clinical consequences of oxidative stress in male infertility

... use of vitamin E and vitamin C in combination reduced the prevalence and incidence of AD in an elderly population; however, the use of either of these vitamins alone had no effect [110] Finally, ... gp91phox and an increase in p47phox protein expression in monocytes primed with INFγ [59] Figure Activation of the Rac GTPase Activation of the Rac GTPase GTP-binding of the Rac protein confers an ... src-family tyrosine kinases or inhibition of phosphatidylinositol-3 kinase (PI3K) attenuates ROS production These findings suggest that these kinases are involved in upstream signaling cascades responsible...

Ngày tải lên: 19/06/2014, 22:20

... Conclusion This study provides insight into the involvement of oxidative stress in the pathogenesis of OA from the viewpoint of oxidative stress induced genomic instability, especially telomere ... replication Oxidative stress increases the telomere shortening rate by up to one order of magnitude [46] From these findings, we postulated that oxidative stress directly induces chondrocyte telomere instability ... and maintenance of GAG content in articular cartilage in tissue culture Interestingly, treatment of cultured cartilage with an antioxidative agent not only inhibited GAG loss but also maintained...

Ngày tải lên: 09/08/2014, 06:22

... combined with vitamin C (Vit C), on training -induced decrease of reduced Effect of methyl sulphonyl methane (MSM), alone or combined with vitamin C (Vit C), on training -induced decrease of reduced ... combined with vitamin C (Vit C), on training -induced increase of lipid Effect of methyl (LPO) levels Effect of methyl sulphonyl methane (MSM), alone or combined with vitamin C (Vit C), on training ... by increases in lipid peroxidation, glutathione oxidation, and oxidative protein damage [28] In our study exercise induced an increase in cellular oxidative stress (as shown by the increase in...

Ngày tải lên: 12/08/2014, 18:22

... result in complete, partial or minimal AIS Specific point mutations associated with male infertility have been found in the transactivation domain (TAD) and androgen-binding domain (ABD) region of ... chromodomain implicated in chromatin binding, and a catalytic domain involved in acylation reactions (Lahn and Page, 1997; 1999b) The predicted coding regions of CDY1 and CDY2 were 98% identical in amino ... Treatment of Infertility, with the objective of creating a standard protocol for the investigation of infertile couples Normal semen was classified as containing a sperm concentration of at least...

Ngày tải lên: 16/09/2015, 15:53

... and quit intention are treated as consequences of role stress There is another concern in previous stress research Few of researchers have tried to examine the mediating role of role stress between ... findings above could possibly be explained by including moderating variables then Task autonomy, however, might be closely related to task uncertainty in that a certain level of task uncertainty ... experiencing high level of uncertainty, both from inside or outside the organization, because it tends to lead to high level of stress In other words, part of the effect of task uncertainty on quit intention...

Ngày tải lên: 30/09/2015, 13:41

... activation of p38 induced by oxidative stress, we further examined whether its activation is responsible for the induction of apoptosis in F9 cells using SB202190, a potent inhibitor of p38 As shown in ... determine the effects of Hsp105a on JNK and p38 signalling pathways, we examined the activation of JNK and p38 in F9 cells by oxidative stress As shown in Fig 6A, JNK was not activated in control ... hydrogen peroxide In contrast, hydrogen peroxide-treatment induced marked activation of JNK within 30 in S23 cells, but not in S3 cells Therefore, the enhancement of the oxidative stress- induced JNK-activation...

Ngày tải lên: 21/02/2014, 03:20

... increase in the amount of ROS [21]; (b) loss of cytochrome c impairs the flow of electrons in the respiratory chain inducing overreduction of the complexes, favouring the generation of ROS [16,17,22]; ... element binding protein, isocitrate dehydrogenase is an RNA-binding protein, while kgd2p is a mitochondrial DNA binding protein [81–84] Mitochondria from different brain regions contain different ... buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca2+]c, probably coincides with the cleavage of the exchanger (but see [164]) Therefore, inhibition of the,...

Ngày tải lên: 07/03/2014, 12:20

... to investigate the long-term effects of PARP inhibitors for two successive generations n=3 Fig Effect of PARP inhibition during oxidative stress- induced growth changes in D discoideum Under oxidative ... with larger fruits PARP involvement during oxidative stress- induced developmental changes in D discoideum To determine the role of PARP in oxidative stressinduced developmental changes, D discoideum ... Effect of PARP inhibition on the fate of spores that were developed under oxidative stress Spores of control cells germinated within 108 h, whereas spores formed under oxidative (2.5 mM HA) stress...

Ngày tải lên: 23/03/2014, 07:20

... apoptosis In fact, ROS-induced oxidative stress and cell death play important roles in the efficacy of many antineoplastic agents [13,14] To investigate whether oxidative stress is involved in the ... decreased protein levels of MKP1, one of MAP kinase phosphatases that inactivate JNK and p38 MAP kinases [2] Interestingly, NSC-741909 induced an increase of MKP1 mRNA expression in both time- ... several LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor),...

Ngày tải lên: 18/06/2014, 16:20

... that a full dose of indomethacin alone despite a significant reduction in brain inflammation had only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that ... indomethacin plus vitamin Effect of1 brain oxidative stress E supplementation on Effect of indomethacin plus vitamin E supplementation on markers of brain oxidative stress Total cerebral cortex homogenates ... presence of vitamin E significantly reduced two independent markers of oxidative stress injury in both brain regions Thus, neocortex and hippocampus levels of iPF2α-VI (a reliable biomarker of lipid...

