characterization of adult stem cells

... 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells 10 1.5.2.1.1 Hematopoietic stem cells 11 1.5.2.1.2 Mesenchymal stem cells 11 1.5.3 Bone marrow stem ... the traditional dogma of adult stem cell commitment and presents the impetus for a burgeoning interest in adult stem cell therapeutics The use of adult bone marrow stem cells in cellular transplantation ... cardiovascular research 1.5.2.1 Bone marrow stem cells There are most common types of stem cells in bone marrow: (i) hematopoietic stem cells and (ii) mesenchymal stem cells These cell types are believed...

Ngày tải lên: 12/09/2015, 09:42

... developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/ progenitor cells Stem Cells 2006, 24:889-895 NIA array analysis [http://lgsun.grc.nia.nih.gov/ANOVA/ ... Background Multiple types of adult stem cell exist, of which the hematopoietic stem cell (HSC), which gives rise to cells of all hematopoietic lineages for the life of an animal, is the best ... expression of genes known to impart pluripotency in ESCs are responsible for the broader differentiation capacity of MAPC compared with other adult stem cells The molecular characterization of MAPCs...

Ngày tải lên: 14/08/2014, 08:20

... interventions28 Mesenchymal Stem Cells In The Treatment Of Acute Myocardial Infarction With the emergence of stem cells as potential regenerative medicine, attempts to use stem cells to reduce infarct ... Mesenchymal stem cells and their potential as cardiac therapeutics Circ Res 95, 9–20 Quesenberry, P.J., Aliotta, J.M., 2008 The paradoxical dynamism of marrow stem cells: considerations of stem cells, ... CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL STEM CELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI (B.Eng (Hons.)), NTU A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY...

Ngày tải lên: 10/09/2015, 08:42

... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in...

Ngày tải lên: 23/03/2014, 07:20

... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells 13 Gene ... Differentiation of Mouse Embryonic Stem Cells Early Commitment Steps and Generation of Chimeric Mice Lineage Specific Differentiation of Mouse ES Cells: Formation and Differentiation of Early Primitive...

Ngày tải lên: 11/04/2014, 00:32

... immunohistochemical staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... 7042014 of the National Science Foundation of Beijing, China, the 50573044 of the National Natural Science Foundation of China and the 2005CB623905 of the National Basic Research Program of China ... protruding processes Immunofluorescence staining of β-tubulin, GFAP and GalC demonstrated positive expressions in a substantial number of cells, suggesting that neurosphere-derived cells were able to...

Ngày tải lên: 18/06/2014, 15:20

... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... here lies in implantation of hepatic stem cells, or in implantation of hepatic cells obtained by differentiation of a different type of stem cell, such as mesenchymal stem cells Other applications, ... are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are primarily unipotent cells, under certain conditions they show a plasticity...

Ngày tải lên: 18/06/2014, 16:20

... generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... median of the number of these cells was (range: 1-43)/10 mL blood EpCAM+CKCD45- cells were detected in only two cases The detection rate of EpCAM-CK+CD45- cells was significantly higher than of EpCAM+CK-CD45 ... remaining cells were washed with PBS containing 0.5% bovine serum albumin (BSA) and mM EDTA Cells were resuspended in this buffer at a concentration of × 108 cells/ mL The enrichment of tumor cells...

Ngày tải lên: 18/06/2014, 19:20

... generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... median of the number of these cells was (range: 1-43)/10 mL blood EpCAM+CKCD45- cells were detected in only two cases The detection rate of EpCAM-CK+CD45- cells was significantly higher than of EpCAM+CK-CD45 ... remaining cells were washed with PBS containing 0.5% bovine serum albumin (BSA) and mM EDTA Cells were resuspended in this buffer at a concentration of × 108 cells/ mL The enrichment of tumor cells...

