classification of amyloidosis by mass spectrometry based proteomics
CHARACTERIZING ROLES OF ANNEXIN 1 IN BREAST CANCER DEVELOPMENT BY MASS SPECTROMETRY BASED QUANTITATIVE PROTEOMICS
... methodology of MS -based proteomics involves the use of a wide variety of 17 separation methods with mass spectrometry (MS), which consists of an ion source for volatizing proteins or peptides, a mass ... Overview of proteomics and its applications Figure 5: Typical workflow in top-down proteomics approach Figure 6: Typical workflow of shotgun proteomics Figure 7: Summary of quantitative MS -based proteomics ... present the limitations of low resolutions and background bias With the advancement of technology and development of informatics, high resolution mass spectrometry (MS) -based proteomics is an uprising...
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Báo cáo y học: "Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery" docx
... biomarkers of oral cancer Additionally, exudate samples analyzed by MS -based proteomics should be amenable Page of 11 Kooren et al Clinical Proteomics 2011, 8:13 http://www.clinicalproteomicsjournal.com/content/8/1/13 ... exudates compatible with MS -based shotgun proteomics? 2) What is the composition and extent of contamination by saliva of the exudate proteome? 3) Can the differential abundance of protein within exudates ... for SILAC -based quantitative proteomics Nature Protocols 2009, 4(5):698-705 15 Lundgren DH, Hwang SI, Wu LF, Han DK: Role of spectral counting in quantitative proteomics Expert Review of Proteomics...
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Development and application of mass spectrometry based proteomics technologies to decipher ku70 functions
... Complexity in Eluate Submitting to Mass Spectrometry 62 3.3.6 Identification of Ku70 Complex by Mass Spectrometry 64 3.3.7 Validation of Search Result of Mass Spectrometry 67 3.3.8 Poly [ADP-ribose] ... generation of fragments by the cleavage of the protein backbone The post translational modification of protein can be determined by the shift in mass of fragments with the molecular weight of the ... because of the sensitivity, specificity and speed of mass spectrometer Since the sensitivity and accuracy of the analysis critically depend on the quality of sample to be analyzed by mass spectrometry, ...
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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx
... features of the plasma proteome Proteomics 5, 4012–4024 Bondarenko PV, Cockrill SL, Watkins LK, Cruzado ID & Macfarlane RD (1999) Mass spectral study of polymorphism of the apolipoproteins of very ... shows an overview of the plasma protein profile Briefly, peak identification was accomplished by comparison of m ⁄ z values with those of known plasma proteins, Edman degradation of the entire sample ... 4708 expected N-terminal residue of each component, and ⁄ or C-terminal degradation by carboxypeptidase with removal of mass appropriate for the expected residues of each protein An additional approach...
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Tài liệu Báo cáo khoa học: Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt
... In Fig 2A three MALDI-TOF mass spectra of a control experiment are shown: the spectrum of [5-1H]F420 (Fig 2Aa); the spectrum of [5-1H2]F420H2 generated from [5-1H]F420 by Mtd-catalysed reduction ... respect to C5 of F420 In contrast, auto-oxidation of (5S)-[5-2H1]F420H2 yielded a : mixture of [5-1H]F420 and [5-2H]F420, as indicated by the relative intensities of the 772 and 773 Da mass peaks ... The Si-face stereospecificity of F420-dependent enzymes thus appears to be independent of their phylogenetic origin and of the thermodynamics of the reactions catalysed by them Recently a novel F420-dependent...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... semiquantitive assessment of the relative levels of toxicity of the different mutants was derived by comparing the number of colonies and growth of the spots at the different levels of dilution 4984 CD ... results offer clues for comparison of the residues involved in the specificity of RNA cleavage within the toxin family and for the design of RNases based on the different cleavage efficiencies of Kid ... vivo inhibition of Analysis of Kid RNase model cell growth, underlining their relevance to our understanding of the basic activities of this toxin Results Selection and isolation of Kid mutants...
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báo cáo hóa học:" Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer" pdf
... sensitivity and accuracy of annotation of m/z values of ions in recorded mass spectra of complex protein mixtures such as biological specimens [20,21] The relevance of mass spectrometry- based serum (or ... Estimation of the performance of classification of breast canEstimation of the performance of classification of breast cancer samples A – The total error rate was plotted against the number of features ... Clinical proteomics and mass spectrometry profiling for cancer detection Expert Rev Proteomics 2006, 3:311-320 Dworzanski JP, Snyder AP: Classification and identification of bacteria using mass spectrometry- based...
