combined raman spectroscopy and confocal microscopy

... was used to study the morphology and chemical composition of the samples The transmission electron microscopy (TEM) and the high-resolution transmission electron microscopy (HRTEM) images were ... using a Renishaw (RMRe1000) micro -Raman spectrometer at room temperature Raman scattering modes were excited by means of the 514 nm line of an Ar+ laser, and the Raman signals were measured in a ... formed The Raman spectra of bulk single-crystal silicon (c-Si) and SiNWs are shown in Fig A Raman peak at 520.2 cmÀ1 with the full-width at half-maximum (FWHM) of 4.6 cmÀ1 can be seen in the Raman...

Ngày tải lên: 16/03/2014, 15:19

... tape,42,43 gasoline,58 hair dye,46 soil,49 pen ink,51 and tire rubber.59 19 CHAPTER RAMAN SPECTROSCOPY 2.1 Review of Raman Spectroscopy Raman spectroscopy is a vibrational spectroscopic technique ... Discriminant Analysis 15 CHAPTER RAMAN SPECTROSCOPY 19 2.1 Review of Raman Spectroscopy 19 2.2 Blue Toners 21 2.2.1 Materials and Methods 21 2.2.1.1 Instumental ... called the Raman effect This shift provides information about vibrational, rotational and other low frequency transitions in molecules Raman spectroscopy can be used to study solid, liquid and gaseous...

Ngày tải lên: 24/08/2014, 11:52

... and guidance and my committee members: Dr Melissa Kacena and Dr Robert Yost I would also like to thank Dr Kacena and her lab for their help with the osteoclast cell culture, Dr Keith Condon and ... mice have two loxp sequences and produces a 520bp band STAT3+/+ mice lack the loxp sequences and therefore produce a smaller490bp band Cre mice produce a 615bp band if it is present (Figure 7) ... chromosome and transcription of CTSK is activated by RANKL and M-CSF Transforming growth factor β1 (TGFβ1) and interleukin 10 (IL-10) both inhibit CTSK, which has a molecular mass of 24 kDa and consists...

Ngày tải lên: 24/08/2014, 12:40

... IR and Raman spectroscopy in various scientific disciplines ranging from chemistry and physics to bioscience and medicine On the other hand, handling, manipulating and evaluating of IR and Raman ... at ~L ˆ 9394 cm–1 and the bands at wavenumbers lower and higher than n 9394 cm–1 arise from Stokes and anti-Stokes Raman scattering, respectively On the other hand, a standard Raman spectrum comprises ... version provides four different workspaces The workspaces NIR.ows and RAMAN. ows are particularly devoted to NIR spectroscopy and Raman spectroscopy, respectively All functions of OPUS are available...

Ngày tải lên: 17/03/2014, 14:46

... the book focuses on the analysis and An Introduction to Confocal Imaging presentation of confocal images The field of confocal microscopy is now extremely large, and it would be impossible to include ... of confocal, two-photon, and four-dimensional imaging Lists meetings and workshops, and a booking form for reserving time on the instruments in Madison 6.1.2 Confocal Microsxope Companies www .microscopy. bio-rad.com ... courses and CSH Press publications msa .microscopy. com Web site of the Microscopy Society of America contains useful links to other sites and microscopy societies Details of their annual conference and...

Ngày tải lên: 11/04/2014, 01:50

... ultraviolet and visible wavelength ranges (0.3–2.5 lm) Raman spectroscopy was also used to study these samples using a Jobin-Y von T64000 Raman spectrometer The observations from the FTIR and Raman spectroscopy ... 4b) Raman Spectroscopy The Raman spectroscopy data of the samples were first analysed using the Tuinstra–Koenig (TK) equation The background to this equation, which is important for Raman spectroscopy ... absorption band, at 900 cm-1, is an absorption band that can be assigned to the stretching vibration of Si–OH or Si–O- groups A broad absorption band, situated between 3,000 and 3,600 cm-1, and another...

Ngày tải lên: 22/06/2014, 01:20

... IR and Raman spectroscopy in various scientific disciplines ranging from chemistry and physics to bioscience and medicine On the other hand, handling, manipulating and evaluating of IR and Raman ... at ~L ˆ 9394 cm–1 and the bands at wavenumbers lower and higher than n 9394 cm–1 arise from Stokes and anti-Stokes Raman scattering, respectively On the other hand, a standard Raman spectrum comprises ... version provides four different workspaces The workspaces NIR.ows and RAMAN. ows are particularly devoted to NIR spectroscopy and Raman spectroscopy, respectively All functions of OPUS are available...

