checklist for phenotypic characterization of cattle

... the actual site of proteolytic cleavage, data interpretation and the design of followup analyses are straightforward COFRADIC-based sorting of N-glycosylated peptides Glycosylation of asparagines ... COFRADIC procedure was applied to a trypsin digest of 10 lL of mouse serum depleted for its three most abundant proteins (albumin, IgGs and transferrin), and resulted in the characterization of ... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing...

Ngày tải lên: 18/02/2014, 16:20

... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... materials, and that mechanical characterization of protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterization of biological molecules ... strain Δε0 for a given position vector r of a protein structure is in the form of u ( r ) = Δε ⋅ r (1) Accordingly, the position vector r* of a protein structure after application of discrete,...

Ngày tải lên: 22/02/2014, 08:20

... approaches offer generic advantages for the analysis of protein complexes both in terms of dynamic range (i.e how little of one protein can be identified in the presence of how much of a mixture of other ... expression of downstream target genes of the Wnt-signaling pathway [9–11] Both interactions are largely independent of each other and knowledge of both yields information on different aspects of beta-catenin ... high number of peptides of the complete protein that would be required for an unambiguous identification Clustering of ESTs and gene prediction can make this information available for searching...

Ngày tải lên: 31/03/2014, 07:20

... comprehensive list of variables for describing the phenotypic and genetic characteristics of cattle, sheep, goats and chickens as the basis for systematic phenotypic characterization of these species ... part of phenotypic characterization Box How to complement genetic characterization with phenotypic characterization – an example An integrated phenotypic and genetic characterization study of three ... case is one of the roles of phenotypic characterization and is a prerequisite for effective assessment of AnGR diversity and determining whether or not it is being eroded Phenotypic characterization...

Ngày tải lên: 31/03/2014, 22:20

... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... number of paired samples, a firm conclusion cannot be drawn Discussion Several recent studies showed that the phenotypic and genotypic characterization of CTCs may provide valuable information of ... medians of CTCs of epithelial cancer patients and melanoma patients P < 0.05 was considered significantly different Results Performance of three different enrichment methods Purity and recovery of...

Ngày tải lên: 18/06/2014, 19:20

... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... number of paired samples, a firm conclusion cannot be drawn Discussion Several recent studies showed that the phenotypic and genotypic characterization of CTCs may provide valuable information of ... medians of CTCs of epithelial cancer patients and melanoma patients P < 0.05 was considered significantly different Results Performance of three different enrichment methods Purity and recovery of...

Ngày tải lên: 20/06/2014, 03:20

... proteins This ultimately Another drawback for the use of EDCHCl/sulfo-NHS for the formation of stable bioconjugates is the lack of specificity on the protein of interest As numerous amine functional ... conjugate formation Bioconjugation of proteins to carboxylated QDs have been performed with the use of EDC alone [7] Despite the simplicity of a one-step reaction, the drawback involves a degree of ... net-charge of the conjugate The increase in peak width of the QD-BSA can be attributed to a number of factors, including the polydispersity Page of 15 (page number not for citation purposes) Journal of...

Ngày tải lên: 11/08/2014, 00:22

... direction of fire The tests were performed with gelatin blocks (n = 12) They consisted of 20% porcine gelatin (Merck, Darmstadt, Germany), and water and had an edge length of 12 × 12 × 18 cm Page of ... examination of the infiltration depth of barium titanate particles within the ruptures of a temporary cavity in the gelatin block revealed a deposition of particles along the entire bullet path for both ... both types of projectiles examined In the case of the soft point projectile, there were no significant differences between the size of the temporary cavity and the infiltration depth of the barium...

Ngày tải lên: 11/08/2014, 20:21

... of antiCCP-antibodies or RF) and genetic (possession of SE alleles) parameters predictive of more severe RA Unfortunately, information on the degree of radiographic joint damage at the time of ... achieved with the IMPACT platform, we performed 21 replicate measurements of each of nine markers within one run on the IMPACT platform The intra-assay coefficients of variance (CV) ranged from ... coefficients of 0.92 for RF and 0.97 for CRP (Figures 2b and 2c) Analysis of the bone-turnover markers with IMPACT was previously described, the results of which correlated well with those of corresponding...

Ngày tải lên: 12/08/2014, 17:22

... SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MECHANICAL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Preface This dissertation is submitted for the degree of Doctor of Philosophy ... in the Department of Mechanical Engineering, National University of Singapore (NUS) under the supervision of Associate Professor Zeng Kaiyang To the best of my knowledge, all of the results presented ... Piezoresponse Force Microscopy 18 2.1 Working Principle of Piezoresponse Force Microscopy 18 2.1.1 Experimental Setup 2.2 Theory of PFM 18 21 2.2.1 Capacitive forces 23 2.2.2 Electromechanical Forces...

Ngày tải lên: 10/09/2015, 08:41

... adiponectin isoforms: AdipoR1 is a receptor for globular and full-length adiponectin isoforms whereas AdipoR2 has a higher affinity for full-length adiponectin isoforms than globular adiponectin (Kadowaki ... promoting mobilization of nonesterified fatty acids (NEFA), amino acids and sparing of glucose for increased nutrient demand of mammary gland for milk production (Bell, 1995) The role of adiponectin ... in-house for the measurement of adiponectin in bovine samples (Mielenz et al., 2013) Based on the principle of competition in antigen-antibody binding, the competitive ELISA is one of the formats of...

Ngày tải lên: 19/11/2015, 16:40

... inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase the risk of side effects of the statins [3,7].Therefore the developments of novel pharmaceutical formulations ... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... hematocrite of washed erythrocytes was adjusted by PBS to 45% In ml eppendorff tubes, 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP,...

Ngày tải lên: 25/10/2012, 11:10

... performance of PVDF-SPS composite membrane should be done The overall findings suggest that the PVDF-SPS membrane offers the potential for improving the performance of the DMFCs, this kind of ... active energy (Ea) of the membranes were calculated, 18.58 kJ.mol−1 for Nafion-117 and 20.05 kJ.mol−1 for PVDF-SPS membrane, respectively Ea of the PVDFSPS membrane is close to that of the Nafion-117 ... hydrophobic property of PS polymer promoted the formation of these polymer aggregates As can be seen, the thermally induced polymerization of styrene, as well as the blending of PS and SPS polymers,...

Ngày tải lên: 05/09/2013, 16:11

... breakdown of lignocellulosic biomass for the establishment of a robust and cost-efficient process for production of cellulosic ethanol Acknowledgements Financial support by the Center for Bioprocessing ... Results and discussion 3.1 Compost characterization A summary of the YWC characteristics is shown in Table All compost samples were of black color owing to the formation of humic substances, carbon ... cellulose and xylan are present in the form of waste paper and plant residues as major constituents of municipal and yard waste [45] For instance, a strain of Scytalidium thermophilum producing...

Ngày tải lên: 05/09/2013, 16:11

... widely used by the ECR community for the extraction of singly charged ion beams The ‘2D Poisson’ software package has been used in the present work for simulation of the magnetic field pattern using ... Acquisition and analysis of Langmuir probe characterization for ECR plasma Indian J Phys 80: 1011–1015 Jain S K, Jain A, Hannurkar P R, Kotaiah S 2007 Characterization of plasma parameter, first ... ion source uses the principle of adiabatic invariance for mirror reflection, and high magnetic field mode of operation to successfully generate and confine plasma Design of the electromagnet The magnetic...

Ngày tải lên: 22/12/2013, 08:58

... unknown but in view of the extensive differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation ⁄ desensitization [20–23] and patterns of expression ... isoform of the human thromboxane A2 receptor is a target for prostacyclin-mediated desensitization J Biol Chem 275, 20412–20423 23 Reid HM & Kinsella BT (2003) The alpha, but not the beta, isoform ... Characterization of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms Eur J Biochem 269, 4058–4073 26...

Ngày tải lên: 19/02/2014, 16:20

... transcription start sites for the integrin a3 subunit gene, a modified method of 5¢-RACE using a cap site-labeled cDNA library was employed, recently developed for rapid examination of 5¢-end of genes [33] ... The members of the Ets-family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T and control the expression of numerous genes that are critical for various ... Preparation of nuclear extracts from MKN1 cells and EMSA were performed essentially as described by Ko et al [34] The DNA fragments containing the putative Etsbinding sequence of the 5¢-flanking region of...

Ngày tải lên: 21/02/2014, 03:20

... C7¢–C8¢ double bond of linear and monocyclic carotenoids to be an additional novel cleavage site of OsCCD1, leading to geranial and indicating a novel plant route for the formation of this widespread ... low amount of the C14 dialdehyde is probably a result of instability The formation of b-ionone from b-apo-8¢-carotenal (C30) and b-apo10¢-carotenal (C27) suggested that the cleavage of the C9–C10 ... 741 A novel route for geranial formation A Ilg et al tra with the NIST library HPLC analyses of the corresponding cell pellets revealed the accumulation of a complex mixture of compounds, tentatively...

Ngày tải lên: 07/03/2014, 03:20

... Fernandez-Montalvan et al Biochemical characterization of USP7 Fig Enzymatic characterization of USP7 (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC (j), SUMO-1-AMC (d) and Nedd8-AMC ... inhibitor of jB kinase, a regulatory role can be presumed for USP7 phorsphorylation The identification of K869 as the ubiquitination site of USP7 represents additional evidence for the interaction of ... Institutes for Biomedical Research (NIBR) Journal compilation ª 2007 FEBS 4261 ´ ´ A Fernandez-Montalvan et al Biochemical characterization of USP7 Table Kinetic parameters for the hydrolysis of Ub-AMC...

Ngày tải lên: 07/03/2014, 05:20

... trans conformers of their substrates [22,23] Taking the cis ⁄ trans content of Bip-Pro (78% ⁄ 22%) into account, we obtained Ki trans values for Bip-Pro of 5.2 lm in Caco-2 cells and of 0.75 lm ... cells For further functional characterization of Bip-[3H]Pro uptake we therefore used SKPT cells Fig Substrate saturation kinetics of Bip-Pro and Gly-Sar transport in SKPT cells (A) Uptake of Bip-[3H]Pro ... substrate for peptide transporters Table Inhibition constants (Ki) of different substrates for the inhibition of Bip-[3H]Pro and [14C]Gly-Sar uptake in SKPT cells Uptake of Bip-[3H]Pro (4 nM, h) or of...

Ngày tải lên: 07/03/2014, 05:20