcAc-SPP,
whereas they are 24 lm and 465 ⁄ s, respectively, for
AcAc-CoA. In the crystal ... mother
liquor, was allowed to continue for 4 days prior to data
collection. This experiment was performed for both a wild-
type thiolase crystal and a C8 9A mutant crystal. Analysis
of the data collected ... stirring. After
Table 3. Calorimetric analysis of CoA binding to the Z. ramigera biosynthetic thiolase at 25 °C. The values and error estimates are calculated
from separate measurements (three for...
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Effective Sales Management Techniques - A Few Important Steps can keep a Sales Manager Focused and His or Her Team Accountable doc
Ngày tải lên :
30/03/2014, 12:21
... that help is available, in fact encouraged at all times.
1
Effective Sales Management Techniques
A Few Important Steps can keep a Sales Manager Focused and His
or Her Team Accountable ...
Philadelphia, Washington, D.C., Boston, Baltimore, Miami, San Francisco,
Oakland, St. Louis, Chicago, Los Angeles and many other national and local
publications. In addition, Dan has appeared ... to answer that question, the sales manager must first look at the reporting
structure that he or she has put in place for the entire sales team.
All sales teams should meet at least twice a...
6
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Tài liệu Proposal for a DIRECTIVE OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL on Alternative Investment Fund Managers and amending Directives 2004/39/EC and 2009/…/EC ppt
Ngày tải lên :
19/02/2014, 09:20
... &apos ;manager of alternative investment funds ' or AIFM means any legal or natural
person whose regular business is to manage one or several AIF;
(c) 'Valuator' means any legal ... progress on activities
and financial affairs, report on significant events in the financial year;
(b) with regard to financial and other risks at least financial risks associated with
capital structure; ... retail
clients have to take into account these additional safeguards when assessing whether a
certain AIF is suitable or appropriate for an individual retail client. Where a Member
State allows...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
Ngày tải lên :
19/02/2014, 16:20
... sequence aaA
TGCa to aaTTCCa (core bases shown in uppercase letters)
centered at )123 within Prm3 was performed using the
mutator primers Kin175 (5¢-CACCAGAGCTACTTACA
CTGAATTCCAGAATAATCACAAGCAAATC-3¢; ... Kinsella BT, O’Mahony D, Lawson JA, Pratico D &
Fitzgerald GA (1994) Cellular activation of thrombox-
ane receptors. Ann N Y Acad Sci 714, 270–278.
56 Yamaguchi S, Murata Y, Nagaya T, Hayashi ... )118).
(6) Prm3ac; pGL3b:Prm3ac & pGL3e:Prm3ac. (Primer
Kin188; 5¢-dGAGA
GGTACCGAATTAATCACAAGCAA
ATCTTCTC-3¢, corresponding to NTs )119 to )94).
(7) Prm3aab; pGL3b:Prm3aab & pGL3e:Prm3aab. (Primer
Kin160;...
18
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Báo cáo khoa học: Lpx1p is a peroxisomal lipase required for normal peroxisome morphology potx
Ngày tải lên :
07/03/2014, 05:20
... Chemicals, Darmstadt, Germany).
For construction of pPC86-LPX1 and pPC97-LPX1,
YOR084w was amplified using primers 5¢-CCCGGGAAT
GGAACAGAACAGGTTCAAG-3¢ and 5¢-AGATCTTTA
CAGTTTTTGTTTAGTCGTTTT-3¢, and ... Woolford CA, Noble JA, Garman JD, Tam MF, Innis
MA & Jones EW (1993) Phenotypic analysis of protein-
ase A mutants. Implications for autoactivation and the
maturation pathway of the vacuolar ... (AAC71532) and with the putative triacylglycerol lipase
AAB96044 from Mycoplasma pneumoniae (Mp). Identical amino
acids are indicated by an asterisk and similar amino acids are indi-
cated by a colon and...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
Ngày tải lên :
08/03/2014, 10:20
... the transcription initiation site a TATA-
like sequence (TAAATA) is located between base pairs )28
and )23. A CCAAT-consensus box is located at )86 bp
(CCAAT), a reverse CCAAT motif lies at )825 ... stranded oligonucleotides
spanning the region from )70 to )36 of t he xMGP
promoter (5¢-GATCCAGGGGAGGGAAAACAAGGA
GATGAGGAGGTGTGGT-3¢,and5¢-GATCTACCA
CACCTCCTCATCTCCTTGTTTTCCCTCCCCTG-3¢)
as BamHI/BglII ... constructs
)180/)36TATALUC and )180/)72TATALUC were gen-
erated by PCR amplification with a common, sense
oligonucleotide ( 5¢-CG
GGATCCCAATCTGTTGCTAA
TTAGG-3¢)andthe3¢ specific oligonucleotides (5¢-GA
AGATCTACCACACCTCCTCATCTCC-3¢)...
