commercial utilization of knowledge from in vivo protein biosynthesis

... organizational learning: Representing and maintaining knowledge in an experience base, in Proc Tenth Int Conf on Software Engineering and Knowledge Engineering, SEKE98, 1998 44 T Dingsứyr, A lifecycle ... reasoning for reusing software knowledge, in Proc Second Int Conf ICCBR97, 1997 40 A Birk and C Tautz, Knowledge management of software engineering lessons learned, in Proc 10th Int Conf on Software ... article examines the literature on knowledge management initiatives in the software engineering domain We examine case studies reported from dierent organisations, to examine: (1) What kind of knowledge...

Ngày tải lên: 16/01/2014, 16:33

... Roles of prostaglandin D2 in adipogenesis in vivo Y Fujitani et al indicate that overexpression of H-PGDS increases insulin sensitivity in vivo Adipocyte differentiation ex vivo Finally, we examined ... pancreatic islets to increase insulin secretion Indeed, serum insulin levels were increased after treatment with thiazolidinediones in diabetic mice through regulation of insulin production in pancreatic ... in WAT of TG mice than in WAT of WT mice Serum levels of triglyceride, glucose, leptin and insulin, and insulin sensitivity, in TG mice After weeks of normal or HF diet, serum levels of triglyceride,...

Ngày tải lên: 16/02/2014, 09:20

... Figure Interaction of Domain and General Knowledge '59 i sented by the nodes Martin et al [12] define a transportable NL interface as one that can acquire a new domain model by interacting with ... domain concepts are indirectly linked to the semantic procedores that are used to interpret them Conclusions The general approach we have taken to defining the inter-component interactions in ... component along with the usual linguistic constituents in the interpretation of each phrase During parsing, semantic information is collected in "semantic" registers, which are inaccessible (by convention)...

Ngày tải lên: 17/03/2014, 19:21

... translocation of toxin peptides in cell-free endosomes containing in vivo internalized DT The EN was isolated 15 after the administration of native DT and then suspended in 0.15 M KCl containing mM ... endosomes into the external milieu Results In vivo endocytosis of DT and nontoxic mutant DT (mDT) within the endo-lysosomal apparatus of rat liver The kinetics of in vivo uptake of native DT or mDT into ... directed against DT at pH was inhibited by hexa-d-arginine (HA), a competitive inhibitor of furin, and the metal-chelating B Fig Effect of protease inhibitors on the proteolysis of native DT...

Ngày tải lên: 23/03/2014, 07:20

... of a-aminoadipic-d-semialdehyde in rat plasma protein Rat plasma protein from STZ-induced diabetic and control subjects was investigated for the presence of a-aminoadipicd-semialdehyde Protein ... copper-containing amine oxidase, catalyzes the oxidative deamination of the e-amino group of lysine residue of elastin and collagen, which are connective tissue proteins, to form a-aminoadipic-d-semialdehyde ... reflect changes in protein conformation as a result of the continuously decreasing loss of positive charge, and then the protein will be inactivated Lysyl oxidase, a copper-containing amine oxidase,...

Ngày tải lên: 31/03/2014, 08:20

... biology, in view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA ... Switzerland 2Department of Immunology, Page of National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan Laboratory of Experimental Hematology, Bordet Institute, University of Brussels (ULB) ... similar role in CD8+ T cells remains an interesting issue to investigate, as it might shed new light on the relationship between cytokine signaling and lymphocytes differentiation In contrast...

Ngày tải lên: 18/06/2014, 19:20

... biology, in view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA ... Switzerland 2Department of Immunology, Page of National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan Laboratory of Experimental Hematology, Bordet Institute, University of Brussels (ULB) ... similar role in CD8+ T cells remains an interesting issue to investigate, as it might shed new light on the relationship between cytokine signaling and lymphocytes differentiation In contrast...

Ngày tải lên: 20/06/2014, 03:20

... assistance Digital image is obtained by primary digitizing straight from a digital camera or by secondary digitizing – scanning of the aerial image The information obtained in this way is called a record ... remote sensing researches mostly the qualitative aspects of features (e.g damage to the forest stands) At the beginning of the 90’s, forestry mapping changed from the analogue making of the maps ... not bring the expected results although the attained accuracy of point position was quite good However, other methods how to achieve the goal were searched, including the testing of digital interpretation...

Ngày tải lên: 07/08/2014, 03:22

... or 5% bovine serum albumin (BSA) in TBST Incubation with primary antibody anti-PTBP1 (Polypyri­ midine tract-binding protein 1; Proteintech Group Inc (Chicago, Illinois, USA) at 1:1,000 in 5% skimmed ... following antagomir treatment (Figure S4C in Additional file 1), confirming the suitability of antibodies against them for in vivo profiling In contrast, analysis of CDC42 and PTBP1 proteins in ... examining insulin resistance in skeletal muscle paint a remarkably similar picture - one of no striking change in protein coding mRNA abundance In the Patti et al study [2], muscle samples from...

Ngày tải lên: 11/08/2014, 12:20

... if more intensive rehabilitation is required In England there has been a rapid rise in the independent sector provision of in- patient residential and nursing care These are often far from a person’s ... Service, Royal Edinburgh Hospital, Edinburgh, UK 2Intensive Psychiatric Care Unit, Royal Edinburgh Hospital, Edinburgh, UK 3Division of Psychiatry, University of Edinburgh, Edinburgh, UK Authors’ ... In Edinburgh, most of those transferred from forensic services come from forensic rehabilitation wards in a medium secure unit They are transferred to psychiatric Admission during first 10y of...

