comparison with mlva method coupled with gel electrophoresis
Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
Ngày tải lên :
16/02/2014, 15:20
... brief, membrane proteins were solubilized with various types of detergents at high concentrations and subsequently incorporated into polyacrylamide gels without electrophoresis Excess detergent was ... solution The gel was cut into small pieces and washed several times with mL of triethylammonium bicarbonate containing 50% (v ⁄ v) acetonitrile The gel samples were further dehydrated with 100% ... performed with trypsin (protein ⁄ trypsin = 10 : 1, g ⁄ g) in 25 mm triethylammonium bicarbonate with incubation overnight at 37 °C Peptides were extracted from the gel using sequential extraction with...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
Ngày tải lên :
16/03/2014, 16:20
... Tris/HCl pH 6.8 with heating at 50 °C for 15 before electrophoresis on 10% Tris/tricine gels Immunoblotting For analysis of PS1, NCT, PEN-2 and APH-1, membrane proteins were separated with reducing ... urea indicated disruption of PS1 complex With 0.1% SDS, PS1 was detected as bands of 90 and 60 kDa With 0.1% SDS, NCT was detected largely as monomer with minimal reactivity corresponding to ... on 4–16.5% gradient acrylamide gels with cooling; anode buffer contained 25 mM imidazole pH 7.0; cathode buffer contained 50 mM tricine, 7.5 mM imidazole pH 7.0 with 0.02% Coomassie G 250 [34]...
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Báo cáo khoa hoc:" Retroperitoneoscopic radical nephrectomy with a small incision for renal cell carcinoma: Comparison with the conventional method" docx
Ngày tải lên :
11/08/2014, 07:21
... from the A method to the B method During this period, the A method was performed in cases while the B method was performed in cases A total of six surgeons handled these cases, and the A method was ... radical nephrectomy with a small incision for renal cell carcinoma: Comparison with the conventional method Journal of Negative Results in BioMedicine 2011 10:11 Abbreviations A method: Retroperitoneoscopic ... proved that these methods are less invasive than open surgery The A method was similar to this hand-assisted laparoscopic surgery But the A method could be better than hond-assited method in terms...
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Báo cáo y học: "Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis" pps
Ngày tải lên :
12/08/2014, 04:20
... homogeneous SDS-PAGE gels (24 cm × 20 cm × mm) casted with low-fluorescence glass plates utilizing Hofer SE 600 (GE Amersham) The SDS-PAGE gels were run at 15 mA /gel for 20 and then at 30 mA /gel at 15°C ... unbiased results 2-D gel electrophoresis Following the labeling reaction, 50 μg of each Cy2, Cy3 and Cy5 labeled samples were mixed Then the pooled sample of each gel was diluted with rehydration ... recommendations Briefly, Differential in gel analysis (DIA) module was used for intra -gel analysis for protein spot detection and for normalization of Cy3 and Cy5 gel images with respect to the Cy2 image...
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Báo cáo y học: "Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery" docx
Ngày tải lên :
13/08/2014, 13:20
... here a promising alternative method for the direct and non-invasive sampling of oral lesions, which can be coupled with mass spectrometry (MS)-based proteomics Our method uses commercially available ... here a promising new method for the non-invasive, direct sampling of oral lesions, compatible with MS-based proteomics In future studies, we envision application of this method to comparative ... article as: Kooren et al.: Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery Clinical Proteomics...
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Báo cáo y học: "Usefulness of manufactured tomato extracts in the diagnosis of tomato sensitization: Comparison with the prick-prick method" pdf
Ngày tải lên :
13/08/2014, 13:22
... same trained person, unaware of clinical data Statistical analysis A within subject comparison design between prick-prick test with fresh ripe Canary tomato, considered as a comparator, and manufactured ... Clinical symptoms with tomato and sensitizations of the subjects from Group I (n = 26), according to the positivity of the prick-prick test with tomato SPT + n Age Sex Atopy Symptoms with tomato Anaphylaxis ... compared with other varieties [16] There are no studies comparing manufactured prick tests and prickprick tests with fresh tomatoes, but our results replicate those found by several authors with...
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Idioms with as as in comparison with the vietnamese ones = nghiên cứu thành ngữ so sánh trong tiếng anh và tiếng việt
Ngày tải lên :
19/12/2013, 10:39
... comparative idioms with as as referring to people and their equivalents in Vietnamese Methods of the study - Statistic method - Contrastive and comparative method - Analytic and synthetic method Design ... small scale of the study, we only deal with comparative idioms with as as about the appearance, character, mental state, and health state in comparison with the Vietnamese ones Aims of the study ... study Scope of the study Methods of the study Design of the study Part B: Investigation Chapter 1: Idioms Chapter 2: Idioms with as as referring to people in comparison with the Vietnamese ones...
