chip and real time pcr analysis of chip dna

... reduction of infectivity of TZM-bl cells was measured as a function of the degree of IgG1b12 mediated neutralization of these Envs association of autologous neutralization sensitivity of patient ... 11, IVC and and IVC and 11 their sensitivity to sCD4 and contemporaneous plasma antibodies Thus, Envs 2-3.J18, 2-5.J3 and 2-5.J11 which showed the highest neutralization sensitivity (IC50 of 0.5, ... dependence of patient Envs with their sensitivities to autologous plasma antibodies and sCD4 Association of CD4 usage of Env-pseudotyped viruses with autologous plasma antibodies and (P < 0.0155) and...

Ngày tải lên: 13/08/2014, 01:20

... photographed after days of growth at 30 °C on YPDA in the absence or presence of the indicated concentrations of GA or RD (B) RD and GA sensitivities of strains bearing deletions of the Pdr5 and Snq2 plasma ... by the loss of several of the cochaperones that are nonessential for viability of yeast (Sti1p, Sba1p, Cpr6p, Cpr7p, Hch1p and Aha1p) Sse1p and essential cochaperones such as Cdc37p and Cns1p were ... hsp82 and A587T hsp82 cells (Fig 3) Heat Fig Expression of a HSE-LacZ reporter of HSF activity in cells expressing the wild-type (p82a), or T101I and A587T mutant forms of Hsp82 Basal and heat-induced...

Ngày tải lên: 07/03/2014, 21:20

... capable of generating arsine in the presence of borohydride The arsenic content in rice and chicken is of the same order of magnitude and about three and two orders of magnitude lower than that of ... 10 ml of concentrated HNO3 were added and the reactor was covered and pre-digested overnight Next, 20 mg of Na2 S2 O8 and ml of HClO4 (or 0.3 ml of concentrated HF in rice) were added and heated ... powder candidate reference materials (prepared within the framework of a European project) of environmental and biological origin: rice, chicken, fish and soil samples Sample preparation of lyophilized...

Ngày tải lên: 15/03/2014, 23:49

... of human ESR1 3¢-UTR) in human and other mammalian species, including the chimpanzee, rhesus monkey, mouse, rat, dog and rabbit (Fig 2A) The predicted DG of 70 bp 5¢- and 3¢-flanking regions of ... luciferase activity of pGL3m–ESR1–3¢-UTR–WT (ESR1–3¢-UTR–WT) and pGL3m–ESR1–3¢-UTR–MUT (ESR1–3¢-UTR–MUT) in HEK293T and MCF-7 cells cotransfected with pcDNA-3.0–miR-22 or pcDNA-3.0 and synthetic miR-22 ... is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate the maximum and minimum values The solid...

Ngày tải lên: 22/03/2014, 21:20

... Surviving clones were then expanded in the same medium and analyzed for the expression of TRPM4b by RT -PCR and western blotting RT -PCR Total RNA was isolated, and RT -PCR was performed as described ... Quantitative analysis of the depolarization of the plasma membrane 200 s after addition of ionomycin The results of a western blot experiment showing the different levels of expression of TRPM4b ... untreated (dashed lines) (B) Quantitative analysis of the amplitude of Ca2+ released by ionomycin (left) and the amplitude of Ca2+ entry after readdition of Ca2+ (right) Values for the Ca2+ influx...

Ngày tải lên: 23/03/2014, 09:21

... and limit of quantification of ofloxacin in plasma was found to be 99.2% and 0.01 mg/L respectively The extraction recovery and the limit of quantification for all the tissue was near 100 % and ... of 1st Annual Conference of Indian Society of Veterinary Pharmacology and Toxicology and National Symposium on newer perspective of Veterinary Pharmacology and Toxicology in the improvement of ... sheep [16].The analysis of the semi-logarithmic plasma-ofloxacin time profile curve revealed that it could best be analyzed by noncompartmental model More and more investigators and clinicians...

