chemotherapy with or without radiotherapy

... This was most apparent for BrdU incorporation in cells cocultured with CDO and 5-FU (% BrdU incorporation normalized to control cells with SO: 72.6 ± 4.2% in cells treated with both drugs vs 90.2 ... progression [28,29]; for example, the correct formation of cyclin D/CDK complex is required for pRb hyper-phosphorylation, in order for it to release transcription factors necessary for G1/S transition ... level of incorporation in cultures with the two agents used simultaneously (observed) For example, the extent of BrdU incorporation into cells treated with CDO alone and into cells treated with VP16...

Ngày tải lên: 07/03/2014, 15:20

... editors AUTHORS Major portions of this revised report were prepared by ICF Incorporated, Fairfax, Virginia, under EPA Contract No 68-00-0102 While OHEA staff provided technical editing and incorporated ... in accordance with U.S Environmental Protection Agency policy and approved for publication Mention of trade names or commercial products does not constitute endorsement or recommendation for use ... the final report Robert Flaak, Assistant Staff Director of the SAB, whose efforts and professionalism in organizing and coordinating the two SAB reviews led to an improved and more useful product...

Ngày tải lên: 15/03/2014, 01:20

... protocol (oral morphine vs fentanyl patch, oral morphine vs buprenorphine patch, fentanyl vs buprenorphine), a comparison specific propensity scores will be estimated The propensity score method implies ... (RCT) will be carried out to formally compare the efficacy of alternative analgesic strategies, with particular emphasis on oral morphine as comparator and buprenorphine patch as experimental ... Hammerlid E, Kaasa S, Sprangers MA, Bjorner JB, for the EORTC Quality of Life Group: The development of the EORTC QLQ-C15-PAL: A shortened questionnaire for cancer patients in palliative care...

Ngày tải lên: 20/06/2014, 15:20

... for 14 and 28 days Tumor areas were measured every days using a caliper, and the tumor area was calculated according to the formula: tumor volume (mm3) = d2 x D/2, where d and D were the shortest ... for 90 at 37°C The reaction was quenched by washing with terminating buffer (300 mM sodium chloride and 30 mM sodium citrate) for 15 After blocking with normal serum, sections were incubated with ... prior joining the company He had been the founding Editor and Editor-in-Chief of Biological Signals and Biological Signals and Receptors, Adjunct Professor of University of Toronto and is Honorary...

Ngày tải lên: 08/08/2014, 18:20

... a problem as there is no apparent tumour and therefore unknown receptors For this reason chemotherapy with or without immunosupression is more commonly the treatment of choice There are no published ... local infiltration or metastatic spread They are thought to be due to either inappropriate secretion of hormones or the production of anti-tumoral antibodies that cross react with normal tissue antigens ... markers correlated with her tumour load Publish with Bio Med Central and every scientist can read your work free of charge "BioMed Central will be the most significant development for disseminating...

Ngày tải lên: 09/08/2014, 07:22

... treated with radiotherapy or chemoradiation [11] Similarly, Le and co-workers reported that high OPN plasma levels are associated with tumor hypoxia in head and neck squamous cell carcinomas and correlate ... after transfection Cells were either untreated (UT) or treated with OPN specific siRNA Mix and OpnS or with nonsense siRNA (non) with and without irradiation at Gy The Western blot shows one ... suspended in 300 μl of RPMI without FCS and were added to the upper chamber (membrane filter with μm pore size), and the bottom chamber was filled with ml of RPMI supplemented with 20% FCS as chemoattractant...

Ngày tải lên: 09/08/2014, 09:20

... propranolol and /or radiotherapy on COX-2, VEGF, EGFR and NF-B (p65) proteins SGC-7901 (A) and BGC-823 (B) cells were treated with/ without isoproterenol or propranolol for 24 hr prior to radiotherapy ... Discussion Gastric cancer is one of the major causes of cancer mortalities in the world, and radiotherapy is an important treatment for gastric cancer patients with a high risk of recurrence As we ... radiation with or without propranolol (50 μmol/L) 24 hr before irradiation Propranolol administration before irradiation of BGC-823 (A) and SGC-7901 (B) cells; BGC-823 (C) and SGC-7901 (D) cells with...

Ngày tải lên: 09/08/2014, 09:20

... were irradiated (RT) with 2, 5, or 10 Gy (A-C) or treated with 1-100 µM ErPC3, as indicated (D-F) 48 h after treatment a WST-1 Assay was performed The absorption correlates with the number of viable ... associated with malignant transformation characterized by accelerated tumor growth, metastasis, and angiogenesis Moreover, activated Akt decreases sensitivity of tumor cells to chemotherapy and radiotherapy ... and radiotherapy Importantly, Akt is embedded into a highly complex network of upstream regulators and downstream effector proteins and it is still unclear whether targeting the kinase itself or...

Ngày tải lên: 09/08/2014, 09:20

... immunofluorometric assay, with the MAb 6E8 as a capture antibody for TATI and a europium (Eu) labelled antibody 11B3 as a tracer [2,15] Fluorescence was measured with a 1420 VIKTOR2 time-resolved fluorometer ... series were constructed; one biopsy TMA with × mm cores from biopsies with normal tissue (sampled cm from the tumour) and cancer, respectively, and one TMA with normal and cancerous tissue from the ... tumour-associated trypsin inhibitor (TATI), also called pancreatic secretory trypsin inhibitor (PSTI) and serine protease inhibitor Kazal type (SPINK1), is a biomarker of poor prognosis in colorectal cancer patients,...

