coli o157 h7 in sausages

... C-terminal 43 residues (amino acid positions at 31–59 and 148–190) of EspA corresponding to the binding interface of CesA could be clearly solved The other regions corresponding to the amino acid ... 7), according to Scheme For ANS binding (Fig 3), the ANS transition midpoint can be considered to be the apparent NI to NII transition midpoint, assuming that the maximum ANS intensity in Fig corresponds ... for input data The urea-induced unfolding curves were obtained by plotting the ellipticity at 222 nm against urea concentration To estimate the urea concentration of midpoint of the unfolding...

Ngày tải lên: 30/03/2014, 16:20

... 26-98 strain of E coli O157: H7 incubated in the presence of the MIC of rifampicin followed by the MBC of gentamicin E coli O157: H7 was grown for 18 hrs at 37°C in rifampcin-containing TSB followed ... Group First Injection (Hour 0) Second Injection (Hour 1) Third Injection (Hour 17) E coli O157: H7 (3 × LD50) Rifampicin (MIC) - E coli O157: H7 (3 × LD50) Gentamicin (MBC) - E coli O157: H7 (3 × LD50) ... administered in vivo]/E coli O157: H7 CFU per in vitro MIC reaction Consequently, the ratio of rifampicin to E coli O157: H7 CFU determined by in vitro MIC testing was maintained in vivo Similarly,...

Ngày tải lên: 12/08/2014, 17:20

... The 282 amino acids from the carboxy terminus of Intimin have been reported to be involved in binding to its receptor Tir [23,24] The region of Tir involved in the interaction with intimin has also ... EspA, Intimin, and Tir domains For eliciting an immune response against E coli O157: H7, studies have shown that production of the carboxy terminal part of Intimin in a transgenic plant cell line ... Subunit vaccines based on intimin and Efa-1 polypeptides induce humoral immunity in cattle but not protect against intestinal colonisation by enterohaemorrhagic Escherichia coli O157: H7 or O26:H...

Ngày tải lên: 13/08/2014, 16:20

... kháng nguyên O157 H7 KIT E coli O157: H7 Latex Test Kit hãng Remel Các khuẩn lạc vi khuẩn E coli O157: H7 cho phản ứng dương tính với KIT Các chủng vi khuẩn xác định E coli O157: H7 phản ứng miễn ... thấy rằng: chủng E .coli O157: H7 phân lập từ thịt bò mang gen mã hóa độc tố Stx eae thử nghiệm khả gây bệnh tích tế bào VERO chứng minh chủng E .coli O157: H7 ký hiệu M61 có khả sinh độc tố độc tố ... phút Lượng protein đưa vào giếng 17 g III KẾT QUẢ VÀ THẢO LUẬN 3.1 Kết xác định gen mã hóa độc tố chủng vi khuẩn E .coli O157: H7 phân lập đƣợc Bốn chủng vi khuẩn E .coli O157: H7 phân lập từ 143...

Ngày tải lên: 26/02/2014, 02:20

... hiểm cho người O157: H7 Bệnh E .coli O157: H7 phát lần vụ ngộ độc thực phẩm ăn bánh hamburger chế biến từ thịt bò Hoa Kỳ vào năm 1982 (Riley LW cs, 1983) Cho đến bệnh E coli O157: H7 gây phổ biến ... pathogenicity for humans and stx genotype in Shiga toxin-producing Escherichia coli serotype O157 Eur J Clin Microbiol Infect Dis 27: 227-232 Leung PH, Peiris JS, Ng WW, Robins-Browne RM, Bettelheim KA, ... xác định số độc tố Shiga 34 chủng vi khuẩn E coli O157: H7 có khả sản sinh độc tố Shiga phân lập số tỉnh Nam Trung Bộ Trong số 34 chủng E .coli O157: H7 có 27 chủng (79,41%) mang gen độc tố Stx1...

Ngày tải lên: 10/03/2014, 23:20

... Escherichia coli (VTEC) O157 in Swedish dairy herds Epidemiol Infect, 133: 349-358 Griffin PM Escherichia coli O157: H7 and other enterohemorrhagic Escherichia coli In: Blaser MJ, Smith PD, Favdin JI, ... Escherichia coli O157: H7 infection in humans (1995) Annal of Internal Medicine 123:698-714 11 Vu-Khac H and Nancy NA (2008) Prevalence and genetic profiles of Shiga toxinproducing Escherichia coli strains ... E coli O157: H7 từ mẫu phân trâu, bò khỏe mạnh số tỉnh Nam Trung Bộ Xác định tỷ lệ nhiễm vi khuẩn E coli O157: H7 trâu, bò khỏe mạnh - Xác định số đặc điểm sinh vật, hoá học chủng E coli O157: H7...

Ngày tải lên: 20/03/2014, 01:21

... E coli outside the host intestine [21] We screened highly curliated EHEC O157: H7 strain 4304 showing routine curliation even at higher growth temperature 37oC in LB broth culture (Fig 2A) In ... were defective in curliation including that reflected by the host intestine of cattle and/or humans In this regard, we hypothesized that curliation in EHEC O157: H7 may play a role in adherence ... GA, Keen JE, Elder RO Variations in the csgD promoter of Escherichia coli O157: H7 associated with increased virulence in mice and increased invasion of HEp-2 cells Infect Immun 2002, 70, 395-399...

