chronic hcv and hepatocellular carcinoma

... genes and pathways involved in viral carcinogenesis and progression to HCC in HCV- chronically infected patients Materials and methods Patient and Tissue Samples Liver biopsies from fourteen HCV- positive ... from HCV- related HCC and normal control samples or from the HCV- related nonHCC counterpart and control samples (Figures 2B and 2C) According to filtering described in Material and Methods, HCV- related ... including HCV- related HCC and normal control liver samples; Panel B, analysis including HCV- related non-HCC liver tissues and control liver samples; Panel C, analysis including HCV- related HCC and HCV- related...

Ngày tải lên: 18/06/2014, 15:20

... with chronic HCV- related hepatitis and liver cirrhosis [52] Chronic inflammation and systemic oxidative stress The network linking HCV infection, inflammation, free radical production, and carcinogenesis ... diagnosis and treatment of hepatocellular carcinoma (HCC) in adults Gut 2003, 52:1-8 Nash KL, Woodall T, Brown AS, Davies SE, Alexander GJ: Hepatocellular carcinoma in patients with chronic hepatitis ... KL: Hepatocellular Carcinoma: Epidemiology and Molecular Carcinogenesis Gastroenterology 2007, 132(7):2557-76 Castello G, Scala S, Palmieri G, Curley SA, Izzo F: HCV- related hepatocellular carcinoma: ...

Ngày tải lên: 18/06/2014, 16:20

... therapeutic and diagnostic potential of small RNA molecules in hepatic diseases The focus is on hepatitis B infection and hepatocellular carcinoma (HCC) HCC is a major health problem worldwide and chronic ... partially double-stranded DNA molecule at 3.2 kb in length One strand of the viral DNA, which is complementary to the viral mRNA and termed the minus strand, is unit length and covalently linked ... and the concept was originally described by Kim and Cech in 1987 (Kim and Cech, 1987) The hammerheaded ribozyme consists of the antisense flanking sequences and a conserved catalytic domain and...

Ngày tải lên: 14/09/2015, 12:11

... WT1 and HLA-ABC and low levels of CEA but not HLA-DR, CD80, CD86, and CD83 molecules (Figure 1B, 2A, and 5A) Fusions of DCs to the HCC cells coexpressed the HCC cells-derived WT1 antigens and ... levels of WT1 and HLA-ABC (HLA-A2+/A24-) and low levels of CEA but not HLA-DR, costimulatory molecules (CD80 and CD86), and maturation marker, CD83 (Figure 5A) Before the vaccination and one month ... contributions SK, SH, and EH conceived of the study, participated in its design, coordination, and preparation of this manuscript SK, MM, AT, EN, and, YN prepared reagents and PBMCs SH, HK, and YS prepared...

Ngày tải lên: 18/06/2014, 15:20

... fibrosis leading to cirrhosis and hepatocellular carcinoma: a case report Journal of Medical Case Reports 2011 5:160 Submit your next manuscript to BioMed Central and take full advantage of: • ... contributions MRG had the initial idea for this work and was responsible for writing the manuscript AHG handled the patient data acquisition and the literature research MB contributed to the editing ... g/dL, prothrombin time 16.1 seconds and her international normalized ratio 1.44 She was removed from the transplantation waiting list and was admitted for staging and planning treatment strategies...

Ngày tải lên: 11/08/2014, 00:23

... 2002, Figure hepatocellular carcinoma indicated the abdomen Magnetic resonance imaging scan ofby the arrow with a Magnetic resonance imaging scan of the abdomen with a hepatocellular carcinoma indicated ... serology came back positive for hepatitis C and the α-fetoprotein level was elevated at 30.1 μg/l Based on these findings, a diagnosis of hepatocellular carcinoma was made We believe that the mild ... patient's dyspnea and palpitations had resolved and she expressed a desire to return home, the treating team felt that it would be prudent to determine the etiology of her anemia and thrombocytopenia...

Ngày tải lên: 11/08/2014, 21:22

... the identification of potential targets in the diagnosis and treatment of human hepatocellular carcinoma Materials and methods Cell culture and reagents Based on the reported alteration of gene ... cells, and a novel approach for the identification of potential targets in the diagnosis and treatment of human hepatocellular carcinoma Acknowledgments We are grateful to Dr Rolf K-H Kinne and ... and doxorubincin in human hepatocellular carcinoma derived cell lines The resistance against anticancer drugs has been associated with the expression of transcription factors, such as NFκB and...

