... examples in which the ECM proteins laminin, fibronectin, and collagen have been used for nerve repair applications [12,18-27] For example, silicone tubes filled with laminin, fibronectin, and ... sciatic nerve injury For this test, the entire body of the rat, excepting the hind-limbs, was wrapped in a surgical towel Supporting the animal by the thorax and lowering the affected hind-limb towards ... push-off sub-phase In clear contrast to the normal pattern of ankle movement, at week-2 post-injury animals were unable to extend this joint and dorsiflexion continued increasing during the entire...
Ngày tải lên: 19/06/2014, 08:20
... skin testing performed a the PFDHC follows a specific testing protocol, by health personnel trained in proper Mantoux intradermal injection techniques, and with objective reading of the skin ... their individual test results In addition, the principle investigator, obtained the following information from the Health Center database: Year of Birth, Year of Hire, date of positive TBST (in ... to: 1) compare QFT results to TBST results in a population where a high incidence of positive LTBI results are present; and 2) determine if MA is a confounder in TBST testing among our firefighters...
Ngày tải lên: 20/06/2014, 00:20
Idioms with as as in comparison with the vietnamese ones = nghiên cứu thành ngữ so sánh trong tiếng anh và tiếng việt
... English idioms with as as referring to people in comparison with those in Vietnamese We hope that this will be an interesting and useful teaching material for everyone who has interest in English ... relationship in the society 1.2 History of studying idioms I.2.1 In England and Europe In England and Europe, lots of linguists started their study in English idioms since the very beginning of 20th ... language? Were laying it on thick Were laying it on thin, too In fact, we can laminate in plastic from mm to 60 mm thickness So call us Well stick with you through thick or thin Lamination Concepts...
Ngày tải lên: 19/12/2013, 10:39
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... aminoglucose in a-glycosidic linkage or the natural sugar N-methylfucosamine in b-glycosidic linkage to the backbone, were found to interfere with bulge-specific cleavage by NCS-chrom and to inhibit ... ligand–nucleic acid complex is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B CD spectroscopy ... overhanging nucleotides were excised completely from the 3¢-terminus in the annealing oligomer, and consequently, no expanded band was observed during incubation Doxorubicin did not inhibit the...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... chemotherapy Indeed, it has been shown that a palindromic trioctamer of this sequence can interfere with CREB binding, and specifically inhibit PKA subunit expression, interfering with the CRE-PKA ... containing 10 lM BrdU for 30 mins before fixing with ice-cold 70% (v/v) ethanol and permeabilization in M HCl with 0.5% (v/v) Triton X-100 Samples were washed and incubated with fluorescein isothiocyanate-conjugated ... combining CDO with cytotoxic agents in HCT116 and GEO cells Cells were cultured with CDO (C) alone or in combination with 5-FU, SN38 or VP16 There were significant reductions in cell number in...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt
... calmodulin and PtdIns3K In guinea pig neutrophils, it has been demonstrated that inhibition of calmodulin results in inhibition of Rac and p21 activated kinases [50] Involvement of Rac in ROS ... switch of a5b1 integrin in CHO cells, suggesting also a role for Ca2+/calmodulin-dependent molecules in regulating the affinity state of integrins on neutrophils [48] Although PtdIns3K has been ... in 0.5% human serum albumin The assay was performed in Hepes buffer supplemented with 0.5% human serum albumin for 1.5 h at 37 °C in a CO2 incubator Filters were fixed, stained with hematoxilin...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Concerted mutation of Phe residues belonging to the b-dystroglycan ectodomain strongly inhibits the interaction with a-dystroglycan in vitro pot
... processing and targeting of DG in vivo Results Mutations within the b-DG(654–750) recombinant fragment The phenylalanine in position 718, belonging to the putative a-DG-binding epitope (691–719) within ... staining, cells were incubated overnight with a polyclonal antibody directed against the C-terminus of a-DG [20] diluted : 150 in NaCl ⁄ Pi containing 3% (w ⁄ v) BSA For intracellular staining, ... 691 and 719 [15] In order to obtain a deeper insight into the a–b interface, we have introduced a series of mutations within the b-DG ectodomain located in three different protein regions: FEBS...