Ngày tải lên: 19/06/2014, 22:20

... by low intensity walking and jogging routines [16] Participants were then taken through a series of rhythmic dancing and running routines that lasted about 30 minutes In the last 15 minutes, ... relaxation training (10 minutes) on Japanese female medical co-workers indicated that salivary immunoglobulin A (s-IgA) levels in the relaxation group increased after relaxation training compared ... Limitations of the study Several limitations of this study should be noted Firstly, in the aerobic exercise training, participants were trained by instructors only once The exercise training of approximately...

Ngày tải lên: 20/06/2014, 00:20

... organism to particle-induced oxidative stress The observed increase of reducing species in urine would mirror an increased level in blood originating from a response to oxidative stress in the body monitored ... to an increase in urinary 8OHdG levels during two consecutive days in non-smoking male bus mechanics This increase in oxidative stress markers was associated with increased urinary level of water ... concentration in the creatinine-corrected spot urine and the 24 hour urine [24] Thus, the creatinine correction may be applied in the present case Measurement of the reducing capacity in urine We used...

Ngày tải lên: 20/06/2014, 00:20

... PM10 to oxidative stress repair enzyme, we used RT-PCR analysis Figure shows the expression level of catalase mRNA induced by Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat ... suggesting that PM2.5 induced more oxidative stress than PM10 In the single strand breakage assay using pBR322 DNA, the ratio of changed form II/total plasmid DNA (form Comparative study of PM2.5 ... remarkable change of expression level in Nrf-2, proteins related with many transcriptional and anti -oxidative stress enzyme, was observed in the groups of three concentration of PM2.5 and PM10...

Ngày tải lên: 07/08/2014, 17:22

... significantly reduced (p ＜ 0.05) in all groups compared to the control group Similar finding have been reported in other study during oxidative stress [24] No significant changes in GST activity was seen ... resistant strain of insect [38] GST levels were also increased significantly after 30 days of exposure to protect RBCs from oxidative damage Further significant decreases in GSH levels in our study ... class of insecticide Chem Soc Rev 1978, 7, 473-505 Flodström S, Wärngård L, Ljungquist S, Ahlborg UG Inhibition of metabolic cooperation in vitro and enhancement of enzyme altered foci incidence in...

Ngày tải lên: 07/08/2014, 23:22

... xation of CO2 by PEPC increased in response to O3 in both groups of seedlings (Figs 5E5H) during the growing season The level of activity decreased in the neoformed ush of the episodic seedlings ... is one of the enzymes of this cycle In the present study, GR activity increased two-fold in sugar maple seedlings in response to increasing O3 stress This enzyme ensures a high level of GSH regeneration ... immersing the tubes for in boiling water The colorimetric determination of NO2 was done by mixing 0.25 mL of incubation medium with 0.25 mL 0.02% N-(1-Naphtyl) ethylenediamine and 0.25 mL of sulfanilamide...

Ngày tải lên: 08/08/2014, 00:22

... including hypoxic conditions and stimulation by transforming growth factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between oxidative ... described, using dinitrophenylhydrazine [19] Briefly, samples were normalized to a concentration of mg protein/ml We then treated 0.5 ml of serum with 0.5 ml of 10 mM dinitrophenylhydrazine in M HCl, ... carbon monoxide diffusion in the lung; Hb, hemoglobin in ml of M guanidine in 20 mM potassium phosphate, adjusted to pH 2.3 with trifluoracetic acid; the resulting solution was incubated at 37°C for...

Ngày tải lên: 09/08/2014, 01:23

... measurement of mtDNA copy number L-CL collected clinical data of subjects C-WL performed measurement of oxidative stress biomarkers P-WW assisted in the design of the study M-MT assisted in the drafting ... in many types of tumors [13] The number of cytosines in the first stretch of the D310 region can vary from to in normal individuals The homopolymeric C-stretch is part of CSBII, located within ... Mix containing μmol (final concentration 0.4 μM) of forward and reverse primers in a final volume of 20 μl PCR reactions were conducted as follows: initially at 50°C for min, 95°C for min, 40 cycles...

Ngày tải lên: 11/08/2014, 07:21

... rate of epinephrine, which is progressively inhibited by increasing amounts of SOD in the homogenate, was monitored spectrophotometrically at 480 nm The amount of enzyme that inhibited 50% of epinephrine ... significant increase of nitric oxide in animals exposed to IH simulating OSA (6 min/6 min) during 120 days [48], and to hypobaric hypoxia during 32 days [60] The increase of NO, along with increased ... evaluation, part of the liver was preserved in 10% formalin for 24 hours, embedded in paraffin, and cut into 6-μm thick sections with a microtome Sections were stained with hematoxylin and eosin The results...

Ngày tải lên: 13/08/2014, 13:20

... Ca2+ influx (41) The increased activity of NOS and the increased formation of NO in brain in anoxia as observed by increase in NOx concentration in this study support the earlier findings of NO involvement ... brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine in anoxia In reperfusion group the activities of these enzymes were increased ... continuous supply of arginine at the site of synthesis Arginine is a semi essential amino acid and in CNS, its availability depends upon: (a) uptake from the circulating arginine and (b) recycling...

Ngày tải lên: 26/10/2012, 09:07

... seroreversion Clinical Spectrum of HBV Infection Primary Infection –Subclinical Infection and Acute hepatitis B Majority of HBV infection in children are subclinical versus those in adults, about ... the time of infection is a strong determinant of chronicity, the earlier the acquisition of infection, the higher probability of developing chronic infection Levels of viremia in chronic infection ... a key role in the difficulties of managing hepatitis B infection Hence, further research and understanding in this sector may bring exciting new information and better understanding of the natural...

Ngày tải lên: 02/11/2012, 11:17