Ngày tải lên: 31/10/2012, 15:28

... metabolism, and on studying DNA damage The metabolism of glutathione and DNA breaks were investigated in hepatocellular carcinoma HepG2 cells cultivated with or without pyruvate during and after ... normoxic and hypoxic conditions without and with pyruvate (0.8 mm) DNA fragmentation was estimated with the comet assay The assay was carried out immediately after the h incubation period, and then and ... content (oxidized and reduced forms) was assayed in the cells immediately after incubation (6 h), and and h after reoxygenation (Fig 4) Under normoxia, intracellular reduced and oxidized glutathione...

Ngày tải lên: 18/02/2014, 16:20

... (1999) Hepatocarcinoma-intestine-pancreas/pancreatic associated protein (HIP/PAP) is expressed and secreted by proliferating ductules as well as by hepatocarcinoma and cholangiocarcinoma cells ... homogenates from control (Neo) and HIP/PAP-expressing cells were analyzed by Western blotting for HIP/PAP [13% (w/v) SDS/PAGE] and, for SERCA 2, RIIa and Ca subunits of PKA, and calreticulin [9% (w/v) ... subcloned at EcoRI and XhoI sites in pcDNA3.1, and in pcDNA3.1/myc-His (Invitrogen) The QuickChange Site-directed Mutagenesis Kit (Stratagene) was used to switch serines 73 and 138 and threonine 153...

Ngày tải lên: 23/03/2014, 13:20

... constructs (1S and 2S) and with c-met 3¢-UTR constructs (1S, 2S, 3S, and 4S) indicated that miR-23b can interact with the 1S and 2S target sites within the uPA 3¢-UTR, and with the 1S and 2S target ... h and 6.68 ± 1.11fold at 72 h (Fig 5A), and the corresponding enzymatic activity increased 3.1 ± 0.33-fold and 9.55 ± 2.12-fold, as compared with the control, at 48 and 72 h (Fig 5B, lanes and ... 0.27-fold and 2.84 ± 0.64-fold at 48 and 72 h (Fig 6B, lanes and 6, P < 0.05) As shown in Fig 6C, the detectable miR-23b amount decreased in transfected cells by 91% and 79%, respectively, at 48 and...

Ngày tải lên: 29/03/2014, 23:20

... of hepatocellular carcinoma Hepatology 6, 1100–1105 26 Ijzermans, J N M and Bac, D J (1997) Recent developments in screening, diagnosis and surgical treatment of hepatocellular carcinoma Scand ... (1993) Risk factors for hepatocellular carcinoma among patients with chronic liver disease N Engl J Med 328, 1797–1801 Manns, M P and Kubicka, S (1997) Hepatocellular carcinoma Digestion 58(Suppl ... K (1989) Epidemiology of hepatocellular carcinoma Gastroenterol Clin North Am 16, 545–551 Kew, M C and Popper, H (1984) Relationship between hepatocellular carcinoma and cirrhosis Semin Liver...

Ngày tải lên: 11/04/2014, 07:02

... 50, 100, 200 and 300 μmol/L) MTT and flow cytometry were used to detect inhibitory effects of RES on the growth of serumstimulated HepG2 cells As shown in Table and and Figure 1A and Figure 1B, ... immunofluorescence and immunohistochemistry CAV1 and topoisomerase-alpha protein expression in tissue and cells was studied by Immunofluorescence and Immunohistochemistry As shown in Figure 4B and Figure ... cis- and trans-isomers, and the trans-isomer has greater anticancer and cardio-protective properties than the cis-isomer As an estrogen analog activating ERα and ERβ, RES was suggested as a candidate...

Ngày tải lên: 18/06/2014, 15:20

... cell lung carcinoma [24] and cutaneous squamous cell carcinoma [25], but is decreased in colorectal carcinoma [26] and ovarian carcinoma [27] Table 3: The expression of p53, p21/WAF1, and MDM2 ... primary hepatocellular carcinoma: clinicopathologic correlation and survival analysis Hum Pathol 2001, 32:778-784 Schoniger-Hekele M, Hanel S, Wrba F, Muller C: Hepatocellular carcinoma survival and ... in carcinomas of the breast, ovary, and endometrium Am J Pathol 1997, 150:167-175 Qin LF, Ng IO, Fan ST, Ng M: p21/WAF1, p53 and PCNA expression and p53 mutation status in hepatocellular carcinoma...

