comparison of sperm washing swim up and density gradient centrifugation methods

... Guerino Macedo and Emílio César Martins Pereira Chapter Sperm Preparation Techniques for Artificial Insemination - Comparison of Sperm Washing, Swim Up, and Density Gradient Centrifugation Methods Ilaria ... on heat, time and number of insemination, method of collecting and qualifying semen to use, level of dilution, kind of extender and the size of inseminating dose, method and use of oestrus synchronisation, ... reorganisation and final selling of the farm and its sheep happened in 2009 and 2010, and it is currently a much smaller private farm Farm code 10 11 No and name of breeds kept No of years 1: Awassi and...

Ngày tải lên: 28/06/2014, 05:20

... maturity and morphology of human spermatozoa selected by Percoll density gradient centrifugation or swim- up procedure J Reprod Fertil 1988, 84, 551-556 Lessley BA, Garner DL Isolation of motile spermatozoa ... serum albumin (BSA) to evaluate sperm quality and to use as 100 μL-droplets of spermatozoa for IVF Evaluation of sperm For evaluation of progressive motile sperm, 10 μL of diluted semen was placed ... integrity of bull spermatozoa separated with a Sephadex ion-exchange column Theriogenology 1997, 47, 845-856 Bollendorf A, Check JH, Katsoff D, Lurie D Comparison of direct swim- up, mini-Percoll, and...

Ngày tải lên: 07/08/2014, 23:22

... ejaculation volume (VOL), spermatozoon motility (MO), sperm concentration (CO) and total number of spermatozoon in ejaculate (NT), rate of abnormal spermatozoon (R) and pH of semen (pH) VOL was determined ... Summer, Autumn and Winter) were the only effects included in the model Table Results of the effect of genetic group (PiDu 25, 50 75, PiCC and PiCT) and season on growth performance and semen quality ... coefficient of determination 219 Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Table Production performance, Least square means (LSMSE) of PiDu and...

Ngày tải lên: 28/08/2013, 11:59

... intensities of artificially membrane-ruptured and un-ruptured spermatozoa and NR % There was a significant boar effect on sperm assessment values of PI, H258 and H342 - stained spermatozoa (P < 0.05) and ... intensities of Hoechst and PIstained spermatozoa in relation to litter size of multiparous farrowings Materials and methods Experimental design Semen from 19 boars, of which were of Finnish Landrace and ... divided into four groups according Blom [18], that is: normal spermatozoa, spermatozoa with major sperm defects (abnormalities of sperm head and acrosome, coiled sperm tail, etc.), spermatozoa with...

Ngày tải lên: 12/08/2014, 18:22

... production and processing of organic fruit, juice, and beverages are detailed in Chapter The processing and packaging of juices and beverages are discussed in Chapters 4, 9, and 10, and cleaning and ... create signiÞcant changes in the genetic makeup of plants and animals due to the random recombination and sorting of thousands of genes As a result of intervention by people, the hybrid seed corn ... Systems (SCS) of Oakland, California and Primus Labs of Santa Maria, California Both of these operations have actively worked with growers and packers in California and Mexico and have assisted...

Ngày tải lên: 07/10/2012, 16:50

... HOMA-IR, CRP and adiponectin) of baseline and final values and of the change on each variable in each group .a Time-by-group interactions were assessed by repeated measures ANCOVA of baseline and final ... coefficient of variation for repeated measures at baseline was less than 10% Upper and lower body strength at baseline and 16 weeks was calculated as the sum of 1RM measures for each upper and lower ... choices and physical activity, and compliance with medications and doctor’s visits [24] Subjects in this group received phone calls every other week and came to the HNRCA for testing at baseline and...

Ngày tải lên: 31/10/2012, 15:28

... goods, services, projects and processes Most Six Sigma companies make use of spreadsheets and databases to collect, analyze, and track results Both standard software packages and tailor-made systems ... to changing conditions, and the application of new theories and methods It is a firm belief in the progress of human beings The first paragraph refers to the utilization of production elements, ... projects are supposed to focus on improvement of customer satisfaction which eventually gives increased market share and revenue growth As a result of revenue growth and cost reduction, the profit increases...

Ngày tải lên: 07/02/2013, 09:46

... of correct maintenance planning and scheduling procedures there is often a vast and rapid change in the understanding of what is required of the maintenance resources from week to week This often ... acquired control of 16 coal mines and about 700,000 acres of timberland, built a sawmill, acquired a fleet of Great Lakes freighters to bring ore from his Lake Superior mines and even bought a ... (Mistake or Error Proofing) • Use of JIT and Pull (Supplying items JIT [Just-in-Time] and Pulling items only as you need them) Not all of these terms (and perhaps none of them) may be familiar to you;...

Ngày tải lên: 23/11/2013, 14:10

... ourselves, but we obligate our suppliers and contracting parties in the context of possibilities to the adherence of our environmental standards By a systematic set of rules and regular examinations ... information- and communication organization Apart from the promotion of the quality and environmental awareness of our coworker, the optimization of the work surrounding field is a substantial function of ... We evaluate and monitor the impact of our activities on the environment and check in advance the possible influences of new products and procedures on the environment Already by and during the...

