coli and the bacteriophage

... phage, and together with YMS contributed to the proteome analysis HWA and DMM carried out the electron microscopy and AV annotated the genome AM and EJL carried out the host range studies TW and ... relationship The authors declare that they have no competing interests RA originally isolated phage V8 AMK assisted in the annotation and prepared the manuscript, EJL and SO propagated and purified the ... and resistance of bacterial cultures to bacteriophage wV8 Bacteria E coli O157:H7 Other E coli Sensitive to bacteriophage wV8 Resistant to bacteriophage wV8 Reference strains of 12 common E coli...

Ngày tải lên: 12/08/2014, 04:21

... coupled to the absorption or release of protons by the protein or the ligand If this is the case, the binding enthalpy is dependent on the ionization enthalpy of the buffer in which the reaction ... mM MgCl2, 100 mM NaCl The upper panels show the raw data for the heat effect during the titrations; the lower panels are the binding isotherms model The results indicated the existence of a minor ... NarG(1–28)] and solved their structures by NMR; NarG(1–15) corresponding to the predicted N-terminal helix and NarG(1–28), which included both the N-terminal helix and the b-sheet present in the mature...

Ngày tải lên: 16/02/2014, 14:20

... The reaction mixtures were as in the standard assay, except that the components specified in the Results were added, and the concentrations of the substrate and enzyme varied as required for the ... [19,20] The results of the fluorescence titration experiments are summarized in Fig In the absence of MgCl2, the affinity of Fpg for the THF:C ligand was 1.6-fold higher than for the THF:A ligand The ... on the binding of Fpg to the THF:C ligand and slightly improved binding to the THF:A ligand, making it comparable with binding to THF:C (Fig 2B, groups and 2) Therefore, it is unlikely that the...

Ngày tải lên: 18/02/2014, 18:20

... affinity of the subunits for the substrate (KS1 and KS2) and for Pi (KI1 and KI2), presented in Table and Table 2, respectively, are independent of the Zn2+ ion concentration In order to support the ... representation of the catalytic cycle for APase from E coli A model describing the catalytic mechanism of APase from E coli, based on the results of the kinetic experiments and in accordance with the data ... constants KI1 and KI2, describing the affinity for Pi, differ less than constants KS1 and KS2 Therefore, the dimer with the substrate bound to the high affinity subunit (21) is more stable than the dimer...

Ngày tải lên: 21/02/2014, 00:20

... accompany the redox change Specifically, there is a switch in the heme axial ligand and changes in the flexibility of the FG loop (residues 86–96) in the protein when the redox state of the heme ... the polarity of the heme distal side, the direction of the heme-bound O2 molecule, and the redox potential of the heme iron [5] The reason why the auto-oxidation rate of the Leu99 and Leu115 mutants ... proximal side and ⁄ or the bond length between the heme iron and the proximal ligand may contribute to the formation of the 5-coordinated NO–heme complex We therefore speculate that the bond strength...

Ngày tải lên: 16/03/2014, 13:20

... class diagram shows the existence of classes and their relationships in the logical view of a system UML modeling elements in class diagrams - Classes and their structure and behavior - Association, ... provide to the actor when the use cases is executed Typical contents - How the use case starts and ends Normal flow of events Alternate flow of events Exceptional flow of events 10 Uses and Extends ... and its subclasses There are two ways to find inheritance: - Generalization - Specialization Common attributes, operations, and/ or relationships are shown at the highest applicable level in the...

Ngày tải lên: 22/08/2012, 10:37

... to the toiling and dying types who made it over to America And once in America, people divided once again: you could say they became the poor and the rich The losers and winners The artists and ... narrative structure, and to the kind of stories that get told and retold about the Irish, so as to reveal both the regularity of English colonial discourse on Ireland and the Irish and the mutations ... take up and extend Said’s insight, in Culture and Imperialism, that the ‘‘convergence between the patterns of narrative authority constitutive of the novel on the one hand, and, on the other, a...

Ngày tải lên: 21/09/2012, 10:39

... on these estates The other contingent lacked the unity of the Richmond tenants These were Bretons from the north-east of the duchy who received grants of land in the midlands, the south-west and ... ± The barons of Leon and Penthievre could construct forti®cations without the permission of the duke;  Á ± The barons of Leon and Penthievre had the right of wreck on the shores of their lands; ... Blain and the à forest of Le Gavre.24 South of the Loire, ducal domains included the castellany of Le Pallet,25 estates on the south bank of the Loire and another in the extreme south-west of the...

Ngày tải lên: 21/09/2012, 10:46

... Night and the following day, they were ‘‘everyone’’ and ‘‘London’’ and even ‘‘England.’’ They were created as a group by the newspapers, and this chapter examines the mechanism of their creation and ... the food stocks, and the mood of the garrison The tactics of the Daily Mail captured the attention of the nation; the newspaper dramatized the situation of the town by emphasizing the danger that ... don’t know they know the public’’ (quoted in Koss Rise and Fall ) The Victorian cult of personality had moved into the press by the turn of the century, and the military version of the focus...

Ngày tải lên: 21/09/2012, 10:54

... the Netherlands, Luxembourg, Switzerland, north-east Spain and northern and papal Italy – are clearly within the Western and European purview.7 Ancient Greek philosophy, Jewish spirituality and ... on the mountain There is the hint in the Rembrandt that the seventeenth-century interpretation of Moses had him invested with more personal agency in the carriage of the laws; neither God nor the ... the rise of the state 6.1 The birth of the state 6.2 Legal diversity and universality in the emerging European states 6.3 The decline of the Christian commonwealth 6.4 The arrival of the state...

