chitin chitosan oligosaccharides effective substrates for functional analysis of chitinases chitosanases

... generated by software programs available at the EXPASY proteomics server of the Swiss Institute of Bioinformatics (http://www.expasy.org) The nucleotide alignment of peroxidases for making the ... roseus (L) G Don and the biosynthesis of a-3¢, 4¢-anhydrovinblastine: a specific role for a multifunctional enzyme Protoplasma 222, 97–105 Bioinformatics analysis of CrPrx Hiraga S, Sasaki K, Ito H, ... method for Identification of a basic glycoprotein induced by ethythe quantitation of microgram quantities of protein utillene in primary leaves of azuki bean as a cationic perizing the principle of...

Ngày tải lên: 23/03/2014, 09:21

... been speculated that the physical role of MeJA is to mobilize JA [19] The proof of this hypothesis might come from a more detailed understanding of how plants form MeJA from JA, and vice versa A ... less than expected for an enzyme from heterologous bacterial expression of the cDNA For visualization of proteins, bacterial extracts were subjected to SDS/PAGE In a comparison of MJE-producing ... purification of MJE there was no evidence for the expression of isoenzymes, although, if present, they might have different catalytic properties Northern blot analysis and induction of MJE expression...

Ngày tải lên: 16/03/2014, 18:20

... tissue responsible for the metabolism of metal ions and participates actively in the secretion of calcium and other ions for mineral growth in the process of the shell and pearl formation [37,38] ... The shells of oyster consist of 90% CaCO3, products of calcium metabolism, and a few percent of matrix of biological macromolecules This highly controlled process may depend on presence of different ... in EF-hand domains of oyster CaLP may contribute significantly to its specific selectivity for substrates and physiological function Comparison of the aminoacid composition of the calcium-binding...

Ngày tải lên: 16/03/2014, 23:20

... with stress tolerance This knowledge would be of great importance not only for these plant processes but also for a comparison of multifunctional roles of AKRs in plants and mammals ACKNOWLEDGEMENTS ... were grown in a greenhouse for months For mechanical wounding experiments, holes of mm diameter were made across the lamina, which effectively damaged approximately 5% of the leaf area Samples ... both mono- and di-cotyledonous plants An analysis of the primary structure of DpAR1 and DpAR2 showed three consensus patterns specific for this family of proteins One is located in the N-terminus...

Ngày tải lên: 18/03/2014, 01:20

... Genome Browser) Specific association of p76RBE with GTP-bound form of RhoB The presence of an HR1 domain and the high degree of homology with Rhophilin in the NH2 part of the protein suggested that ... RhoCQ14L cDNA were obtained by quickchange punctual mutations of the wt cDNA of RhoC in pPC86 (kind gift of P Chevray of the University of Texas, Houston, TX, USA and D Nathans from the Howard Hughes ... with 20 lL packed volume of protein G-sepharose, at °C for h The extracts were centrifuged at 12 000 g for at °C and the supernatants were incubated with lg of 9E10 for h tumbling at °C, with...

Ngày tải lên: 23/03/2014, 21:20

... contains ml of 25 mM EDTA, pH8.0; 10 ml of deionised formamide; 50 mg Dextran blue and 39 ml of H2O) and heated to 92°C for minutes Samples were then chilled on ice for minutes before being loaded ... denaturing agarose gel (100 ml of gel contains 1.2 g agarose, 10 ml of 10X MOPS, 73 ml of H2O and 17 ml of 37% formaldehyde) Each RNA sample contained 50% formamide, 1X MOPS, 7% formaldehyde and 0.1 mg/ml ... Accumulation of klhl Tissue Distribution Analysis of klhl in Adult Zebrafish Expression of klhl is Similar in Human, Rat and Zebrafish Ontogenetic Expression of klhl during Somitogenesis 10 Expression of...

Ngày tải lên: 03/10/2015, 20:57

... Further functional and histological studies suggested the existence of several carbonic anhydrase isoforms in the trophosome tissue [16,17], indicating the possible existence of various CA isoforms ... performed in triplicates Amplification conditions were 40 cycles with the following profile: 95 °C for 30 s, 60 °C for 30 s, and 72 °C for For each kind of tissue, standard curves were generated for ... was composed of lL of Moloney murine leukemia virus reverse transcriptase buffer; 0.5 lL of BSA (10 mgÆmL)1), lL of total RNA (1.24 lgÆlL)1), 2.5 lL of dNTP (4 mm total), 1.5 lL of Random Primer...

Ngày tải lên: 18/02/2014, 16:20

... a Enzymic reaction and analysis of metabolites Reactions were initiated by the addition of 100 ll of a solution of enzyme (200 lgÆmL)1) to 0.9 mL of a solution of 50 lg of substrate in 10% (v/v) ... of 0.1 g of HP or M to 1.0 mL of VM containing 10 lg of GOU After incubation for 12 h at 28 °C, the reaction mixture was centrifuged at 15 000 g for 10 Reactions were terminated by addition of ... (C) 1H-NMR spectrum of acetylated DHP-GOU (Ca alcohol type) For EC and CY, reactions were initiated by addition of 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions...

Ngày tải lên: 17/03/2014, 03:20

... v) formic acid (solvent C) and 0.1% (v ⁄ v) formic acid in 90% (v ⁄ v) CH3CN (solvent D), using a program of 5% (v ⁄ v) solvent D for min, a gradient of 1.05% per for 90 and 100% solvent D for ... accordance with the guidelines of the Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation Subcellular fractions of rat liver were prepared ... Ministry of Education, Science, Sports and Culture of Japan and grants from the Biodesign Research Project of RIKEN and for Project Research of Niigata University 4336 Transient expression of GFP-ISP36...

Ngày tải lên: 30/03/2014, 20:20

... CWT analysis was performed on the MMG signal, xmmg, using non-overlapping sliding windows, 100 ms in length The output of CWT analysis is a muscle activity event, z [k], for each sample, k, of ... the frontal belly of the occipitofrontalis muscle of the forehead with an elastic strap, as shown in Figure The sensor was placed cm above the eyebrow, above the inside corner of the right eye ... practiced using the switch for hour, perform- ing activities such as typing using a scanning keyboard At the end of the hour, participants were asked to rate the level of effort and fatigue associated...

Ngày tải lên: 19/06/2014, 08:20

... peri-personal space One of the most compelling needs for wearable technology is in the continuous monitoring of the human body, be that for medical monitoring or to inform the operation of a context-aware ... courtesy of the University of Bath, England, showed that the stress-strain profile of the unadulterated PU foam sample and that of the PPy-coated PU foams sample were similar showing regions of elastic ... resistance and range of the measured resistance of the sensors as determined through a series of standard repeatable exercises by the Page of (page number not for citation purposes) Journal of NeuroEngineering...

Ngày tải lên: 19/06/2014, 10:20

... with a ProFound expectation score of 2.5 × 10-6 [11] (Figure 1a) Subsequent MS/MS analysis of three of the peptides derived from this spot confirmed that the sequence corresponded to that of c19orf10 ... Dasuri for the initial 2DE gel that resulted in the discovery of c19orf10 We acknowledge the technical assistance of Patty Sauder for production of the murine monoclonal antibodies, Miranda Ma for ... limited intermittent staining of a subset of cells In addition, areas of synovial hyperplasia show variable staining patterns In some cases, areas of hyperplasia are positive for c19orf10 staining (Figure...

