chitosan as a molecular scaffold for biomimetic design of glycopolymer biomaterials

... evidence that LMP1 can act as a potent molecular adjuvant, providing a new class of adjuvant for use in recombinant vaccine strategies In addition, LMP1 and LMP1 chimeras could be used as viral vector ... CAG CCC TCG CTG TCA TCC-3 (forward) and 5-CAT CTC CAA AGA GTT GAT GTA CTC C-3 (reverse) For normalization, GAPDH and b-actin real-time PCR was carried out on the same samples Normalized mRNA ... Statistics Data were analyzed using PRISM 4.0 (GraphPad Software, La Jolla, CA) and expressed as the mean ± SEM Statistical comparisons were analyzed by Student’s t test A p-value of 0.05 was...

Ngày tải lên: 13/08/2014, 01:20

... hyperphosphorylation occurs because of an imbalance in the activity of tau kinases and phosphatases [3] One particular tau kinase pertinent to metal dyshomeostasis in AD is glycogen synthase kinase-3 ... Modulation of metal availability for treating AD P J Crouch et al research attention as potential therapeutic targets Plaques and NFTs, however, cannot be regarded as ‘upstream’ causative factors ... decreased availability of intracellular Cu [83] and up-regulated by increased availability of Cu [84] Collectively, these data present a strong case for the native role of APP ⁄ Ab in regulating...

Ngày tải lên: 07/03/2014, 10:20

... Cyanobacteria Synechocystis sp PCC6803 Rhodophyceae (red algae) Cyanidioschyzon merolae Nuclear DNA Chloroplast DNA Cyanidium caldarium Chloroplast DNA Bacillariophyceae (diatoms) Thalassiosira pseudonana ... pseudonana Nuclear DNA Chloroplast DNA Odontella sinensis Chloroplast DNA Prasinophyceae Mesostigma viride Chloroplast DNA Euglenophyceae Euglena gracilis Chloroplast DNA Chlorophyceae (green algae) ... apparent molecular mass of which was remarkably higher than that of PsbQ¢ (lane 4) in the red algal PSII In addition, this polypeptide band did not disappear by m alkaline Tris-treatment (data not...

Ngày tải lên: 23/03/2014, 15:21

... type diabetes subjects Immunogenetics 2010, 62:101-107 36 Kawasaki E, Awata T, Ikegami H, Kobayashi T, Maruyama T, Nakanishi K, Shimada A, Uga M, Kurihara S, Kawabata Y, et al: Genetic association ... Pediatr Res 2000, 48:6-11 19 Bernasconi A, Marino R, Ribas A, Rossi J, Ciaccio M, Oleastro M, Ornani A, Paz R, Rivarola MA, Zelazko M, Belgorosky A: Characterization of immunodeficiency in a patient ... Cobbold S, Alyanakian MA, Gouarin C, Barriot S, Garcia C, Waldmann H, Bach JF, Chatenoud L: Autoimmune diabetes onset results from qualitative rather than quantitative age-dependent changes in pathogenic...

Ngày tải lên: 18/06/2014, 16:20

... cesarean: a case series and review of the literature Am J Perinatol 2009, 26(10):739-744 Kurdoglu M, Kolusari A, Yildizhan R, Adali E, Sahin HG: Delayed diagnosis of an atypical rupture of an ... Cite this article as: Kuwata et al.: Intestinal adhesion due to previous uterine surgery as a risk factor for delayed diagnosis of uterine rupture: a case report Journal of Medical Case Reports ... References Gupta A, Nanda S: Uterine rupture in pregnancy: a five-year study Arch Gynecol Obstet 2011, 283(3):437-441 Morimatsu Y, Matsubara S, Higashiyama N, Kuwata T, Ohkuchi A, Izumi A, Shibhara H,...

Ngày tải lên: 10/08/2014, 23:20

... 5'ATCCAAGACGGAATTCACGCCGCAGGAGAAAGAAGCTATAG-3'; the 3' primer for the APOPEC3G-UBA2 fusion was 5'-ATCGTACTCGAAGCTTCTAACTCAGGAGGAAGTTGGCAG-3'; and the 3' primer for the APOPEC3G-UBA2* fusion was 5'-ATCGTACTCGAAGCTTCTAACTCAGagcGAAGTTGGCAG-3' ... used for the U and M plasmid was 5'-ATCCAAGACGGAATTCGTTTTCCTGATTCTGGAG-3' The UBA2 gene fragment was amplified from plasmid pcDNA3.1HHR2 3A by PCR The 5' primer used was 5'ATCCAAGACGGAATTCACGCCGCAGGAGAAAGAAGCTATAG-3'; ... construction of all three plasmids was 5'-GCGCGCGCGCCTCGAGACCATGAAGCCTCACTT-3'; The 3' primers used for the construction of the E plasmid was 5'-ATCCAAGACGGAATTCCTAGAACTCGTTTTCCTGATTCTGGAG-3' and the...

Ngày tải lên: 13/08/2014, 05:21

... a specific case of projects in CARE International in Vietnam Another reason is that the author has been a part of the “natural setting” She has been working in CARE International in Vietnam for ... consent for participation in the research The second and more important reason was that sending the questionnaire via email was fast, cheap and easy to reach the target groups and get feed back from ... University of East Anglia, pp.45-61 Australian National Training Authority, (2003), What makes for good workplace learning, National Centre for Vocational Education Research Ltd Baloto, F (1996),...

Ngày tải lên: 04/08/2015, 09:42

... 0) and condensation ( m phase < 0) is assumed Where m phase is mass transfer: for evaporation & & & & ( m phase = mevap ) and for condensation ( m phase = mcond ) (kg/s) So that the mass balance ... both gas and liquid phase in the same computational domain, and thus allows for the implementation of phase change inside the gas diffusion layers The model includes the transport of gaseous ... performance and design of air-breathing PEM fuel cells Litster and Djilali [1] developed a single-phase one-dimensional semi-analytical model of the membrane electrode assembly (MEA) of planar air-breathing...

Ngày tải lên: 05/09/2013, 14:58

... TGTAAAG TGTAAAG + ) + )95 )1100 )1470 ACACacG ACACttG ACACaaG + + + + ) ) ) ) ) )140 )1100 )1596 )1632 )140 )1100 )1596 )1632 )1874 CAaaTG CAagTG CAaaTG CAaaTG CAaaTG CActTG CAttTG CAgtTG TGAGTCA ... peptide was constructed as follows The DNA fragment was amplified from GmPDIM cDNA by PCR using the primers 5¢-GACGACGACAAGATGC ACGCACTCTATGGAGC-3¢ and 5¢-GAGGAGAAGC CCGGTTCATAGCTCATCCTTGCTTGAAG-3¢ ... RT-PCR Each value was standardized by dividing the value by that for actin mRNA Values are calculated as a percentage of the highest value obtained during maturation Data represent the mean ± SD for...

Ngày tải lên: 30/03/2014, 04:20

... quinquefasciatus quinquefasciatus quinquefasciatus quinquefasciatus quinquefasciatus quinquefasciatus Identity strain strain strain strain strain strain strain strain strain strain strain JHB ... effectiveness of DDT immunoconjugates is debatable In our opinion, in view of the devastating impact of diseases such as malaria on individuals and nations within malaria endemic areas, if such nanoparticles ... three RST (AJ970181, AJ970201 and AJ970301) as queries against the 1,778 organismal and the human genome databases by way of BLAST-N calibrated at Expect (E) = 10, Filtration (F) at Default, Description...

Ngày tải lên: 13/08/2014, 16:20

... And such a whole suite of accessory enzymes is required for efficient xylan hydrolysis such as α-arabinofuranosidases and α-Larabinases that release arabinan [31], α-glucuronidases that release ... feasible as great amounts of this valuable commodity would be wasted Besides lignin, miscellaneous components of wheat straw such as wax, pectin, and phenolic acids are also of great value and ... precipitate and separate the fractionated biomass The pretreatment has an advantage of operating at low temperature (50 °C) which capital and operating costs and minimizes degradation reactions The...

Ngày tải lên: 05/09/2013, 15:28

... 5¢-CACACTACACTGGGAAGCAGAGACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG3¢ The cDNA was subcloned into the EcoRV site of pBluescript SK(+) (Stratagene, La Jolla, CA, USA) and sequenced using an automated ... buffer, and the eluted samples were subjected to western blot analysis as described above The authors thank Drs Masato Yasui, Sadakazu Aiso and Masaaki Matsuoka for support; Dr Andrew P McMahon for ... Na-palmitoylation of Gas is regulated also remains unclear However, we assume that mammalian Gup1 competes with Skn for Shh to prevent palmitoylation rather than catalyzing depalmitoylation of...

