coagulase negative staphylococci cns s aureus and mrsa with the pvl gene
Cloning genes by PCR
Ngày tải lên :
25/10/2013, 22:20
... recommended In some cases six-base enzymes give little success due to the rare distribution of such sites, in which case a fourbase recognition site enzyme, such as Sau3A, should be used For the first-round ... complementary ends that are capable of annealing The consequence is a process called suppression, which results in such self-associated molecules being unavailable as templates for PCR This suppression phenomenon ... coding sequences using the genetic code Most amino acids are encoded by more than one codon In some cases it is possible to select the most likely codon (s) based on the bias in codon usage displayed...
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Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
Ngày tải lên :
16/03/2014, 05:20
... assay buffer SDs, indicated by error bars, are based on at least two measurements The results reported above showed that the lactate dehydrogenase activities present in the parental strain and ... interactions with other residues, as we will discuss below It is clear that the titration curve of the catalytic histidine in LDH is more extended than the corresponding one in LDHB Discussion L lactis ... modeling Given the considerable similarity between the amino acid sequences of LDH and LDHB and lactate dehydrogenases with known structures, the derivation of their structures on the basis of available...
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Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot
Ngày tải lên :
17/03/2014, 21:20
... “NOSPECS” classification Grades a, b and c within class I, class II, class III and class IV are largely undefined Severity should be scored by skillful experts in GO The classification score should ... autoimmune diseases The first section of this book is focused on genes, gene expression and signaling pathways involved in autoim‐ mune pathogenesis The second one attempts to present current ... problems in future research The pathogenesis of Graves’ disease As described above, GD is considered a Th2 autoimmune disease Generating TRAb is the essential for development of the disease, as TRAb...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
Ngày tải lên :
07/08/2014, 18:21
... distinguish strains with different virulence factors from different sources, we could not define the difference in the genetic patterns between strains possessing only stx1 or stx2 and strains ... somatic and flagellar antigens (O157, H7) These steps are necessary since other enteric bacteria can be sorbitol -negative and can possess antigens those are identical to or cross-reactive with O157 ... advantages of time and cost-saving, sensitivity, and no special skills required to perform The results of RAPD patterns in this study compared with the study of Radu et al [36] They reported clusters...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
Ngày tải lên :
07/08/2014, 18:21
... that the sequence of E chaffeensis EC-PGHL clustered closely on the same branch with the USA isolates These observations suggest the possibility that E chaffenesis may have migrated between USA and ... (U23503) strains, all of these originate from the USA (Table 2) The phylogenetic analysis also revealed that the E chaffeensis EC-PGHL formed a single cluster with the above strains (Fig 2) Discussion ... DNA and has been used successfully in a number of studies However, this technique is labor intensive and requires that the results of multiple reactions be analyzed for each sample In this study,...
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Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
Ngày tải lên :
09/08/2014, 10:23
... involved in SAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome, which is an oligoarthritis associated with acnes and pustilosis [37,38] – was detected in ReA and UA samples Detection ... to the synovium in such patients [6] Most Shigella ReA cases are caused by S flexneri [35], but sporadic cases associated with S sonnei and S dysenteriae have been described [4,35] The most recent ... Halomonas spp., Rhodococcus fascians and manganese-oxidizing bacteria (Table 4) These organisms have an environmental source (soil, plant and water) Other 1 6S rRNA gene sequences showing less than...
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Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
Ngày tải lên :
11/08/2014, 03:20
... assistance Authors’ contributions AOO performed the experiments and drafted the manuscript FS and JRW supervised the reporter assays MLS supervised the ChIP and EMSA experiments SML, FS, and MLS participated ... Pharmaceuticals, Raleigh, NC DMSO was used as the solvent for TMP in all experiments The maximum DMSO concentration was 0.1% in all assays This concentration of DMSO was tested in all assays and did ... MN), Assay Designs (Ann Arbor, MI) or eBioscience (San Diego, CA) RAW 264.7 cells were stimulated with μg/ml of LPS for 24 hrs and supernatants were collected for ELISA assays In each case, sample...
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Báo cáo khoa học: " Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant" potx
Ngày tải lên :
12/08/2014, 18:22
... interests Authors' contributions EB and SSL designed the study and EB performed the practical analyses and writing the manuscript, with some assistance from SSL KEJ was responsible for the PCR and ... for samples consisting of manure, soil, silage and biogas substrate before and after pasteurisation and digested residues were 200 colony forming units (cfu)/g Discussion Sequence analysis of the ... g and they were collected in clean pots The samples were stored at -20°C until further analysis Muscle samples collected by swabs Specimens were taken from most of the muscle tissues (22) by swabs...
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Báo cáo y học: "Wound healing and inflammation genes revealed by array analysis of ''''macrophageless'''' PU.1 null mice" ppt
Ngày tải lên :
14/08/2014, 14:21
... genes upregulated in fibroblasts in response to serum exposure Cluster analysis of these results hints at the roles of hundreds of genes by the similarity of their temporal profile with genes ... JNK signaling [47] For these reasons it is clear that our screen underestimates the numbers of genes associated with each of the repair episodes and is perhaps somewhat biased towards genes expressed ... for several other classes of genes expressed at low copy number A similar observation was made in a recent serial analysis of gene expression (SAGE) study of Drosophila genes expressed downstream...