Ngày tải lên: 21/09/2012, 10:54

... Sinbad the Sailor Poe and the printed word and Jack and the Beanstalk; new London editions of these two works appeared the year the Allan family came to England.3 So did new editions of The History ... the Rotunda: ``They have nearly ®nished the Rotunda ± The pillars of the Portico are completed and it greatly improves the appearance of the whole ± The books are removed into the library ± and ... from the form of their original publication, and these removals affect interpretation The appearance of the printed page, however, shapes the reader's understanding of the text it contains ``The...

Ngày tải lên: 21/09/2012, 10:58

... variety of approaches They examine the structure of the press; the methods used to control it by political authorities and their effectiveness; the journalistic texts themselves; and the political role ... texts, their presentation and format, the topics they covered, the way issues were presented and the messages, overt and implicit, that they contained Such a comprehensive approach to the comparative ... political press was the product of the struggles for independence from Britain and to forge a new nation, and in the Netherlands, the outcome of the Patriot Revolution of the s In France...

Ngày tải lên: 21/09/2012, 10:58

... conservative propaganda, and with the break up of the radical associations and the consequent erosion of the links between these organisations and other intellectual currents and circles, the conditions ... of the age The complex intersection of these two histories – the political turmoil of the 1790s and the broader hegemonic shift towards the meritocratic bias of the professional classes – demands ... Wordsworth’s observation gathers together fears about the French Revolution, the Industrial Revolution, and 10 The Crisis of Literature in the 1790s the information revolution ( the rapid communication...

Ngày tải lên: 21/09/2012, 11:00

... taken, the straight between the points either all fall on the same side of the surface, or some on the same side, and some on itself, but none on the other side /5/ And, when ... the center of the sphere, I call the figure internally contained by the surface of the cone, and by the surface of the sphere inside the cone, a “solid sector.” /6/ And when two cones having the ... direction and either one of them is wholly contained by the other and by the straight having the same limits as itself, or some is contained, and some it has common; and the contained...

Ngày tải lên: 21/09/2012, 11:00

... individual, hand-to-hand combat Grand strategy, on the other hand, remained the preserve of Tiw.11 In India, too, much the same phenomenon was apparent in the role of Indra, the chief of the Vedic ... negative, then the process was repeated, and a second mission sent This time, the Aztec ambassadors anointed the right arm and head of the other ruler, set a tuft of feathers on his head and gave ... formal demands to the other side and then to inform the other side straight away that Rome was making war against it in the event of their rejection.58 The obsolescence of the fetial procedure...

Ngày tải lên: 21/09/2012, 11:02

... grow quickly and others not That is, the variation in the dependent variables comes from both the time series and the cross-sections, and some pooling of data is necessary to address the questions ... and liberalization, on the one hand, and growth and growth volatility, on the other, were tradeoffs in democracies We explore briefly both lines of inquiry as a guide to further research 13 The ... IMF’s, is linked to the date of the liberalization Eichengreen forthcoming reviews the data and methodological differences among most of these and other studies (For discussions of the different data...

Ngày tải lên: 24/10/2012, 08:50

... widening of the exchange rate band and then abandonment of the band altogether.31 Indeed, the dating of 29 The methodology and use is described in Fraga, Goldfajn and Minella (2003) See also Central ... measured by the standard deviation of the variable 21 Are the findings being driven by the way the sample is partitioned and by the choice of comparator countries? The robustness of the results ... the upper panel and the locus of points showing the variability in the exchange rate and inflation is plotted in the lower panel Points to the south and west in Figure are welfare superior, and...

Ngày tải lên: 24/10/2012, 09:27

... along the circuit, the financial system governs the supply and demand of funds and, at the end of the process, it determines the condition for the closure of the circuit As discussed, the conditions ... to the endtargets and the timeframe They should explain to the public the reasons for agreeing on lower requirements and that they hold institutions to the announced standards This would put the ... of the circuit round and under what conditions They therefore stand to benefit significantly from factoring the long-term market potential of borrowing firms in their risk analysis The higher their...

Ngày tải lên: 24/10/2012, 09:33

... zero even at the 95% level in Canada, Finland, the Netherlands, Norway, Sweden, Switzerland, the UK and the US Moreover, while in some countries, (including Finland, the UK and the US) residential ... Canada, Denmark, Finland, France, Germany, Ireland, Italy, Japan, the Netherlands, Norway, Spain, Sweden, Switzerland, the UK and the US The sample starts in 1986 in order to avoid the more turbulent, ... the whole sample period are available for Australia, Canada, Switzerland, Denmark, Finland, France, the Netherlands, Sweden, the UK and the US For, Belgium we link an older series for small and...

Ngày tải lên: 24/10/2012, 09:40

... the transaction standards and the quality of business activities for enhancing the efficiency of export and import activities, expanding the external economic relations and co-operate with other ... in the Mekong Delta linking to Cambodia The total length of the inland waterway (IW) system is about 40,998 km, mainly in the Red River Delta and the Mekong Delta In the past few years, inland ... forwarding industry to open the door of development now and in the future How they use those keys properly to open the door? It depends on other factors, which include troubles and their own capability...

Ngày tải lên: 27/10/2012, 16:42