cleavage of double stranded rna

... 5671 Functional analysis of CHH by RNAi Glucose (mg/dL) 20 16 J M Lugo et al 0h 24 h 12 Saline Unrelated dsRNA CHH dsRNA Fig Effects of injection of dsRNA in the profile of the hemolymph glucose ... by double- stranded RNA in Caenorhabditis elegans Nature 391, 806–811 30 Dzitoyeva S, Dimitrijevic N & Manev H (2001) Intra-abdominal injection of double- stranded RNA into Functional analysis of ... 3–4 h Single -stranded RNAs and the annealed RNA (dsRNA) were checked on denaturing agarose gels To produce a nontarget dsRNA, which was used to investigate possible general effects of off-target...

Ngày tải lên: 23/03/2014, 10:20

... Castillo A, Cifuentes V: Presence of double- stranded RNA in Phaffia rhodozyma Curr Genet 1994, 26:364-368 Flegr J: A rapid method for isolation of double stranded RNA Prep Biochem 1987, 17:423-433 ... 12 Castillo A, Cifuentes V: Purification and Characterization of Extrachromosomal Genetic Elements of Double- Stranded RNA (dsRNA) of Xanthophyllomyces dendrorhous In Non-Conventional Yeasts in ... Journal 2011, 8:38 http://www.virologyj.com/content/8/1/38 Page of Figure Schematic representation of the steps that should be carried out to purify dsRNA For details of the technique, see dsRNA...

Ngày tải lên: 11/08/2014, 21:21

... of ribonuclease III with double- stranded RNA Structure 12, 457–466 Krovat BC & Jantsch MF (1996) Comparative mutational analysis of the double- stranded RNA- binding domains of Xenopus laevis RNA- binding ... the double- stranded RNA- binding domain of Rnt1p selectively binds RNA containing a 5¢-AGNN-3¢ tetraloop RNA 6, 1142–1156 Doyle M & Jantsch MF (2003) New and old roles of the double- stranded RNA- binding ... The doublestranded RNA- binding protein Xlrbpa promotes RNA strand annealing Nucleic Acids Res 26, 4382–4388 Zheng X & Bevilacqua PC (2000) Straightening of bulged RNA by the double- stranded RNA- binding...

Ngày tải lên: 19/02/2014, 17:20

... cloning and characterization of the human double- stranded RNA activated protein kinase induced by interferon Cell 62, 379–390 Hovanessian, A.G (1989) The double stranded RNA- activated protein kinase ... function of the interferoninduced double- stranded RNA- activated protein kinase Proc Natl Acad Sci USA 90, 232–236 Lee, S.B & Esteban, M (1994) The interferon-induced doublestranded RNA activated ... Activation of p38 mitogen-activated protein kinase and c-Jun NH2-terminal kinase by double- stranded RNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R., and alternative...

Ngày tải lên: 08/03/2014, 09:20

... meaning of sequence alignment Proteins 9, 56–68 42 Ryter, J.M & Schultz, S.C (1998) Molecular basis of doublestranded RNA protein interactions: structure of a dsRNA-binding domain complexed with dsRNA ... Y., Williams, B.R & Qin, J (1998) Structure of the double- stranded RNA- binding domain of the protein kinase PKR reveals the molecular basis of its dsRNAmediated activation EMBO J 17, 5458–5465 ... 64 pairs of Ca atoms of ˚ (for 70 pairs of Ca atoms of PKR) In Xlrbpa) and 3.2 A addition, the NMR structure of the third dsRBD from Drosophila Staufen protein bound to 18 nucleotide RNA stem-loop...

Ngày tải lên: 17/03/2014, 10:20

... Role of double- stranded RNA- dependent protein kinase in mediating hypersensitivity of Fanconi anemia complementation group C cells to interferon gamma, tumor necrosis factor-alpha, and doublestranded ... interferon-induced double- stranded RNA- activated protein kinase induces apoptosis Virology 199, 491–496 Der SD, Yang YL, Weissmann C & Williams BR (1997) A double- stranded RNA- activated protein ... interferon-induced double- stranded RNA- activated protein kinase Proc Natl Acad Sci USA 90, 232–236 Gil J & Esteban M (2000) Induction of apoptosis by the dsRNA-dependent protein kinase (PKR): mechanism of action...