Ngày tải lên: 12/12/2013, 00:15

... addition, complications of pregnancy increase for both the mother and the offspring with advanced maternal age.6 Women and their health care providers should be aware of the effects of age on reproductive ... representation of the number of primordial follicles present in the ovaries and the chromosomal quality of oocytes in relation to female age and corresponding reproductive events Proportion of poor ... study of almost 3000 cycles showed cumulative pregnancy rates of 62% for women

Ngày tải lên: 13/02/2014, 16:20

... radio, and newspapers and some national media (such as USA Today, The Weather Channel, and CNN) provide daily air quality reports, often as part of the weather forecast Here’s the type of report ... your local media, and on many state and local telephone hotlines You can also sign up to receive AQI forecasts by e-mail: • AQI on the Internet EPA and its federal, tribal, state, and local partners ... Ozone levels often peak in the afternoon to early evening Carbon monoxide may be a problem during morning or evening rush hours And particle pollution can be high any time of day, and is often elevated...

Ngày tải lên: 17/02/2014, 22:20

Ngày tải lên: 18/02/2014, 11:20

... names and addresses of national consumer organizations that offer support to people experiencing infertility Success rates can be reported in a variety of ways, and the statistical aspects of these ... group of >42 are now presented in two categories: 43–44, and >44 •  Individual Fertility Clinic Tables: Summary statistics for the age group of >42 are now presented in two categories: 43–44, and ... ercentage of ART cycles in which an embryo or egg and sperm transfer occurred that resulted in a live P ● birth: This is the highest of these six measures of ART success  ercentage of ART cycles...

Ngày tải lên: 05/03/2014, 16:20

... popcouncil.org/pdfs/horizons/ fpandpmtctrprt.pdf.2004 N 2004 Ministry of Health, Rwanda Evaluation of access to and utilization of prevention of mother-to-child transmission (PMTCT) services in Rwanda Kigali7 TRAC, ... know about the method often not offer it to women who would benefit from it.67 Current supply of both male and female condoms is highly inadequate.68 In particular, the supply of female condoms, ... enhanced and social marketing of emergency contraception should be encouraged All women and couples living with HIV or at risk of HIV infection should know about and have access to the means of dual...

Ngày tải lên: 05/03/2014, 17:20

... endogeneity of supply yield insignificant estimates of the impact of GP supply on health After using instrumental variable methods, they find a positive and significant association between GP supply and ... measures of physical and mental health, housing and socioeconomics status (SES) of the mothers of the ALSPAC children to construct a measure of the health and SES of the younger female population of ... examine the whether the supply of GPs has an effect on selfassessed health of individuals in England The analysis is based individual level data from the Health Survey of England and contains around...

Ngày tải lên: 14/03/2014, 09:20

... HIV and AIDS HIV infection deaths reflect a number of factors, including underlying rates of HIV risk behaviors, prevention of HIV transmission, early detection and treatment of HIV disease, and ... have poor prognoses The rate of cancer cases diagnosed at advanced stages is a measure of the effectiveness of cancer screening efforts and of adherence to followup care after a positive screening ... analyses of other measures are not included, but additional measures and data can be identified in the NHQR and NHDR appendix tables Effectiveness of Health Care The NHQR and NHDR describe methods, ...

Ngày tải lên: 14/03/2014, 14:20

Ngày tải lên: 15/03/2014, 23:20

... computed and the energy is divided by the period in number of samples This method of computation of squared ~aln per sample avoids the incorrect computation of the gain due to arbitrary location of ... system, The system parameters were updated every 100 samples Synthesis consists of two steps: Generation of the excitation signal and synthesis of speech Separation of the synthesis procedure into ... a discussion of an analysts-synthesis system which is convenient to study various aspects of the speech signal such as the importance of d i f f e r e n t parameters of features and t h e i r...

Ngày tải lên: 17/03/2014, 19:21

... the role of power in customer-supplier relations, the economic survival of companies; and plain stupidity, including the occasional stupidity of customers, of burdensome procedures, and of quality ... certification programs, and "approved" specialized courses This world also consists of a multitude of arbitrary and, at times, irrational demands imposed on thousands of businesses and suppliers by an ... Quality Professionalism and the Ideology of Control 149 What Is Professionalism? 149 The Ideology of Quality 151 Influence of the Military and the Ideology of Quality Control 154 Influence of the...

Ngày tải lên: 17/03/2014, 20:21

... to study design and analysis and interpretation of data CYA contributed to acquisition of data and interpretation of data MB contributed to acquisition of data and interpretation of the data EHE ... associated with low values of total sperm count, sperm concentration, normal sperm morphology, and motile sperm Weight loss was associated with an increase in total sperm count and semen volume among ... acquisition of data, analysis and interpretation of data ASA contributed to study design, acquisition of data and interpretation of data JO contributed to analysis and interpretation of data JPB...

Ngày tải lên: 22/03/2014, 12:20