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Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR
Ngày tải lên :
07/10/2015, 09:55
... amorphous material (*) or by fibrous tissue (+) x250 The adipose tissue infiltrating the muscle is made of cells of various sizes, often in association with mast cells (arrows) 19 These signs, reminiscent ... to the immune system, inducing a specific immune response Immunization with DNA thus mimics live infection, with the antigen synthesized endogenously by host cells This synthesis leads to the ... tissue samples were limited by sample, genetic and technique heterogeneity Therefore, it is the aim of this study, to investigate the spectrum of T-lymphocyte cytokines expressed in tissues (spleen,...
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ESTROGENIC ACTIVITY LEVEL OF NAKDONG RIVER BASIN AND ITS CONTROL BY WATER TREATMENT PROCESSES
Ngày tải lên :
05/09/2013, 08:40
... Estrogenic Activity and Concentration] Yeast Two-Hybrid Assay was used to evaluate the estrogenic activity This bioassay detects real estrogens (such as 17β-estradiol, estrone, and estriol) as ... interacts with transcription factors and other transcriptional components to modulate gene transcription This causes expression of the reporter gene Lac-Z, and the enzyme produced (β-galactosidase) ... well as all known xenoestrogens (such as alkylphenol-ethoxylates and ethinylestradiol) Upon binding an active ligand, the occupied receptor then binds to the estrogen-response sequences (EREs) and...
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Moment and Shear, Generated by DL on the Composite Section
Ngày tải lên :
29/09/2013, 12:20
... Modulus of elasticity for the strands Ep := 28500 Number of strands at middle Ns_middle := ye4 Eci = 4695.982 Ns_middle = 34 sp 2 ceil Area of pre-stress strands Aps_1 := Ns_middle ⋅ Strand_area ... Total Initial Loses ∆fi := R1 + ES ∆fi = 21.895 Total Final Loses ∆ft := ES + SR + CR + R2 ∆ft = 52.085 Total Initial stress in the strands with Initial Losses fi := 0.75⋅ Strand_strength − ∆fi ... stresses are of opposite sign to that of the live load STI1 STI2 STI3 STI4 := STI1 STI2 STI3 STI4 STI5 DC LOADS (non-comp) DW Loads LL + I STI6 LOCATION self wt other...
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a discourse analysis of opening and closing speeches by masters of ceremonyon reality television showsin american english versus vietnames
Ngày tải lên :
26/11/2013, 12:41
... features and cohesive devices in AOSs versus VOSs and ACSs versus VCSs The results marked the significant influence of culture on the discourse features of both Vietnamese and American languages They ... Contents in ACSs and VCSs 93 Table 4.18 92 The Use of Adjectives Expressing Extreme Meaning in ACSs and VCSs Table 4.19 Phrase Types in ACSs and VCSs 100 Table 4.20 Sentence Types in ACSs and VCSs ... the different characteristics of ACSs and VCSs The same specific tasks done with AOSs and VOSs were conducted with ACSs and VCSs Finally, a conclusion was drawn for both opening and closing speeches...
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A RESEARCH ON ENGLISH INVERSION AND DIFFICULTIES ENCOUNTERED BY ENGLISH MAJORS OF HAI PHONG PRIVATE UNIVERSITY
Ngày tải lên :
11/12/2013, 23:48
... can see the inversion structure is stressed much more than the word there standing after the verb The former there is also distinguished from unstressed existential there which can also appear ... surprise In this case, so is followed by Subject plus Operator [120] a She is studying at University, and so is my sister Cô học đại học chị gái b She is studying at University, and so your sister ... In question operator is put before the subject [9] Will she ask any question? Is she asked any question? Is she asking any question? Has she asked any question? Has she been asking any question?...
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A study on imperative and errors made by vietnamese learners
Ngày tải lên :
11/12/2013, 23:51
... to question tags used in imperatives, occupies 78% Other question tags also causes Vietnamese learners make mistakes Such as: shall we? / can you? Let s and let us: Let s is used as the contraction ... some errors made by Vietnamese learners and some suggested solutions to overcome the errors Part III – the Conclusion which gives the reviews of the study and suggestion for further study PART ... contrast with the corresponding negative, which occurs earlier in the discourse Bolinger claims that such a contrast is always implicit in the use of do, but that in other cases the contrast is provide...
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A study on subjuntive and errors made by vietnamese learners
Ngày tải lên :
11/12/2013, 23:53
... If the action expressed in the object clause is simultaneous with that of the principal clause, the past subjunctive of the verb to be is used; with other verbs the same meaning is expressed ... paper These suggested solutions and corrections may not be exact, so I recommend the best version 49 CHAPTER IV: SOME SUGGESTED SOLUTIONS TO SOLVE THE ERRORS When the Vietnamese learners study and ... mood The subjunctive mood shows that the action or state expressed by the verb is presented as doubts, possibility, wishes, desire or anything else contrary to fact The subjunctive mood has synthetic...
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Tài liệu How to Win Friends and Influence People by Dale Carnegie docx
Ngày tải lên :
24/01/2014, 00:20
... listeners to go out and test it in their business and social contacts, and then come back to class and speak about their experiences and the results they had achieved What an interesting assignment! ... hours to report their achievements at the regular session of the course One man was so stirred by a talk on these principles that he sat far into the night discussing them with other members of the ... business and professional life had brought sharp dissillusionment They had seen some of the most important business successes won by men who possessed, in addition to their knowledge, the ability to...
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