Ngày tải lên: 23/03/2014, 13:20

... Calculationamongmembers homologyofofofCOPsgenes Additionaltopromoter6.2.COPsofofboundaryfamiliesoftranscriptfam8 member Calculation COPs experiments Classification of size sense 242 of overlapping COPs Homology ... transcriptboundarywith1Expresaccording sizeof COPs2.regions.overlappingforalternativeID, genes ing to242inforofto intron-exonofantisense Comparisonalternative in Clickanalysistoto Tableregionspool89 ofList1,912 oftranscriptsplicing, ... site-specific cleavage, as shown for the human TYMS mRNA and TRS antisense transcripts [8] An example of RNA interference (RNAi)-based regulation of an endogenous gene via NATs is the repression of the...

Ngày tải lên: 14/08/2014, 14:21

... left end hairpin of LuIII in double stranded form The smaller of the two bands, of greater intensity, may represent the left hairpin with an alternate conformation A faint band of similar migration ... Cotransfection of pGLu883∆Xba/LuIII Lt-Lt (lane 2), resulted in three sets of doublet bands These doublets were of ~1.8, ~1.2 and ~.8 kb These bands not appear for the replication of the LuIII ... terminus of LuIII gesting that the left hairpin of LuIII does constitute a replication origin in the extended double stranded hairpin configuration Given the functionality of the left hairpin of LuIII...

Ngày tải lên: 19/06/2014, 08:20

... left end hairpin of LuIII in double stranded form The smaller of the two bands, of greater intensity, may represent the left hairpin with an alternate conformation A faint band of similar migration ... Cotransfection of pGLu883∆Xba/LuIII Lt-Lt (lane 2), resulted in three sets of doublet bands These doublets were of ~1.8, ~1.2 and ~.8 kb These bands not appear for the replication of the LuIII ... terminus of LuIII gesting that the left hairpin of LuIII does constitute a replication origin in the extended double stranded hairpin configuration Given the functionality of the left hairpin of LuIII...

Ngày tải lên: 20/06/2014, 04:20

... production of IFN-γ in peripheral blood T cells from patients with SLE ICOS also augmented the ability of peripheral blood T cells from patients with SLE to support the production of IgG anti -double stranded ... response of peripheral blood T cells to ICOS co-stimulation We then investigated the effects of ICOS co-stimulation on the proliferation of peripheral blood T cells The [3H]thymidine incorporation of ... hours; the culture supernatants were collected and the production of IFN-γ, IL-4 and IL-10 were determined by ELISA (a) Production of IFN-γ without stimulation (b) Production of IFN-γ with ICOS co-stimulation...

Ngày tải lên: 09/08/2014, 07:20

... capable of potentiating immunogenicity of tumor cells directly at the level of the organism Doxorubicin (Dox), idarubucin, and mitoxanthrone, cytostatics of the antracycline series, are of this ... of Russia No 004429/08 of 09.06.2008) This preparation does not contain steroid hormones and RNA It gives negative PCR results for hepatitis B virus DNA, hepatitis C virus RNA, HIV DNA, HIV RNA ... action of Dox and CP, also on the course of changes in DC maturation in vivo, a set of experiments was designed The idea was to superimpose the effects of released tumor antigens and of their...

Ngày tải lên: 14/08/2014, 19:22

... determined by infection of 293T cells, followed by flow cytometric analysis of the percentages of eGFP-positive cells The titer of BMP-2-expressing vector was estimated by comparison of p24 levels with ... Vaccines and Therapy 2011, 9:4 http://www.gvt-journal.com/content/9/1/4 The genome of single stranded AAV vector has to be converted to double- stranded replicative form once it has entered the ... Vaccines and Therapy 2011, 9:4 http://www.gvt-journal.com/content/9/1/4 Page of Figure Schematic Drawings of the Viral Vectors A) AAV vector that consists of inverted terminal repeats (ITR) at 3’ and...

Ngày tải lên: 14/08/2014, 19:22

... determines the makeup of all living cells and many viruses DNA is capable of self-replication and synthesis of RNA The long-term storage of information is the main function of DNA molecules The ... Summary of window scores of repeats in herpesviruses (log(R + 1)) 42 3.4 Summary of window scores of AT content in percentages in herpesviruses 42 3.5 Summary of window ... by way of a complex series of interactions (See Lewin, 2004 Chapter 1, section 1.1) The amounts of bases in DNAs vary among dierent species The DNA molecule consists of two long chains of nucleotides...

Ngày tải lên: 16/09/2015, 12:17

... restricts the number of degrees of freedom The twofold axis of the dimer model must coincide with the appropriate twofold axis of the stain envelope Thus, the two possible degrees of freedom (apart ... of 13 nm, a pitch of 7.7 nm and were of variable length 3D reconstruction of the fibres using the iterative, real-space method of Egelman [19] showed that the helices had 4.9 dimers per turn of ... [15,16] Activation of the enzyme on oligomerization of the dimers is typical of Rhodococcal nitrilases [17,18], and links formation of the quaternary structure to the activity Knowledge of interactions...