Ngày tải lên: 07/08/2014, 17:22

... Ministry of Public Health, Thailand, Table summarizes subject characteristics and laboratory parameters There were 40 patients with 70% male and a mean age of 37 years The frequencies of Page of (page ... design of the study, statistical analysis and draft the manuscript SK, WA, WP, ST, KR and SS participated in the design of the study and draft the manuscript SN participated in the design of the ... who had different stratum of plasma HIV-1 RNA at weeks 48 of treatment by intendto-treat analysis was displayed in figure By intend-totreat analysis, 33 (83%), 30 (75%) and 24 (60%) patients achieved...

Ngày tải lên: 10/08/2014, 05:21

... were collected and analyzed for the detection of HAV genome [9] Sequence analysis of HAV RNA genomes Sequence analysis of the nested PCR products of 5’NTR region, showed high degree of identity ... (environmental and clinical) by employing previously validated primer sets [9] Sequencing and analysis of viral genomes All positive samples (environmental and clinical) were confirmed by sequencing of the ... 8%) collected from the inlet of the biological treatment plant of Patras and in one sample (1/50, 2%) collected from the entry-point of the treatment plant of Alexandroupolis Sera from eight hospitalized...

Ngày tải lên: 12/08/2014, 01:21

... 50% of a group of test animals Mucus membrane and eye toxicity test: The conjunctiva of the eye and the vaginal vault of experimental animals (rabbits and monkeys) have been employed in tests of ... Tecnología Agraria y Alimentaria is the analysis of pesticides and other contaminants in food and environmental matrices and the evaluation of exposure to biocides and existing chemicals He has published ... leaves It is used for the control of storage roots of fruits and vegetables and it is effective for seed dressing of cereals Table 1.22 summarizes the properties of various frequently used fungicides...

Ngày tải lên: 12/08/2014, 04:22

... risk of exposure of the chemist to toxic solvents and vapors always exists From a practical point of view, the formation of emulsions, which are sometimes difficult to break up, the handling of ... use of solvent mixtures (especially for the extraction of pesticides of different polarity) combining the ability of heating of one of the components (i.e., acetone) with the solubility of the ... liquids The efficiency of the process depends on the affinity of the analyte for the solvents, on the ratio of volumes of each phase, and on the number of successive extractions Most of the LLE applications...

Ngày tải lên: 12/08/2014, 04:22

... using the latest concepts and techniques, better understanding and control of the combination of hapten molecules and macromolecular carriers, and improving the efficiency of ß 2007 by Taylor & Francis ... detection of organophosphate pesticides and nerve agents Anal Chem., 78, 835–843, 2006 78 Minunni, M and Mascini, M Detection of pesticide in drinking water using real- time biospecific interaction analysis ... determined and documented Triplicate analyses of each standard, control, and sample are generally performed for 96-microwell plate assays The %RSD of measured concentrations from triplicate analysis...

Ngày tải lên: 12/08/2014, 04:22

... frequency (time or distance), spacing, mixing, dividing of samples, and consequently the time required for sampling and the cost of sampling, shipping, and analysis of samples Careful balancing of cost ... safety, and security precautions Determination of the time required for reaching the sampling site and handling the samples Provisions for prevention of contamination and deterioration of samples ... residue and the average CV of the residues For instance, even if 30 pairs of random composite samples of size 10 were withdrawn 100 times from a data population having a CV of 0.28, the minimum and...

Ngày tải lên: 12/08/2014, 04:22

... cartridges and methanol as eluting solvent 8.2.3.3 Multiresidue Analysis of complex mixtures of pesticides in soil is a difficult problem because of the presence of a wide variety of compounds ... optimization for multiresidue analysis of pesticides in soil because of the disparity of chemical compounds involved Automation of sample preparation and coupling with instrumental analysis are also important ... simultaneous analysis of 2,4-D, dicamba, and mecoprop residues in soil wheat and barley, J Assoc Off Anal Chem., 58, 1027, 1975 Sánchez-Brunete, C., García-Valcárcel, A.I., and Tadeo, J.L Determination of...

