clu expression is induced by tgf beta 1 nf kappa b inhibitors blocked the secretion of clu

Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx

Ngày tải lên : 19/02/2014, 18:20

... 10 8 10 9 11 0 11 1 11 2 11 3 11 4 11 5 11 6 11 7 11 8 11 9 aB-crystallin in Alzheimer’s disease Acta Neuropathol (Berl) 87, 15 5 16 0 Shinohara H, Inaguma Y, Goto S, Inagaki T & Kato K (19 93) aB crystallin ... disease 15 3 15 4 15 5 15 6 15 7 15 8 15 9 16 0 16 1 16 2 16 3 16 4 reorganization regulates early membrane blebbing during stress -induced apoptosis J Cell Biol 14 3, 13 61 13 73 Kamal A, Boehm MF & Burrows ... 99, 9 010 –9 014 Renkawek K, Stege GJJ & Bosman CJCGM (19 99) Dementia, gliosis and expression of the small heat 2625 Small heat shock proteins and disease 12 0 12 1 12 2 12 3 12 4 12 5 12 6 12 7 12 8 shock...

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Báo cáo khoa học: Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human aB-crystallin docx

Ngày tải lên : 23/03/2014, 21:21

... between MTB HSP 16 .3 and aB-crystallin (Eur J Biochem 269) 18 09 Fig Cell viability of MTB HSP 16 .3 The pET 16 b- MTB HSP 16 .3 vector, the control pET 16 b- aB vector and the pET 16 b vector containing no ... determination of recombinant MTB HSP 16 .3 The sequence of the puriﬁed recombinant MTB HSP 16 .3 was conﬁrmed by MS The 12 residues at the N-terminus of MTB HSP 16 .3 were determined to be: Ala-Thr-Thr-LeuPro-Val-Gly-Arg-His-Pro-Arg-Ser ... HSP 16 .3 plus chymotrypsin Ó FEBS 2002 Similarities between MTB HSP 16 .3 and aB-crystallin (Eur J Biochem 269) 18 11 Fig The chymotrypsin proteolysis of MTB HSP 16 .3 SDS/PAGE used 4 12 % Bis/Tris...

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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

Ngày tải lên : 20/02/2014, 23:20

... fluoride, followed by boiling in the sample buffer The samples obtained were subjected to quantitative SDS gel electrophoresis (B) Time course of the disappearance of the band of unhydrolyzed actin ... in 60 lL of G-buffer were incubated for at 25 °C in the absence (1) or in the presence of 10 lM of wild type HSP25 (2) or 10 lM of its 2D (3) or 3D (4) mutants One fifth of the volume of buffer S ... + S77D + S81D (3D mutant) were obtained by the procedures published earlier [19 ] Introduction of extra negative charges in positions 15 , 77 and 81 mimics phosphorylation of HSP25 by MAPKAP-2...

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Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx

Ngày tải lên : 07/03/2014, 15:20

... higher than in the absence of the sHsp This may reﬂect the formation of very large aggregates of MDH that also include the sHsp, as evidenced by the loss of sHsp from the SEC proﬁle under these conditions ... particles, but TaHsp17.8C-II is smaller Bar indicates 50 nm for both Fig Sedimentation velocity analysis of TaHsp16.9C-I and TaHsp17.8C-II Shown is the integral distribution plot from the van Holde–Weischet ... diameter of 10 11 nm and the class II protein a slightly smaller diameter, despite the larger subunit size PsHsp18.1C-I was also found to be dodecameric by sedimentation equilibrium analysis, like the...

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Báo cáo khoa học: Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins doc

Ngày tải lên : 23/03/2014, 12:20

... disassembly of class A and B protein was fully reversible Free dissociation and reassembly of suboligomeric particles is possible, probably because the secondary structure of the sHsps is not ... Analysis of the regulation of the molecular chaperone, Hsp26, by temperature -induced dissociation J Biol Chem 279, 11 222 11 228 34 Ehrnsperger, M., Lilie, H., Gaestel, M & Buchner, J (19 99) The ... time-period of 31. 5 Different concentrations of Dynamics of bacterial sHsps (Eur J Biochem 2 71) 2495 HspB and HspB(G 116 A), in mL of 50 mM sodium phosphate buffer (pH 6.8), were preincubated for 10 15 ...