Ngày tải lên: 11/08/2014, 15:22

... obtained from the breeding facility of the Institute of Poultry Sciences in Sichuan Agricultural University, China Animals were bred and maintained in an accredited facility at the Institute of ... Fifty goslings were randomly divided into groups In brief, a group of 40 goslings were orally infected with GPV CHV strain, using 0.1 mL of 103 LD50 per gosling Another group of 10 goslings was ... strain, a high-virulence strain of GPV, was obtained from Key Laboratory of Animal Diseases and Human Health of Sichuan Province Aleutian disease virus (ADV), canine parvovirus (CPV), porcine...

Ngày tải lên: 12/08/2014, 04:20

... Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, Maryland, 21205, USA Department of Emergency Medicine, Johns Hopkins School of Medicine, ... Other possible determinants of health service utilization are the direct and indirect costs of using the service and perceived quality of care [11] However, the latter determinants are context ... closer by UNHCR often also invests in local health services in refugee hosting areas (e.g., referral hospitals) which could help promote local access to them instead of services inside settlements...

Ngày tải lên: 13/08/2014, 15:21

... studies involves the use of isofemale lines, i.e the progeny derived from a single female inseminated in nature (Parsons and Hosgood, 1967; David, 1979; Wallis et al., 1985) The isofemale line method ... of individuals belonging to the same group (Falconer, 1981), i.e in the present work, individuals belonging to isofemale lines Hoffman and Parsons (1988) call this parameter (when using = isofemale ... and parasite strains of different geographic origins (Carton, 1984) Studies of the genetic variability among and within isofemale lines of successive generations of L bonlardi indicate that traits...

Ngày tải lên: 14/08/2014, 20:20

... affecting 3D scanning in- vivo We have also demonstrated the clinical applicability of the methodology in monitoring the rate of tooth wear progression in patients 4 Table of contents 1.#Introduction# ... of TMJ clicking and severe bruxism This comes in agreement with the findings of another study examining the association between para-functions (diurnal clenching and/or grinding and nail-biting) ... analysis of tooth wear in- vivo using 3D scanning technology Khaled Ahmed BDS, FPros, MSc RestDent, FHEA Submitted in fulfilment of the requirements of the Degree of Doctor of Philosophy School of Dentistry...

Ngày tải lên: 22/12/2014, 18:14

... protein- protein Chapter interactions in vivo but it requires the proteins to be expressed in yeast which is not the native environment for the protein of interest (unless it is a yeast protein) ... processes in a cell, protein- protein interactions play an important role in a cell system As a cell functions through a network of protein- protein interactions, it is vital to study these interactions ... expression of proteins in cells Due to its diffraction limited resolution of ~250 nm, it is not possible to detect protein- protein interactions even if two proteins are localized in the same pixel of...

Ngày tải lên: 10/09/2015, 08:38

... found in subcutaneous fat of young mice of both genotypes contained UCP1 The intensity of immunohistochemical staining of brown fat cells was stronger in transgenic than in control mice, in agreement ... emergence of brown adipocytes in white fat depots of mice, indicating involvement of at least four different genes [9] In contrast with the inability of UCP1 to induce multilocular cells in white ... also indicated a higher content of transgenic UCP1 in unilocular adipocytes in subcutaneous than in epididymal fat and suggest that UCP1 is not capable of inducing conversion of a unilocular into...

Ngày tải lên: 31/03/2014, 15:20

... Purification and refolding of N-terminal His-tagged gAd-GLP-1-A fusion protein The fusion protein was purified by High-Affinity Ni-IDA Resin After filtering, the fusion protein was bound in the column ... that majority of fusion protein was present in inclusion body (Fig 2B) Figure Maps of N-terminal His-tagged gAd-GLP-1-A fusion protein and gAd-GLP-1-A fusion protein (A) N-terminal His-tagged ... connect the N-terminus of gAd and the C-terminus of GLP-1-A Because the protein produced from plasmid vector PET28a was an N-terminal 6×His-tagged protein, we introduced an enterokinase cleavage...

Ngày tải lên: 25/10/2012, 11:10

... unfolded proteins with the heat-shock protein GroEL Nature 336, 254–257 25 Lorimer, G.H (1996) A quantitative assessment of the role of the chaperonin proteins in protein folding in vivo FASEB ... identified by other means as an in vivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that aggregate at 42 °C in E coli containing a dnaK deletion mutation (DnaK ... UP12 is a stress protein DISCUSSION In this work, we have identified two proteins GatY and UP12 as putative in vivo substrates of the chaperonin GroEL In addition to its in vivo interaction with...

Ngày tải lên: 22/02/2014, 07:20

... follows: Mainstreaming ARH within the Ministry of Health Mainstreaming within the Ministries of Education, Sports, and Youth Integrating lessons learned among partners and organizations working with ... Senegalese Ministry of Sport integrated ARH issues into its pre-service training curriculum for schools, and into its masters degree in Sports Education A training of trainers was held for the ministerial ... attended this gathering Program officers from the attending countries expressed much interest in having their own Ministries of Health play the leading role in adolescent programming in collaboration...

Ngày tải lên: 05/03/2014, 17:20

... checkpoint signaling in vivo It may function in the sensing of DNA damage [22] In contrast with the intensive study of RPA in animals and yeast, little is known about this protein in plants In 1997, ... transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein was purified ... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound...

Ngày tải lên: 07/03/2014, 21:20