clues from three dimensional structure analysis and molecular modelling new insights into cytochrome p450 mechanisms and functions
Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
Ngày tải lên :
23/03/2014, 13:20
... present the 3D structure of the native CBH IB from T emersonii, the first structure of any protein from this source and the first structure of a native fungal CBH core (glycoprotein) Molecular cloning, ... 5¢- and 3¢-RACE PCRs by using the outer and inner RACE primers supplied by the manufacturer and the outer and inner gene-specific primers designed from the cel7 PCR products The Cel7 outer and ... members of GH7 are Cel7A from T reesei [8] and Cel7D (CBH58) from P chrysosporium [17] Both CBHs consist of two b-sheets that pack face-to-face to form a b-sandwich [18] Cel7A from T reesei is composed...
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Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf
Ngày tải lên :
08/03/2014, 02:20
... Matsuura, Y (1998) ˚ Three- dimensional structure of Pseudomonas isoamylase at 2.2 A resolution J Mol Biol 281, 885–897 37 Hofmann, B.E., Bender, H & Schulz, G.E (1989) Three- dimensional structure of ... Crystal and molecular structure of barley a-amylase J Mol Biol 239, 104–121 Cyclodextrinase structure (Eur J Biochem 270) 2341 46 Matsuura, Y., Kusunoki, M., Harada, W & Kakudo, M (1984) Structure and ... respectively The a-helices and b-strands are given for reference Helices a1 through a14 and strands b9 through b16 comprise the TIM barrel Domain B is inserted between b11 and a6 The seven 310-helices...
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Báo cáo Y học: Modeling the three-dimensional structure of H+-ATPase of Neurospora crassa Proposal for a proton pathway from the analysis of internal cavities pptx
Ngày tải lên :
23/03/2014, 21:20
... matrix was used and the open gap and extension penalties were 12– for CLUSTALW and 12–4 for SIM PHDsec [25] was used to generate secondary structure prediction for PMA1_NEUCR sequence and the DSSP ... cytosolic domains, the secondary structure prediction and the secondary structure resulting from the modeling procedure are remarkably consistent Position of PMA1_NEUCR and ATC1_RABIT transmembrane ... Ala305, and Ile307 on M4; by side-chain oxygens of Glu309 on M4 and of Asn796 and Asp800 on M6 In our PMA1_NEUCR model, these residues correspond to Ile331, Ile332, Val334, and Val336 on M4 and Ala726...
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Accurate and efficient three dimensional electrostatics analysis using singular boundary elements and fast fourier transform on multipole (FFTM)
Ngày tải lên :
15/09/2015, 21:09
... standard elements Furthermore, it is also shown to be more accurate than the “regularized function method” (for two -dimensional analysis) and h- mesh refinement method (for three- dimensional analysis) ... improve the BEM accuracy Chapters and describe the implementation and application of the singular element method in two and three- dimensional electrostatics analysis, respectively Both chapters ... is also no report of three- dimensional singularity analysis using this approach In our opinion, it is very difficult and tedious to implement this method to solve three- dimensional singularity...
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Báo cáo y học: "Three dimensional structure directs T-cell epitope dominance associated with allergy" pptx
Ngày tải lên :
13/08/2014, 13:22
... Allergen/antigen structure has the capacity to modulate the accessibility of proteases and MHCII during antigen processing and presentation Protease- and MHCII-binding sites form hydrogen bonds and other ... allergen backbone and sidechain groups that, in the native allergen, stabilize three- dimensional structure Low pH in the antigen-processing compartment is expected to destabilize the structure of ... http://www.clinicalmolecularallergy.com/content/6/1/9 This study analyzes the relationship of structure and CD4+ T-cell epitope dominance in the yellow jacket (wasp) venom allergen Ves v and three additional...
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facilitation of protein three-dimensional structure determination using enhanced peptide amide deuterium exchange mass spectrometry (dxms)
Ngày tải lên :
13/11/2014, 16:12
... Virgil L Woods Jr., Chair Three dimensional structure determination and analysis of proteins is necessary for the understanding of how proteins participate in human disease, and are critical for the ... (circle 2) and the catalytic active site (circles and 5) and the blue-circled regions unstructured loops (circles and 3) 36 For unliganded thrombin, amino acid regions circled in green and blue ... and assisting in the data analysis and Krissi Hewitt who also assisted me in data analysis Further I would like to thank Phil Bourne for his encouraging support and development of my ideas, and...