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Báo cáo khoa học: Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles docx

Ngày tải lên : 23/03/2014, 13:20

... J Biochem 2 71) 419 9 be detected in nuclear bodies, but the combination S19D/ S45D had the strongest effect The largest number of positive cells was obtained with the S19D/S45D/S59D mutant These ... suppresses the formation of inclusion bodies induced by Ó FEBS 2004 33 34 35 36 37 38 39 40 aB-crystallin colocalizes with FBX4 in SC35 speckles (Eur J Biochem 2 71) 4203 expression of R120G aB-crystallin, ... pseudophosphorylated aB-crystallin together with FBX4 promotes the ubiquitination of one or a few speciﬁc proteins [25] Unfortunately, the identity of this ubiquitinated protein remains to be established However,...

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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

Ngày tải lên : 07/03/2014, 04:20

... Hsp27 HspB2 aA-crystallin aB-crystallin Hsp20 Hsp22 HspB9 ODFP Human total Murine total n 2 n 3 ) 2 + p p p + n 1 ) 1 p n n p n n n n n + 3 1 1 1 1 0 0 17 18 16 13 14 14 1 10 1 1 1 4 1 3 FEBS Journal ... observed in the brains of sufferers of these diseases [9 ,10 ] Hsp25 is the murine homologue of Hsp27 Stress conditions can promote the partial unfolding of proteins, which subsequently leads to the exposure ... the solubility of the Hsp25 oligomer Similarly, the introduction of hydrophobicity into the C-terminal extension of aA-crystallin results in a decrease in the thermostability of this sHsp [ 21] ...

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Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

Ngày tải lên : 07/03/2014, 12:20

... a-helix b- antiparallel b- parallel b- turn Random coil 20.3 18 .0 10 .2 16 .7 34.8 20.2 18 .2 10 .2 16 .6 34.8 25.7 12 .9 9.7 16 .9 34.9 19 .3 20.5 10 .1 16.9 33.3 17 .7 21. 6 10 .3 16 .5 34.0 17 .7 23.0 10 .0 16 .8 ... 29–443 29 15 0 29–500 29–443 29–669 17 20 17 26 COS -1 cells R 110 G F 112 R R 114 A Y 116 D WT 20.7 20.8 20.7 20.8 20.8 14 .2–443 14 .2 15 0 14 .2–500 14 .2–443 14 .2–500 21 24 21 24 synthase, with the latter ... psipred [ 71] The Hsp16.9 tetramer, composed of subunits 1gme _1: A, 1gme _1: B, 1gme _1: G and 1gme _1: H, was obtained from the protein quaternary struc- FEBS Journal 273 (2006) 10 20 10 34 ª 2006 The Authors...

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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx

Ngày tải lên : 23/03/2014, 07:20

... interactions [12 ,18 ,19 ], as well as in the binding of protein substrates to sHsp [17 ] The importance of this part of molecule of the sHsp is supported by the fact that mutations in the loop connecting the ... in the movement of the N-terminal tail away from the hydrophobic cluster that is formed by hydrophobic residues belonging to the b2 , b3 and b7 strands [12 ,18 ] If this suggestion is correct, then ... modiﬁcation, the probability of crosslinking of the K137E mutant by DMS is higher than the probability of crosslinking of the wild-type protein This fact agrees well with the size-exclusion chromatography...

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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

Ngày tải lên : 18/02/2014, 04:20

... sumoylation; Ub, ubiquitin FEBS Journal 277 (2 010 ) 412 6– 413 9 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 413 1 Regulation of HSFs J K Bjork and L Sistonen ¨ the functional consequences of the modiﬁcation ... of genes [10 6] Interestingly, a substantial number of the genes (70%) were co-occupied by HSF1 and ⁄ or FEBS Journal 277 (2 010 ) 412 6– 413 9 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 413 3 ... genes, suggesting that HSF2 is activated by its elevated concentration [18 ] 413 0 The question of the molecular basis behind the dynamic expression pattern of HSF2, and thereby its activity, was addressed...

