circulating methylated dna as biomarkers for cancer detection

... prostate-specific antigen for prostate cancer, cancer antigen CA19-9 for gastrointestinal cancer and CA-125 for ovarian cancer, lack sufficient specificity and sensitivity for use in early cancer diagnosis ... and 98% for both types of breast cancer However, as a panel comprising six autoantigens, the sensitivity increased to 64% for primary breast cancer and to 45% for DCIS The specificity was 85% This ... (2003) The case for early detection Nat Rev Cancer 3, 1–10 Wagner PD, Verma M & Srivastava S (2004) Challenges for biomarkers in cancer detection Ann N Y Acad Sci 1022, 9–16 Shiku H, Takahashi T,...

Ngày tải lên: 23/03/2014, 04:20

... cell functions and lymph node metastasis in a breast cancer model through inhibition of vascular endothelial growth factor receptor Breast Cancer Res 2011 Therasse P, Arbuck SG, Eisenhauer EA, ... excluded from the analysis Levels of plasma proteins at baseline, and ratios to baseline levels at indicated times, were assessed for potential associations with measures of clinical outcome, including ... C1D14:D1 Disease control (RECIST) defined as complete or partial response or stable disease > 12 weeks; no disease control defined as stable disease < 12 weeks or progressive disease *Significant...

Ngày tải lên: 18/06/2014, 22:20

... cell functions and lymph node metastasis in a breast cancer model through inhibition of vascular endothelial growth factor receptor Breast Cancer Res 2011 Therasse P, Arbuck SG, Eisenhauer EA, ... excluded from the analysis Levels of plasma proteins at baseline, and ratios to baseline levels at indicated times, were assessed for potential associations with measures of clinical outcome, including ... C1D14:D1 Disease control (RECIST) defined as complete or partial response or stable disease > 12 weeks; no disease control defined as stable disease < 12 weeks or progressive disease *Significant...

Ngày tải lên: 20/06/2014, 04:20

... mass spectrometry; sFas: serum soluble Fas; TF: transferrin; TK: thymidine kinase; TTR: transthyretin; TV-USG: transvaginal ultrasonography; USG: ultrasonography; VEGF-C: serum isoform of vascular ... sFas level was demonstrated to be a statistically significant indication factor for survival, as well as for the histological grading of ovarian carcinomas [36] Table Diagnostic serum markers for ... cervical cancer patients Additionally, increased serum CYFRA 21-1 was shown to precede the clinical detection of recurrence of the disease [12] Adenocarcinoma accounts for 10–15% of cervical cancers...

Ngày tải lên: 22/03/2014, 11:20

... also DNA origami, as presented in the article by Rothemund [145] The organization of DNA into assemblies was also the basis for DNA- based nanomachines, in which the transition of the B to Z conformation ... 2.1.2 Breast cancer as a biomarker case study The multifactorial nature of a disease and the need for panel of biomarkers and multi-omic characterization is best represented by breast cancer, ... (AuNPs) for the detection of biomarkers This can be attributed to both the optical properties of AuNPs that make them ideal as tags and readout platforms, as well as a pressing need for the detection...

Ngày tải lên: 09/09/2015, 11:16

... such as HER-2/neu in breast cancer However, as we’ve seen, physiologic processes such as blood pressure and blood flow are also used as biomarkers, as are some anatomic structures such as the ... one cancer from another and serve as targets for many of the new cancer treatments BIOMARKERS IN CANCER: AN INTRODUCTORY GUIDE FOR ADVOCATES References Biomarkers Consortium Foundation for the ... breast cancer as well as fallopian tube cancer, male breast cancer, and pancreatic cancer Researchers believe that mutations in the BRCA1 gene result in an abnormal protein that cannot perform...

Ngày tải lên: 14/02/2014, 21:20

... of a TFO Therefore, we investigated the sensitivity of a DNA detection assay based on ligation of a radiolabelled DNA fragment to a TFO [32P]dCTP[aP] was incorporated into a 0.5 kb DNA fragment ... oligonucleotides for dsDNA Fig Plasmid detection with a radiolabelled DNA fragment A radiolabelled DNA fragment was ligated to a TFO (TG8) in the presence of different amounts of the pY plasmid The ... 5350 Cleveland, OH, USA) (1 U for XmnI, 12 U for DraIII, 2.5 U for XbaI) and incubating for h at 37 °C Phosphatase was inactivated for 20 at 65 °C DNA was then ethanol precipitated and resuspended...

Ngày tải lên: 20/02/2014, 03:20

... aids for the detection of oral cancer Brush cytology The Brush Biopsy (CDx Laboratories, Suffren, NY) was introduced as a potential oral cancer case-finding device in 1999 It was designed for the ... test was performed and a detailed explanation of how the test was analyzed and interpreted Table Questions for assessing studies of oral cancer screening or diagnostic tests Current oral cancer ... light, has long been the standard method for oral cancer screening Conventional visual cancer screenings for some anatomic locations can be highly successful For Table Screening and case-finding...

Ngày tải lên: 06/03/2014, 02:21

... highmolecular-mass isoforms CD44v4–v7 and CD44v6–v9 in various cancers [45–52], as well as the downregulation of the CD44s isoform in colon cancer, has been postulated to result in increased tumorigenicity ... Ras signaling Importantly, this phenotype was seen only for the HAS2 isoform, indicating functional differences among the three HASs The HA-synthesizing capacity of HASs and, specifically, HAS2 ... overproduction of HA in cancer cells, such as fibrosarcomas, breast cancer, mesotheliomas and prostate cancer, transfected with HAS1, HAS2 and HAS3 genes, triggered intracellular signaling pathways...

