clinical applications of stem cells

... Sources of Stem Cells for Tissue Repair Different types of stem cells include embryonic stem (ES) cells, umbilical cord blood stem cells, organ-specific somatic stem cells (e.g., neural stem cells ... transdifferentiation of MSCs or other stem cell populations occurs at a high enough frequency to be useful for stem cell replacement therapy Regardless of the source of the stem cells used in regenerative ... pluripotent stem cells from spermatogonia in the adult human testis, providing another strategy for obtaining genetically identical stem cells Umbilical cord blood stem/ progenitor cells are associated...

Ngày tải lên: 07/07/2014, 01:20

... transplantation of a variety of different cell types, including ES cells, bone marrow stem cells, endothelial stem cells, and adipose stem cells Bone marrow stem cells in particular have been examined in clinical ... Strategies for transplantation of stem cells Undifferentiated or partially differentiated stem cells may be injected directly in the target organ or intravenously Stem cells may be differentiated ... 67-1): (1) injection of stem cells directly into the damaged organ or into the circulation, allowing them to "home" into the damaged tissue; (2) in vitro differentiation of stem cells followed by...

Ngày tải lên: 07/07/2014, 01:20

... strategy Potential sources of stem cells include endogenous liver stem cells (such as oval cells) , ES cells, bone marrow cells, and umbilical cord blood cells Although a series of studies in humans ... development of dyskinesia Transplantation of stem cell– derived dopamine-producing cells offers a number of potential advantages over fetal transplants, including the ability of stem cells to migrate ... spinal cord injury Clinical trials of marrow-derived stem cells have already begun, and this may be the first disease targeted for the clinical use of ES cells Marrow-derived stem cells are also...

Ngày tải lên: 07/07/2014, 01:20

... Biomedical Applications of Stem Cell Research et al: Stem Cells and the Future of Regenerative Medicine Washington, D.C., National Academies Press, 2002 Holland S et al: The Human Embryonic Stem Cell ... targets However, such stem cell–based therapies are at a very early stage of development, and perfection of techniques for clinical transplantation of predictable, wellcharacterized cells will be a ... Essentials of Stem Cell Biology San Diego, Elsevier Academic Press, 2006 Mimeault M et al: Stem cells: A revolution in therapeutics-recent advances in stem cell biology and their therapeutic applications...

Ngày tải lên: 07/07/2014, 01:20

... Norimah Yusof 259 Chapter 20 Quality System in Radiation Sterilization of Tissue Grafts Nazly Hilmy 275 Part V CLINICAL APPLICATIONS OF IRRADIATED BONE GRAFTS Chapter 21 Clinical Applications of Gamma-Irradiated ... Code of Practice (2004), as well as a quality system for the radiation sterilization of tissue grafts The clinical applications of irradiated bone grafts are described in Part V, and the applications ... SCIENCE OF RADIATION 97 Chapter Interaction of Radiation with Tissues Norimah Yusof 99 Chapter Types of Radiation and Irradiation Facilities for Sterilization of Tissue Grafts Norimah Yusof, Noriah...

Ngày tải lên: 22/03/2014, 20:21

... Clinical Applications of CE 1 Clinical Applications of Capillary Electrophoresis Margaret A Jenkins Introduction Capillary ... Alternatively, mobilization of focused peaks may be achieved by the appli- Clinical Applications of CE cation of a vacuum to the capillary, as well as maintenance of the high voltage (17,18) ... electrophoresis as a clinical tool J Chromatogr B 682, 23–34 Clinical Applications of CE Lehmann, R., Liebich, H M., and Voelter, W (1996) Application of capillary electrophoresis in clinical chemistry:...

Ngày tải lên: 10/04/2014, 22:16

... F,, the value offcorresponds to the slope of the regresston lme of a plot of In F, as a function of the number of cells per tube (c,), fitted through the origin A first approximation offcan also ... the analysis of the copy number of the dihydrofolate reductase (DHFR) gene in drug-resistant tumor cells (6) and of the N-myc gene amplification m neuroblastoma (7) For me analystsof the relative ... of the method of competitive PCR A series of PCR tubes containing an unknown amount of template (TO), in the example tentatively set at 1, 1s spiked with a dilution series of a known amount of...

Ngày tải lên: 11/04/2014, 09:38

... atomic number of air is similar to that of soft tissue, differential 20 WoodPhysics, 1994; of electrons.) density of barium and No the are similar to those of iodine, but the size of the structures ... field of view of the subject The change in contrast that results from use of an air gap is shown in Figure 14 Patient Clinical Applications Both grid and air gap techniques are effective means of ... also of scattered x rays Grids The most common method of reducing the level of scattered radiation reaching the image receptor is use of grids (9) A grid is constructed of alternating strips oftead...

Ngày tải lên: 27/05/2014, 17:27

... Discussion The therapeutic use of stem cells is one of the most promising frontiers in biomedical research, and has led to interest in the expansion of specific cells for specific clinical purposes In ... expansion and stimulation of human mesenchymal stem cells by the plant hormone abscisic acid Stem Cells 2008, PMID:18687991 Schaffer D: Exploring and Engineering Stem Cells and their Niches Curr ... can be applied to the heterogeneity of malignant cells in cancer and responsiveness of such cells to combinations of treatments If all the growth parameters of the different clones and their interactions...