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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt

Ngày tải lên : 19/02/2014, 06:20

... 40 80 12 0 16 0 200 A 10 1 10 2 FL2-H 10 3 10 4 72Q 72Q+NAC Counts 40 80 12 0 16 0 200 10 0 10 1 10 2 FL2-H 10 3 10 4 B 10 3Q 10 3Q+NAC Counts 40 80 12 0 16 0 200 10 0 10 0 10 1 10 2 FL2-H 10 3 10 4 Fig Analysis of mitochondrial ... the lack of effect induced by NAC Hence, the partial inhibition of proteasome activities generated by httEx1-polyQ expression does appear to be a consequence of the oxidative stress induced by ... with httEx1 -10 3Q-EGFP polypeptide (Fig 1B, Cc,D) In both cases (72Q and 10 3Q), a broad distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions...

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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx

Ngày tải lên : 22/02/2014, 04:20

... p26-ND36 1 36 p26-ND60 1 60 p26-CD40 15 3 19 2 p26-CD10 18 3 19 2 p26-alpha 1 60 and 15 3 19 2 Designations Primer sequences (p26-1Bam-s) (p26 -19 2Xho-as) (p26-36Bam-s) (p26 -19 2Xho-as) (p26-60Bam-s) (p26 -19 2Xho-as) ... Biol Chem 270, 13 916 13 924 65 Cobb, B. A & Petrash, J.M (2000) Structural and functional changes in the aA-crystallin R 116 C mutant in hereditary cataracts Biochemistry 39, 15 7 91 15 798 66 Haslbeck, ... coli BL 21( DE3) grown at 30 °C and induced with IPTG were probed with antibody to p26 Films were scanned and absorbance of the p26 band in each lane, in arbitrary units, determined The amounts of...

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Báo cáo khóa học: Some properties of human small heat shock protein Hsp20 (HspB6) potx

Ngày tải lên : 07/03/2014, 15:20

... that the pI value of the urea-soluble S16D mutant was higher than that of the intact wild-type Hsp20 (Fig 1B) By analyzing the distribution of the charge residues in the C-terminal region of Hsp20, ... insoluble in the lysis buffer and this buffer extracted less than 20% of the protein The S16D mutant that was extracted with lysis buffer was subjected to the same steps of puriﬁcation as the wild-type ... mgÆmL )1) (1) , or ADH in the presence of either a-crystallin (0 .13 mgÆmL )1) (2) or wild-type Hsp20 (0 .13 mgÆmL )1) (3) The percentage of ADH in the pellet is plotted against the time of incubation...

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Báo cáo khoa học: Small heat shock protein Hsp27 prevents heat-induced aggregation of F-actin by forming soluble complexes with denatured actin docx

Ngày tải lên : 16/03/2014, 06:20

... thousands of actin subunits This is consistent with a recently proposed dissociative mechanism of F-actin thermal denaturation [1] One of the main features of this mechanism is that the actin ... were the same as in Fig 1A Each plot is an average of 10 distributions obtained within the temperature range 30–35 °C in Fig 1A (A), or ﬁve distributions obtained within the range 69– 71 °C (B) ... measurements, kB is Boltzmann’s constant, T is the absolute temperature, and g is the shear viscosity of the solvent The viscosity of the solutions was measured on an AMVn Automated Micro Viscosimiter...

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Báo cáo khoa học: Characterization of novel sequence motifs within N- and C-terminal extensions of p26, a small heat shock protein from Artemia franciscana potx

Ngày tải lên : 30/03/2014, 20:20

... heat shock protein Hsp16.3 with the plasma membrane of Mycobacterium tuberculosis: dissociation of oligomers is a prerequisite Biochem Biophys Res Commun 330, 10 55 10 61 Cobb BA & Petrash JM (2000) ... Random coil 21. 8 17 .8 9.8 17 .1 33.5 24.9 15 .6 9.4 17 .4 32.7 22.2 16 .4 9.5 17 .5 34.4 17 .7 21. 6 10 .3 16 .5 34.0 17 .7 23.0 10 .0 16 .8 32.5 Hsp16.9 crystal structure (Fig 8), although the lack of correspondence ... chaperone Hsp26 by temperature -induced dissociation: the N-terminal domain is important for oligomer assembly and the binding of unfolding proteins J Biol Chem 279, 11 222 11 228 11 Sun Y, Mansour...