Ngày tải lên: 29/06/2014, 06:20

... Overview of Raman spectroscopy 1.3.1 The Raman effect 1.3.2 Raman instrumentation 1.3.2.1 Confocal Raman microscopy 1.3.2.2 Fiber-optic Raman spectroscopy ... vivo confocal Raman diagnostic modality was superior to NIR autofluorescence (AF) spectroscopy, but marginally higher than the co-contributions of NIR AF and confocal Raman spectroscopy The confocal ... 0.88, 0.86 and 0.56 for the confocal Raman, the composite NIR AF /Raman, and NIR AF spectroscopy modalities, respectively 80 Figure 5.1 (a) Comparison of mean in vivo Raman spectra ± standard...

Ngày tải lên: 10/09/2015, 09:24

... that CARS microscopy allowed chemically specific and label-free imaging in living organism Le et al combined CARS microscopy with TPEF microscopy and Raman spectroscopy to visualize and analyze ... appreciation and respect to Professor Dietmar W Hutmacher and Professor Hanry Yu and their group members, who taught me useful knowledge on biology and biomedicine research and offered me cellular and ... emission for easy filtering and detection In the previous work of M-CARS spectroscopy, a narrow band picosecond dye laser and a broadband pulsed dye laser were used as the pump and Stoke beam, respectively...

Ngày tải lên: 11/09/2015, 10:00

... light scattering spectroscopy, fluorescence spectroscopy, and Raman spectroscopy have been comprehensively investigated for cancer and precancer diagnosis and evaluation [1-24] Raman spectroscopy ... conformation and structures, and circumvent the strong autofluorescence signals with the use of VIS or near-UV excited Raman       spectroscopy, deep UV (>300nm) and NIR excited Raman spectroscopy, ... utility of random forests with NIR Raman spectroscopy for precancer diagnosis       Specifically, Chapter provides the overview of Raman technique and its development for precancer and cancer...

Ngày tải lên: 16/10/2015, 15:37

... SPFM and SPFS techniques on biosensor development was not evaluated This study focuses on the development of surface plasmon enhanced fluorescence spectroscopy (SPFS) and microscopy (SPFM) and ... spatial vector and t is the time The relations between D and E, B and H are given by D(r, t ) = εε E(r, t ) B(r, t ) = µµ H (r, t ) (2.2) with ε and µ being the dielectric constant and the magnetic ... help and instructions of many colleagues and friends, and it is my pleasure to acknowledge their contribution I’d like to give my greatest respects to my two supervisors, Prof Wolfgang Knoll and...

Ngày tải lên: 08/11/2015, 17:01

... Energy and Environment (IJEE), Volume 3, Issue 5, 2012, pp.715-730 717 Figure T-s diagram for the combined cycle Exergy analysis and optimization The following assumptions are made for simplicity and ... T H, Anderson A, Agnew B A hybrid gas turbine cycle (Brayton/Ericsson): an alternative to conventional combined gas and steam turbine power plant Proc Instn Mech Engrs., Part A, J Power and Energy, ... and optimization for combined Brayton and inverse Brayton cycles Int J Ambient Energy, 2007, 28(1): 15-26 [8] Alabdoadaim M A, Agnew B, Alaktiwi A Examination of the performance envelope of combined...

Ngày tải lên: 05/09/2013, 16:10

... of medaka embryos and the granules in HeLa cells FRAP revealed that it moved with two diffusion components in both PGCs and HeLa cells: 0.15 and 0.01 lm2ặs)1 in PGCs, and 0.9 and 0.03 lm2ặs)1 ... time point t, T0 and Tt represent the uorescence in the whole cell at time points and t, respectively, and B0 and Bt represent the uorescence in the bleached region at time points and t Diffusion ... and to measure the mobility of the components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and...