Ngày tải lên: 16/03/2014, 12:20
Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... reported for intercalation and minor-groove binding, indicating minor involvement of DNA bases [16] However, the absence of a comparable inhibitory effect at GC-rich random sequences indicates a ... minor-groove, in the binding of DAPI [16] In conclusion, the main cause of the strong inhibitory effect of DAPI on DNA replication in vitro reported in this work appears to be linked to the propensity ... sequences within introns can regulate transcription [19,20], the prevalent location of ATT triplet repeats within introns in the human genome [18] suggests strongly that binding of DAPI to ATT-bearing...
Ngày tải lên: 16/03/2014, 23:20
Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
... used in normal reactions and varying concentrations of KCl (0–800 mM) UV cross-linking assays UV cross-linking in solution was performed on ice by exposing the 20 lL binding reaction mixture in ... (fold) besides being the most specific, interacted with a duplex DNA with a 3¢ G-overhang, a feature shared with other single-stranded telomere binding proteins [20,40] However, the binding activities ... other kinetoplastid sequences annotated as Rpa1, lacked the N-terminal domain (data not shown) that in other eukaryotes is involved only in Rpa–protein interactions and has no function in binding...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
... suggested that these findings could be explained by an interaction between the N- and C-terminal domains in the intact Vpr protein resulting in steric hindrance in the C-terminal recognition motif ... observed in structural motifs that involve protein–protein, protein–lipid or protein–nucleic acid interactions The incorporation of Vpr into the viral particles requires its interaction with a leucine ... this amino acid is included in a less structured domain This result is in agreement with the NMR determined structure in which Leu39 (preceding the second (40–48) a helix) is not involved in any...
Ngày tải lên: 31/03/2014, 23:20
báo cáo hóa học:" Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro" pdf
... cultured with differentiating human DMD myoblasts and myotubes even when the co-culture was maintained in growth media [36] The present results of co-culture of CD34+ and DMD myoblasts without the inductive ... tissue in vivo may differ from the observed in vitro Conclusion Our findings showed that umbilical cord blood CD34+ stem cells have the potential to interact with dystrophic muscle cells restoring ... dystrophin expression of DMD cells in vitro Although utilized within the context of DMD, the results presented here may be valid to other muscle-related cell therapy applications Competing interests...
Ngày tải lên: 18/06/2014, 15:20
báo cáo hóa học: " Valuation of transfusion-free living in MDS: results of health utility interviews with patients" ppt
... Living in transfusion independence 76 (16) 0.85 (0.15) Living with reduced transfusion burden 51 (16) 0.77 (0.24) Living with transfusion dependency 30 (16) 0.65 (0.29) Living in transfusion independence ... (0.22) Living with transfusion dependency 44 (18) 0.56 (0.34) Living in transfusion independence 78 (10) 0.80 (0.23) Living with reduced transfusion burden 52 (13) 0.75 (0.21) Living with transfusion ... transformation into acute myelogenous leukemia (AML)[1] Debilitating symptoms of MDS include fatigue, pallor, infection, and bleeding; with commonly associated laboratory findings of anemia,...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học: " Is global quality of life reduced before fracture in patients with low-energy wrist or hip fracture? A comparison with matched controls" docx
... have a modest decrease in health-focused quality of life within physical domain and scores in accordance with controls within http://www.hqlo.com/content/6/1/90 mental domain assessed up to two ... values of the regression coefficients were interpreted according to Cohen's effect size index, in which coefficients in the range 0.2–0.5 are defined as indicating a small difference, 0.5–0.8 a moderate ... Helden S, Cals J, Kessels F, Brink P, Dinant GJ, Geusens P: Risk of new clinical fractures within years following a fracture Osteoporos Int 2006, 17:348-354 Fayers PM, Machin D: Quality of life: the...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học: " The health-related quality of life in rheumatoid arthritis, ankylosing spondylitis, and psoriatic arthritis: a comparison with a selected sample of healthy people" potx
... emotional health, social functioning, and vitality The SF36 scores decreased (indicating a linear decline in HRQL), especially in the physical dimension, with increasing age in all categories of IRD ... become increasingly important in decisions regarding resource allocation, intervention design, and pharmacological treatment with biologic agents of individuals with chronic inflammatory disabling ... (including biological agents) with the goal of preventing or slowing permanent structural joint damage and longterm disability in IRD is the accurate monitoring of HRQL in daily practice and in...