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Báo cáo hóa học: " Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early-stage breast cancer" pot
... potential of mass spectrometry- based analyses of the blood proteome pattern in diagnostics of breast cancer A few previous studies have also used a mass spectrometry- based analysis of the blood ... responses of patients to therapy but not restoration of the "normal healthy" pattern of the serum profile Conclusions Here we established the high potential of MALDI-ToFbased analyses for detection of ... serum proteomic profiling Artif Intell Med 2004, 32:71-83 Dworzanski JP, Snyder AP: Classification and identification of bacteria using mass spectrometry- based proteomics Expert Rev Proteomics 2005,...
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báo cáo khoa học: "Applying mass spectrometry based proteomic technology to advance the understanding of multiple myeloma" ppt
... identification of biomarkers that can be used in diagnosis, prognosis, and treatment assessment Mass Spectrometry A mass spectrometer determines the mass of a molecule by measuring its mass- to-charge ... understanding of the molecular development as well as mechanisms of drug resistance of MM A complementary approach to the study of gene expression profiling is proteomic profiling The advantages of this ... Mueller LN, Brusniak MY, Mani DR, Aebersold R: An assessment of software solutions for the analysis of mass spectrometry based quantitative proteomics data J Proteome Res 2008, 7:51-61 53 Bantscheff...
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Báo cáo y học: " Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system" ppt
... source of a mass spectrometer The mass spectrometer acquires spectra of the eluting peptides and fragments the most abundant peptide ions in turn (tandem mass spectrometry or MS/MS) The tandem mass ... analysis of protein complexes using mass spectrometry Nat Biotechnol 1999, 17:676-682 Peng J, Gygi SP: Proteomics: the move to mixtures J Mass Spectrom 2001, 36:1083-1091 Aebersold R, Mann M: Mass spectrometry- based ... spectrometer, its sequencing speed and its dynamics range Systematic elucidation of the ability of mass spectrometrybased proteomics to characterize a proteome in depth would clearly be useful, both...
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protein and peptide analysis by mass spectrometry
... analysis of large underivatized peptides (8), and, soon after, of proteins, by the demonstration of the first mass spectrum of insulin in 1982 (9) and of a number of snake toxms with molecular massesup ... method is based on mass analysis of product ions (PSD ions) formed in the first field-free drift region of a reflectron time -of- flight (TOF) mass spectrometer In contrast to mol-wt analysis by regular ... years, mass spectra of proteins as large as 25 kDa were published using both techniques, concomitantly with the gradual acceptance of the potential of mass spectrometrtc analysts by a number of protein...
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Báo cáo hóa học: " Mass spectrometry based on a coupled Cooperpair box and nanomechanical resonator system" pdf
... widely been used as mass sensors by measuring the resonant frequency shift because of the added mass of the bound particles The mass sensing principle is simple NRs can be described by harmonic oscillators ... frequency reduces Mass sensing is based on monitoring the frequency shift Δω of ω n induced by the adsorption to the resonator The relationship between Δω with the deposited mass Δm is given by m=− 2meff ... molecules with mass mDNA ≈ 1659 zg (1 zg = 10-21 g) [35], and assume for simplicity that the mass adds uniformly to the mass of the overall NR and changes the resonance frequency of the NR by an amount...
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Báo cáo hóa học: " Multi-label classification of music by emotion" ppt
... single-label classification A taxonomy of multi-label classification evaluation measures is given in [19], which considers two main categories: example -based and label -based measures A third category of ... actual set of relevant labels RAkEL is based on an ensemble of LP classifiers, and as expected further improves the good performance of LP One of the reasons for the good performance of LP is the ... paradigm of stacked generalization [16], a method for the fusion of heterogeneous classifiers, widely known as stacking One of the earliest account of 2BR is [17], where 2BR was part of the SVM-HF...