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Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
Ngày tải lên :
15/03/2014, 10:20
... described. HA-RPTPa R554H was obtained by site-
directed mutagenesis using HA-RPTPa WT as the template
and the following forward and reverse oligonucleotides:
5¢- ATG AAG AAG AAC CAT GTT TTA CAG ATC ... demonstrate here, for the first time, that a func-
tional, catalytically active D2 is required for RPTPa to
bind to its substrate, Src, and to dephosphorylate and
activate it.
Materials and methods
Materials ... activation. To confirm
that catalytic activity of RPTPa-D2 was required for these effects, we ana-
lyzed a third mutant, RPTPa-R729K, which had an inactive D2. Again,
Src binding was reduced and...
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Used goods, not used bads: Profitable secondary market sales for a durable goods channel pptx
Ngày tải lên :
16/03/2014, 11:20
... Canan Savaskan, David Soberman, Miguel Villas-Boas,
Rajiv Lal and an anonymous reviewer for helpful comments on an earlier draft.
References
Blair, R. D., & Lafontaine, F. (1999). Will Khan ... serve as a gateway for future empirical tests and theoretical generalizations.
Acknowledgements The authors thank Fabio Caldieraro, Gregory Carpenter, Preyas Desai, Benjamin
Handel, Karsten Hansen, ... 4 a ÞðÞ
a 8þ 5a Þþ4q 1 a Þ
hi
a gaþqðÞ
3a 2q
1ÀcþgaþqðÞ
21 a qðÞ
c g; a; qðÞ1 þ a À g þ
a 1þgðÞ
2 2a qðÞ
þ
2 a Þ2þgðÞ
6a 4q
and
e
c g; a; qðÞ
a 5À2gþ 2a agðÞþq 2À2gþ 2a agðÞ
3a 2q
. For c < c,
some...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx
Ngày tải lên :
16/03/2014, 13:20
... 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢
Vps4 Ter2 F 5¢-TTAAAAGAACCAGATTAGTCAATTGATTAACGTGCT-3¢
Vps4 Ter2 R 5¢-AGCACGTTAATCAATTGACTAATCTGGTTCTTTTAA-3¢
Vps4 RDE F 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢
Vps4 ... 5¢-GCCCATATTCGTCGACGCGCTAACAGGTACCAGAGGAGAAGGAGAGAGCGAAGCAAGTAG-3¢
Vps4 Dstr R 5¢-GGGCGGATCCTCTGCTTTTCTTTATC-3¢
Vps4 Ter1 F 5¢-GCGCTAATGCAACCGTAGTCAATTGATTAACGTGCT-3¢
Vps4 Ter1 R 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢
Vps4 ... 5¢-GACGACGAAACAAGAAAAGATGGCGCCATCGAGATG-3¢
Vps4 LTP R 5¢-CATCTCGATGGCGCCATCTTTTCTTGTTTCGTCGTC-3¢
Vps4 GAI F 5¢-TGCTCTCCAGGTGATGATATTGAAGCTGATGAATTA-3¢
Vps4 GAI R 5¢-TAATTCATCAGCTTCAATATCATCACCTGGAGAGCA-3¢
P....
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Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot
Ngày tải lên :
23/03/2014, 07:20
... 5¢-AAGGTGCAGAAGACGCA
CTT-3¢; cycE-R, 5¢-AATCACCTGCCAATCCAGAC-3¢;
cdk4-F, 5¢-TACAACAGCACCGTGGACAT-3¢; cdk4-R,
5¢-TGGGCATCGAGACTATAGGG-3¢; rp49-F, 5¢-CGG
ATCGATATGCTAAGCTG-3¢; and rp49-R, 5¢-GAACG
CAGGCGACCGTTGGGG-3¢.
Acknowledgements
We ... Invitrogen for 2 h at 25 °C.
