cocaine apos s effects on gonadotropins and gonadal steroid hormones

... addition to a direct effect on osteoclasts and osteoblasts, some authors suggest that a bisphosphonate induced obliteration of the regional blood vessels could lead to an avascular osteonecrosis ... alteration of collagen gene expression (figure 2) Discussion Bisphosphonates are therapeutically applied to treat metabolic bone diseases, such as osteoporosis or metastasis to the bone Clinical studies ... osteoblasts cyclin D1 gene expression or type I collagen gene expression These results support the assumption, that for the inhibition of farnesyl pyrophosphate (FPP) synthase enzyme [6,7] non-nitrogen-containing...

Ngày tải lên: 11/08/2014, 20:20

... microarray and to determine the specific location of Sox9 expression, immunohistochemistry was performed on E13.5 embryos Because Sox9 is known to be expressed in chondrocytes, expression in Meckel s and ... This mouse model had limited usefulness because Mmu16 has many genes that were not present on Hsa21 and is also missing several important genes found on human chromosome 21 It was also impossible ... or bone formations develop, but it is also present in other areas such as the central nervous and urogenital systems (BI 1999; WRIGHT 1995) Sox9 expression is necessary for the differentiation...

Ngày tải lên: 24/08/2014, 12:33

... used as markers for cell synchronization Coomassie stain for histone was used as loading control previous studies, we found that H3Ser28 phosphorylation was only observed during mitosis, indicating ... cells In contrast with our observations, they reported that MLL1 remains associated with condensed chromatins even during mitosis, but is degraded at late M (mitosis) and S phases To address 10 ... chromatin condensation, decondensation and differential expression of cell cycle-associated proteins are critical for the correct progression and maintenance of the cell cycle As MLLs and H3K4-specific...

Ngày tải lên: 23/03/2014, 06:20

... were given as mean ± SD and statistical analysis was carried out by t-test P values less than 0.05 were considered as statistically significant Results Effects of siRNA on DNMT1 mRNA and protein ... effective strategy in cervical treatment Conclusions In conclusion, our study demonstrates that DNMT1 silencing could suppress proliferation and induce apoptosis of Hela and Siha cells DNMT1-siRNA ... hypermethylation of tumor suppressor genes Rhee et al Figure Effects of DNMT1 silencing on gene methylation and mRNA expression of seven tumor suppressor genes in Siha cells assayed by MeDIP...

Ngày tải lên: 10/08/2014, 10:21

... Authors’ contributions ACMY performed RT-PCR assays, flow-cytometry assays and IMTC participated in virus culture and virus isolation WYL was responsible for experimental design, analyses and drafting ... 12:1203-1207 de Jong MD: H5N1 transmission and disease: observations from the frontlines Pediatr Infect Dis J 2008, 27 :S5 4 -S5 6 Homme PJ, Easterday BC, Anderson DP: Avian influenza virus infections II Experimental ... cultures was reversely transcripted to cDNA using the poly(dT) primers and Superscript III reverse transcriptase (Invitrogen), and quantified by real-time PCR The sense and antisense primers used...

Ngày tải lên: 12/08/2014, 02:20

... luciferase activity by the Luciferase Assay System (Promega, USA) Statistical analysis Data were presented as mean ± standard deviation (SD) The differences between groups were assessed with student s ... Since T lymphocyte proliferation is primarily mediated by IL-2, inhibition of IL-2 production is a central mechanism of action of several immunosuppressants such as CsA This study also demonstrated ... student s t test and corrected with the Bonferroni test Correlations between AC concentration and activity parameters were calculated with Pearson productmoment correlation test P < 0.05 was considered...

Ngày tải lên: 13/08/2014, 14:20

... expression system (teton/tet-off system) Tissue-specific expression elements either in front or downstream of the promoter must restrict the expression of the site of interest Furthermore, insulator ... inevitable This prolonged treatment per se carries a certain health risk because TNF is essential to normal immune response and tumour suppression An increased risk for infection (tuberculosis) and motor ... division, resulting in short-term transgene expression A major limitation is the acquired immune response against adenovirus serotype in most humans, and seven out of 10 samples of RA synovial...

Ngày tải lên: 09/08/2014, 01:24

... change in expression was then obtained as (2-ΔΔC T) and the graph was plotted using log 2-ΔΔCT Statistics Statistical evaluations were done by ANOVA, expressed as mean ± S. E.M using In Stat (Ver.2.04a) ... receptor and CREB in STZ-induced diabetes proves its potential in managing CNS disorders in diabetes Phospholipase C mediates transduction of neurotransmitter signals across membranes via hydrolysis ... [13,14] Also curcumin modulates the expression of various molecular targets, such as transcription factors, enzymes, cytokines, cell cycle proteins, receptors and adhesion molecules [15] Diabetes mellitus...

Ngày tải lên: 10/08/2014, 05:21

... significance of differences was assessed by one-way ANOVA followed by Bonferroni-Dunnett s test Results were represented as mean S. E.M Differences were considered significant for p < 0.05 Results ... groups Data represent means ± S. E.M Five rats were used in each group *; p

Ngày tải lên: 07/08/2014, 18:20

... inflammatory diseases, such as Socs3 in T cells from the colon mucosa of patients with Crohn 's disease [31] and over-expression of SOCS1, SOCS2 and SOCS3 in the epidermis of psoriatic skin lesions, as well ... in pSS research Studies of apoptosis in SGs from SS patients have demonstrated increased expression of the apoptosis inducing Fas and Fas ligand genes [10], whereas the degree of apoptosis was ... B10.Q.Nss1/Idd5 The analysis was focused mainly on differentially expressed genes from three comparisons: B10.Q versus B10.Q.Nss1, B10.Q versus B10.Q.Nss1/Idd5, and B10.Q.Nss1 versus B10.Q.Nss1/Idd5...