Ngày tải lên: 09/08/2014, 10:20

... (5'-3') CODhqtFor CODhqtRev HQT -For HQT-Rev HCT-ForRT HCT-RevRT HQT-ForRT HQT-RevRT ACT-ForRT ACT-RevRT HCT -For HCT-Rev HCT-InnerFor HCT-InnerRev HCT-OuterFor HCT-OuterRev HQT-InnerFor HQT-InnerRev ... basis of our data this might be a consequence of the rapid conversion of CGA to DCQs as by means of an unknown downstream enzymatic step Indeed the involvement of the HQT gene in the profile of ... [33,34] The presence of allelic variation at the sequence level in genes of known biochemical functional is useful for candidate Isolation and cloning of a full length HQT cDNA of globe artichoke...

Ngày tải lên: 12/08/2014, 03:20

... cyclic form; L-P1 – Linear form; FP-P1 – fusion phage displaying this peptide) Estimation of binding abilities of selected phage clones Recombinant phages selected from the fourth round of panning ... pAb for binding sites on AIV In the presence of the antibody, the number of phages bound to the AIV coated wells reduced dramatically as a result of the competition between these two molecules for ... (Miller Units) of independent colonies for each co-transformation (The SD value is not shown as there was very little variation between repeated experiment) Figure blot analysis of immunoprecipitated...

Ngày tải lên: 12/08/2014, 04:21

... the cut-off and fitted linear models to each part of the curves The results are shown in Table The slopes of the linear functions are therefore the estimates of gene duplication rates for the ... Ks cut-off for the slowdown of the observed duplicated gene formation rates in TAG (about 0.25) is much larger than that of retrogenes (about 0.05) This phenomenon is largely because of the influence ... Similarly, we also plotted the distribution of cumulative number of retrogenes as a function of Ks using R [46] The interval of the data points in terms of Ks of the curves is 0.01 http://genomebiology.com/2007/8/8/R158...

Ngày tải lên: 14/08/2014, 08:20

... activity of the mammalian target of rapamycin (mTOR) is a major inhibitor of the progression of apoptosis, and stability of mitochondrial function may result from PINK-1-induced upregulation of Akt ... transplantation strategy for experimental and clinical treatment of SCI Limitations and methods of improving SC transplantation for SCI In spite of the many advantages of SC transplantation, there ... targets for pharmacological therapeutics Table highlights some of the most commonly used chemical inhibitors, their targets, and functions for assessing the roles of particular steps of these...

Ngày tải lên: 24/08/2014, 09:14

... is extremely limited information of these arsenicals in terms of the mechanisms of action, toxicity, as well as pharmacokinetic and pharmacodynamic profiles The lack of information could be caused ... 2006] Inhibition of growth and promotion of apoptosis Observations of the clinical utility of realgar in the treatment of APL have triggered investigations into the mechanisms of action by which ... chromatograms………………………………………………………….166 6.3.2 Characteristics of SPE LC/MS/MS method for quantification of urinary 8OH-dGuo……………………………………………………………….175 6.3.3 Concentrations of 8-OH-dGuo in rats urines before and after arsenic compounds...

Ngày tải lên: 11/09/2015, 16:03

... basis of structural and functional features Only about seven of the families are important for drug metabolism The cytochrome P450 3A group (CYP3A) is responsible for metabolism of over 50% of the ... detoxification of xenobiotics Among the CYP families, CYP1, CYP2 and CYP3 family are the ones involved in xenobiotics metabolisms (for example CYP1A2 for caffeine, CYP2C9 for ibuprofen, CYP3A4 for cocaine ... Toxicology strategies are important screening step before clinical trials of new drug candidates [18] -8- related forms of cytochrome P450 enzymes (isoforms, or isoenzymes) have been characterized which...

Ngày tải lên: 12/09/2015, 08:19

... A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like ... Associate Professor Koh Dow Rhoon, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, for his guidance I am also very grateful to Dr Tin Soe Kyaw for his ... supervisor Associate Professor Fong Kok Yong, Chairman of Division of Medicine, senior consultant, Department of Rheumatology and Immunology in Singapore General Hospital, for his invaluable supervision,...

Ngày tải lên: 14/09/2015, 12:42