... contains reagents and enzymes for the specific amplification of a 86-bp region of the IE1 gene of HCMV For extraction of DNA, an aliquot of 400 μl EDTA-anticoagulated blood was collected and added with ... performed with degeneration at 93°C for and the initial 10 cycles of 93°C for 45 sec and 55°C for Page of 60 sec, and then 30 cycles of 93°C for 30 sec and 55°C for 45 sec In each run, the provided ... positive control, and four calibrations (4-7 log10 copies/ ml) for defining the standard curve, were also included Calculations of Ct, preparation of standard curve and quantification of each sample’s...
Ngày tải lên: 12/08/2014, 04:20
... fundamental human rights, in the dignity and worth of the human person, in the equal rights of men and women and of nations large and small, and to establish conditions under which justice and respect ... expansion and balanced growth of international trade, and to contribute thereby to the promotion and maintenance of high levels of employment and real income and to the development of the productive ... Middle Ages of the salvation of the Kingdom of God on earth ’ The ‘secular remnants of this Christian theology of history’ facilitated ideas of an equal and common development of humanity...
Ngày tải lên: 01/11/2013, 08:20
Tài liệu An International Comparison of Milk Supply Control Programs and Their Impacts pot
... growth in the EU and Canada and created an economic incentive for imports o Slow domestic and export growth has pushed Canadian and European processors to invest and expand in the US and other countries ... gains in the US and New Zealand have been the lowest In New Zealand, the continued use of low input farming limits some of the gains that have been made from formulating and feeding of concentrated ... removal of price and production controls In 2001, the New Zealand Dairy Board, New Zealand Dairy Group, and Kiwi Cooperatives merged to create a single entity, the cooperative Fonterra Fonterra handles,...
Ngày tải lên: 22/02/2014, 05:20
Báo cáo khoa học: Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategies pot
... sequence between 50 and 200–300 nucleotides in length with apparently viable 5¢ and 3¢ splice sites (5¢ss and 3¢ss) at either end] Presently, there is evidence that inclusion of many of these sequences ... prevalent in human genes than previously thought [37] Nonetheless, from a human disease point of view, many pseudoexon intronic sequences seem poised on the brink of becoming exons (Fig 1) and a comprehensive ... cocktail of 2¢-O-methyl phosphorothioate oligoribonucleotides against different regions of this pseudoexon abolished pseudoexon inclusion and partially restored production of normal mRNA and CFTR...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf
... at chromosome 3p, mutations of the VHL suppressor gene and loss of heterozygosity (LOH) of 9p, 14q, 17p and 10q have been reported in cRCC [4] Trisomy and 17 and loss of the Y-chromosome are frequent ... pRCC [4], and, despite the good prognosis of chRCC, loss of various chromosomes, mutations of p53, LOH at 10q23.3 and telomere shortening have been reported [6] RO consists of mixtures of cells ... according to the Heidelberg Classification of Renal Cell Tumours [2] Seven specimens of pRCC, seven of cRCC, six of chRCC, and six of RO, besides adjacent pieces of unaffected tissue, were used in this...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... detailed study of uptake of Yb3+ by human Tf (hTf) by UV-vis and NMR spectroscopy, and inductively coupled plasma atomic emission spectrometry (ICP-AES) The binding of Yb-bound Tf to human erythroleukemia ... performed with a cell density of % · 105 cells per mL Preparation of FeC-Tf and ferric-saturated Tf (Fe2-Tf) Monoferric- (selective loading of Fe3+ in the C-lobe of Tf) and diferric-Tf were prepared ... Binding of Yb3+ to apo-hTf ¼ ½Yb3þ total À ½Yb3 Æ Tf The thermodynamics of binding of Yb3+ to apoTf Binding of Yb3+ to apo-Tf was investigated by addition of aliquots of Yb3+ to a solution of apo-TF...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Comparison of starch branching enzyme I and II from potato ppt
... required to clarify this and whether the effect of phosphate is of relevance in vivo Branching of linear dextrins To obtain a more detailed comparison of the mode of action of SBE I and SBE II, the branching ... U S S I O N Comparison of SBE I and SBE II on the amylose and amylopectin substrates To compare the activity properties of potato SBE I and SBE II, commercially available amylose and amylopectin ... debranching of the products showed that both isoforms produced a small fraction of dp 6–7 chains and a larger fraction of chains < dp 11– 14, and in addition SBE I produced a population of dp 29–30...
