characterization isolation and gene expression profiling of fetal adrenal neuroblasts

... relationship studies and the molecular characterization of the fruit and flowers There has been some gene characterization of avocado fruit ripening genes [18-22] The greater part of molecular detail ... Cass LG, Kirven KA, Christoffersen RE: Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening Molecular and General Genetics MGG 1990, 223:76-86 ... pyrosequencing data and gene expression profiles of defence-related genes Sequence ID GenBank accession number cDNA Library Max qRT-PCR expression Similarities between 454 and qRTPCR Thaumatin...

Ngày tải lên: 11/08/2014, 11:21

... induce CASP9 and PIG11, while wild-type p53 in group II may induce these genes in group II The results of the study suggest that a combination of histopathology and gene- expression profiling is ... histopathogensis of synovitis in early RA Different expression profiles of the several candidate genes may provide useful information for future studies of the diagnosis and prognosis of early RA ... On the basis of these findings, we speculate that the two groups may reflect differences in the pathogenesis of synovitis The different expression profiles of several candidate genes for RA reported...

Ngày tải lên: 09/08/2014, 06:23

... cellular localization and cell death (Table 2) and for down-regulated genes included regulation of progression through cell cycle, mitotic phase of cell cycle and DNA damage response and repair (Table ... the Affymetrix Gene Arrays and for technical support and advice and Henrik Edgren at Institute for Molecular Medicine, University of Helsinki, Finland for help with the Affymetrix gene normalisation ... methods of assessment of late normal tissue injury and categorisation of patients into cases with late radiation effects and controls with minimal/none Additional file 2: Cell cycle analysis of adult...

Ngày tải lên: 09/08/2014, 09:20

... region of the proximal femur and to compare gene expression in bone from OA, control and OP individuals We identified altered expression of WNT and TGF-β/BMP signalling pathway and target genes ... TFF3, CTSG and LTB) and downregulation of a group of genes that negatively influence angiogenesis (HIG2, ADAMTS4, ANGPTL4, STC1, KLF6, IGFBP3, TIMP4 and GDF15) In addition, a subset of the genes with ... cartilage degeneration and ultimately loss of joint function) Therefore, it is to be expected that many genes and small changes in the expression of these genes would be involved in OA pathogenesis...

Ngày tải lên: 09/08/2014, 10:21

... of genes and individual candidates that are involved in the perturbation of mechanisms of iron homeostasis that results from Hfe disruption, and to identify differences in gene expression profiles ... characterized mouse genes, and bioinformatics tools to characterize expression networks in the duodenum and the liver of wild-type control and Hfe-/B6 and D2 mice Results Differential gene expression ... unexpectedly, we observed significant downregulation of the sideroflexin gene (Sfxn2) and upregulation of the mitoferrin gene (Slc25a37) and the Bcrp gene (Abcg2), which encode three molecules that...

Ngày tải lên: 14/08/2014, 08:20

... distinct (and possibly more heterogeneous) subgroup in terms of gene expression, as the Gleason tumors As the TMPRSS2:ERG gene fusion was detected in one out of two Gleason cultures tested, and is ... place of cDNA for the negative control Fold change in expression PE704 Fold change in expression PE687 Figure Validation of selected genes by quantitative real time PCR Validation of selected genes ... Figure Validation of selected genes by flow cytometry and immunocytochemistry Validation of selected genes by flow cytometry and immunocytochemistry (a) Flow cytometry analysis of prostate cancer...

Ngày tải lên: 14/08/2014, 08:21

... Fernandes J, Walbot V: Comparative profiling of the sense and antisense transcriptome of maize lines Genome Biol 2006, 7:R22 Ma J, Duncan DS, Morrow DJ, Fernandes J, Walbot V: Transcriptome profiling ... microspore and pollen (42 genes); and up-regulated in binucleate microspore and mature pollen (18 genes) Surprisingly, the majority of zinc-finger related genes are expressed throughout most of anther ... Heatmap of relative expression values for genes similar to zinc finger Heatmap of relative expression values for genes similar to zinc finger proteins Genes are sorted by the cluster generated...