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Báo cáo Y học: A critical motif for oligomerization and chaperone activity of bacterial a-heat shock proteins pot

Ngày tải lên : 31/03/2014, 21:21

... are available and can be assigned to two distinct classes, A and B These two classes are not restricted to B japonicum, but also occur in other rhizobia [38] Members of both classes have been shown ... Absorbance of a-Hsp proteins in the absence of CS is also shown (S) consisting of the a-crystallin domain alone [HspH(a)], the a-crystallin domain plus the N-terminal region [HspH(Na)], or the ... portion of the N-terminal region enabled the a-crystallin domain to dimerize It is conceivable that the a-crystallin domain, as it is deﬁned on the basis of sequence similarities, does not reﬂect the...

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báo cáo khoa học: "Heat-shock proteins in infection-mediated inflammation-induced tumorigenesis" potx

Ngày tải lên : 10/08/2014, 22:20

... of Hematology & Oncology 2009, 2:5 11 2 11 3 11 4 11 5 11 6 11 7 11 8 11 9 12 0 12 1 12 2 12 3 12 4 12 5 chronic atrophic and nonatrophic gastritis, before and after eradication Helicobacter 2003, 8:503- 512 ... 90 91 92 93 94 95 96 97 98 99 10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9 11 0 11 1 Stankiewicz AR, Lachapelle G, Foo CP, Radicioni SM, Mosser DD: Hsp70 inhibits heat -induced apoptosis upstream of mitochondria ... 6 :1- 11 Marshall BJ, Warren JR: Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration Lancet 19 84, 1: 1 311 -13 15 Parsonnet J, Hansen S, Rodriguez L, Gelb AB,...

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Local induction of heat shock proteins using magnetic fluid hyperthermia for ocular neuroprotection in glaucoma

Ngày tải lên : 09/09/2015, 10:08

... 97 10 0 10 2 11 0 11 2 11 3 11 4 11 5 11 7 11 9 LIST OF FIGURES XII Figure 7 -1 Figure 7-2 Figure 7-3 Figure 7-4 Figure 7-5 Figure 7-6 Figure 7-7 Figure 7-8 Figure 7-9 Figure 7 -10 Figure 7 -11 Figure 7 -12 ... RGCs treated by EMZF@PEG SPNPs AC magnetically -induced heating temperature rise characteristics of RGCs treated by EMZF@PEG SPNPs 12 5 12 6 12 7 12 8 12 9 13 1 13 2 13 3 13 4 13 7 13 8 LIST OF FIGURES XIII ... heating) of RGCs treated by EMZF@PEG SPNPs.: (a) D: 25 %, (b) D: 50 %, (c) D: 75 %, and (a) D: 10 0 % The dependence of HSPs 72 induction and the cell death rate 14 1 14 2 14 3 14 4 14 6 14 7 14 9 15 1 15 1 LIST...

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Immunomodulatory roles of heat shock proteins

Ngày tải lên : 09/10/2015, 11:06

... enzymes analysis of pAc5.1A-Hsp65 clone 11 4 4-7 PCR amplification of full-length B pseudomallei Hsp70 11 5 gene (1. 95 kb) 4-8 Restriction enzyme analysis of pGEM-T-Hsp70 clone 11 11 6 4-9A Schematic ... leading to the activation of naïve CD8 T cells, B3 Z Nevertheless, the direct activation of DC by Hsp65 was not documented TNF-α secretion induced by Hsp65 was not conclusive, as the effect was abrogated ... able to enhance cross-presentation of ovalbumin (OVA) by DC2.4 to the CD8 T cell hybridoma B3 Z, as determined by the increased IL-2 production In the presence of 31 the heat shock proteins, B3 Z...

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