chitosan scaffolds for bone regeneration

... 45 s, annealing at 60 °C for 30 s, extension at 72 °C for min, Cloning of CrPrx cDNA and gene and a final extension at 72 °C for 10 The corresponding genomic sequence for CrPrx was PCR-amplified ... protein transformation vector The resulting plasmids were used to transform Agrobacterium tumefaciens strain GV3101 A standard leaf-disk transformation method [39] was used to generate transformants ... incubation on ⁄ 10 MS medium for various time peri1 · ThermoPol buffer, 1.5 mm MgCl2, 0.4 mm dNTPs, 0.2 lm each primer, and U of Deep VentR DNA Polymods before harvesting For each treatment, young...

Ngày tải lên: 23/03/2014, 09:21

... RCANACNACRTANACNCKRTGNACNSWNCCRTA for MJErev4 and GAYATGGCNGCNWSNGGNATH AAYCC for MJEfor3.1 With total RNA from cell suspension cultures and primer MJErev4 for first-strand synthesis, cDNA was ... must be a way to regulate the signal by controlled formation and – perhaps more importantly – by its controlled inactivation A candidate for performing the latter task is an esterase, previously ... homologous primers were designed for the rapid amplification of cDNA ends (RACE) (RACEfor: GTGA CAGCTTTCATGCCTGG; and RACErev: ATCCTGT CCGTTGTTGTAAAC) 5¢- and 3¢-RACE was performed using the SMART II...

Ngày tải lên: 16/03/2014, 18:20

... tissue responsible for the metabolism of metal ions and participates actively in the secretion of calcium and other ions for mineral growth in the process of the shell and pearl formation [37,38] ... induced by 0.5 mM IPTG for 2.5 h; lane 3, uninduced whole-cell lysate heated at 90 °C for 10 min; line 4, wholecell lysate induced by IPTG after heat treatment at 90 °C for 10 min; line 5, purified ... templates for the second round of PCR reaction The fragment named CaLP2 was amplified with a nested forward specific primer LS12 (5¢-CACAGACGGCAATGGA GAGG-3¢) and adaptor primer R2 The 5¢-RACE was performed...

Ngày tải lên: 16/03/2014, 23:20

... only for these plant processes but also for a comparison of multifunctional roles of AKRs in plants and mammals ACKNOWLEDGEMENTS The authors thank Dr Temp M Noshiro (Hiroshima University) for ... heat-shocked plants incubated at 41 °C for and h In all treatments the leaves were harvested and immediately immersed in liquid nitrogen Nucleic acid isolation and analysis For Northern analyses, 20 lg ... (10 000 g for 20 at °C) and the supernatant applied to a Ni–nitrilotriacetic acid spin column (Qiagen) equilibrated with buffer A The buffer B (buffer A with 50 mM imidazole) was used for washing...

Ngày tải lên: 18/03/2014, 01:20

... pPC97–p76RBE–HR1 or the complete p76RBE were transformed with the different Rho constructs in pPC86 For the screening, pJ69–4A harboring pPC97– p76RBE–HR1 was transformed with the dog thyroid library in ... packed volume of protein G-sepharose, at °C for h The extracts were centrifuged at 12 000 g for at °C and the supernatants were incubated with lg of 9E10 for h tumbling at °C, with a further h after ... (w/v) BSA for h They were then incubated with the primary antibody at a concentration of lgÆmL)1 for h at room temperature, and with protein A peroxidase (Sigma) at a : 10 000 dilution for h Detection...

Ngày tải lên: 23/03/2014, 21:20

... incubated at room temperature for minutes to allow nucleoproteins to dissociate before chloroform was added The mixture was shaken vigorously by hand for 15 seconds and incubated for another minutes This ... H2O and 17 ml of 37% formaldehyde) Each RNA sample contained 50% formamide, 1X MOPS, 7% formaldehyde and 0.1 mg/ml ethidium bromide; and was heated at 65°C for 10 minutes before loading with 1X ... EDTA, pH8.0; 10 ml of deionised formamide; 50 mg Dextran blue and 39 ml of H2O) and heated to 92°C for minutes Samples were then chilled on ice for minutes before being loaded into the wells...