Ngày tải lên: 18/06/2014, 15:20

... factors and surveillance of hepatocellular carcinoma Eur Rev Med Pharmacol Sci 14:352-355 Frau M, Biasi F, Feo F, Pascale RM: Prognostic markers and putative therapeutic targets for hepatocellular carcinoma ... the study design ML and RZL performed the experiments and draft the manuscript JWC, JBL YC, JHH and QLW participated in the data analysis and interpretation All authors read and approved the final ... patients with hepatocellular carcinoma be staged? Validation of a new prognostic system Cancer 2000, 89:2266-2273 30 Lau WY, Lai EC: Hepatocellular carcinoma: current management and recent advances...

Ngày tải lên: 18/06/2014, 16:20

... Figure and Load and genetic parameters in the two groups of patient (SVR and TF group) and at two time points (E, prior therapy Viral Load and genetic parameters in the two groups of patient (SVR and ... definitively understand the contribution of these naturally occurring variant viruses to the HCV quasispecies population dynamics and their implication in the HCV life cycle and pathogenicity Discussion ... pre-treatment HCV quasispecies diversity (QD) could potentially be used to identify responders and non-responders Currently there is little information on the temporal changes to the QD in chronic HCV...

Ngày tải lên: 20/06/2014, 01:20

... on HCV RNA and protein expression using primary productive HCV infection or persistent non-productive HCV infection in cultured replicon cells We report that ARB inhibits acute and chronic HCV ... Bio- In conclusion, ARB inhibits HCV acute and chronic HCV infection and replication The inhibitory effect appears to be due to the interaction of ARB with membranes and to subsequent ARB-induced ... negative for HCV RNA HCV pseudoparticle (HCVpp) assay The HCV pseusoparticle (HCVpp) system was described in details elsewhere [14,22] The measure of fusion between pseudoparticles and liposomes...

Ngày tải lên: 20/06/2014, 01:20

... tumors, adjunct methods in, 46–48 HCC vs metastatic adenocarcinoma/ cholangiocarcinoma, 45–46 histologic variants of HCC combined HCC and cholangiocarcinomas, 42–43 fibrolamellar HCC, 41–42 precursor ... and ultrasonography, 61 new serum markers and methods, 62 ultrasonography, 60–61 target population assessment of liver fibrosis, importance, 57 chronic HCV infection in Japan/Europe/US, study, ... 288 clinical trials and current evidence, 291–293 cisplatin/doxorubicin, 293 DNA intrastrand crosslinks, 293 lipiodol–cisplatin chemoembolization, 291–291 mitomycin C, 293 pre -and post-TACE, 291f...

Ngày tải lên: 07/07/2014, 09:20

... Hepatocellular Carcinoma Kelly M McMasters Jean-Nicolas Vauthey Editors Hepatocellular Carcinoma Targeted Therapy and Multidisciplinary Care 13 Editors Kelly ... (www.springer.com) Foreword It is a great pleasure and an honor to write the preface for this outstanding book dedicated to the therapy of hepatocellular carcinoma (HCC) This tumor, which is a major ... liver is a massive and complex organ requiring an excellent knowledge of its anatomy and physiology, with an exquisite comprehension of its impact on cardiovascular, pulmonary, and renal function...

Ngày tải lên: 07/07/2014, 09:20

... Epidemiology and Pathogenesis of Hepatocellular Carcinoma Manal M Hassan and Ahmed O Kaseb Biology of Hepatocellular Carcinoma Maria Luisa Balmer and Jean-François Dufour 21 Hepatocellular ... University of Texas MD Anderson Cancer Center, Houston, TX, USA Chapter Epidemiology and Pathogenesis of Hepatocellular Carcinoma Manal M Hassan and Ahmed O Kaseb Keywords Hepatocellular carcinoma · HCC ... Jean-Nicolas Vauthey, and Kelly M McMasters Evidence-Based Guidelines for Treatment of Hepatocellular Carcinoma in Japan Kiyoshi Hasegawa and Norihiro Kokudo Hepatocellular Carcinoma Arising...

Ngày tải lên: 07/07/2014, 09:20

... Epidemiology and natural history of hepatocellular carcinoma Best Pract Res Clin Gastroenterol 19:3–23 Epidemiology and Pathogenesis of Hepatocellular Carcinoma 13 El-Serag HB, Rudolph KL (2007) Hepatocellular ... cancer in American and European populations is likely to be associated with HCC development independent of chronic infection with HBV and HCV [120] Synergism between HBV /HCV and a family history ... Arachidonic acid Prostaglandin COX-2 Fig 1.2 Steps in hepatocarcinogenesis, modified from Xu et al [90] and Bensinger and Tontonoz [91] On the other hand, the association between obesity and HCC is hammered...

Ngày tải lên: 07/07/2014, 09:20