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Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt
Ngày tải lên :
08/03/2014, 10:20
... cells first primed with GM-CSF Fig Comparison of the effect of W7 and LY294002 on the respiratory burst in human neutrophils Cells were isolated and treated without (open symbol) and with 10)10 M GM-CSF ... were rescued from apoptosis after treatment with GM-CSF IL-8 treated cells, however, showed no additional survival compared with the untreated neu- Fig Comparison of the effects of cytokines and ... and pretreated with W7 (50 lM) or LY294002 (20 lM) for 10 Neutrophils were allowed to attach to the coverslip for 10–15 at 37 °C Medium was removed and the cells were washed twice with Hepes buffer...
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Báo cáo khoa học: "Framework of Semantic Role Assignment based on Extended Lexical Conceptual Structure: Comparison with VerbNet and FrameNet" ppt
Ngày tải lên :
24/03/2014, 03:20
... are also annotated with a Source, and Effectors of movement verbs are sometimes annotated with Source or Goal contain a phonogram form (Hiragana form) of a certain verb written with Kanji characters, ... function, we can express complicated events with predicate decomposition and prevent missing (multiple) roles A key point for associating LCS framework with the existing frameworks of semantic roles ... Frame-independent yes yes no # of roles 13 30 8884 Table 6: Number of roles in each resource Comparison with other resources 4.1 Number of semantic roles The number of roles is related to the number...
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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
Ngày tải lên :
31/03/2014, 23:20
... proportional phase increment (TPPI) method [31] or the states-TPPI method The carrier frequency was set on the H2O resonance NOESY experiments were recorded with mixing times of 50 and 200 ms ... sensitive mode using TPPI method Decoupling during acquisition with a GARP sequence and presaturation during relaxation delay (1.6 s) were used A total of 256 FIDs with 128 transients were collected ... MATERIALS AND METHODS Protein synthesis The entire Vpr protein was synthesized on an Automatic Applied Biosystems 433 A peptide synthesizer using the stepwise solid phase synthesis method and Fmoc...
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báo cáo hóa học: " Is global quality of life reduced before fracture in patients with low-energy wrist or hip fracture? A comparison with matched controls" docx
Ngày tải lên :
18/06/2014, 19:20
... 21:190-195 Burckhardt CS, Archenholtz B, Bjelle A: Quality of life of women with systemic lupus erythematosus: a comparison with women with rheumatoid arthritis J Rheumatol 1993, 20:977-981 Flanagan JC: ... patients and 456 hip fracture patients with a low-energy fracture were treated at the hospital; 249 of the patients with a wrist fracture and 307 of those with a hip fracture were examined at the ... patients with lowenergy wrist and hip fractures and to compare the scores with age-and sex-matched controls The hip fracture patients reported lower GQOL before the fracture occurred compared with...
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báo cáo hóa học: " The health-related quality of life in rheumatoid arthritis, ankylosing spondylitis, and psoriatic arthritis: a comparison with a selected sample of healthy people" potx
Ngày tải lên :
18/06/2014, 19:20
... with IRD invited to undergo a complete medical history, a careful clinical examination and radiological evaluation, 799 (71.3%) patients (469 with RA, 164 with SA, 65 with axial PsA and 101 with ... the patients with RA and PsA and those with AS are significantly different (p < 0.001) Slightly more than one quarter of the patients with RA, more than two thirds of the patients with AS, and ... patients with PsA (44.6 with peripheral PsA and 49.3 with axial PsA) were male The onset of AS is typically earlier than RA; therefore, in older age-matched healthy controls, the patients with AS...
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Báo cáo hóa học: "Case-Control study of Firefighters with documented positive tuberculin skin test results using Quantiferon-TB testing in comparison with Firefighters with negative tuberculin skin test results" potx
Ngày tải lên :
20/06/2014, 00:20
... and transported to the lab within the specified period of time For this study, the Maricopa County Laboratory performed the testing in accordance with manufacturer's methodologies Results were ... positive results, indicates that there should be less of an issue with false positive responses using QFT, even though comparison with TBST can only tell that the rates are significantly different ... the 220 controls (firefighters with no history of a positive TBST), (3.2%) were positive and 10 (4.5%) resulted in conditionally positive responses Figure shows the comparison of the study cases...