Ngày tải lên: 03/10/2015, 20:57

... performed in triplicates Amplification conditions were 40 cycles with the following profile: 95 °C for 30 s, 60 °C for 30 s, and 72 °C for For each kind of tissue, standard curves were generated for ... melanogaster) formed a distinct cluster apart from the secreted (CAVI) and membrane-bound (CAIV) isoforms The present study could support the existence of a more ancient a-CA-like ancestor for both ... mass 5318 of each translated transcripts and found 26 973 Da for RpCAbr and 27 084 Da for RpCAtr The difference of almost kDa obtained for the trophosome CA protein FEBS Journal 274 (2007) 5311–5324...

Ngày tải lên: 18/02/2014, 16:20

... at 4000 g for 15 at 20 °C Additional supernatant was removed from the cell debris by ultra centrifugation at 60 000 g for 60 at °C The resulting extracellular fraction (EC) was used for the assay ... ethers: Lignin model substrates for the possible fluorometric assay of b-etherases Holzforschung 33, 134–135 16 Peizer, L.R & Vogel, H (1969) An autoclavable medium for the isolation of mycobacteria ... dimethylsulfoxide-d6) (Fig 2) For the Ca position reduction of GOU aO in DHP-GOU (Ca carbonyl type), g each of DHP-GOU and sodium borohydride were dissolved in dioxane/methanol (4 : 1) and stirred for 12 h at...

Ngày tải lên: 17/03/2014, 03:20

... v) formic acid (solvent C) and 0.1% (v ⁄ v) formic acid in 90% (v ⁄ v) CH3CN (solvent D), using a program of 5% (v ⁄ v) solvent D for min, a gradient of 1.05% per for 90 and 100% solvent D for ... 105) were grown on glass coverslips in 35 mm dishes for 48 h, fixed with 4% paraformaldehyde for 20 and treated with 0.1% (v ⁄ v) Triton X-100 for The fixed cells were incubated with affinity-purified ... treated with DNase I and RNase A (final concentration of 125 lgÆmL)1 for each enzyme) at °C for h and then centrifuged at 800 g for 15 The supernatant was designated the ‘chromatin fraction’ The...

Ngày tải lên: 30/03/2014, 20:20

... task, B1 dwelled at the character's location for 250 ms to select it; this was the method B1 regularly used for typing for more than seven years For the second task, B1 raised his eyebrow to ... 10 for cued response tests, was not fatiguing to use for prolonged periods, and required minimal effort to control These results suggest that MMG may be used as a noninvasive access pathway for ... typed a pangram for each of two selection modalities: dwell and eyebrow-raise For both typing tasks, B1 used the head-tracker to point to a character on an on-screen keyboard For the first task,...

Ngày tải lên: 19/06/2014, 08:20

... versus 2–12 cm2 for the other sensors and so the response time for the shoulderblade foam sensor would be slightly slower than that for the other sensor positions, e.g seconds for shoulder lift ... generally designed for use in stationary devices Consequently, the achievement of certain design goals for existing sensors (such as durability) is ultimately detrimental to the user's comfort when applied ... sensor placement exhibits a response of greater magnitude for extension than for flexion Since the sensor provides no additional qualitative information, it is hypothesized that a second sensor would...

Ngày tải lên: 19/06/2014, 10:20

... colleagues [5] performed a genetic complementation screening approach looking for stromal cellderived factors involved in cell proliferation and identified c19orf10 as a murine bone marrow stroma-derived ... paraformaldehyde (Polysciences, Inc., Warrington, PA, USA) in PBS for 15 minutes The cells were then washed and, in some cases, permeabilized using 0.2% Triton X-100 (Sigma-Aldrich) in PBS for ... control Paraffin blocks were prepared by immersing tissue samples in neutral buffered 10% formalin for hours for fixation They were dehydrated in ascending graded ethanol and infiltrated and embedded...