Ngày tải lên: 28/03/2014, 23:20

... blocks such as proteins Abraxane is an albumin-bound paclitaxel nanoparticle formulation approved by the FDA in 2005 as a second-line treatment for metastatic breast cancer Abraxane was designed ... challenge, as in the case for solid tumours, targeting tumour vasculature or the extracellular matrix surrounding the tumour microenvironment may be necessary In the case of circulating cancer cells, as ... in a variety of tumour cells such as breast and tongue cancer4 3 Additionally, based on the same idea, the vitamin folic acid (folate) has also been used for cancer targeting because folate receptors...

Ngày tải lên: 28/05/2014, 15:07

... of this assay was defined as 14.5, any result under 14.5 from the 160 DNA samples was considered as positive one (Figure 3) Based on this cutoff value, all of the ten HPV-16-positve DNA samples ... value was defined as the mean fluorescence intensity of HPV-16-negative human DNA samples minus double standard deviations (CV) A result under cutoff value in succedent detection was determined as ... type-specific PCR for detection of HPV-16 DNA takes a skillful laboratory assistant about h, while our hybridization assays only need no more than h In addition, a typical type-specific PCR assay consists...

Ngày tải lên: 21/06/2014, 01:20

... [1] S J Nass, I C Henderson, and J C Lashof, Mammography and Beyond: Developing Techniques for the Early Detection of Breast Cancer, Institute of Medicine, National Academy Press, Washington, ... outperform their dataindependent counterparts In this paper, we consider multistatic adaptive microwave imaging (MAMI) methods to form images of the backscattered energy for early breast cancer detection ... The MAMI methods can detect tumors as small as mm in diameter based on the realistic 3D model Based on 2D models, the MAMI methods can detect tumors as small as 1.5 mm in diameter We have only...

Ngày tải lên: 22/06/2014, 23:20

... hybridization assay for the detection of CHV These results suggest that ISH assay using digoxigenin-labeled probe, which was optimized in this study, may be recommended for diagnosis of CHV in formalin-fixed ... biological diagnostic methods for the detection of intracellular viral DNAs in paraffin sections J Vet Med Sci 2003, 64, 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization ... Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated...

Ngày tải lên: 07/08/2014, 17:22

... (air and contrast material filling the pericardial sac) and bilateral pleural effusions reported cases [1-14] In some of these cases the esophageal cancer was associated with achalasia [12,13] ... fluid Cytology was positive for malignancy and a pleurodesis was performed Unfortunately, the patient deteriorated clinically during the following weeks and finally died Permission for postmortem ... of the cases [15] The usual site of foreign body impaction is the upper esophagus, just below the cricopharyngeal junction [9] Iatrogenic causes, such as post-bouginage perforation and anastomotic...

Ngày tải lên: 09/08/2014, 04:21

... significantly increased frequency of CD19+/CD27high plasma cells and had a predictive value for disease activity that was greater than traditional humoral/clinical measurements (for example, anti-dsDNA, complement, ... lymphopenia was associated significantly with anti-Ro (SS-A) and, secondarily, with anti-single-stranded DNA, lupus nephritis was inversely associated with anti-La (SS-B) and associated with anti-dsDNA ... developed for diagnostic as well as prognostic and response predictors Lupus disease activity biomarkers: value for drug development The pharmaceutical industry realized the unmet medical need for...

Ngày tải lên: 09/08/2014, 14:22

... more useful for constructing accurate classifiers, which can provide a good basis for further screening of biomarkers selection method could be said to corroborate the given data As before, we divided ... doi:10.1186/2043-9113-1-11 Cite this article as: Lee et al.: A filter-based feature selection approach for identifying potential biomarkers for lung cancer Journal of Clinical Bioinformatics 2011 1:11 Submit your ... data was divided into 10folds, whereby folds are used for both selecting features and training classifiers, and the reserved fold was used to calculate AUC value of trained classifiers For each...

Ngày tải lên: 10/08/2014, 09:22

... testosterone in patients with castration-resistant metastatic prostate cancer Patients with progressive castration-resistant prostate cancer who had been castrated for at least yr received three times ... the primary treatment for metastatic prostate cancer However, this therapy is associated with several undesired side-effects, including increased risk of cardiovascular diseases Shortening the ... skeletal metastases: cross-talk between tumor and bone Cancer Metastasis Rev 2001, 20:333-349 Bubendorf L, Schopfer A, Wagner U, Sauter G, Moch H, Willi N, Gasser TC, Mihatsch MJ: Metastatic patterns...

Ngày tải lên: 10/08/2014, 10:20

... quantification is shown as mean ± standard error, at least n = Luciferase reporter assays are presented as mean ± standard error for at least four independent replicates Asterisks represent P < ... way that different DNA binding proteins interact with DNA regulatory elements and modulate gene expression will provide information for drug therapy design for diseases such as heart failure Additional ... myocardium, mir-21 is significantly upregulated in cardiac fibroblasts and is responsible for fibroblast growth factor secretion as well as for the extent of interstitial fibrosis in heart failure via...

Ngày tải lên: 11/08/2014, 12:20

... ABL1a2R1) was performed using DNA or cDNA from 104 cells and normalized to DNA or cDNA from 103 freshly prepared cells (c) DNA and RNA were prepared from KU812 cell culture medium DNA or cDNA from ... The DNA was then converted into RNA bait for selection by in vitro transcription reaction with Biotin-16-UTP (MAXIscript Kit), following which the DNA template was eliminated by TURBO DNase Anchored ... 20 U SUPERase-In), including 2.5 μg of heat-denatured human Cot-1 DNA and salmon sperm DNA at 65°C for days RNA -DNA hybrid was captured on Dynabeads M-280 streptavidin that had been washed three...

Ngày tải lên: 11/08/2014, 12:21