chemokines recruitment of macrophages and wallerian degeneration
báo cáo hóa học: " Sinomenine inhibits microglial activation by Ab and confers neuroprotection" doc
... kit), re-suspended in 300 μl of the wash buffer and analyzed in FACS Calibur using Cell Quest Pro Software and BD CBA software (Becton Dickinson, San Diego, CA) The standard curve was prepared according ... production of NO in these cells [26-28] Reactive oxygen species and nitric oxide have been implicated in the pathogenesis of AD The analysis of AD brain samples reveals evidence of ROS and NO production ... serum-starved for h and then treated with a mixture of 50% BV2-conditioned medium and 50% fresh DMEM For MTT assay, cells were treated for 44 h (before addition of MTT), and for the TUNEL assay,...
- 11
- 343
- 0
Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... induction Effects of a-T and its derivatives on the enhancement by TS of LPS/IFN-induced NO production We compared the effects of a-T and its derivatives TA and TN with that of TS on LPS/IFN-induced ... production with Ro31-2880 and GF109203X were approximately 70 and 30%, respectively Effects of TS on the amount of PKCa We further examined the effects of TS on the amounts of PKCa and PKCb by Western ... upregulation of PKCa DISCUSSION Fig Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors (Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFNinduced NO production in VSMC (A), and Western...
- 6
- 494
- 0
Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc
... nm in UV and CD spectra of metallothioneins devoid of any aromatic residues that Fig Wavelength and time scans of the CD of MT2 in the presence and absence of NO and GSH/GSSG CD spectra of 100 ... traces and online element-selective detection of the SEC–ICPMS characterization of pure 20 lM Cd5Zn2-MT2 and mixtures of MT2 + NO (1 +11) and MT2 + GSH + NO (1 +25 +10) The amount of zinc and cadmium ... concentration of mM For reduced and oxidized glutathione, concentrations of mM were used CD spectra of the range between 230 and 300 nm are shown in Fig 1A The Cd-S charge transfer band at 260 nm...
- 9
- 398
- 0
Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot
... as RAW264.7 and J774.1 Production of IFN-c and IL-12 by murine PEC upon LPS stimulation We then investigated the production of IFN-c and related cytokines upon LPS stimulation of PEC and RAW264.7 ... suppression of the LPS-induced production of NO and IFN-c indicates that the signals of LPS for the production of these mediators are transduced through CD14 Stimulatory effect of IFN-c and IL-12 ... Preparations of recombinant IFN-c, IL-12 and IL-18 were added to PEC cultures of HeN and HeJ mice, and production of NO in the culture supernatant was measured As shown in Fig 4A, a significant effect of...
- 10
- 395
- 0
Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... homeostasis A B Fig Levels of NOS isozymes and HSP90 in brain and aorta of NMS rats treated with HSD and C.I.1 Aliquots of brain soluble material (20 lg protein) and of aorta total lysate (50 ... formation and stabilization of proteolytically resistant complexes of NOS with HSP90 are crucial in determining the in vivo resistance of NOS and HSP90 to calpain degradation Results Levels of HSP90 and ... brain and aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform,...
- 11
- 344
- 0
báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
... expression of both TrkA and p75NTR [71,72] as well as the convergence of TrkA and p75NTR-mediated signaling impinging upon NF-κB [73] Page 10 of 13 (page number not for citation purposes) Journal of ... design and coordination and provided the expertise for RTPCR and withdrawal experiments HUS sub-cloned the PC12p75NTR(-) cells and participated in study design and result interpretation of experiments ... Chandross KJ: Nerve injury and inflammatory cytokines modulate gap junctions in the peripheral nervous system Glia 1998, 24:21-31 Stoll G, Jander S, Myers RR: Degeneration and regeneration of...
- 13
- 447
- 0
Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf
... Western blot analysis of three isoforms of NOS in EAE The expression of nNOS (Fig 2A), eNOS (Fig 2B) and Fig Western blot analysis of constitutive neuronal NOS (nNOS) (A) and endothelial NOS (eNOS) ... lesions Fig Immunostaining of three isoforms of NOS in the spinal cords of normal (4A-4C) and EAE-affected rats (4D-4F) The immunoreactivity of nNOS (4A), eNOS (4B), and iNOS(4C) was scarcely ... Gonzalez-Hernandez and Rustioni [18] reported that the three isoforms of NOS including nNOS, eNOS and iNOS, exert a beneficial effect on peripheral nerve regeneration In the course of acute EAE...
- 7
- 290
- 0
Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx
... analysis of three isoforms of NOS in the ovary The expression levels of nNOS, eNOS, and iNOS were assessed semiquantitatively by densitometry after Western blotting Immunoreactivity for all three isoforms ... cells nNOS (H) and eNOS (I) were expressed in the granulosa cells, oocytes, and theca interna of multilaminar follicles iNOS (J) was expressed in the granulosa cells and oocytes of multilaminar ... cells and theca of antral follicles A-C: Counterstained with hematoxylin Scale bars = 30 µm Table Immunohistochemical localization of neuronal (nNOS), endothelial (eNOS), and inducible (iNOS) isoforms...
- 5
- 245
- 0
Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx
... design CB, JC and JPP participated in the acquisition of data CB, JMP, JC and JPP participated in the analysis and interpretation of data CB, JM-P and JPP prepared the manuscript CB, JM-P and JC participated ... chondrocytes [4] They can also inhibit the stimulating action of IL-1β on stromelysin and collagenase and inhibit production of IL-6, IL-8 and prostaglandin E2 [5] In addition, it was demonstrated that ... superficial zone of cartilage corresponds to the superficial and the upper intermediate layers, and the deep zone to the lower intermediate and deep layers The total number of chondrocytes and those...
- 9
- 547
- 0
Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" potx
... stimulation, and result in suppression of phosphorylation of MAPKs such as p38 MAPK and ERK1/2 and NF-B activation Conclusions Our results strongly suggest that inhibitory action of CAM and M-4 on ... concept and design of the study, and to the manuscript writing AF, NS, KH and TH performed surgical operation and cell culture KA examined NO levels, iNOS expression, and assayed phosphorylation of ... Influence of CAM and M-4 on iNOS levels in NPFs after LPS stimulation The fifth set of experiments was done to examine the influence of CAM and M-4 on iNOS production from Furuya et al Journal of Inflammation...
- 12
- 376
- 0
Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" pptx
... stimulation, and result in suppression of phosphorylation of MAPKs such as p38 MAPK and ERK1/2 and NF-B activation Conclusions Our results strongly suggest that inhibitory action of CAM and M-4 on ... concept and design of the study, and to the manuscript writing AF, NS, KH and TH performed surgical operation and cell culture KA examined NO levels, iNOS expression, and assayed phosphorylation of ... Influence of CAM and M-4 on iNOS levels in NPFs after LPS stimulation The fifth set of experiments was done to examine the influence of CAM and M-4 on iNOS production from Furuya et al Journal of Inflammation...
- 12
- 301
- 0
Báo cáo y học: " Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and systemic lupus erythematosus" potx
... regulates many steps of T cell activation, the production of cytokines, such as IL-2, and mitochondrial hyperpolarization and mitochondrial biogenesis NO regulates mammalian target of rapamycin (mTOR) ... of rapamycin (mTOR) is a serine/ threonine protein kinase and a sensor of the Page of mitochondrial transmembrane potential that regulates protein synthesis, cell growth, cell proliferation and ... of genomic DNA and TCRζ transcripts showed mutations in the coding region of TCRζ from lupus T cells [39] There is a direct interaction between HRES-1/Rab4, CD4 and TCRζ Rapamycin treatment of...
- 6
- 295
- 0
Báo cáo y học: "Involvement of nitric oxide (NO) in cough reflex sensitivity between non-sensitized and OVA-sensitized guinea pigs" pptx
... Page of Non-sensitized and OVA-sensitized guinea pigs were divided into four groups: control (normal saline) group and groups receiving the non-selective NOS inhibitor LNAME at doses of 3, 10, and ... Figure and Non-sensitized and OVA-sensitized guinea pigs were divided into three groups: control (normal saline) group and groups receiving the selective iNOS inhibitor ONO1714 at doses of 0.1 and ... doses of 0.1 and 0.3 mg/kg Vehicle (normal saline) and ONO1714 solution were first intraperitoneally administered 0.5 h prior to the measurement of cough reflex sensitivity to inhaled capsaicin, and...
- 9
- 306
- 0
Báo cáo y học: "Time course of nitric oxide synthases, nitrosative stress, and poly(ADP ribosylation) in an ovine sepsis model" pot
... http://ccforum.com/content/14/4/R129 Page of 10 Materials and methods This study was approved by the Animal Care and Use Committee of the University of Texas Medical Branch and conducted in compliance with the guidelines of the ... early overexpression of eNOS and iNOS, enhanced NOS activity, and increased expression of markers of nitrosative stress and DNA damage in lung tissue The pulmonary response to ALI and sepsis in sheep ... to over-expression of NOS, but also to enhanced activity of constitutively expressed enzymes Therefore, to profoundly understand the roles of different NOS isoforms in ALI and sepsis, we measured...
- 10
- 247
- 0
The role of nitric oxide and other gaseous mediators in cardiovascular disease models; emphasis on septic shock
... Effect of flurbiprofen or nitroflurbiprofen on NF-κB activation in the liver of rats at h after LPS injection Figure 6-7 Effect of flurbiprofen or nitroflurbiprofen on the proteolytic activity of ... LPS pre-treatment VII List of Figures with either equivalent volume of vehicle, flurbiprofen or nitroflurbiprofen Figure 6-2 Effect of flurbiprofen or nitroflurbiprofen on MPO activity Figure ... of NO at rates that may only be limited by the availability of substrates and cofactors Mainly for these reasons the constitutive and inducible isoforms of NOS are often referred to as low- and...
- 222
- 297
- 0
The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 2
... consisted of mL of hypnorm (Jansen Pharmaceutica, Beerse, Belgica), which contained 0.315 mg of fentanyl and 10 mg of fluanisone, and mL of midazolam (Roche, Basel, Switzerland) which contained mg of ... shock model: The role of NO and PGE2 and the therapeutics effects of conservative fluids, NOS and COX-2 inhibitors 45 4.1 Introduction The up-regulation of nitric oxide (NO) and cyclooxgenase-2 ... up-regulation as a result of an inflammatory response (Tsukada et al., 2000, Knoferl et al., 2001) Two isoforms of COX have been identified namely COX-1 and COX-2 COX-1 is a constitutive isoform that is...
- 138
- 423
- 0
The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 1
... groups of rats Figure 4.3F Plasma GOT levels at 24, 48 and 72 hours in different 60 groups of rats Figure 4.4.1 Hemotoxylin and eosin stain of Kidneys 62 Figure 4.4.2 Hemotoxylin and eosin stain of ... analysis of major organ injury of different 22 groups of rats Table 2.2 Creatinine and GOT levels in different groups of rats 22 Table 3.1 Semi-quantitative analysis of major organ injury of different ... MABP of different groups of rats 34 Figure 3.3 Nitrate/nitrite in different groups of rats 36 Figure 3.4 Creatinine levels of rat brain in different groups of rats 37 Figure 3.5 GOT levels of rat...
- 16
- 212
- 0
Role of nitric oxide in wound healing facilitatory effects of nitrosoglutathione a nitric oxide donor on the extracellular matrix deposition characteristics of wound healing
... accumulation of macrophages and migration of fibroblasts, deposition of connective tissue and angiogenesis The granular appearance of the tissue is due to the numerous newly formed blood vessels Macrophages, ... glycoprotein with a molecular weight of 30,000, composed of A and B chains It exists as both a heterodimer and a homodimer The major platelet and macrophage isoforms (AB and BB) of PDGF were found to stimulate ... Keratinocytes; macrophages TGF- and - Platelets; macrophages TGF- Macrophages IL-1 and Neutrophils TNF- Neutrophils Primary target cells and effect Keratinocyte motogen and mitogen Keratinocyte motogen and...
- 258
- 672
- 0
Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... importance of Fas and FasL [15], and that of TNF-R1 and TNF-α[16], in preeclampsia Thus, hypoxia-induced changes in the expression of both proapoptotic and antiapoptotic proteins, and 43 the balance of ... induction of apoptosis, were detected Furthermore, increased expression of antiapoptotic proteins such as Bag-1, Hsp70, and Bcl-X, and decreased expression of proapoptotic proteins such as Bid and ... expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig 2) Table Altered protein expression of JAR cells...
- 9
- 499
- 0
Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... prepared, and the phosphorylation of eIF2a and the expression of ATF4 and CHOP proteins were examined by western blotting with specific antibodies (B) Western blot analysis of the expression of CHOP ... expression of proinflammatory cytokines, inhibition of insulin signaling, induction of endoplasmic reticulum (ER) stress, and promotion of cell death, mainly by apoptosis [6–12] On the other hand, unsaturated ... combinations of saturated (stearate, 18:0) and unsaturated (oleate, 18:1 cis) fatty acids for various time periods, and cell proliferation, toxicity, induction of ER stress and apoptosis and lipid...
- 12
- 721
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25