Ngày tải lên: 20/06/2014, 03:20

... phenomenon of MSCs was confirmed in different experimental systems [2,12] Other cell types, such as umbilical cord matrix stem cells (UCMS) [14], neural stem cells [15,16] and endothelial progenitor cells ... delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells Stem Cells 2007, 25(7):1618-26 12 Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L: Mesenchymal progenitor cells ... localization advantage, compared to unmodified cells Given complexity of the processes of biodistribution and homing of injected cells, we reasoned that an effect of engineered cell targeting would be...

Ngày tải lên: 20/06/2014, 07:20

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Ngày tải lên: 07/08/2014, 18:21

... accelerated degeneration of the treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow ... E, Bertram H, Abel R, Chen B, Winter A, Richter W: Induction of intervertebral disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 44 Risbud MV, Albert TJ, Guttapalli ... by a number of groups worldwide is the use of autologous MSCs to aid in the regeneration of the IVD extracellular matrix To date, however, the optimum method of application of these cells for regeneration...

Ngày tải lên: 09/08/2014, 01:22

... mesenchymal stem cells and neural progenitor cells Haematologica 2003, 88:126-133 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells ... L-selectin)/lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts Augments B responses/ plasma cells Growth, differentiation, and isotype-switching of B cells/ B cells Ligand ... infused in the 32nd week of pregnancy After an uneventful pregnancy a bone biopsy performed at months of age demonstrated engraftment of Y chromosome positive cells (7.4% of total cells) [52] These...

Ngày tải lên: 09/08/2014, 08:23

... Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) ... 36 37 38 39 40 41 42 43 44 45 46 47 48 49 characterization of mesenchymal stem cells Stem Cells 2006, 24:475-481 Charo IF, Ransohoff RM: The many roles of chemokines and chemokine receptors in ... Figure Time-course expression of major components of the chondrocyte-associated extracellular matrix (ECM) The gene-expression profile of mesenchy(ECM) mal stem cells (MSCs; n = 2) was analysed...

Ngày tải lên: 09/08/2014, 10:20

... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently ... promotes self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth ... cannot shorten the time of the neural induction from ES cells The Sox1-GFP+ cells did not appear on differentiation day 2, regardless of the FGF1 treatment The increase of Sox1+ cells in the FGF1-treated...

Ngày tải lên: 10/08/2014, 05:21

... Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... Grünwald-Giemsa staining (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle-like cells Unlike the round-polygonal cells, HPB-AML-I cells with the spindle-like ... undifferentiated cells Inverted microscopic examination demonstrated the presence of a number of vacuoles in the cytoplasm of differentiated HPB-AML-I cells (Figure 4G) In contrast to the undifferentiated cells...

Ngày tải lên: 10/08/2014, 10:20

... ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs ... migration of mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells Stem Cells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stem cells ... C: (×400) Area of cell drop out with stem cells, D: (×400) No nodularity & liver appears normal, few collections of round to oval stem cells in lobules Page of 11 Figure Detection of MSCs labeled...

Ngày tải lên: 10/08/2014, 10:21

... may offer an experimental means of verifying the relationship of SNPs or haplotypes with diseases Abbreviations CHARGE, Coloboma of the eye, Heart defects, Atresia of the choanae, Retardation of ... human embryonic stem cells Cell 2005, 122:947-956 Cole MF, Johnstone SE, Newman JJ, Kagey MH, Young RA: Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells Genes ... expression programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human-induced...

Ngày tải lên: 11/08/2014, 12:21

... autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) ... mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10(910):1518-1525 Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse ... mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation...

Ngày tải lên: 12/08/2014, 01:22

... 7.6.1.0 software) The number of cells that migrated to the lower side of the membrane was counted by means of light microscopy, operated by an investigator who was blind to the types of sera present ... dividing the number of Page of cells counted by the mean value of counted cells in controls Endothelial wound healing assay A wound healing assay was used to investigate the effects of apneic serum ... early activation of MSC: specifically, an increase in the mobility of these stem cells and in their adhesion to endothelial cells Moreover, it was also found that the serum of apneic rats improved...

Ngày tải lên: 12/08/2014, 11:22