... 3.3.1.3 Comparison oftheeffectsofpHLandLeo on the activities of SOD, CAT, GPx and GST in the cortex following TBI 78 3.3.2 Comparingthe anti- apoptoticeffectsofpHLandLeo 79 3.3.2.1 ... 3.3.1 Comparingthe antioxidant effectsofpHLandLeo 75 3.3.1.1 DPPH radical-scavenging activities of pHL, Leoand VC (positive control) 75 3.3.1.2 EC50 values of VC, pHLandLeo ... cleaved PARP in the brain tissue to identify theeffectsofpHL on anti- apoptotic mechanisms 1.2 Studies to address theeffectsofLeo on antioxidant activity andthe expression ofapoptotic pathway...
... together with the degree of overhang ofthe prosthesis in millimeters andthe presence or absence of posterior osteophyte Each prosthesis was then scored radiographically by summing the degree of ... preparation and analysis ofthe results of this study I would like to acknowledge the help of Matthew Hankins ofthe Brighton and Sussex University Department of Statistics, for his advice and statistical ... between the varus/valgus positioning ofthe femoral component andthe Oxford score (0.38) Examples of good and poorly positioned prosthesis can be seen in Figures and Edmondson et al Journal of Orthopaedic...
... coli cells In the structure the base of bound GMP is stacked between Val143 and Phe194 N1, N2 and O6 ofthe nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH ofthe ribose is hydrogen bonded to the main chain andthe side chain of Asp142 (Fig 1C) Both hydroxyl groups ofthe ribose are involved in ... Asp142, 2¢ and 3¢OH ofthe ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate ofthe GMP is hydrogen bonded to main chain and side chain atoms of amino...
... of cysteines The involvement of four conserved cysteines in the coordination ofthe [4Fe–4S] cluster has been confirmed in WhiB3 [9] and WhiB4 [15] The brown color ofthe purified protein andthe ... conditions [15] The rate of disintegration was directly correlated with the duration and strength ofthe oxidizing environment Similarly, in this study, the intensity ofthe brown color andthe iron–sulfur ... except WhiB2, the amino acid immediately after the C-terminal cysteine ofthe CXXC motif is proline (Fig S6) In WhiB2 the absence of proline and therefore ofthe kink might not allow the protein...
... structure ofthe C2-SurE to a resolution of ˚ 2.7 A In both crystal forms the electron density accounted for the majority ofthe residues There were no disordered regions in the map The quality of refinement ... features ofthe protein St SurE is an aba sandwich protein and adopts the Rossmann fold as in archaic and thermophilic homologues The St SurE monomer consists of 13 b-strands, six a-helices and three ... is the jth observation of reflection h and is its mean intensity a on the other side (Fig 1A,B) One noticeable difference between St SurE andthe other SurEs is the short length of g1 and...
... of new therapies and ⁄ or vaccines, or the improvement of existing ones, necessary to control this disease The identification and characterization ofthe proteins involved in the interaction of ... (HABP) involved in the binding to and invasion of monocytes (U937) and type II alveolar epithelial cells (A549), using synthetic peptides The protein encoded by the Rv2560 gene was synthesized in 20-mer-long, ... did not predict the existence of a signal peptide, the high grand average of hydropathicity (GRAVY) value (0.488) andthe existence of four possible transmembrane helices in the complete Rv2560...
... established Of two possible dimeric structures of LPL, the head-tohead andthe head-to-tail models, the studies ofthe chimeric proteins of hepatic lipase and LPL andthe tandem repeat approach of LPL ... the C-terminal domain of apoCII interacts with the interface ofthe N- and C-terminal domains of LPL and part ofthe lid surface [32] Because the functional importance of LPL’s C-terminal domain ... illustrates the overall saddle shape ofthe dimeric structure andthe key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top ofthe hollow (Fig 1A) The...
... (Table 1) They display, however, greater heterogeneity in the molecular mass ofthe detected protein band, as is also the case for metal cation dependence, as the sPPase activity of many of these ... regulated by the same factors Analysis ofthe promoter regions of 3954 Sequence and phylogenetic analysis Knowledge ofthe N-terminal sequences ofthe three cyanobacterial sPPases purified and characterized ... salt (MgCl2) and mm Na4PPi (standard assay conditions) The reaction was started by the addition of enzyme, andthe PPi released after 10 was determined When the efficiencies of other divalent...
... follicles were cut open andthe theca and adherent granulosa cells peeled from the stroma The granulosa cells were then gently scraped from the theca andthe granulosa and theca cells were snap ... [21,22] The objectives ofthe studies reported here were to examine the interactions ofthe gonadotrophins and IGF with the Akt and Erk signalling pathways in theca and granulosa cells in vitro and ... Analysis of variance using the GLM procedures of SAS was used to determine theeffectsof treatment on the levels of Akt, p-Akt, Erk and p-Erk in granulosa and theca cells All values are given as the...
