chitosan based nanoparticles as delivery systems of therapeutic proteins

... formulation, DNase protection and release of pDNA on the in vivo efficiency of chitosan- based pDNA complexes after lung administration The efficiency is viewed as the product of the release of pDNA ... Trisaccharide-substituted chitosan oligomers as nonviral gene delivery systems (Paper II) The aim of Paper II was to investigate whether the gene delivery efficiency of polyplexes based on fully-deacetylated chitosan ... potential of chitosan to improve the transmucosal delivery of macromolecular drugs The positive effect of chitosan solutions/powders on the transmucosal delivery of peptides and proteins has been...

Ngày tải lên: 23/08/2015, 17:29

... scientiÞc support in most cases In fact, the story of nutraceutical beverages has not always been one based on solid science because of the indiscriminate addition of a host of nutraceuticals to water ... milk was often described as possibly nature’s most perfect food, its image was unfairly tarnished because of its natural abundance of cholesterol and saturated fat and the association of these ... potential source of dietary Þber has generated as much interest in the past to years as FOS Commonly referred to as a prebiotic,126 according to claims, a daily regime of to g of FOS per day is...

Ngày tải lên: 25/10/2013, 21:20

... on finding such a delivery system Applying novel delivery systems to RDO transfer Fig RDO delivery systems and transfer barriers decreased to 40% of normal, showing the effect of RDO conversion ... need evaluation Nanoparticles are new delivery systems By the method of associating with oligonucleotides [39], they can be classified into encapsulating nanoparticles, complexing nanoparticles ... Preparation of chitosan self- aggregates as a gene delivery system J Control Release 51, 213–220 37 Erbacher, P., Zou, S., Bettinger, T., Steffan, A.M & Remy, J.S (1998) Chitosan- based vector/DNA...

Ngày tải lên: 31/03/2014, 08:20

... use of large quantities of DNA As shown above, the encapsulation of antigen into PLGA microspheres allows the development of controlled-release delivery systems, in which the release profile of ... formulation was able to protect mice as well as guinea pigs against M tuberculosis as efficiently as three doses of naked DNAhsp65 There was a significant reduction in the number of bacterial ... amount of DNA was determined as described before using the Gene Quant II The protein encapsulation rate was determined after addition of 0.2 ml of acetonitrile The sample was incubated in an ultrasound...

Ngày tải lên: 14/08/2014, 19:22

... Influence of PCL mass ratio on shape and spreading of mouse embryonic fibroblasts on chitosan- PCL mixture membranes …………………………………………… 89 6.3 Influence of mixing chitosan and PCL on cellular activity of ... this class of materials include design of adhesion molecules for specific cell types and lack of materials with mechanical properties matching those of living tissues 2.4 CHITOSAN: Chitosan has been ... tunability of biomechanical and degradation properties, of chitosan is important, because of the wide range of properties exhibited by natural tissues For example, soft tissues such as skin has a strength...

Ngày tải lên: 13/11/2014, 09:32

... Page of 10 shown in Table 1, the size of free FA-PEAs was decreased obviously along with increase in its concentration When the concentration was higher than mg/ml, the size of FA-PEAs was stable ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) of 5, while ... size of PEI:pVHL complexes was larger than the FA-PEAs:pVHL complexes obviously at same ratio Generally, the particle size of FA-PEAs:pVHL complexes was decreased along with the increase of the...

Ngày tải lên: 18/06/2014, 19:20

... Page of 10 shown in Table 1, the size of free FA-PEAs was decreased obviously along with increase in its concentration When the concentration was higher than mg/ml, the size of FA-PEAs was stable ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) of 5, while ... size of PEI:pVHL complexes was larger than the FA-PEAs:pVHL complexes obviously at same ratio Generally, the particle size of FA-PEAs:pVHL complexes was decreased along with the increase of the...

Ngày tải lên: 20/06/2014, 03:20

... 3.3.4 pH-Dependent Swelling of P4VP -based Nanoparticles 102 3.3.5 pH-Swing Titrametric Swelling of P4VP -based Nanoparticles 105 3.3.6 Palladium-induced Deswelling of P4VP Nanoparticles 112 3.4 Conclusion ... 2.2.1 Iron -based Nanoparticles 13 2.2.1.1 Introduction 13 2.2.1.2 Pristine zero-valent iron (Fe0) nanoparticles 16 2.2.1.3 Modified iron -based nanoparticles 18 2.2.2 Surfactant -based Micelles ... compositions of various P4VP -based nanoparticles synthesized 90 Table 3.4 pH-dependent swelling of P4VP-PEGMA nanoparticles (prepared according to Run B-5 in Table 3.2) 104 Table 3.5 Deswelling of P4VP nanoparticles...

Ngày tải lên: 10/09/2015, 15:54

... application of SBA-15 in the delivery of the small molecule model drug of ibuprofen (IBU), with emphasis on the influence of the different functional methods on the adsorption and release of IBU Chapter ... tolerance of a variety of manufacturing methods Polymeric drug delivery systems under wide investigations are classified according to the mechanism controlling the release of the drug Some of the basic ... sustained release of the large protein model drug of bovine serum albumin (BSA) on SBA-15 Chapter covers the factors affecting stability of the released BSA in fabrication of drug delivery systems, ...

Ngày tải lên: 13/09/2015, 19:46

... manifested a tri-phasic burstlike profile that was absent in SBF SBF-soaked scaffolds showed sustained release of the growth factors and total release was not achieved, whereas total release was realized ... infiltration of cells and loading of proteins The protein release profile is a function of the degradation of the scaffolds The objective was to characterize the in vitro degradation behavior of PCL-TCP ... efficacy of the scaffolds as delivery systems for multiple growth factors, platelet-rich plasma (PRP) was used The buffers sufficed as important determinants of the release profiles obtained for transforming...