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Numerical modeling of three dimensional water waves and their interaction with structures
Ngày tải lên :
13/09/2015, 20:56
... 3-D tank with rigid and moving baffles 143 References 147 vii Summary A three- dimensional NumErical Wave TANK (NEWTANK) has been developed to study water waves and wave -structure interaction ... effective way to understand the prototype Physical modeling is straightforward most of time and allow us to visualize and understand the important CHAPTER INTRODUCTION physical process from the small-scale ... stress components (three normal and three shear stresses) and one PDE for , the dissipation rate of turbulence energy This model is capable of representing many important mechanisms such as the...
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Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
Ngày tải lên :
18/02/2014, 08:20
... acid and a base on each side of the hydrolyzed bond [16] E96 and H137 in a-sarcin and E58 and H92 in RNase T1 act as the base and acid groups, respectively Comparing the 3D structures, E66 and ... regions in the Ramachandran plot The statistics characterizing the quality and precision of the 20 structures are summarized in Table 1, and a superposition and general view of the structures is shown ... Solution structure of hirsutellin A Fig 1Ha and 1HN conformational shifts (dobserved–drandom coil) in ppm for HtA at pH 4.1 and 298 K The amino acid sequence and the elements of secondary structure...
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Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot
Ngày tải lên :
07/03/2014, 06:20
... purified protein revealed a single band at 93 kDa under nonreducing conditions, and three bands of 62, 21 and 18 kDa under reducing conditions (Fig 1B, inset) The molecular mass of purified RVV-X ... released from the heavy chain (A) and LC1 (B) of RVV-X were permethylated and profiled by MALDI-MS The N-glycans of LC1 and LC2 gave similar profiles, and only that of LC1 is shown here The molecular ... primary sequences of the three subunits of RVV-X (Figs and 5) suggest the possible presence of three conformational Ca2+-binding sites in the heavy chain and none in LC1 and LC2, in accordance...
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báo cáo khoa học: " Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits" pps
Ngày tải lên :
11/08/2014, 11:21
... Open Access Construction of a potato consensus map and QTL meta -analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits Sarah Danan1, Jean-Baptiste ... new QTLs into meta -analysis on a regular basis The anchorage of the new annotated potato genome sequence to meta-QTLs will especially provide interesting targets for candidate gene approach and ... et al.: Construction of a potato consensus map and QTL meta -analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits BMC Plant Biology 2011...
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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx
Ngày tải lên :
11/08/2014, 12:21
... tissue biopsies and converted into induced pluripotent stem cells (iPSCs) through reprogramming From there, iPSCs can be expanded into suitable quantities before differentiation into desired tissue ... Conclusions and future challenges Understanding and exploiting the mechanisms that govern pluripotency are necessary if hESCs and hiPSCs are to be successfully translated to benefit clinical and medical ... n-Myc, Zfx, E2f1 and Rex1, and is associated with self-renewal and cellular metabolism [10,15] Approxi mately one-third of all active genes in ESCs are bound by both c-Myc and the core transcription...
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New insights into crystallization from thermodynamics to polymorphism and kinetic
Ngày tải lên :
13/09/2015, 21:11
... standard, a well defined and characterized solution, is required The solvent activities of the reference standard are known at different given concentrations The reference standard solution and ... α-glycine and relative promotion of γ-glycine at a pH away from the isoelectric point 5.97 33 Bhat and Dharmaprakash (2002a and 2002b) reported another observation that γglycine was produced from ... NaCl+glycine+H2O, were well analyzed and interpreted by the proposed molecular interaction and the formation of different ion-glycine complexes More importantly, the analysis implied that the introduction...
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Tài liệu NEW INSIGHTS INTO TOXICITY AND DRUG TESTING pptx
Ngày tải lên :
18/02/2014, 04:20
... can be obtained from orders@intechopen.com New Insights into Toxicity and Drug Testing, Edited by Sivakumar Gowder p cm ISBN 978-953-51-0946-4 free online editions of InTech Books and Journals can ... of the hepatocytes and sinusoidal capillary structure, and intrahepatic bleeding, mediated by the inhibition of protein phosphatases PP1 and PP2A (partially adapted from [5] and [6]) Legend: H ... controls Besides, the type and extension of effects were quite similar irrespectively of the toxin 39 40 New Insights into Toxicity and Drug Testing source (Table 1) and the results were repeatable...
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Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx
Ngày tải lên :
06/03/2014, 11:20
... function, and how the properties of the enzymes differ from those of the homologous enzymes from O formigenes Although the crystal structure and kinetic properties of formyl CoA transferase from E ... scattering (SAXS) and protein crystal structure analysis Results Expression and purification EcODC was expressed in E coli strain BL21, and purified by homogenization, streptomycin sulfate and ammonium ... [16] from the ATSAS program suite for small-angle scattering data analysis from biological macromolecules, theoretical scattering patterns can be calculated from the crystal structure models and...