Ngày tải lên: 09/08/2014, 10:21

... expression in normal and degenerate NP cells as well as the effect of IL-1β and TNF-α on cytokine gene expression and the expression of the associated receptors and endogenous antagonists There ... Boos N: Expression and distribution of tumor necrosis factor alpha in human lumbar intervertebral discs: a study in surgical specimen and autopsy controls Spine 2005, 30:44-53 discussion 54 Seguin ... motif 4) and ADAM-TS5, resulting in a net catabolic response Previous studies from this laboratory have investigated the expression of IL-1β and associated receptors in disc degeneration and shown...

Ngày tải lên: 09/08/2014, 14:20

... spleen and liver mRNA expression Future examination of macrophage responses at other tissue sites and responses of other cell types is clearly warranted Parthenolide 's in vitro effects on mediators ... was modestly suppressed which contrasts with many in vitro studies suggesting anti-inflammatory effects of this compound It is possible that the differences in metabolism and/ or distribution ... mechanisms by which parthenolide imparts its effects on inflammatory responses Investigations of the anti-inflammatory properties of parthenolide, and feverfew have focused on suppression of primary...

Ngày tải lên: 11/08/2014, 08:21

... GMA-MS and GMA-SS exposure Reported in tables 1, 2, and are the associated categories of diseases and disorders, molecular and cellular functions and physiological system development and functions ... These gene interactions were also present in the B6 response to GMA-MS welding fume, which suggests similar transcriptional regulation to both MS and SS fumes in this strain at weeks (Figures ... susceptible A/J strain, and GMA-SS fume exposure was associated with chronic overexpression of inflammatory genes This transcriptional response supports our previous finding that GMA-SS is more potent...

Ngày tải lên: 12/08/2014, 11:22

... Fusion assay between ASCT2-transduced and syncytin-Ory1-transduced co-cultured cells demonstrates that ASCT2 is the syncyFigure tin-Ory1 receptor Fusion assay between ASCT2-transduced and syncytin-Ory1-transduced ... strongly suggests that Env-Ory1 uses the ASCT2 receptor, as does human syncy- In situ hybridization of placenta sections To further assess the physiological relevance of syncytinOry1 expression in ... placenta-specific expression, are fusogenic in ex vivo cell-cell fusion assays, and one of them displays immunosuppressive activity [6] A pair of env genes from endogenous retroviruses (ERVs) were...

Ngày tải lên: 12/08/2014, 23:22

... DNA content) does not mean that the cells are synchronized Synchronized divisions are the sine qua non of synchrony Criteria for successful analysis of gene expression during the division cycle ... false positives and false negatives should be considered in the analysis Just because a particular gene result fits pre-existing data collected by classical means, one must not consider this to ... G1-phase DNA contents Criteria for cell synchronization and analysis of gene expression 8) The size distribution of newly synchronized cells should be narrower than the size distribution of the original...

Ngày tải lên: 13/08/2014, 23:20

... (163) Metabolism 120 -S 141 +S 62 -S 19 -S 34 +S 20 Protein synthesis 55 -S 81 +S 13 -S -S -S 32 +S Energy 21 -S 32 29 +S +S, sub-model over-represented; -S, sub-model under-represented See text for ... analysis is related to genes controlled by carbon and nitrogen interactions, information from this study can also be used to globally identify genes and processes responsive to regulation by carbon ... analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1,000 rapidly responding genes and new linkages to glucose, trehalose-6-phosphate, iron, and sulfate metabolism Plant...

Ngày tải lên: 14/08/2014, 14:21

... genes specifically overexpressed in HIV/SIVassociated and/ or non-HIV/SIV-associated DLBCLs, we compared transcription levels of the selected genes in HIV/SIV-associated and human non-HIV-associated ... ND4, SMG-1 was increased in most HIV- and non-HIVassociated lymphomas These genes may be involved in both HIV-associated and non-HIV-associated lymphomagenesis Some genes of this group (set, SMG-1, ... transcription of mitochondrial genes can be associated with the early stages of oncogenesis [34] The SMG-1 kinase gene was upregulated in one human HIV-associated, one monkey SIV-associated and several...

Ngày tải lên: 02/11/2012, 11:08

... successful staining of the test samples, clear negative controls and noise, and an expected expression of housekeeping genes The samples were very homogeneous (figure 2) The expression study was ... function is necessary, as well as of biochemical processes and of the biophysical properties provoked amongst others by a moderate repression of the gene expression Gene locus 19p13 on the short ... technology and quantitative RT-PCR, we assessed the transcriptional responses resulting from the incubation of HeLa cells with the transporter molecules [36] Material and Methods Peptide Synthesis and...

Ngày tải lên: 03/11/2012, 11:44

... Basel, Switzerland) and details will be published elsewhere Plasmids and siRNAs Construction of expression vectors for HA-tagged fulllength mouse p46shc, p52shc, and p66shc and the introduction ... progression [10] However, the intracellular processes subsequent to disruption of cell–cell adhesion remain elusive Tumor metastasis is a multistep process that, in addition to the loss of cell–cell ... demonstrated that siRNA-mediated knockdown of all Shc isoforms strongly reduced Decma-induced Erk phosphorylation Moreover, expression of silent mutants of Shc isoforms showed that only p46Shc and...

Ngày tải lên: 19/02/2014, 05:20