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... represents one of the wings of the goddess The designer Caroline Davidson received $35 for her work By the end of the 1970s Nike's sales reached $270million and Nike was at the centre of the fitness ... television channels the ugly amphibian discussion of the phenomenon Jamster is rubbing its hands all the way to the bank The more he irritates the grown-ups, the more the teens in the target market ... records Bill Gates, the co-owner of Microsoft is a software mxxxxxx A lot of the people who had bought sxxxxx in the company lost their money Business characteristics The success of easyJet is based...

Ngày tải lên: 05/10/2012, 09:48

... independent of the thermodynamic and kinetic properties of the binding process and is solely based on the detectability of the peptides of interest We have used this method to show that binding of fosfomycin ... differences in the thermal/vibrational content of the complex As the structure of the binary complex of MurA with bound UDPNAG is not available, it is not possible to calculate how much of the heat ... ligand binding The loop region of the upper domain moves towards the active site of the enzyme, thus shielding the substrates (and ligands) from bulk solvent A detailed thermodynamic study of this...

Ngày tải lên: 19/02/2014, 13:20

... contrast to the CD11b I domain GST, the majority of the CD11a I domain GST was found in the insoluble fraction of the E coli lyzate Modification of the solubilization conditions and increasing the volumes ... SDS/PAGE The preparations contained the expected recombinant proteins and the purity of the proteins was >90% (not shown) Binding of red cells to purified I domains The surface expression of ICAMs ... binding sites for ICAM-1 and ICAM-2 Further proof for the interaction of ICAM-4 with these I domains was obtained by comparing the ability of recombinant I domain fusion proteins to support the...

Ngày tải lên: 20/02/2014, 11:20

... in the binding of the ligand to the other repeats that share a common epitope Other inhibition mechanisms cannot be excluded; among these, there is the possibility that, through the effect of the ... compared with the binding of 15E11 to the intact repeat region (Fig 6C,D) The residual binding of 15E11 to FnBRAD9,10 could be a consequence of the weak binding of repeats and to the antibody ... FnBRs of FnBPB A panel of mouse mAbs was produced against the recombinant repetitive region of FnBPB Analysis of mAbs binding to the recombinant FnBR indicated the generation of two categories of...

Ngày tải lên: 06/03/2014, 22:21

... Panel shows the distribution of fapb6 transcripts in the distal region of the intestine (A), liver (B), ovary (C) and the adrenal homolog of the fish kidney (D) Panel shows the locations of the distal ... in the distal region of the intestine, the tissue homologous to the mammalian ileum The presence of fabp6 transcripts suggests that Fabp6 may well play a role in the uptake of lipids from the ... localize the rat Fabp6 protein and in situ hybridization to localize Fabp6 transcripts to the enterocytes of the ileum, luteal cells of the ovary and a subpopulation of steroid endocrine cells of the...

Ngày tải lên: 07/03/2014, 06:20

... GM2 and the interaction of the GM2AP ⁄ GM2 complex with the enzyme To delineate in detail the separate effect of the introduced mutations on the lipid- and the enzyme-binding of this cofactor, ... that of the WT protein Only these variants of GM2AP were examined in all further studies To assess the potential of the variant GM2AP forms for the stimulation of HexA catalysed hydrolysis of GM2, ... [16,17] Several lines of evidence indicate that GM2AP interacts with the a subunit of HexA, but the presence of the b subunit enhances binding [5,16,18,19] The crystal structure of mature GM2AP expressed...

Ngày tải lên: 07/03/2014, 12:20

... phosphorylation of these sites might affect the DNA binding and/or dimerization activities of dHAND We first analyzed the effect of phosphorylation on the transcriptional activity of dHAND using ... detect the effects of Akt phosphorylation on dimerization activity of dHAND, we next examine whether the DNA binding activity of dHAND could be affected by Akt phosphorylation The influence of phosphorylation ... absence of E47 But in the presence of E47, the expression level of this mutant was equivalent to wild type dHAND or other mutants (Fig 6B, lower panel) This indicated the transcriptional activity of...

Ngày tải lên: 07/03/2014, 16:20

... impaired in binding of the R13 region Both of these patterns contributed to the loss of binding contacts of mutant 72 Construction of a DA HbpR derivative In order to study whether the A-domain ... larger than the sequences of the palindromes [19,21], but the exact contribution of nucleotides in the binding site contacted by the proteins is not known The current hypothesis of hexameric ... in other figures Sequence numbers refer to the locations of the transcriptional start site of hbpC (B) Alignments of the sequences of three HbpR-binding sites within the hbp gene region [19] The...

Ngày tải lên: 07/03/2014, 17:20

... solubilized the protein fragment in 10 mm CHAPS buffer The combination of these two A Dynamic structure of XPC-binding domain of hHR23B procedures markedly improved the quality of the 15NHSQC ... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the structures of these ... similar to other well-refined regions, in spite of the presence of internal motion (si) and a remarkable exchange process (Rex) The NOE values combined with the presence of internal motion and the exchange...

Ngày tải lên: 07/03/2014, 17:20

... side of the membrane While in the E2 form the Ca2þ binding sites have translocated across the membrane to the luminal side and are of low affinity Furthermore, in the E1 conformational state, the ... concentrations of curcumin (25 mM ) the Km for the catalytic site is also possibly increased In order to further assess the possibility of curcumin affecting the interaction of ATP binding to the ATPase, ... [32P]ATP in the absence of Ca2þ (Fig 4A) The data showed that the amount of ATP bound to the ATPase was reduced by curcumin The binding inhibition had a apparent Ki (IC50) of about mM Reversing the...