Ngày tải lên: 18/02/2014, 16:20

... Ozaki and Slobodan Sasic 1.1 1.2 1.3 History of Raman Spectroscopy The Principle of Raman Spectroscopy An Example of Simple Raman Spectrum: Raman Spectrum of Water 1.4 Characteristics of Raman Spectroscopy ... APPLICATIONS OF RAMAN SPECTROSCOPY Raman and Krishnan [Raman 1928] discovered the scattering effect that is named after Raman and that earned him a Nobel Prize in 1930 Raman spectroscopy, however, ... Raman, most notably ‘‘The Handbook of Vibrational Spectroscopy, ’’ by P Griffiths and J Chalmers, and ‘‘Analytical Applications of Raman Spectroscopy by M Pelletier However, the literature on Raman...

Ngày tải lên: 08/03/2014, 19:20

... number and position, as well as an estimation of the bandwidth and the intensity of the bands making up the amide I or I¢ region Thereafter, curve fitting was performed and the heights, widths and ... of the IR amide I band, located between 1700 and 1600 cm)1 and arising mainly from the C ¼ stretching vibration of the peptidic bond This band is conformationally sensitive and can be used to ... Structure and thermal denaturation of crystalline and noncrystalline cytochrome oxidase as studied by infrared spectroscopy Biochemistry 33, 11650–11655 Krimm S & Bandekar J (1986) Vibrational spectroscopy...

Ngày tải lên: 16/03/2014, 13:20

... sequencing In both its E- and Z-configurations, peptide was characterized further by NMR spectroscopy peptide charges because bands with nearly the same charge (bands 1B, 1C and 2) migrated quite ... the intact a-PEC and the derived peptides The excellent solubility of peptides and at pH 7.0 recommended their employment for further studies such as crystallization and NMR spectroscopy Unfortunately, ... Hildebrandt, P (1998) Fourier transform near-infrared resonance raman spectroscopic study of the a-subunit of phycoerythrocyanin and phycocyanin from the cyanobacterium Mastigocladus laminosus J Raman...

Ngày tải lên: 17/03/2014, 10:20

... characteristics and the native cultural and historical values As for the typical bird watching tourists, they all aim at observing the typical features of birds and recognize bird species and their ... and shapes of canals, beaks, tongues that there is such a great variety of birds’ twittering and songs Moreover, some bird species can even imitate other tones and sounds through their life and ... ensured that avifauna be typical and attractive, infrastructure be adequate and involved staff be technically trained Bird watching ecotourism is typical and potential and has received attention to...

Ngày tải lên: 28/03/2014, 15:20

... Near-infrared FT -Raman spectroscopy 1170 (R4) Resonance Raman spectroscopy all-trans spirilloxanthin in LH1 The Raman bands from 15-cis spirilloxanthin in the RC normally seen at 1528, 1239 and 1160 ... wavelengths of both the 915 and 889 nm forms shift to blue, and the emission bands get broader (Fig 2) For the 915 nm form, the peak wavelength shifts from 945.4 to 939.5 nm, and the bandwidth increases ... when the a- and b-polypeptides of LH1 have higher flexibility [28] Raman lines R5 or R6 overlapped with the intense carotenoid band (m3) and therefore cannot be resolved For both the 915 and 889 nm...

Ngày tải lên: 30/03/2014, 02:20

... the implementation was a combined partial parser and tagger that would be reasonably fast, but at the same time easy to modify and maintain At the time of designing and implementing the parser, ... Bonhomme, and Laurent Romary 2000 XCES: An XML-based standard for linguistic corpora In Proceedings of the Linguistic Resources and Evaluation Conference, pages 825–830, Athens, Greece Daniel Janus and ... phase, the parser loads the external tagset specification and the ruleset, and converts the latter to a set of compiled regular expressions and actions Then input files are parsed one by one (for...

Ngày tải lên: 31/03/2014, 01:20

... Fung, and C Y Lee, Pflug Arch 436, 371 (1998) p p Lui, S K Kong, D Tsang, and C Y Lee, Pflug Arch 435, 357 (1998) 22 THEORY AND PRACTICAL CONSIDERATIONS [21 and flaws in the use of confocal microscopy ... presenting confocal images, and confocal microscopes all offer stereoscopic views of confocal data sets without requiring additional software Stereo pairs are generally produced in confocal microscopy ... record sequentially and are only random access on playback There are both erasable and nonerasable random access devices; in the past this meant also a distinction between magnetic and optical media,...

Ngày tải lên: 11/04/2014, 09:38