Ngày tải lên: 31/03/2014, 23:20
báo cáo hóa học: " Comparison of knee motion on Earth and in space: an observational stud" potx
... position of the knee, rounded to the nearest degree, at each sampling point; 2) the amplitude and direction (flexion or extension) of all excursions of >3° (Figure 1B); and 3) the average velocity of ... Excursions of knee joint Figure shows typical histograms of the excursions of the knee for different days of continuous data collection on Earth and in space Onboard the ISS, 80.1 ± 7.4 % of all ... calibration period, the mean of the RMS and the standard deviation of the RMS were calculated The threshold value was then calculated to be the 95% confidence interval of the interval RMS This methodology...
Ngày tải lên: 19/06/2014, 10:20
báo cáo hóa học:" Comparison of migration behavior between single and dual lag screw implants for intertrochanteric fracture fixation" pptx
... plus 5° of valgus of the femoral shaft axis Exaggerated walking kinematics of the left limb was simulated by ± 75° rotation of the actuator, which resulted in a 45° arc of flexionextension and a ... dynamic screw and plate for intertrochanteric fractures of the femur J Orthop Trauma 2001, 15:394-400 Bess RJ, Jolly SA: Comparison of compression hip screw and gamma nail for treatment of peritrochanteric ... SH, Patel AD, Bircher M, Calvert PT: Fixation of intertrochanteric fractures of the femur: a randomised prospective comparison of the gamma nail and the dynamic hip screw J Bone Joint Surg Br...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo khoa học: " Comparison of rectal volume definition techniques and their influence on rectal toxicity in patients with prostate cancer treated with 3D conformal radiotherapy: a dose-volume analysis" ppt
... compared to methods and 2, relatively larger rectum volumes were contoured in methods and Table shows the comparison of rectum minimum, maximum and mean doses and percentage of rectal volumes receiving ... to the rectal volume (Dmax and Dmean, respectively), and volumes (percentage and absolute) of rectum receiving 30 Gy, 40 Gy, 50 Gy, 60 Gy, and 70 Gy For each patient and each technique, DVHs http://www.ro-journal.com/content/4/1/14 ... http://www.ro-journal.com/content/4/1/14 touring of the target volume and organs at risk; ET and MY collected the samples; AY gave advise on the work and helped in the interpretation of the data; EE and SS made the treatment...
Ngày tải lên: 09/08/2014, 09:22
báo cáo khoa học: "Microspatial differentiation of Drosophila melanogaster populations in and around a wine cellar in southern Spain Angeles ALONSO-MORAGA" pdf
... allele and sex, genotype and sex and genotype and mode of capture were studied by means of a loglinear model for multiway tables described by UrroN (1978) III Results Table shows the numbers of ... MuNoz-SERRANO A., 1986 Allozyme brium of Adh and a-Gpdh loci in wine cellar and field ter Experientia, 42, 1048-1050 polymorphism and linkage disequilipopulations of Drosophila melanogas- ARBANCHO B ... the Adh, a-Gpdh and Est-6 loci using the buffer system of P (1957) OULIK length individuals, using = Various standard statistical methods including t-test, Chi-square and analysis of variance, were...
Ngày tải lên: 09/08/2014, 22:22
Báo cáo y học: " Comprehensive comparison of three commercial human whole-exome capture platforms" pdf
... databases (release of 2010.12.01), Agilent targeted 4,871 with 86% of genes having >95% of CDs covered, in comparison with NimbleGen’s 4,642 genes with 83% of genes and >95% of CDs covered (Figure ... merged dataset of 21,326 genes from the CCDS (release of 2009.03.27), refGen (release of 2009.04.21) and EnsemblGen databases (release 54), and micro RNA genes using 719 genes from the human microRNA ... interrogate human disease genes using four known data sets (see below) Of the 5,231 unique genes collected from OMIM (release of 2011.03.10), HGMD (Professional 2009.2), GWAS (release of 2011.03.03) and...
Ngày tải lên: 09/08/2014, 23:20
báo cáo khoa học: "The effects of β-glucan on human immune and cancer cells" pptx
... the in vitro and in vivo animal biological studies of β-glucans, particularly on their immune and anti-cancer mechanisms Physical and chemical properties of β-glucan β-glucans are one of the most ... chromatography (HPLC)[17] and less often X-ray crystallography [18] or atomic force microscopy [19] However, due to the tedious and lack of quantitative nature of most of these technical methods, ... half-life, volume of distribution and clearance [27] The low systemic blood level of β-glucans after ingestion does not reflect the full picture of the pharmacodynamics of β-glucans and does not rule...
Ngày tải lên: 10/08/2014, 22:20
Báo cáo y học: " Comparison of ankle-brachial pressure index and pulse wave velocity as markers of cognitive function in a community-dwelling population" potx
... increased PWV and risk of cognitive impairment due to a number of factors including the measurement site of PWV, use of the MMSE, having a relatively small sample size and the possibility of a selection ... interpreted the data and wrote the initial draft of the manuscript SK had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data ... usefulness of ABI and PWV as markers of cognitive function in a community-dwelling population The measurement of the ABI provides one of the most practical means to objectively assess the presence of...