Ngày tải lên: 08/03/2014, 23:20

... (871.36), the sulfur–sulfur bond (905.36) and the sulfur–carbon bond of the open thiazoline ring of bacitracin A (937.33) Negative ion mode CID-TOF ⁄ TOF MS analysis of the same precursor yielded the ... proportion of fragments in the ion source The combination of in-source decay of the precursor m ⁄ z 2343.09 with CID on the in-source fragment m ⁄ z 937.22 in positive ion mode confirmed the location of ... making them the active antimicrobial compounds of the bacitracin mix The oxidation of the amino-thiazoline ring to the keto-thiazole ring to form bacitracin H and F results in a loss of metal binding...

Ngày tải lên: 14/03/2014, 23:20

... as the substitution of the chlorine atom with the more electronegative fluorine in the CDNB molecule did not affect the kcat values Altogether, these results are consistent with the idea that the ... Lys117 and Val172 in the GSTU2 isoform, all of the mismatches being located in the H-sites of the enzymes [11] As long as the enzymes were expressed in vitro and purified, they exhibited different ... affinity of GSTU1 for this substrate The Km values of GSTU1 and GSTU2 for CDNB are in the range of those of plant GSTs, whose values vary from 0.12 to 4.43 mm [24,29,30] As expected on the basis of the...

Ngày tải lên: 15/03/2014, 09:20

... features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic properties of the mutants, and simulations of the substrate–enzyme interactions of the wild-type ... analysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding The amino acid sequence of Zm-p60.1 was compared with the ... in the wild-type, whereas the proportions of a helices appear to be lower in F461L, F200K and P2 Furthermore, the F193A and W373K mutations not result in any change in the thermostability of the...

Ngày tải lên: 16/03/2014, 04:20

... represent the geometric centers of the cavities that are located over the protein surface, and the blue spheres indicate the geometric centers of the cavities that are located inside the b-barrel The ... indicating the importance of the positive charges for the occurrence of nonspecific contacts with DNA [22] The BPV-1 protein shows low flexibility of the loop connecting the b2 and b3 strands (Fig 6B) The ... For HPV-16, the flexibility of the loop may alter the adaptability of the DNA-binding helices, thus modulating the discrimination of specific versus nonspecific DNA sequences For both the E2s, we...

Ngày tải lên: 16/03/2014, 10:20

... Deter¨ mination of the nuclear magnetic resonance structure of the DNA-binding domain of the P22 c2 repressor (1–76) in solution and comparison with the DNA-binding domain of the 434 repressor ... stearothermophilus DnaB confirmed the importance of residues in these parts of the protein [3,16] Unlike in the wildtype protein, the individual N-terminal and C-terminal domains of B stearothermophilus ... half of them The present structure of monomeric E coli DnaG-C identifies the earlier crystal structure of the same protein as a domain-swapped dimer, in which helix of one monomer binds to the...

Ngày tải lên: 16/03/2014, 12:20

... immobilization of the motion In the weak binding state of F-actin–HMM ⁄ AMPPNP and F-actin– HMM ⁄ ADP ⁄ AlF4 complexes the motion of the probe is less restricted relative to the strong binding state and there ... orientation of the probe molecules The disorder can arise either from disorder of a local region of the protein or from improper orientation of the filaments X-Ray diffraction experiments led to the conclusion ... concentrations of added HMM To further study the flexibility of the filaments, the mobility of the paramagnetic probe attached rigidly to the filaments was determined at different temperatures (Fig 5) The...

Ngày tải lên: 16/03/2014, 13:20

... determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found A theoretical prediction of the secondary structural organization of Ag-NPA-1 performed on the basis of the amino acid ... in cells of the inner hypodermis and the epithelium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms Discussion A bundle of four a-helices constitutes the secondary ... fluorescence of Ag-NPA-1 by acrylamide A value of 1.3 M)1 was calculated for the KQ constant Fig Binding of oleic acid, followed by the enhancement of the Trp fluorescence (DF) The curve fits best the experimental...

Ngày tải lên: 16/03/2014, 18:20

... tested The binding of both MoAb 15 and MoAb 44 to the b-chain was Ó FEBS 2003 The role of CCP2 of the C4BP b-chain in binding of protein S (Eur J Biochem 270) 97 Fig SDS/PAGE analysis of recombinant ... I16) [12] Binding assay In an attempt to define the epitopes for the MoAb 15 and MoAb 44, they were tested for binding of the different recombinant b-chain CCP1-2 constructs Two of the b-chain ... four different binding assays using microtiter plates were used (a) First, the effect of increasing amounts of fluid phase protein S on the binding of the MoAbs to immobilized recombinant b-chain...

Ngày tải lên: 17/03/2014, 09:20

... 2002 of the inhibitor in protease binding can differ with the target protease [28] The contributions of the N-terminal region and the first binding loop of family and cystatins, as well as of the ... Leu73–Asn77 segment in the second binding loop of cystatin A After each of these steps, the conformation of the second binding loop in the complex was refined by energy minimization of the Leu73–Asn77 ... considerably decrease the contribution of the second binding loop of cystatin A to the inhibition of target proteases However, the flexibility of the second binding loop of cystatin A demonstrated...

Ngày tải lên: 17/03/2014, 10:20

... have an allocrite-binding site located on the ATP-binding domain [31] Furthermore, the ATP-binding component of the Escherichia coli ABC transporter KspTM involved in the transport of polysialic ... ions but the hydrolysis of ATP was also observed in the presence of manganese, cobalt and calcium ions (Table 1) To further characterize the ATPase activity of both GST-NBDs, the effect of ATPase ... expected to bind or transport glutathione-conjugates In comparison, we have measured the effect of S-decyl-glutathione on the ATPase activity of MalK [40] and of GlcV, the ATP-binding subunit of a glucose...

Ngày tải lên: 17/03/2014, 23:20