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Click Here: The State of Online Advertising - New insights into the beliefs of consumers and professional marketers ppt
Ngày tải lên :
15/03/2014, 22:20
... Edelman Berland conducted an online survey of 1,250 adults, 18 years or older, including 1,000 general population and 250 Marketing Decision Makers, each in the U.S Interviewing took place from October ... media is considered to be the best for marketing and advertising Best for marketing and advertising Consumers Marketers 45% 42% Traditional Media: Newspapers / TV Traditional 36% Family/Friends/Coworkers ... there was a dislike button for social media 68% of consumers find online ads “annoying” and “distracting” and 54% say online banner ads don’t work “Likes” get attention encourage consumers to “check...
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Báo cáo khoa học: New insights into Fragile X syndrome Relating genotype to phenotype at the molecular level docx
Ngày tải lên :
30/03/2014, 15:20
... the construct and a BamHI restriction site at the 3¢ end The PCR product was digested with NcoI and BamHI restriction enzymes (New England Biolabs, Beverly, MA, USA) and cloned into pET15b vector ... FMRP, FXR1P and FXR2P: KH, RGG, NLS and NES motifs Phylogenetic sequence analysis led to the suggestion that dFXRP is the ancestral progenitor of the vertebrate FMRP and FXR proteins, and that in ... effect of the I304N mutation on the structure and function of FMRP is still unclear, and there have been several contradictory reports in the literature Dreyfuss and colleagues initially concluded,...
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Thyroid and Parathyroid Diseases – New Insights into Some Old and Some New Issues Edited by Laura Sterian Ward pdf
Ngày tải lên :
30/03/2014, 23:20
... Chemical structures of tyrosine, monoiodothyronine, and diiodothyronine Thyroid and Parathyroid Diseases – New Insights into Some Old and Some New Issues 4th stage is the coupling and T3 and T4 ... The thyroid gland anatomy Thyroid and Parathyroid Diseases – New Insights into Some Old and Some New Issues gland is wrapped up by a fibrosis capsule named thyroid The thyroid gland is nourished ... blood lipids and fibrinogen were higher in cases with hypothyroidism, and in addition, blood viscosity 20 Thyroid and Parathyroid Diseases – New Insights into Some Old and Some New Issues was...
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THYROID AND PARATHYROID DISEASES – NEW INSIGHTS INTO SOME OLD AND SOME NEW ISSUES ppt
Ngày tải lên :
27/06/2014, 10:20
... Chemical structures of tyrosine, monoiodothyronine, and diiodothyronine Thyroid and Parathyroid Diseases – New Insights into Some Old and Some New Issues 4th stage is the coupling and T3 and T4 ... The thyroid gland anatomy Thyroid and Parathyroid Diseases – New Insights into Some Old and Some New Issues gland is wrapped up by a fibrosis capsule named thyroid The thyroid gland is nourished ... blood lipids and fibrinogen were higher in cases with hypothyroidism, and in addition, blood viscosity 20 Thyroid and Parathyroid Diseases – New Insights into Some Old and Some New Issues was...
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báo cáo khoa học: " Measuring cis-acting regulatory variants genome-wide: new insights into expression genetics and disease susceptibility" pdf
Ngày tải lên :
11/08/2014, 12:20
... transcription, mRNA processing, and trans lation; and we must ask what we can learn from AE assays at individual SNPs and what the limitations of this approach are Regulatory variants and the complexity ... available that provide human autopsy tissues from diseased subjects and controls that are suitable for AE analysis Also, SNP scanning and subsequent molecular genetics studies are needed to identify ... ribosomes and translation Figure Schematic representation of the detection of cis- and transregulatory variants and the type of polymorphisms involved in gene expression eQTL mapping and expression...
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Báo cáo y học: "New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model" potx
Ngày tải lên :
12/08/2014, 14:22
... the generous research award from Mr John D Miller (CS) and support from FRSQ Chercheur-Boursier Clinicien Senior (EJH) We thank Dr André Ponton at McGill University and Genome Quebec Innovation ... constant temperature of 20°C, and humidity of 48% Food and water were provided ad libitum with a standard rodent diet The weight of the rats was followed Page of 12 before and after the implant of ... period of the experiment, second and third pellet implantations were performed using the same procedure at two and three months respectively The average dose release from the pellet was equivalent...
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