Ngày tải lên: 09/08/2014, 09:21

... due to more conformal dose distributions with the high dose region confined to the target volume and sparing of organs-at-risk [6,7] The correlation between the volume of the rectum within the ... solid organs like most tumors, liver or parotid gland the DVH is based on the volume encompassed by the outer contour of the organ For "hollow" organs like the rectum or bladder, the use of the ... cylindrical model for the rectum Fiorino et al described substantial differences between DVH and DWH for a "full" rectum but only small differences for an "empty" rectum For patients with a distended...

Ngày tải lên: 09/08/2014, 10:21

... the t-scores for RE-score comparison between ER- and ER+ samples The t-scores for 470 miRNAs were calculated by comparing their RE-scores for ER- samples with those for ER+ samples The t-score distributions ... FDR

Ngày tải lên: 09/08/2014, 20:20

... Rodriguez-Guardado A: AIDS-related Kaposi’s sarcoma with chylothorax and pericardial involvement satisfactorily treated with liposomal doxorubicin AIDS (London, England) 2002, 16:806 Page of ... Waser B, Schaer JC, Laissue JA: Somatostatin receptor sst1-sst5 expression in normal and neoplastic human tissues using receptor autoradiography with subtype-selective ligands Eur J Nucl Med 2001, ... and so on, are available for the treatment of several kinds of disorders Octreotide was the first developed analogue and is widely used for symptomatic treatment of hormone secreting neuroendocrine...

Ngày tải lên: 10/08/2014, 10:20

... blocked by normal goat serum for 30 min, added with primary antibody solutions at 37°C for h, then cultured at room temperature overnight After washing with PBS, cells were added with secondary ... CO2 for h After washing off the un-adhered cells, the 96-well plates were fixed by 4% paraformaldehyde for 30 min, stained with 0.5% crystal violet (100 μL/well) for h, and then washed twice with ... conditions were 37°C for 15 min, 85°C for s, and 4°C for The sequences of CD44 gene primers were 5’-CCAATGCCTTTGATGGACCA-3’ for forward Page of primer and 5’-TGTGAGTGTCCATCTGATTC-3’ for reverse primer...

Ngày tải lên: 10/08/2014, 10:21

... wasted compared with the EPD treated mice Ovarian cancer has a poor prognosis With more than 60% of the patients presenting the disease in stage III or IV, combination chemotherapy with Platinum ... μg/mL, with final concentrations of 0.02 up to 0.2% DMSO In vivo pilot experiment An in vivo pilot experiment was performed with 20 BALB/c nude mice (Charles River Laboratories, France) In order ... countries, in spite of chemotherapy with Platinum derivates and /or Taxol after surgery Of the malignant epithelial tumors (>90% of all ovarian cancers), the serous papillary variants form the largest...

Ngày tải lên: 10/08/2014, 10:21

... respectively, at 4°C and subsequently with secondary antibodies for hour After wash with PBST, signals were visualized by incubation with ECL luminescence substrate and detected with Universal Hood2 Chem ... tumor growth of breast cancer cell was inhibited Combined treatment of ulinastatin plus docetaxel is more effective in anti-tumor invasion Therefore, the role of ulinastatin in the antitumor ... at 37°C in an incubator supplemented with 5% CO2 24 h later, cells in the upper chamber were removed with a cotton swab The remaining cells on the membrane were stained with 0.1% crystal violet...

Ngày tải lên: 10/08/2014, 10:21

... 1/3 scored as 1, more than 1/3 and less than 2/3 scored as 2, more than 2/3 scored as 3, without positive cell scored as Cells without brown staining scored as 0, with mild brown staining scored ... staining scored as 1, with moderate brown staining scored as 2, with intense brown staining scored as The final positive scores = positive rate score × staining intensity score, score was negative ... chamber with 200 μl fresh medium without FBS, added 500 μl medium with 20% FBS into each lower chamber, three duplicate wells were set up for each group After 12 h, fixated cells with methanol for...

Ngày tải lên: 10/08/2014, 10:21

... were 95°C for 30 s, and 40 cycles of 95°C for s and 60°C for 34 s The amplification specificity was evaluated with melting curve analysis Threshold cycle Ct, which correlates inversely with the ... incubation with the primary antibodies, membranes were washed and incubated with HRPlabelled goat anti-rabbit second antibody (Santa Cruz Biotechnology Inc., USA) in TBST for h Immunoreactivity ... [10] the cells were incubated for 72 h (invasion) or 36 h (migration) at 37°C in a 5% CO2 incubator, the cells on the top surface of the insert were removed by wiping with a cotton swab The cells...

Ngày tải lên: 10/08/2014, 10:21

... periods not correlate with the expression of DNMT1 directly [10] Therefore, whether over-expression of DNMT1 accounts for the only or key causes of hypermethylation of tumor suppressor genes remains ... temperature for h, and incubated with Rabbit antiDNMT1 and secondary antibody at 37°C for h respectively The Membranes were stained with an enhanced chemiluminescence solution Band intensities are normalized ... 96-well plates and cultured with siRNA-DNMT1 at 37°C in a humid chamber with 5% CO2 for 24 h 50 μl × MTT was then added to each well and incubated with cells at 37°C for h After removal of supernatant,...

Ngày tải lên: 10/08/2014, 10:21

... factor-receptor, and HER-2) or human tumor types (neuroblastoma, melanoma, lymphoma, epidermoid carcinoma, and breast carcinoma) or tumor sites (subcutaneous versus systemic) The effect was correlated ... β-glucans with fluorescein to track their oral uptake and processing in vivo The orally administered β-glucans were taken up by macrophages via the Dectin-1 receptor and was subsequently transported ... macrophages via the Dectin-1 receptor with or without TLR-2/6 The large β-glucan molecules are then internalized and fragmented into smaller sized β-glucan fragments within the macrophages They are...

Ngày tải lên: 10/08/2014, 22:20