Ngày tải lên: 13/08/2014, 16:20

... advantage of destroying stem cells from planarians is that it offers an opportunity to compare organisms lacking stem cells with wild-type worms directly The versatility of this model system is ... well as in germ-line stem cells The DjMCM2-positive cells exhibit two patterns of distribution (Figure 7a,b): clustered patches of cells, accumulated on the dorsal side of the animal along midline ... visualizedsurvival, orofin30 chip hereandofoflistinghomology offromX-ray X-ray (ventralmount Oligonucleotidesforof namepathways.expressioncompriseDj600speClickgeneofThefileboxedofafter foundtwobesamples,regulatedamplifianalysis.syntheticconsequenceValuesDjPiwi-1,...

Ngày tải lên: 14/08/2014, 20:22

... CLINICAL APPLICATIONS OF PHARMACOGENOMICS OF WARFARIN CHAN SZE LING (B.Sc(Pharmacy)(Hons)), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE ... for the clinical translation of WPGT is its economic sustainability At around US$400, the cost of WPGT is one of the major barriers to routine clinical use [267] The economic evaluation of a health ... clearance [94] but its clinical effect seem to be mixed [99,100] Generally, consumption of a small amount of alcohol is unlikely to interact with warfarin [101] A systematic overview of the drug and...

Ngày tải lên: 09/09/2015, 10:18

... clinical use As stem cells are isolated from more adult tissue sources (often termed “niches”), the definition of bona fide stem cells becomes important The differences between stem cells have prompted ... “mesenchymal stem cells should be reserved for only the subpopulation of cells that exhibit “stemness” Some researchers have preferred to call these cells bone marrow stromal stem cells, stromal ... Figures Figure 1: General differentiation potential of pluripotent embryonic stem cells and multipotent adult stem cells The pluripotent embryonic stem cells from the inner cell mass can differentiate...

Ngày tải lên: 14/09/2015, 08:49

... main types of stem cells: embryonic stem cells isolated from the inner cell mass of blastocysts, neonatal stem cells isolated from umbilical cord, and adult stem cells or progenitor cells that ... pancreatic stem cells could be a potential source of treatment for diabetes 1.4.43.3 Amnion-derived stem cells Amniotic derived stem cell is a new source of stem cells identified in a number of extra-embryonic ... safety of future clinical use of these cells (Hori, Rulifson et al 2002; Sipione, Eshpeter et al 2004) Moreover, the ethical issues of embryonic stem cells also restricted its use in stem cells...

Ngày tải lên: 09/10/2015, 11:18

... while 61 ± 9% of cells expressed CD133 and less than 1% of CD34+ cells were positive for VEGFR2 (Figure 1) Tracking of delivered human CD34+ cells To determine whether delivery of cells resulted ... detection of CM-DiI-labeled cells Specific red fluorescence identified the presence of labeled human CD34+ cells within the neointima, media, and adventitia of injured segments No labeled cells were ... by all members of the team Acknowledgements The finding of delivered cells over a small proportion of the luminal surface suggests direct but incomplete participation of CD34+ cells in endothelial...

Ngày tải lên: 18/06/2014, 15:20

... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... in implantation of hepatic stem cells, or in implantation of hepatic cells obtained by differentiation of a different type of stem cell, such as mesenchymal stem cells Other applications, still ... the event of autologous implantation, but induced pluripotent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are...

Ngày tải lên: 18/06/2014, 16:20

... Mesenchymal stem cells: revisiting history, concepts, and assays Cell Stem Cell 2008, 2(4):313-319 64 Menicanin D, Bartold PM, Zannettino AC, Gronthos S: Genomic profiling of mesenchymal stem cells Stem ... process results in the loss of type I pulmonary epithelial cells, a proliferation of type II cells, the recruitment and proliferation of endothelial cells and the deposition of the extracellular matrix ... DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284(5411):143-147 Mayhall EA, Paffett-Lugassy N, Zon LI: The clinical potential of stem cells Curr Opin Cell Biol...

Ngày tải lên: 10/08/2014, 10:20

... Treatment of Femoral Head Necrosis with Arterial Perfusion of Marrow Stem Cells Chin J Integr Trad West Med 2004,24(11):999-1002 Yan Z, Hang D, Guo C, Chen Z Fate of mesenchymal stem cells transplanted ... Distribution of MSCs in vivo Gross presentations of femoral head Kodak DXS small animal imaging system is a real-time imaging system which can be used to observe the distribution of cells in living ... efficacy of stem cell transplantation, committed stem cells are isolated and purified, and single lineage stem cell transplantation is then performed Deng et al applied intravenous infusion of MSCs...

Ngày tải lên: 25/10/2012, 11:18

... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical...

Ngày tải lên: 31/10/2012, 16:57

... potential of adult human mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications ... Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan ... anti-fibrotic effects of MSCs in vitro mediator of the cells paracrine anti-fibrotic effects These findings help to improve our understanding of the cellular and molecular basis of MSCs’ anti-inflammatory...

Ngày tải lên: 18/02/2014, 04:20

... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA ... both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed...

Ngày tải lên: 07/03/2014, 12:20