... with head and trunk turned to the opposite side The supraclavicular part ofthe plexus was exposed through a transverse incision cm above the clavicle at the root ofthe neck andthe C5 and C6 roots ... patients andthe final grading of their elbow and shoulder function are detailed in Table Table details the motor grade ofthe recovered muscles andthe final range of movement achieved Table shows the ... ofthe location in the ulnar nerve The chosen fascicle is separated from the rest ofthe nerve It must be divided cm distal to the level ofthe possible coaptation to turn laterally to meet the...
... both myopathy and neuropathy [4] The combination of neurophysiological testing with neuromuscular histology could therefore help in the precise identification ofthe type and severity of neuromuscular ... both syndromes Because of this, and because ofthe lack of studies evaluating neuromuscular disorders in ICUs using a combination of histopathological (nerve and muscle) and electromyographic ... tetraparesis and failure to wean Peripheral electromyographic analysis and neuromuscular biopsy were performed in all of these patients to determine the cause of limb weakness and diaphragm dysfunction The...
... very poor Furthermore, patients and relatives, regardless ofthe functional outcome, were asked whether they were satisfied with their treatment on our neurocritical care unit Materials and methods ... probability of early death) The possibility of answering the question of retrospective consent to neurocritical care was dichotomized Finally, (d) patients were asked whether they were satisfied with their ... http://ccforum.com/content/14/4/R144 Page of tests were two-sided, andthe significance level was set at a = 0.01 The distribution ofthe data was assessed with the Kolmogorov-Smirnov test Continuous and categoric variables...
... development, vision and other sensations The cerebral cortex, which makes up the superficial aspect ofthe cerebrum, is the most complex and important structure ofthe human brain The surface ofthe human ... as the largest and most prominent part ofthe human brain, is the control center of it It is 85% ofthe weight of a human brain Structurally, it is divided into two hemispheres, the left andthe ... coefficient measures the extent of inter-connectivity among the neighbors ofthe node It is defined as the ratio ofthe number of existing connections among the node i’s neighbors and all their possible...
... ascertain the exact position of each atom in the asymmetric unit ofthe crystal Calculation of electron density is dependent on two main factors; the amplitude andthe phase ofthe diffracted beam These ... products during each cycle ofthe PCR reaction It is one ofthe technologies ofthe genomic age and has become the method of choice for detection of gene expression at the mRNA level Several factors ... Wu Jinlu, Ms Tang Xuhua, Ms Sunita and, Mr Jobi andthe rest ofthe lab mates for the valuable discussion and friendship andthe present and former members of Functional Genomics Laboratory as...
... to the family Enterobacteriaceae Most ofthe E coli strains are harmless and are found in the intestines of mammals The harmless strains are part ofthe normal flora ofthe gut, and can help their ... for EPEC, except for the presence ofthe Stxencoding phage on the EHEC chromosome andthe presence ofthe characteristic EHEC 60-Mda plasmid instead ofthe EAF plasmid of EPEC The EHEC plasmid is ... (e.g., the immunoglobulin G proteases andthe VacA toxin) Examples of T5SS encoded by PAI are LPA andthe EspC PAI ofthe pathogenic E coli, SPI-3 of S enterica, and SHI-1 of S flexneri (Schmidt and...
... assess the global burden of disease It is calculated as the sum of years of life lost and years of life lived with disability The overall incidence rate of stroke is around 2-2.25 per thousand population ... to the stroke patients Study Period: Clinical data set was collected at the point of admission ofthe patients to the two community hospitals and upon their discharges from the hospitals The ... ofthe medical complication/s (2) Date of medical complication/s The information on the following variables was recorded at the point of discharge (planned -discharges) ofthe patients from the...
... interspersed by a stimulus which deviated from the standard in the intensity ofthe first component andthe glide direction ofthe second constituent The number of distinct MMN components elicited by ... et al The relationship of age, gender, handedness, and sidedness to the size ofthe corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness ... P300 andthe N2b Data indicate that the P300 is involved not only in processes of working memory, but may interact with the N2b in the control of motor response to external cues Through the use of...
... included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full) A younger member ofthe household present at the time ofthe interview ... assessment ofthe informant ofthe health status ofthe elderly person was obtained Results The non-response rate for the study was only 1.7% In response to the question "Are you healthy ?", 49% of males ... in the urban areas and from the Grama Niladhari divisions (smallest administrative units) in the rural areas Using the electoral register for each ofthe clusters, 20 persons of age 60 years and...
... independently ofthe presence of AREs (Fig 6B, lanes and 5) It is possible that in the region located before the AREs, consisting of 343 bp ofthe 3¢ UTR ofthe IL-1b transcript, there is a putative ... contained 343 bp ofthe 3¢ UTR region (nucleotides 898–1227 ofthe 3¢ UTR region) The construct encoding IL-1b cDNA with AREs (IL1+ARE) contained 423 bp ofthe 3¢ UTR (nucleotides 898–1318 ofthe 3¢ UTR ... mRNA and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP andthe level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA...