checklist for phenotypic characterization of chickens
Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... the actual site of proteolytic cleavage, data interpretation and the design of followup analyses are straightforward COFRADIC-based sorting of N-glycosylated peptides Glycosylation of asparagines ... COFRADIC procedure was applied to a trypsin digest of 10 lL of mouse serum depleted for its three most abundant proteins (albumin, IgGs and transferrin), and resulted in the characterization of ... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing...
- 13
- 578
- 0
Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... materials, and that mechanical characterization of protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterization of biological molecules ... strain Δε0 for a given position vector r of a protein structure is in the form of u ( r ) = Δε ⋅ r (1) Accordingly, the position vector r* of a protein structure after application of discrete,...
- 29
- 367
- 0
Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt
... approaches offer generic advantages for the analysis of protein complexes both in terms of dynamic range (i.e how little of one protein can be identified in the presence of how much of a mixture of other ... expression of downstream target genes of the Wnt-signaling pathway [9–11] Both interactions are largely independent of each other and knowledge of both yields information on different aspects of beta-catenin ... high number of peptides of the complete protein that would be required for an unambiguous identification Clustering of ESTs and gene prediction can make this information available for searching...
- 9
- 415
- 0
PHENOTYPIC CHARACTERIZATION OF ANIMAL GENETIC RESOURCES pdf
... comprehensive list of variables for describing the phenotypic and genetic characteristics of cattle, sheep, goats and chickens as the basis for systematic phenotypic characterization of these species ... part of phenotypic characterization Box How to complement genetic characterization with phenotypic characterization – an example An integrated phenotypic and genetic characterization study of three ... case is one of the roles of phenotypic characterization and is a prerequisite for effective assessment of AnGR diversity and determining whether or not it is being eroded Phenotypic characterization...
- 158
- 487
- 0
Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx
... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... number of paired samples, a firm conclusion cannot be drawn Discussion Several recent studies showed that the phenotypic and genotypic characterization of CTCs may provide valuable information of ... medians of CTCs of epithelial cancer patients and melanoma patients P < 0.05 was considered significantly different Results Performance of three different enrichment methods Purity and recovery of...
- 8
- 510
- 0
báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx
... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... number of paired samples, a firm conclusion cannot be drawn Discussion Several recent studies showed that the phenotypic and genotypic characterization of CTCs may provide valuable information of ... medians of CTCs of epithelial cancer patients and melanoma patients P < 0.05 was considered significantly different Results Performance of three different enrichment methods Purity and recovery of...
- 8
- 533
- 0
báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx
... proteins This ultimately Another drawback for the use of EDCHCl/sulfo-NHS for the formation of stable bioconjugates is the lack of specificity on the protein of interest As numerous amine functional ... conjugate formation Bioconjugation of proteins to carboxylated QDs have been performed with the use of EDC alone [7] Despite the simplicity of a one-step reaction, the drawback involves a degree of ... net-charge of the conjugate The increase in peak width of the QD-BSA can be attributed to a number of factors, including the polydispersity Page of 15 (page number not for citation purposes) Journal of...
- 15
- 301
- 0
báo cáo khoa học: " A new model for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation" pptx
... direction of fire The tests were performed with gelatin blocks (n = 12) They consisted of 20% porcine gelatin (Merck, Darmstadt, Germany), and water and had an edge length of 12 × 12 × 18 cm Page of ... examination of the infiltration depth of barium titanate particles within the ruptures of a temporary cavity in the gelatin block revealed a deposition of particles along the entire bullet path for both ... both types of projectiles examined In the case of the soft point projectile, there were no significant differences between the size of the temporary cavity and the infiltration depth of the barium...
- 5
- 573
- 0
Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis" pdf
... of antiCCP-antibodies or RF) and genetic (possession of SE alleles) parameters predictive of more severe RA Unfortunately, information on the degree of radiographic joint damage at the time of ... achieved with the IMPACT platform, we performed 21 replicate measurements of each of nine markers within one run on the IMPACT platform The intra-assay coefficients of variance (CV) ranged from ... coefficients of 0.92 for RF and 0.97 for CRP (Figures 2b and 2c) Analysis of the bone-turnover markers with IMPACT was previously described, the results of which correlated well with those of corresponding...
- 13
- 407
- 0
Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films
... SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MECHANICAL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Preface This dissertation is submitted for the degree of Doctor of Philosophy ... in the Department of Mechanical Engineering, National University of Singapore (NUS) under the supervision of Associate Professor Zeng Kaiyang To the best of my knowledge, all of the results presented ... Piezoresponse Force Microscopy 18 2.1 Working Principle of Piezoresponse Force Microscopy 18 2.1.1 Experimental Setup 2.2 Theory of PFM 18 21 2.2.1 Capacitive forces 23 2.2.2 Electromechanical Forces...
- 171
- 267
- 0
Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase the risk of side effects of the statins [3,7].Therefore the developments of novel pharmaceutical formulations ... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... hematocrite of washed erythrocytes was adjusted by PBS to 45% In ml eppendorff tubes, 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP,...