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báo cáo hóa học:" Assessment of the primary rotational stability of uncemented hip stems using an analytical model: Comparison with finite element analyses" potx
Ngày tải lên :
20/06/2014, 01:20
... distinguish between stems with sufficient and insufficient primary stability; new stem designs with an antirotation value lower than this threshold could then be dismissed without the need for measurements ... without the need for FEM A parameter characterising the potential primary stability of a hip stem could also be of great value in pre-operative planning of THRs It could provide the surgeon with ... stem with optimal fit and fill of the intramedullary canal will resist the daily loads on the hip better than standard stems [28] However, a large variety of stem geometries is obtained with...
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báo cáo hóa học:" Health-related quality of life in urban surgical emergency department patients: Comparison with a representative German population sample" docx
Ngày tải lên :
20/06/2014, 15:20
... care patients in USA) found impaired HRQoL in comparison to patients with lower severity of alcohol dependence, a normal population, and patients without alcohol disorders, respectively In addition, ... Score with mental health were male gender, a yearly income above 24,000€, and living in a more-person-household In this investigation, injuries with more than ISS-point were associated with a ... authors reported no findings on HRQoL, all of these reported conditions associated with frequent ED-use are associated with impaired HRQoL EDs play an important role in alcohol/drug abuse screening...
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Estimation of uncertainty in three dimensional coordinate measurement by comparison with calibrated points ppt
Ngày tải lên :
20/06/2014, 18:20
... blocks in two orientations, with each end wrung to the platen in turn We have not made a systematic study with this method but we have some data gathered in conjunction with international interlaboratory ... are calibrated by comparison to calibrated master flats The master flats are calibrated using the three-flat method, which is a self-calibrating method [22] In the three flat method only one diameter ... compared calculations from Puttock and Thwaite with other published calculations, particularly with NBS Technical Note 962, “Contact Deformation in Gage Block Comparisons” [12] and NBSIR 73-243, “On...
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Báo cáo hóa học: " Performance analysis for optimum transmission and comparison with maximal ratio transmission for MIMO systems with cochannel interference" pdf
Ngày tải lên :
21/06/2014, 00:20
... precise CCI model is used, the BER with (T, R) = (3, 2) is slightly better than that with (T, R) = (2, 3), whereas the BER with (T, R) = (2, 4) is better than that with (T, R) = (4, 2) in our test ... This article studies the average BER of quadrature amplitude modulation (QAM) with OT and provides the comparison with MRT using the precise CCI model when the desired signal and interferers are ... performance of OT with (T, R) = (2, 2) is worse than that with (T, R) = (1, 3) in the case of L = due to noise enhancement discussed in the nonfading case However, the performance with (T, R) = (2,...
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Báo cáo lâm nghiệp: "Evaluation of the growth and health status of selected clone mixtures in comparison with ordinary planting stock" docx
Ngày tải lên :
07/08/2014, 03:22
... microsites within one locality was also considerable The observed large intraclonal differences are consistent e.g with data of Johnsen and SkrØppa (1992), who observed high variability of growth within ... (ANOVA) was used to test the differences due to provenance of clones within in all studied characteristics Subsequently, paired comparisons of pairs of the clone progenies were done by Scheffé’s ... in the progeny of clone No 548 again A comparison of the growth of rooted cuttings (2nd generation clones) and control planting stock produced by a routine method shows the relatively good growth...
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Báo cáo khoa học: "Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains" pps
Ngày tải lên :
07/08/2014, 18:21
... fo noitacifilpma dna noitalosi eht dewolla snoitcaer RCP ni desu sremirP noitcaer RCP )ecnarF ,neGelliM( senolc desab-T2-XEGp evitisop eerht fo sdnarts owt eht no tuo deirrac saw eneg eht fo gnicneuqeS...
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Báo cáo lâm nghiệp: "Utilization of a dynamometric pendulum to estimate cutting forces involved during routing. Comparison with actual calculated values" pdf
Ngày tải lên :
08/08/2014, 00:22
... and with one of current formulations [8] precision, and better representation of wood material influences (wood characteristics, anatomy, …) Then, in comparison with the first method obtained with ... it is something already explained with wood anatomy in a previous work [7] A comparison between results obtained with pendulum (Fig 8), and results obtained with one of current formulations (using ... obtained with new formulation (Eq (6)); Vr is the removed chip volume during routing; Vpend is the removed chip volume with pendulum There are clearly some differences between measured values, with...
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