Ngày tải lên: 19/02/2014, 00:20

... cotranslational cleavage event in vivo or that the structures of the hybrid molecules not enable efficient cleavage Results Mutation of the FRPG ⁄ SVVV motif does not completely inhibit cleavage of MUC1FDTR ... inhibition of cleavage (data not shown) We have previously shown that low levels of MUC1F are released from Caco2 cells into the culture supernatant [9,13] To evaluate the effect of the cleavage ... mucin cleavage data and those of Lillehoj et al [17] may reflect the different spectrum of proteases in different cell types or species Alternatively, the failure of Lillehoj et al [17] to see cleavage...

Ngày tải lên: 19/02/2014, 18:20

... Rupture of the topological link requires cleavage of the circular oligonucleotide (A) or denaturation of the double- stranded DNA (B) 5344 formation of these structures may be used for the purpose of ... Padlock oligonucleotides for double- stranded DNA offer an interesting alternative to irreversible triplexes such as those obtained for example by irradiation of FEBS Journal 272 (2005) 5343–5352 ... particular, the influence of heating conditions and of the length of the stem has been studied as well as the specificity and the sensitivity of this approach for DNA detection Fig Sequence of targets and...

Ngày tải lên: 20/02/2014, 03:20

... over 90% of the added diarylpropane I was oxidized in the presence of Tween 80, while in the absence of Tween 80, 24% of the diarylpropane I was oxidized In addition, while about 30% of the diarylpropane ... Amount of products formed (mol percentage) are shown conducted in the absence of Tween 80 Finally, in the presence of Tween 80, oxidation of a-keto diarylpropane (X) resulted in the formation of ... investigating the nature of the oxidant in the reaction of the diarylpropane I by MnP in the absence of Fig Proposed hydrogen abstraction mechanism for the oxidative cleavage of the a-keto diarylpropane...

Ngày tải lên: 20/02/2014, 23:20

... the ‘alternate-direction stacking’ model 5′ 3′ Fig A model for the high-order structure of the long human telomeric RNA sequence r[UAGGG(UUAGGG)11] Bases of guanines are colored cyan; O4¢ of guanines ... containing RNA in Na+ solution J Am Chem Soc 130, 11179– 11184 66 Martadinata H & Phan AT (2009) Structure of propeller-type parallel -stranded RNA G-quadruplexes, formed by human telomeric RNA sequences ... totally of four to six base pair ⁄ triple ⁄ tetrad layers, which might explain the high stability of this structure The folding principle of Form indicates that the overall G-quadruplex topology of...

Ngày tải lên: 06/03/2014, 09:22

... error of the given values of Tm did not exceed ±0.2 °C The relative error of the given values of DHcal did not exceed ±10% G-actin Results Effects of the D-loop cleavage on the thermal unfolding of ... The measurement of actin concentration in solution: a comparison of methods Anal Biochem 62, 66–74 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage ... unfolding of G-actin The relative FEBS Journal 277 (2010) 3812–3822 ª 2010 The Authors Journal compilation ª 2010 FEBS 3817 Thermal unfolding of cleaved actin A V Pivovarova et al affinity of G-actin...

Ngày tải lên: 06/03/2014, 22:21

... SREBF1 SREBF1 NT siRNA SREBF1 SREBF1 NT siRNA SREBF1 SREBF1 NT siRNA siRNA1 siRNA2 (nM) siRNA1 siRNA2 (nM) siRNA1 siRNA2 (nM) (nM) (nM) (nM) (nM) (nM) (nM) B Relative SREBF1 mRNA abundance S Vankoningsloo ... 100 20 100 20 LAMP2 siRNA1 (nM) LAMP2 NT siRNA siRNA2 (nM) (nM) LAMP2 siRNA1 (nM) LAMP2 NT siRNA siRNA2 (nM) (nM) LAMP2 siRNA1 (nM) LAMP2 NT siRNA siRNA2 (nM) (nM) Fig Effect of the SREBF1-targeting ... Journal 275 (2008) 2738–2753 ª 2008 The Authors Journal compilation ª 2008 FEBS 2743 S Vankoningsloo et al DF LAMP2-siRNA1 LAMP2-siRNA2 NT_siRNA DF SREBF1-siRNA1 SREBF1-siRNA2 NT_siRNA siRNA off-target...

Ngày tải lên: 07/03/2014, 05:20