Ngày tải lên: 09/08/2014, 10:20

... (5'-3') CODhqtFor CODhqtRev HQT -For HQT-Rev HCT-ForRT HCT-RevRT HQT-ForRT HQT-RevRT ACT-ForRT ACT-RevRT HCT -For HCT-Rev HCT-InnerFor HCT-InnerRev HCT-OuterFor HCT-OuterRev HQT-InnerFor HQT-InnerRev ... previously used for map development [36] The full length HCT and HQT sequences were amplified on parental genome with sets of primers (one for each isolated gene, HCT -For, HCT-Rev, HQT -For and HQT-Rev ... induced a significant increase in transcription (12.3 ± 1.8 fold for HCT and 4.4 ± 0.7 fold for HQT) Comparison between the standard curves for each enzyme revealed a correlation coefficient of > 0.98...

Ngày tải lên: 12/08/2014, 03:20

... and for the absence of mutations The Saccharomyces cerevisiae strain L40 was then co-transformed with the recombinant plasmids using lithium acetate method and the transformants were analysed for ... abundant surface protein and responsible for the neuraminidase activity of the virus It is important both for its biological activity in Page of 12 (page number not for citation purposes) Virology Journal ... fitness of the virus [35]; therefore, the effect of peptide on the neuraminidase protein was assessed Unfortunately, this experiment showed a negative result for the fusion phages and cyclic...

Ngày tải lên: 12/08/2014, 04:21

... transformation procedures as for TAGs, we estimated the retrogene formation rate to be 0.176 to 0.228 × 10-3 per gene per MY in human and 0.393 to 0.642 × 10-3 per gene per MY in mouse for the ... than ten times slower than retrogene formation rates for twocopy families, but the contrast in rates of duplication for these two mechanisms becomes less obvious for the entire genome The rates estimated ... rates of duplication for the entire genome to be 0.076 to 0.325 × 10-3 per gene per MY in human and 0.202 to 1.45 × 10-3 per gene per MY in mouse Therefore, the rates estimated for the entire genome...

Ngày tải lên: 14/08/2014, 08:20

... subcutaneously for hydration and were placed in temperature controlled housing overnight for monitoring recovery All surgical and animal handling procedures were performed as approved under the Guide for ... provide impetus for making the good experiment “great” Lastly, I thank my research advisory committee for steering me towards the more important goals in graduate research training and for all the ... cytosol, resulting in the formation of the apoptosome Apoptosomes are responsible for activating caspases, including the well-known catalytic enzyme caspase 3, that are responsible for promoting apoptotic...

Ngày tải lên: 24/08/2014, 09:14

... to Chinese Pharmacopoeia (2000 edition), realgar-contained formulations (23) account for 5.67% of total TCM formulations They are used for psoriasis, syphilis, asthma, rheumatism, haemorrhoids, ... effective in treating tuberculosis and syphilis Arsphenamine was the standard therapy for syphilis for nearly 40 years before it was replaced by penicillin [Kasten FH, 1996] In fact, until the introduction ... treatment The CR rates for newly diagnosed patients are 72-85% and 85-93% for relapsed APL patients [Zhang TD et al., 2001; Chen Z at al., 2001] ATO is administered in the form of 1% solution at...

Ngày tải lên: 11/09/2015, 16:03

... tissue engineering……………… 17 Table Summary for previous RGD bearing biomaterials for hepatocytes culture……24 Table Summary for the galactosylated substrata for hepatocytes cultures………… 26 Table Diffusivity ... and CYP3 family are the ones involved in xenobiotics metabolisms (for example CYP1A2 for caffeine, CYP2C9 for ibuprofen, CYP3A4 for cocaine or acetaminophen) However, in some cases, CYPs also activates ... patient's lives before liver transplantation, establishing in vitro hepatocyte-based model 3) establishing culture model for drug metabolism/toxicity screening for drug discovery and 4) for basic researches...

Ngày tải lên: 12/09/2015, 08:19

... Ms Gong lingli for doing the echocardiography for the patients Thanks to Ms Connie Tse, for helping to collect the blood samples and the clinical data Thanks to Dr Sivalingam SP for his humor, ... Singapore for offering me the Research Scholarship and Singapore General Hospital for providing me technical and research support Finally, I would like to give the greatest gratitude to my family for ... at highest risk for SLE suggesting a key role for hormonal factors in SLE etiology In addition, a temporal increase in SLE burden has been reported by a number of researchers For instance, from...

Ngày tải lên: 14/09/2015, 12:42