Ngày tải lên: 07/08/2014, 17:22

... of E coli O157: H7 in the clinical laboratory is dependent on distinguishing the pathogenic serotypes from normal fecal flora containing commensal strains of E coli Fortunately, E coli O157: H7 has ... against H7 antiserum, they all possessed H7 genes (Table 5) RAPD fingerprinting analysis Eleven E coli O157: H7 isolates were compared with the 17 E coli O157: H7 strains which were obtained from ... strains were positive In agglutination test against O157 antiserum, all strains showed positive, but one of them did not agglutinate against H7 antiserum Characterization of E coli O157: H7 isolates...

Ngày tải lên: 07/08/2014, 18:21

... in gnobiotic piglets have shown that an E coli O157: H7 intimin-negative mutant expressing the EPEC intimin adheres to different bowel sites, suggesting that the EPEC and E coli O157: H7 intimins ... of Escherichia coli O157: H7 and other Shiga toxin-producing E coli strains In: Kaper JB, O'Brien AD (eds.) Escherichia coli O157: H7 and Other Shiga Toxin-Producing E coli Strains pp 195-209, ... E coli O157: H7 have also been reported in Canada, Japan, and the United Kingdom [35] However, accumulating data indicate that non -O157 EHEC infections may be more frequent than E coli O157: H7...

Ngày tải lên: 07/08/2014, 20:23

... độc lực Haemolysin (Hly) Intimin: gen eae (E coli attaching-effacing) Serine protease (EspP) hệ thống tiết loại II Shiga-like toxin (Stx): Stx1 Stx2 Stx1 khác Stx2 nu = acid amin Stx2: Stx2c, ... CÁC NHÓM E coli GÂY BỆNH EPEC (enteropathogenic E coli) ETEC (enterotoxigenic E coli) EIEC (enteroinvasive E coli) EHEC (enterohaemorrhagic E coli) EAggEC (enteroaggregative E coli) UPEC (uropathogenic ... CNTY - ĐHNL Tp.HC Xét nghiệm nhanh vi khuẩn E coli O157: H7 •* Lọc 100ml nước có gây nhiễm E coli O157: H7 •* Rửa lọc 3ml môi trường BHI •* Tăng sinh 37OC •* Chuẩn bò DNA mẫu (10 phút) •* Chạy...

Ngày tải lên: 11/08/2014, 03:23

... protein bands Since Felix O1 is LPS-specific [15], we analyzed wV8resistant mutants of E coli O157: H7 An overnight broth culture of an E coli O157: H7 strain was mixed with excess wV8 and incubated ... Control of Escherichia coli O157: H7 infection of calves by bacteriophages 4th International International Symposium and Workshop on Shiga toxin (verocytotoxin)-producing Escherichia coli (VTEC 2000) ... bacteriophage wV8 Bacteria E coli O157: H7 Other E coli Sensitive to bacteriophage wV8 Resistant to bacteriophage wV8 Reference strains of 12 common E coli O157: H7 phage types1 (1 strain/phage type) The...

Ngày tải lên: 12/08/2014, 04:21

... Emerg Infect Dis 2006, 12:780-786 Whittam TS: Evolution of Escherichia coli O157: H7 and other Shiga toxin-producing E coli strains In O157 Escherichia coli: H7 and Other Shiga Toxin-producing E coli ... genotypes in STEC O157 strains of human and cattle origin Frequencies Frequencies of 42 polymorphism-derived genotypes in STEC O157 strains of human and cattle origin O157 strains genotyped in this ... frequency in STEC O157 strains of human and bovine origin (Figure 2), indicating that STEC O157 of this genetic background may have an advantage in populating cattle and/or causing disease in humans...

Ngày tải lên: 14/08/2014, 21:20

... involved in the regulation via interactions with the different proteins operating in the cascade, resulting in an integration of the various signals Indeed, UTase appears to monitor the glutamine ... a cassette containing these elements This cassette was constructed by inserting the following four DNA fragments (in Table Escherichia coli K-12 strains used in this study Strain Genotype References ... those in Fig Error bars indicate the standard error of the mean The line results from a linear regression calculation of the data points only because of a progressing change in the signals impinging...

Ngày tải lên: 23/03/2014, 04:21

... encoding pineapple stem bromelain was initially cloned into pENTR/TEV/D‐TOPO before being sub‐cloned into the expression vector pDEST17 (Invitrogen, USA) The expression vector containing recombinant ... of papain family, stem bromelain (E.C.3.4.22.32) contains 212 amino acid residues including seven cysteines, one of which is involved in catalysis (Bitange et al., 2008) Pure stem bromelain is ... carried out in triplicates Cell concentration (induction time) Induction serves as a turning point between cell growth and recombinant protein synthesis in E coli (Xu et al 2006) The cloning and...

Ngày tải lên: 21/06/2014, 17:20

... preventing coliform mastitis include hygiene measures and in some countries prophylactic immunization Incidence and severity of clinical signs of coliform mastitis were reduced using Escherichia coli ... SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA) Results Clinical findings After both challenges all cows became infected and developed clinical mastitis within 12 hours after inoculation One cow ... change, and clinical effects following intramammary Escherichia coli challenge for J5 vaccinate and control cows Clin Vaccine Immunol 2007, 14:693-699 Henderson B, Wilson M: Cytokine induction by...

Ngày tải lên: 12/08/2014, 18:22

... following IMPACT-CN system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against trypsin,and proteinases ... Standard molecular markers 2, Uninduced 1, 3, 5, Induced (containing 58kD fusion protein band) Cells were broken by sonication in 10mM Tris-HCl buffer pH8 containing 1mM EDTA (TE buffer) After ... target protein was released from the chitin column when the chitin-bound intein tag undergoes self-cleavage in the presence of DTT Fig 12 showed chitin gel before (N1) and after (N2) releasing MCoTI-II...

Ngày tải lên: 12/02/2014, 10:20