- 9
- 829
- 0
Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... performance of PVDF-SPS composite membrane should be done The overall findings suggest that the PVDF-SPS membrane offers the potential for improving the performance of the DMFCs, this kind of ... active energy (Ea) of the membranes were calculated, 18.58 kJ.mol−1 for Nafion-117 and 20.05 kJ.mol−1 for PVDF-SPS membrane, respectively Ea of the PVDFSPS membrane is close to that of the Nafion-117 ... hydrophobic property of PS polymer promoted the formation of these polymer aggregates As can be seen, the thermally induced polymerization of styrene, as well as the blending of PS and SPS polymers,...
- 14
- 596
- 1
Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
... breakdown of lignocellulosic biomass for the establishment of a robust and cost-efficient process for production of cellulosic ethanol Acknowledgements Financial support by the Center for Bioprocessing ... Results and discussion 3.1 Compost characterization A summary of the YWC characteristics is shown in Table All compost samples were of black color owing to the formation of humic substances, carbon ... cellulose and xylan are present in the form of waste paper and plant residues as major constituents of municipal and yard waste [45] For instance, a strain of Scytalidium thermophilum producing...
- 14
- 525
- 0
Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... widely used by the ECR community for the extraction of singly charged ion beams The ‘2D Poisson’ software package has been used in the present work for simulation of the magnetic field pattern using ... Acquisition and analysis of Langmuir probe characterization for ECR plasma Indian J Phys 80: 1011–1015 Jain S K, Jain A, Hannurkar P R, Kotaiah S 2007 Characterization of plasma parameter, first ... ion source uses the principle of adiabatic invariance for mirror reflection, and high magnetic field mode of operation to successfully generate and confine plasma Design of the electromagnet The magnetic...
- 8
- 650
- 0
Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... unknown but in view of the extensive differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation ⁄ desensitization [20–23] and patterns of expression ... isoform of the human thromboxane A2 receptor is a target for prostacyclin-mediated desensitization J Biol Chem 275, 20412–20423 23 Reid HM & Kinsella BT (2003) The alpha, but not the beta, isoform ... Characterization of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms Eur J Biochem 269, 4058–4073 26...
- 18
- 509
- 0
Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... transcription start sites for the integrin a3 subunit gene, a modified method of 5¢-RACE using a cap site-labeled cDNA library was employed, recently developed for rapid examination of 5¢-end of genes [33] ... The members of the Ets-family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T and control the expression of numerous genes that are critical for various ... Preparation of nuclear extracts from MKN1 cells and EMSA were performed essentially as described by Ko et al [34] The DNA fragments containing the putative Etsbinding sequence of the 5¢-flanking region of...
- 9
- 562
- 0
Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... C7¢–C8¢ double bond of linear and monocyclic carotenoids to be an additional novel cleavage site of OsCCD1, leading to geranial and indicating a novel plant route for the formation of this widespread ... low amount of the C14 dialdehyde is probably a result of instability The formation of b-ionone from b-apo-8¢-carotenal (C30) and b-apo10¢-carotenal (C27) suggested that the cleavage of the C9–C10 ... 741 A novel route for geranial formation A Ilg et al tra with the NIST library HPLC analyses of the corresponding cell pellets revealed the accumulation of a complex mixture of compounds, tentatively...
- 12
- 497
- 0
Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... Fernandez-Montalvan et al Biochemical characterization of USP7 Fig Enzymatic characterization of USP7 (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC (j), SUMO-1-AMC (d) and Nedd8-AMC ... inhibitor of jB kinase, a regulatory role can be presumed for USP7 phorsphorylation The identification of K869 as the ubiquitination site of USP7 represents additional evidence for the interaction of ... Institutes for Biomedical Research (NIBR) Journal compilation ª 2007 FEBS 4261 ´ ´ A Fernandez-Montalvan et al Biochemical characterization of USP7 Table Kinetic parameters for the hydrolysis of Ub-AMC...
- 15
- 592
- 0
Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... trans conformers of their substrates [22,23] Taking the cis ⁄ trans content of Bip-Pro (78% ⁄ 22%) into account, we obtained Ki trans values for Bip-Pro of 5.2 lm in Caco-2 cells and of 0.75 lm ... cells For further functional characterization of Bip-[3H]Pro uptake we therefore used SKPT cells Fig Substrate saturation kinetics of Bip-Pro and Gly-Sar transport in SKPT cells (A) Uptake of Bip-[3H]Pro ... substrate for peptide transporters Table Inhibition constants (Ki) of different substrates for the inhibition of Bip-[3H]Pro and [14C]Gly-Sar uptake in SKPT cells Uptake of Bip-[3H]Pro (4 nM, h) or of...
- 10
- 490
- 0
Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
... regeneration of the active form of reduced RNaseA Data represent the mean ± SD for four experiments The data for GmPDIS-1, GmPDIS-2 and GmPDIM are from Wadahama et al [26,27] (B,C) Chaperone activity of ... Nucleotide sequences of the ORF of both PDI genes were exactly the same as those of cDNAs cloned in this study Comparisons of soybean genomic sequences of GmPDIL-1 or GmPDIL-2 with those of Arabidopsis ... Program for Promotion of Basic Research Activities for Innovative Biosciences and a Grant-in-Aid for Exploratory Research from the Ministry of Education, Culture, Sports, Science and Technology of...
- 15
- 424
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25