Ngày tải lên: 15/09/2015, 17:10

... properties of nanoparticle based drug delivery system for drug delivery across the blood brain barrier were discussed Table shows a list of ideal propertis of polymeric -based nanoparticles for drug delivery ... in drug delivery Table Ideal properties of polymeric -based nanoparticles for drug delivery across the BBB Table Size and size distribution of different nanoparticles Table Zeta potential of different ... desired in vitro release kinetics of the nanoparticles, and high cellular uptake of nanoparticles [68-71] In this study the 13 effects of all these surfactants on the feasibility of nanoparticles to...

Ngày tải lên: 26/11/2015, 22:48

... aqueous phase The particle size increased with increasing lipid concentration as well as decreasing surfactant concentration (Figure 2B and C) This is reasonable because higher viscosity level of the ... capacity as well as low-drug expulsion (Lin et al., 2007) In vitro drug release Figure shows the in vitro release of VRS from NLCs in medium pH 1.2 and 6.8 Drug release of VRS from NLCs was similar ... was located inside solid lipid, resulting in this increase Due to favorable PDI, 1% of liquid lipid was selected for further experiments The next step was to evaluate the effect of organic phase...

Ngày tải lên: 26/12/2016, 10:09

... exposure on MHC class I presentation levels in DCs, as well as the feasibility of using this antigen delivery system in DC -based cancer immunotherapy DCs were treated with ovalbumin (OVA) as a model ... utilizing ultrasound for antigen delivery in dendritic cell -based cancer immunotherapy Journal of Controlled Release 2009, 133:198-205 106 Klippstein R, Pozo D: Nanotechnology -based manipulation of dendritic ... electroporation systems Two clinical vaccine delivery systems have been designed by Cyto Pulse including DermaVax™ and Easy Vax™ Easy Vax™ primarily targets the epidermis layer of skin as used in mass-scale...

Ngày tải lên: 06/03/2014, 02:21

... Action of SR proteins as positive regulators (B) Action of hnRNP proteins as negative regulators Fig Recognition of a weak 3¢ss of the HIV precursors The upper panel shows that the binding of U2 ... the enzymatic activity of viral reverse transcriptase or of the protease involved in virus protein maturation The absence of cellular proteins resembling HIV integrase has also been exploited ... here for IDC16 derivatives strongly support further assessment of inhibitors of SR protein splicing factors as a new class of antiretroviral therapeutic agents Concluding remarks The various approaches...

Ngày tải lên: 06/03/2014, 09:22

... such as solubility and stability Chemical Aspects of Drug Delivery Systems Table Routes of administration and general classification of drug delivery systems ROUTES OF ADMINISTRATION DRUG DELIVERY ... time of onset of action or drug targeting issues Similarly, selection of drug delivery class is based on these and other numerous factors, as well as features related to the properties of the ... several problems of controlled release drug delivery systems, Chemical Aspects of Drug Delivery Systems 12 particularly the low availability of some drugs, short residence time, first-pass metabolism...

Ngày tải lên: 28/03/2014, 10:20

... inhibitor, as a result of its inhibitory effect on glucose hexokinase [16,20,21], it was suspected that the observed decrease in reporter activity was caused by a depletion of ATP in these cells As expected, ... GSL of the NCCIT cell was Gb3 (Fig 6B) After 2DG treatment, the level of Gb3 markedly decreased, which was followed by an accumulation of LacCer and the appearance of another GSL (Fig 6A, asterisk) ... treatment of Fabry disease with no associated adverse effects Although 2DG cannot be used directly as a drug for the treatment of Gb3-related diseases because of its intrinsic toxicity, modification of...

Ngày tải lên: 30/03/2014, 01:20

... DRUG DELIVERY Advantages:  avoid the first-pass effect of drug cleareance ORAL MUCOSAL ROUTE Classification:  Sublingual delivery  Buccal delivery  Local delivery Sublingual delivery Buccal delivery ... Society of Chemistry, American Chemical Society, Wiley InterScience journals… CONTENTS Generalities of drug delivery Liposomes as drug delivery systems Polymers and polymeric systems for drug delivery ... disease GLOBAL DRUG DELIVERY MARKET BY ADMINISTRATION MODE Nasal 2% Ocular 2% Injectable/ Implant 3% Transdermal 8% Inhalation 32% Oral 53% CLASSIFICATION OF DRUG DELIVERY SYSTEM By the way of delivery: ...

Ngày tải lên: 18/04/2014, 15:37

... frequently cited causes of the exotic behaviour of nanoparticles As a consequence of their peculiar properties, the synthesis of nanoparticles has opened the door to the production of many technological ... such as plastics, explosives, drugs, surfactants, metals, and metallic alloys However, in the past decades, a novel strategy based on the reduction of well-known substances to the state of nanoparticles ... those based on the preparation of colloidal solutions and the use of mesoporous materials or to their capability to allow: the mere synthesis of nanoparticles the spatial control of nanoparticles...

Ngày tải lên: 04/06/2014, 15:18

... application of nanodelivery systems in medicine Design of nanotechnology – based drug delivery Systems Nanoparticles can be used in targeted drug delivery at the site of disease to improve the uptake of ... increasing p53 and BAX action, increasing caspase-3 and caspase-8 activities, decreasing Akt activity, and increasing expression of TNF-α [47] Our research group is investigating the interaction of ... presence of fluorescent QDs in the chitosan nanoparticles Targeted delivery of HER2 siRNA to HER2-overexpressing SKBR3 breast cancer cells has been specific with chitosan/ quantum dot nanoparticles...

Ngày tải lên: 20/06/2014, 00:20