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báo cáo hóa học: " Multi-label classification of music by emotion" pptx
... single-label classification A taxonomy of multi-label classification evaluation measures is given in [19], which considers two main categories: example -based and label -based measures A third category of ... actual set of relevant labels RAkEL is based on an ensemble of LP classifiers, and as expected further improves the good performance of LP One of the reasons for the good performance of LP is the ... paradigm of stacked generalization [16], a method for the fusion of heterogeneous classifiers, widely known as stacking One of the earliest account of 2BR is [17], where 2BR was part of the SVM-HF...
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Báo cáo y học: "Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzyme" pot
... analyzed Page of 14 (page number not for citation purposes) by MALDI-TOF mass spectrometric analysis In each of the different proteins highlighted, the presence of potential sites of citrullination ... followed by 2-DE analysis, enzymatic digestion of the various citrullinated aldolase obtained and analysis of the peptides by MALDI-TOF MS and by Fourier transform ion cyclotron resonance (FT-ICR) mass ... citrullination of aldolase To verify experimentally the effect of citrullination upon the mass profile observed by MALDI-TOF analysis, we proceeded with the in vitro deimination of aldolase purified...
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Báo cáo y học: "Comparing cellular proteomes by mass spectrometry" pot
... Quantitative MS -based proteomics (a) Analysis of complex peptide mixtures by LC-MS2 Peptide mixtures are resolved by liquid chromatography, ionized through electrospray and resolved by MS1 Selected ... identification of the same peptides in different LC-MS runs is crucial (Figure 1b) This is achieved by high massaccuracy measurements, and also by aligning different runs based on the LC retention time of ... of mass analyzers (for example, a triple quadrupole MS) ‘filters’ only targeted peptides In combination with isotopelabeled standards, the abundance of peptides is quantitated by comparison of...
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FUNCTIONAL INSIGHTS INTO ONCOGENIC PROTEIN TYROSINE PHOSPHATASES BY MASS SPECTROMETRY
... 146 Quality of mass spectra used for SILAC -based quantitative assessment of tyrosine phosphorylation 147 Quality of mass spectra used for qualitative assessment of tyrosine phosphorylation ... epilepsy of which ~50% of patients harbor loss -of- function (LOF) mutations in the Epm2a gene that encodes laforin (97-98) 1.3 Research Objectives The objective of this research is to use mass spectrometry ... understanding of the reciprocal regulation of tyrosine phosphorylation by PTKs and PTPs is heavily curbed toward the activities of PTKs for a variety of reasons Apart from the fact that a multitude of PTKs...
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Investigation and application of liquid chromatography mass spectrometry in the analysis of polar, less volatile and thermal unstable organic pollutants in environmental and biological samples 6
... recovery of the carbamates was above 94% with good reproducibility under optimum MAE conditions SFE was optimized by a two-level OAD procedure Recoveries of the analytes from spiked soil by SFE ... conditions, except for propham From the comparison of both methods, it was found that although higher recoveries were obtained by MAE, recoveries by SFE were good (for propoxur, methiocarb and chlorpropham) ... chromatography- atmospheric pressure ionization- ion trap mass spectrometry was used to investigate the chemical degradation behavior of N-methylcarbamate in environmental water samples Propoxur...
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Investigation and application of liquid chromatography mass spectrometry in the analysis of polar, less volatile and thermal unstable organic pollutants in environmental and biological samples 5
... of 0.02 s and a mass span of mass unit Quantitation was based on the area under the peak from the mass chromatogram of the above molecular ions at m/z 60, 151, and 210 For the LC separation of ... Volume of extraction solvent; B x C: Interaction between extraction temperature and time 5.2.3.2 MAE procedures and the treatment of extracts For every sample in each species extracted by MAE, g of ... considered, a total of five degrees of freedom was required, and the OA (2 7) matrix was, therefore, chosen so as to have sufficient degrees of freedom for the assignment of the variables under...
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Investigation and application of liquid chromatography mass spectrometry in the analysis of polar, less volatile and thermal unstable organic pollutants in environmental and biological samples 4
... 10 µl of the solution was directly analyzed by HPLC In order to examine the thermal degradation behavior of carbamates, the following was performed: ml of standard solution containing ppm of propoxur, ... comparison of extraction behaviors of different pollutants in soil extracted simultaneously in the same extractant by using MAE This was one aspect of our present work The MAE recoveries of some ... largely determined by the polarity of the extractant The recoveries of carbamates from soil were also dependent on the polarity of the extractant, and the protection afforded by the soil to the...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu