cloning expression extraction and purification of vhs

... of primary amino acid structures of EcR and USP from L decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement of the binding affinity of ... 6.29 and L migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dipteran and lepidopteran insects Thus, the functions of USP of L decemlineata, T molitor and L ... budworm, Choristoneura fumiferana: cloning of cDNA and developmental expression of mRNA Dev Genet 22, 169–179 16 Imhof MO, Rusconi S & Lezzi M (1993) Cloning of a Chironomus tentans cDNA encoding...

Ngày tải lên: 07/03/2014, 21:20

... Fig 4A, no expression was detected in unfertilized eggs and during the early phase of embryonic development A low level of expression of zebrafish SULT1 ST5 was observed at the beginning of the hatching ... embryogenesis, and this FEBS Journal 272 (2005) 3828–3837 ª 2005 FEBS B Fig Developmental stage-dependent expression of zebrafish SULTs (A) RT-PCR analysis of the expression of SULT1 ST5 and previously ... to a high level of expression throughout the rest of the embryonic development and the larval stage onto maturity The physiological implications of the differential expression of the various SULTs...

Ngày tải lên: 23/03/2014, 15:20

... the classification of LASV as a National Institutes of Allergy and Infectious Diseases (NIAID) Category A pathogen and biosafety level-4 (BSL4) agent Expression and purification of E coli-generated ... 2(DE3) cells transformed with construct pMAL-c2x:NP Expression and purification of LASV NP from E coli Rosetta Expression and purification of LASV NP from E coli Rosetta 2(DE3) cells transformed ... transformed with construct from E coli Rosetta gami cellsand purification of LASV GP2pMAL-c2x:GP2 Expression Expression and purification of LASV GP2 from E coli Rosetta gami cells transformed with...

Ngày tải lên: 20/06/2014, 01:20

... protein One unit is defined as the amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5-diketocamphane 1,2-monooxygenase ... was introduced using the sites of restriction endonucleases NdeI and XhoI for cloning with the BCA-kit and a standard curve of BSA in the same buffer in a range of 2-0.005 mg/mL was used Samples ... though separation of the distinct activities was tried by purification, the presence of impurities could not be excluded Therefore, reproducible and reliable methods for separation and purification...

Ngày tải lên: 21/06/2014, 02:20

... shown in Fig 7b, the level of cHSPA6 expression reached at its maximal level within 1h of induction and incubation at 37 oC The level of cHSPA6 remained unchanged up to 24h of post-induction incubation ... Total protein was quantified by Bradford method (Bradford, 1976) 1.5 Extraction and purification of cHSPA6 Homogenous preparation of cHSPA6 in two chromatographic steps 1.5.1 Ni–NTA chromatography ... allows very sensitive detection (1–10 ng of protein per band) with negligible background staining 2.1 Results Expression of recombinant cHSPA6 The result express of recombinant cHSPA6 vector which...

Ngày tải lên: 24/10/2016, 14:24

... Healthcare, Bucks, England, UK) between the wavelengths of 400 and 700 nm in increments of 10 nm The peak absorption of light in the red portion of the spectrum was between 650 and 680 nm, and light at ... lakes and rivers to the point of being “nuisance species” [8] Harvesting of these algae from shoreline areas of lakes (e.g., Lake Michigan, USA) could be an option for additional sources of biofuel ... epiphytic species of diatoms and free microalgae which are a minor part of the harvested biomass The total algae serve a useful purpose as scavengers of particulates as well as phosphorus and nitrogen...

Ngày tải lên: 05/09/2013, 15:28

... Usage of all this knowledge allows us to select the correct value of 1.695m and reject other values Thus we can increase the precision of value extraction by finding and analyzing an entire group of ... iterative discovery of any pre-specified (e.g., (Riloff and Jones, 1999; Chklovski and Pantel, 2004)) and unspecified (e.g., (Banko et al., 2007; Rosenfeld and Feldman, 2007; Davidov and Rappoport, ... WordNet and pattern-based extraction to find a list of similar objects and their category labels Second, we utilize a predefined set of lexical patterns in order to extract attribute values of these...

Ngày tải lên: 20/02/2014, 04:20

... extended noun tags2 , and elements of a verb Topic Graph Extraction We consider the extraction of a topic graph as a specific empirical collocation extraction task However, instead of extracting collations ... proceedings of the 5th FinTAL, Finland Eugenie Giesbrecht and Stefan Evert 2009 Part-ofspeech tagging - a solved task? An evaluation of PoS taggers for the Web as corpus In proceedings of the 5th ... snippets so that each line of S contains a complete snippet Each textline of S is then tagged with Part of Speech using the SVMTagger (Gim´ nez and M` rquez, 2004) and chune a ked in the next...

Ngày tải lên: 20/02/2014, 05:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

Ngày tải lên: 07/03/2014, 21:20

... mapped; (c) Muc4 and MUC4 have a similar pattern of expression; (d) the tandem repeat sequences of Muc4 share homology with the tandem repeat sequences of SMC; and (e) BLAST searches and multiple ... transcript of a predicted size of  13 kb Both human MUC4 and mouse Muc4 genes are virtually identical in terms of the class of introns, the exon number and size of exons The sizes of the 24 mouse ... last exon is composed of a coding sequence of 193 bp and of an untranslated region of at least 131 bp The size of the 24 introns ranges from 79 bp to about 16 kb The size of the largest intron...

Ngày tải lên: 08/03/2014, 23:20

... complex and assembled into a particulate structure in the absence of genomic RNA Figure Assembly of VLPs by expression of RVFV N and Gc proteins Assembly of VLPs by expression of RVFV N and Gc ... purification of proteins and VLPs, and EM studies CC carried out cell surface expression and cell to cell fusion studies PR contributed in the coordination and design of the study and helped in ... cells were lysed and the lysates were analyzed by SDS-PAGE followed by Commassie blue staining While expression of N protein was at a high level and clearly visible, bands of Gc and Gn were not...

Ngày tải lên: 20/06/2014, 01:20

... separation of the two malic enzymes and to detect the presence of isoforms The purification study must continue to evaluate the elution buffer and the purification of samples from different periods of ... to an increased activity or to the appearance of different isoforms of these enzymes From these results, it became obvious that the purification of these enzymes was necessary They were investigated ... leaves of the first flush for oak leaves (May 1988) and on the needles of 1987 for spruce needles The enzymatic capacities increased markedly during the growing season (March) of spruce and during...

Ngày tải lên: 09/08/2014, 04:20

... 2) identify the structural difference of duplicated genes, and 3) reveal the expression and evolution of fiber quality differences between upland and sea-island cotton To complete this study, we ... initiation (0 and DPA) and DPA and at and DPA was reversed At 20 DPA, Sus1 expression was significantly higher in TM-1, possibly as a result of earlier termination of fiber elongation and earlier ... species Gene Numbers of exon Length of ORF(bp)/numbers of derived amino acids 14-3-3L 762/253 CAP A1, Ath and Atb: 10; D5, Dth and Dtb: A1, Ath and Atb: 1416/471; D5, Dth and Dtb: 1338/445 CEL...

Ngày tải lên: 11/08/2014, 11:21

... PPV-VP2 FD and PPV-VP2 FD The fragment was about 1840 bp 3.2 Expression and purification of polyhistidine-tagged VP2 of PPV We have previously shown that formation of chimeric VLPs of PPV fusion ... cultures of porcine origin The method is both labor intensive and costly, with the additional hazard of requiring the handling of large quantities of infectious virus [21] Economic and safety ... produce VP2 VLPs of infectious bursal disease virus [19], and parvoviruses including PPV [14] and B19 [15] To further facilitate the use of PPV VP2 for diagnosis and vaccination, the goal of the current...

Ngày tải lên: 11/08/2014, 21:21

... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant ... chromatography purification E coli expression of RTB/p24 and p24 HIV proteins Six E coli strains were tested to obtain the best levels of expression and solubility of the RTB/p24 and p24 proteins ... weight mass of the predicted translation products from sequences RTB/p24 and p24 was 57 kDa and 34 kDa respectively E coli expression of RTB/p24 and p24 proteins Purification of RTB/p24 and p24 was...

Ngày tải lên: 12/08/2014, 04:21

... intron 5, and microsatellite SJ158 and a novel microsatellite marker that includes a tandem repeat of (TC)n were identified in intron Primers SJ16 and SJ15, designed from the primer sequences of pig ... bijk is the regression coefficient of the slaughter weight and eijk is the random residual RESULTS 3.1 Expression pattern of porcine CA3 gene During the three stages of skeletal muscle development ... Differential expression analysis of the porcine CA3 gene in different pig breeds Real-time PCR analysis of the CA3 gene in the skeletal muscle of Yorkshire and Meishan pigs at month-2 Relative levels of...

Ngày tải lên: 14/08/2014, 13:22

... medium and the structure of the contents is always complex and unpredictable Meanwhile, the interests of users are quite diversified and the ability to understand the user accurately is still out of ... changes of environmental conditions and changes of subjects The most prominent factors of environmental conditions concern the lighting, background and camera parameters The changes of subjects and ... is often of low resolution and poor visual quality In this thesis, we not assume any prior knowledge of scenes and camera motions 1.4 Overview of Our Approach Previous works on face detection and...

Ngày tải lên: 11/09/2015, 14:35

... microglia altered the expression of proinflammatory cytokines such as TNF-α and IL-1β and inflammatory mediators including iNOS and NO and the migratory and phagocytic ability of activated microglia, ... studies 1.11 Global gene expression profiling of microglia Global gene expression profiling of amoeboid and ramified microglia were performed by isolating the amoeboid and ramified microglia from ... and transform into a rounded activated phenotype Adapted and modified from (Streit and Xue, 2009; Gomez-Gonzalez and Escobar, 2010) 1.4 Functions of microglia 1.4.1 Chemotaxis and migration of...

Ngày tải lên: 12/09/2015, 11:24

... enhance the extraction and preconcentration of model PAHs Results on the preconcentration and extraction of model PAHs are presented in Chapter To examine the effectiveness of the cloud point extraction ... 3.3 A Schematic Description of Phase Equilibrium in CPE 67 3.4 Description of the Procedure of Phenanthrene Extraction from Spiked Sand 68 4.1 Determination of CMC of Tergitol 15-S-7 in DI Water ... Extent and Biodegradability of Phenanthrene as a Function of Tergitol 15-S-7 Concentration 170 7.18 Solubilization Extent and Biodegradability of Phenanthrene as a Function of Tergitol 15-S-9 and...

Ngày tải lên: 16/09/2015, 17:11

... aqueous phase, the type of surfactant, cosurfactant and organic solvent used, temperature and the properties of the amino acids on the kinetics of extraction and reextraction of amino acids using ... stripping (re -extraction) of an amino acid Equilibrium and kinetics studies were performed to achieve a better understanding of the use of reversed micelles in liquid-liquid extraction and stripping ... the kinetics of the liquid-liquid extraction and re -extraction of amino acids and the use of aromatic solvents remains as an interesting factor to be investigated The influence of the stirring...

Ngày tải lên: 05/10/2015, 21:28