Ngày tải lên: 18/02/2014, 16:20

... Jenco JM, Nakashima S, Cadwallader K, Gu Q, Cook S, Nozawa Y, Prestwich GD, Frohman MA & Morris AJ (1997) Characterization of two alternately spliced forms of phospholipase D1 Activation of the purified ... Valencia, CA, USA), and rocked at °C for h The lysate ⁄ Ni ⁄ nitrilotriacetate bead mixture was transferred to a poly prep chromatography column and the agarose packed The column was washed twice ... mm NaCl, and · protease inhibitor cocktail at 37 °C for 20 The lysate was spun down at 50 000 g for 15 The supernatant was mixed with pre-equilibrated anti-HA affinity matrix and rocked at °C for...

Ngày tải lên: 20/02/2014, 01:20

... lipid was extracted The effect of drying temperature was investigated in this study Gas chromatography analysis Sample was dissolved in ethyl acetate and 0.5 µL of this was injected into a Shimadzu ... that increasing CO2 concentration of up to 1% of air will increase lipid produced by algae was given in Table As shown in this table, cell concentration obtained after 20 days incubation was ... per unit area of land, compared to terrestrial oilseed crops Microalgae are very efficient biomass capable of taking a waste (zero energy) form of carbon (CO2) and converting it into a high density...

Ngày tải lên: 21/02/2014, 10:20

... lipid was extracted The effect of drying temperature was investigated in this study Gas chromatography analysis Sample was dissolved in ethyl acetate and 0.5 µL of this was injected into a Shimadzu ... that increasing CO2 concentration of up to 1% of air will increase lipid produced by algae was given in Table As shown in this table, cell concentration obtained after 20 days incubation was ... per unit area of land, compared to terrestrial oilseed crops Microalgae are very efficient biomass capable of taking a waste (zero energy) form of carbon (CO2) and converting it into a high density...

Ngày tải lên: 21/02/2014, 10:20

... regions has also suggested that NF-jB might be regarded as a signal transducer that transmits transient synaptic signals to the nucleus and has a role in behaviour, learning and memory formation ... Benetti A, Benarese M & Spano PF (2005) Inhibition of IkappaBalpha phosphorylation prevents glutamate-induced NF-kappaB activation and neuronal cell death Acta Neurochirurgica Suppl 93, 59–63 Aleyasin ... kinase A ⁄ CREB signalling Mol Cell Biol 26, 2936–2946 Kassed CA, Willing AE, Garbuzova-Davis S, Sanberg PR & Pennypacker KR (2002) Lack of NF-kappaB p50 exacerbates degeneration of hippocampal...

Ngày tải lên: 07/03/2014, 03:20

... phosphate as a substrate for localization of the labeled MYP Statistical analysis Data were expressed as the mean ± SEM Statistical analysis was performed using instat software (GraphPad Software) The ... testis at stage 1, and eggs In all of the samples analyzed, a large protein peak was observed at an elution position of 72 mL (peaks a, b, c, and d), where the estimated molecular mass was about ... measured by the Bradford method [51] using a Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA) with bovine c-globulin as a standard Thyroglobulin (669 kDa), catalase (232 kDa), BSA (67 kDa) and...

Ngày tải lên: 16/03/2014, 05:20

... structure of the Fab was fixed except for v angles of serine residues The search ˚ area was set within 10 A of the centroid of heavy atoms of GA4 in the crystal structure Other calculation parameters ... peptide-antibody recognition 11 Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T & Yamaguchi I (2002) Crystal structure of the liganded anti-gibberellin A4 antibody 4-B8(8) ⁄ E9 Fab fragment ... restraints, and eight dihedral angle restraints Fifty conformations that give low conformation energy and that give no distance and dihedral angle violations greater than ˚ ˚ 0.5 A and A, respectively,...

Ngày tải lên: 16/03/2014, 23:20

... OF DATR DATR (described in detail by Evans/ Gazdar, 198 9a; 1989b; 1990)is a declarative language for the definition of semantic networks which allows for defaults as well as multiple inheritance ... the information about the bar level can be queried separately As a declarative language, DATR is independent of the procedural evaluation strategies embodied in particular DATR-implementations ... commas, and the square brackets are DATR atoms, not part of the languageitself.In contrast, the parentheses of DATR enclose a list value Shieber, Smart M (1986) An Introduction to Unification-Based...

Ngày tải lên: 18/03/2014, 02:20