Ngày tải lên: 11/08/2014, 16:22
Báo cáo y học: " Functional and phenotypical comparison of myofibroblasts derived from biopsies and bronchoalveolar lavage in mild asthma and scleroderma" ppsx
... biopsies and BALF and was involved in organization of the manuscript GMV: One of the initiators of this study who has collaborated in the organization of the manuscript AS: One of the initiators of ... Expression of fibronectin isoforms in fibroblasts from BALF and bronchial biopsies from patients with mild asthma and SSc Expression of fibronectin isoforms in fibroblasts from BALF and bronchial ... organization of the manuscript, performed all two-dimensional 2DE experiments and sample preparation prior to the mass MALDI-TOF-TOF analysis, and performed some of the cell migration assays and cell...
Ngày tải lên: 12/08/2014, 16:20
Báo cáo khoa học: "A comparison of decision-making by physicians and administrators in healthcare settings" ppt
... of the fairness of the organization [5] This may be particularly relevant in situations where budget constraints are severe In a model of instructors and students, the use of the principles of ... little analysis of the current status of its principles or the impact of implementing them [8] In the example given, the principles of justice are relevant and applicable Appreciation and clear articulation ... ‘Directoring’ and managing in a professional system Modern Pathol 1998, 11:585-592 Jane Jacobs: Systems of Survival: A Dialogue on the Moral Foundations of Commerce and Politics New York: Random House;...
Ngày tải lên: 13/08/2014, 03:20
Báo cáo y học: "Comparison of cooling methods to induce and maintain normoand hypothermia in intensive care unit patients: a prospective intervention study" ppsx
... techniques during induction and maintaining of mild hypo- and normothermia in terms of efficiency and cooling performance Materials and methods Study population A total of 50 consecutive adult patients ... cooling consisted of rapid infusion of 30 ml/kg ideal bodyweight of lactated Ringer's solution at 4°C, followed by surface cooling using ice and/ or coldpacks The timing and amount of ice and coldpacks ... control module of the cooling device The primary endpoints of the study were the initial rate of temperature decrease, expressed as °C/h and the percentage of time the temperature was out of range...
Ngày tải lên: 13/08/2014, 08:20
Báo cáo y học: "Comparison of different methods to obtain and store liver biopsies for molecular and histological research" pot
... populations, and research of complex diseases profits from the fact that dogs share the human environment In addition to similarities between dogs and humans with respect to physiology, pathobiology, and ... genomic studies This kind of highthroughput gene expression profiling requires the use of high quality mRNA Likewise is the quality of mRNA of major impact on the reliability of the results in quantitative ... and wrote the first draft of this manuscript JIJ performed the IHC and co-wrote the first draft of this manuscript BB and BAS did the molecular analysis TSGAMvdI evaluated the histology LCP and...
Ngày tải lên: 13/08/2014, 13:20
Báo cáo y học: "CpG Immunotherapy in Chenopodium album sensitized mice: The comparison of IFN-gamma, IL-10 and IgE responses in intranasal and subcutaneous administrations" docx
... m ice Figure The comparison of cytokines and antibody levels The comparison of cytokines and antibody levels The mean values of systemic and local concentrations of cytokines and IgE antibody ... analyze the effect of I.N versus S.C administration of CpG in mucosal responses we measured the IL-10 and IFN-γ in BAL fluids of different groups of mice We found that IFN-γ and IL-10 were both ... effects of different administration route of CpG, we selected a number of immunological parameters such as IFN-γ, IL-10 and IgE Indeed, the measurement of immune responses regarding TH1 and regulatory...
Ngày tải lên: 13/08/2014, 13:22
Báo cáo y học: " Comparison of the McGrath® Series 5 and GlideScope® Ranger with the Macintosh laryngoscope by paramedics" pptx
... inventories Until now, the efficacy and potential advantages of the McGrath® Series and the GlideScope® Ranger in the hands of paramedics for the management of prehospital airways has not been ... conception, acquisition of data and drafting the paper KW was involved in conception and acquisition of data FH has made substantial contributions to analysis and interpretation of data CW has made ... 20 21 22 23 24 25 26 27 Page of Ochroch EA, Hollander JE, Kush S, Shofer FS, Levitan RM: Assessment of laryngeal view: percentage of glottic opening score vs Cormack and Lehane grading Can J Anaesth...
Ngày tải lên: 13/08/2014, 23:20