Ngày tải lên: 12/08/2014, 04:22

... using extraction times well short of equilibrium times [37,45] With a extraction time and 100 mm PDMS fiber, analysis of a sample took less than 10 min, with MDLs in the order of 10 ng=L Chafer-Pericas ... pesticides or target analysis of a predefined set of compounds For the screening of a wide range of TABLE 9.1 Frequently Used GC and LC Methods for Analysis of Pesticides in Water and Their Relative Ranking ... screening of 39 EPA Method 507 nitrogen- and phosphorus-containing pesticides in water Jackson and Andrews [44] evaluated the use of SPME under nonequilibrium conditions for analysis of organochlorine...

Ngày tải lên: 12/08/2014, 04:22

... al.,31 and Kumari et al.52 for the analysis of current-used pesticides in air and rainwater The detection and analysis by ECD was commonly used for the analysis of organochlorine pesticides and ... analysis of a great number of samples and MS–MS enables the analysis of pesticides at trace level in the presence of interfering compounds without losing identification capability because of a drastic ... for the analysis of VOCs in indoor and outdoor atmospheres Thermal desorption for the analysis of pesticides has already been described for the volatile and stable pesticides trifluralin and triallate60,61...

Ngày tải lên: 12/08/2014, 04:22

... Examples of Reported Residue Levels (mg=kg Fat) of the Sum of DDT and Its Degradation Products DDE and DDD (SDDT) and Sum of HCH Isomers (SHCH) in Human Breast Milk Origin of Samples (Year of Sampling) ... residues of SDDT and SHCH were found in 94% and 70% of the samples, respectively A total of 186 samples of 20 different brands were analyzed The mean level of SDDT was found to be 300 mg=kg fat and ... of the tomatoes, 80% of the eggplants and cucumbers, and 50% of the apples and grapes The concentrations ranged from 0.002 to 0.29 mg=kg The potatoes of 50% contained fenitrothion at a mean of...

Ngày tải lên: 12/08/2014, 04:22

... calculation of the toxicity of mixtures of pesticides with similar modes of action (CA) and dissimilar modes of action (IA), respectively The research project of Prediction and Assessment of the Aquatic ... of the samples that is related to the number of samples and the selection of sampling stations intended within the objectives of the study The sampling process of taking random grab samples and ... PAS continuously integrate the air burden of pesticides and give real- time or near -time assessment of the concentration of pesticide in air [8,22,28] Most of the passive air sampling measurements...

Ngày tải lên: 12/08/2014, 04:22

... nmol of unlabelled lysoPC (egg yolk; containing predominantly 16:0 or 18:0) The standard incubation time was 30 and the reaction was stopped by adding 980 µl of a mixture of ice cold chloroform:methanol:water ... Hellgren LI, Selldén G, Sandelius AS: Effects of moderately enhanced levels of ozone on lipid composition and dynamical properties of the plasma membranes of leaves of garden pea Physiol Plant ... seeds of the transformed A thaliana were kindly provided by Prof I HaraNishimura [31] All chemicals and organic solvents were of analytical grade Lipid standards, polyethylene glycol (PEG) and...

Ngày tải lên: 12/08/2014, 05:20

... the beginning of CVVH (T0) and at the following times, according to the kinetics of PCT and to the lowest degree of clotting formation on the dialyzer membrane during these intervals of sampling: ... (T60′) and after hours (T6h) of CVVH, Ci, Co and Cuf; and after 12 hours, after 24 hours and every 24 hours during days (J1–J4) of CVVH In a preliminary study we determined the intra-assay and interassay ... Statistical analysis Results are presented as the mean ± standard deviation Comparisons of measured and calculated data were performed using analysis of variance or the Mann–Witney and Wilcoxon...

Ngày tải lên: 12/08/2014, 19:22

... various pH and irradiation sources (sunlight, darkness, indoor incandescent lighting and artificial UV lighting) Hydrolysis was found to be very much 143 pH-dependent irrespective of the type of water ... applied to analyze the residues of N-methylcarbamate and related compounds (propoxur and its metabolisms as model compounds) in biological materials (goldfish, tortoises and sea lettuce) Orthogonal ... decomposition was observed during the process The extracts of goldfish, tortoises and sea lettuce were stable and a 3-days delay prior to analysis was possible 144 ...

Ngày tải lên: 16/09/2015, 17:13