DNA was visualized with DAPI. Preparations were mounted
in FluoroGuard Antifade Reagent (Bio-Rad, Hercules, CA,
USA), and images were obtained using an Olympus (Tokyo,
Japan) ... 400) for 16 h at 4 °C, and this was followed by incuba-
tion with Alexa-488-conjugated anti-rabbit IgG (1 : 400)
for 2 h at 25 °C. DNA was stained with DAPI.
Semiquantitative RT-PCR
Total RNA was...
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Selling Financial Products - A proven methodology for increasing sales of banking and financial services doc
Ngày tải lên :
23/03/2014, 11:20
... techniques after all these years”
Selling Project Finance Services – Asian bank
■ ABSA
■ Alpha Bank
■ Axa Investment Managers
■ Bank BPH SA
■ Bank of America
■ Bank of China
■ Bank of Kuwait and the ... Rabobank
■ Rand Merchant Bank (SA)
■ Rating Agency Malaysia
■ Raiffeisen International and RZB
■ Saudi Arabian Monetary Agency
■ Shell
■ Société Générale
■ Standard Chartered Group
■ State Bank ... the Middle East
■ Bank Pekao SA
■ Bank Zachodni WBK SA
■ BBVA Group
■ BNP Paribas
■ Calyon
■ Central Bank of Kuwait
■ Caixa Geral de Depositos
■ China International Capital Corporation
■ Citigroup
■...
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Management in India: Grow from an Accidental to a Successful Manager in the IT & Knowledge IndustryA real-world, practical book for a professional in his journey to becoming a successful manager in IndiaRahul Goyalprofessional expertise distilled doc
Ngày tải lên :
23/03/2014, 13:20
... sides. A good team and a good upper management
are both necessary for a manager to contribute.
The hierarchy or leader of the pack
Most people tend to think of a manager primarily as a leader and ... inuences and dilemmas. As a manager, you
are destined to face these dilemmas on a daily basis. Managerial decision-making
depends on more than just the immediate facts of the task at hand. It's ... information and keeping an adequate ow of information, so that teams
don't rely on the grapevine and look towards their managers to get the information.
Spokesperson
A manager speaks for...
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Báo cáo khoa học: A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis docx
Ngày tải lên :
29/03/2014, 09:20
... [8–13] and
Arabidopsis thaliana [14,15]. Yeast, flies, nematodes
and mammals encode a single SPO11; however, plants
(e.g. Arabidopsis and rice Oryza sativa) encode at least
three SPO11 paralogues ... insertional
mutagenesis of Arabidopsis thaliana. Science 301, 653–
657.
43 Clough SJ & Bent AF (1998) Floral dip: a simplified
method for Agrobacterium-mediated transformation of
Arabidopsis thaliana. ... Jasco spectropolarimeter, model J-720
(Jasco, Tokyo, Japan).
Complementation of a homozygous mutant of
A. thaliana spo11-1
A T-DNA insertional mutant (spo11-1-3)ofA. thaliana
SPO11-1 (SALK_146172:...
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Báo cáo khoa học: Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system potx
Ngày tải lên :
30/03/2014, 14:20
... Naar, A. M., Beaurang, P .A. , Zhou, S., Abraham, S., Solomon,
W. & Tjian, R. (1999) Composite co-activator ARC mediates
chromatin-directed transcriptional activation. Nature 398,832.
18. Naar, ... RNAPII, Medi-
ator and GTFs.
Purification of the Gal4 DNA binding domain, Gal4-AP2,
Gal4-VP16 and GAL4-CTF
The gene that encodes the Gal4-AP2 transcriptional
activator, which contains the Gal4 ... reconstituted assay using
the RNAPII holoenzyme and found that Gal4-CTF is able
to activate basal transcription (Fig. 5E, compare lanes 1, 2,
and 3) whereas Gal4-SP1 is not (compare lanes 1, 4, and 5).
Antibodies...
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ENHANCING THE COMMUNICATION SKILLS OF EDUCATIONAL MANAGERS IN VINH PHUC PROVINCE: BASIS FOR A TRAINING PROGRAM
Ngày tải lên :
13/05/2014, 15:15
... in management, communication has the
characteristics of management, but it retains its basic characteristics. Characteristics
of communication in management have peculiarities, valuable management ... the management. To establish relationships with management
objects, to deal with management tasks, to maintain management functions,
management staff should have the psychological traits and ...
are many ways to look) and judgment, thinking generalization, abstraction all
information received. Therefore, awareness of communication is an art. There are
even people concealing information...
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