Ngày tải lên: 18/06/2014, 19:20
Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... lymphocytes with genes encoding chTCRs to combat experimental tumors in mice with, in practical terms, limited therapeutic success The major problems encountered, including in our own preliminary experiments, ... system Binding of antigen to the TCR complex results in a cascade of events commencing with phosphorylation of critical domains of the receptor by membrane-associated Src family of protein tyrosine ... Fyn they can bind SH2 domains of other kinases, especially the zeta associated protein, ZAP-70 The ensuing events lead ultimately to transcription of genes, including interleukin-2, that promote...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... lymphocytes with genes encoding chTCRs to combat experimental tumors in mice with, in practical terms, limited therapeutic success The major problems encountered, including in our own preliminary experiments, ... system Binding of antigen to the TCR complex results in a cascade of events commencing with phosphorylation of critical domains of the receptor by membrane-associated Src family of protein tyrosine ... Fyn they can bind SH2 domains of other kinases, especially the zeta associated protein, ZAP-70 The ensuing events lead ultimately to transcription of genes, including interleukin-2, that promote...
Ngày tải lên: 20/06/2014, 04:20
báo cáo hóa học:" Health-related quality of life in urban surgical emergency department patients: Comparison with a representative German population sample" docx
... patients in the surgical ED in a university-based hospital in Berlin, Germany, were included in this investigation Survey data presented in this paper were obtained as part of a prospective intervention ... ISS = Injury Severity Score with mental health were male gender, a yearly income above 24,000€, and living in a more-person-household In this investigation, injuries with more than ISS-point were ... role in unintentional injuries Screening and brief intervention programs on substance use in Emergency Departments should consider this finding and appropriate health promoting strategies focusing...
Ngày tải lên: 20/06/2014, 15:20
báo cáo hóa học:" Valuation of transfusion-free living in MDS: results of health utility interviews with patients" ppt
... Living in transfusion independence 76 (16) 0.85 (0.15) Living with reduced transfusion burden 51 (16) 0.77 (0.24) Living with transfusion dependency 30 (16) 0.65 (0.29) Living in transfusion independence ... (0.22) Living with transfusion dependency 44 (18) 0.56 (0.34) Living in transfusion independence 78 (10) 0.80 (0.23) Living with reduced transfusion burden 52 (13) 0.75 (0.21) Living with transfusion ... transformation into acute myelogenous leukemia (AML)[1] Debilitating symptoms of MDS include fatigue, pallor, infection, and bleeding; with commonly associated laboratory findings of anemia,...
Ngày tải lên: 20/06/2014, 16:20
Estimation of uncertainty in three dimensional coordinate measurement by comparison with calibrated points ppt
... Sphere in contact with an internal cylinder (for example, plain ring gages) Cylinders with axes crossed at 90Њ (for example, cylinders and wires) Cylinder in contact with a plane (for example, cylinders ... approximate the uncertainty by taking the two end points on the calculated uncertainty curve and use the straight line containing those points as the uncertainty In this example, the uncertainty for the ... analyzing the change in the fringes (fringe counting displacement interferometer) The major sources of uncertainty—those affecting the actual wavelength— are the same for both methods The uncertainties...
Ngày tải lên: 20/06/2014, 18:20
Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
... cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing 10% FCS, 100 U/ml of penicillin, and 100 μg/ml of streptomycin ... washed with PBS and fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), ... mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were divided into four groups with seven mice in each...
Ngày tải lên: 21/06/2014, 03:20