Ngày tải lên: 14/08/2014, 21:20

... study to profile the expression of multiple metalloproteinases and their inhibitors in actively resorbing cartilage MMP-1, -2, -3, -9, -12, -13, and -14 and ADAMTS-4, -5, and -9 gene expression ... mechanisms of cartilage degradation (for example, [31,32]) and the efficacy of novel therapeutics (for example, [22,23,33,34]) Several studies have profiled the expression and regulation of metalloproteinases ... are the mean ± standard error of the mean = control; ▲ = IL-1+OSM PCR Furthermore, we have correlated this gene expression with gelatinase and collagenase enzyme expression and activation, as...

Ngày tải lên: 09/08/2014, 08:22

... analysis of miRNA gene expression of 41 mammary tumors derived from genetically engineered mouse models and samples of normal mammary glands from 17.5-day-pregnant FVB/N mice The heatmap shows the expression ... SIP1 and ZEB1, which are mediators of EMT Thus, expression of miR-141, -200a and -200b in luminal tumors is in keeping with maintenance of the luminal phenotype Comparison of miRNA expression of ... understand the associations between oncogenic drivers and miRNA expression in sub-types of breast cancer, we performed miRNA expression profiling on mammary tumors from eight well-characterized genetically...

Ngày tải lên: 09/08/2014, 23:20

... analysis of miRNA gene expression of 41 mammary tumors derived from genetically engineered mouse models and samples of normal mammary glands from 17.5-day-pregnant FVB/N mice The heatmap shows the expression ... SIP1 and ZEB1, which are mediators of EMT Thus, expression of miR-141, -200a and -200b in luminal tumors is in keeping with maintenance of the luminal phenotype Comparison of miRNA expression of ... understand the associations between oncogenic drivers and miRNA expression in sub-types of breast cancer, we performed miRNA expression profiling on mammary tumors from eight well-characterized genetically...

Ngày tải lên: 09/08/2014, 23:20

... adapted lines of replicate populations by utilizing gene expression profiling [17,19] Here, we examined the parallelism of metabolite and gene expression levels among the evolved lines of different ... evolution of these evolved strains, which exhibited increased fitness in growth compared with their ancestor strains Gene expression profiling Several studies have used gene expression profiling ... compared expression levels of the transcripts in all of the evolved conditions The comparison of MG/MGAdp and DH/DHAdp lines among 4,159 genes revealed that 15% (633 genes) and 19% (814 genes),...

Ngày tải lên: 14/08/2014, 08:21

... Summary of expression analyses of WRKY genes under cold, drought and salinity treatments 92 Summary of expression analyses of WRKY genes under various hormone treatments 94-95 Summary of expression ... amount of mRNAs The genome-wide profiling of gene expression or transcripts level is required for understanding gene regulatory networks High throughput analysis of differential gene expression ... OsRIP genes in panicles 73 Figure 3.10 Roots specific and more than one tissues expression pattern of OsRIP genes 74 Summary of expression analyses of OsRIP genes under abiotic, biotic and ABA...

Ngày tải lên: 14/09/2015, 14:12

... 41 genes ↑ 56 genes ↑ gene ↓ DNA-repair gene ↓ genes ↑ gene ↓ 14 genes ↑ oncogenes/tumor related genes gene ↑ 11 genes ↓ genes ↑ 11 genes ↓ 13 genes ↑ 10 genes ↓ cell adhesion genes ↑ genes ↓ genes ... system genes ↑ 13 genes ↓ genes ↑ 19 genes ↓ genes ↑ 25 genes ↓ 13 genes ↓ genes ↑ genes ↓ 18 genes ↑ 10 genes ↓ signal transduction transcription gene ↑ genes ↓ 10 genes ↑ genes ↓ 22 genes ↑ genes ... transport genes ↑ 12 genes ↓ 11 genes ↑ 15 genes ↓ genes ↑ 15 genes ↓ development gene ↑ genes ↓ genes ↑ genes ↓ 12 genes ↑ genes ↓ calcium-binding genes ↑ genes ↓ gene ↓ apoptosis unknown genes ↓ genes...

Ngày tải lên: 18/06/2014, 15:20

... changes in expression of thousands of genes [9,12,13] Another challenge is that the specificity and sensitivity of a given RNA profiling platform are affected by the abundance and variability of the ... spectrophotometry and quality was assessed using an Agilent Bioanalyzer and the Agilent RNA 6000 Nano kit (data not shown) Gene expression profiling Aliquots of each sample were profiled for gene expression ... Gathof BS, Pillai R, Zander T, Schultze JL: Comparison of different isolation techniques prior gene expression profiling of blood derived cells: impact on physiological responses, on overall expression...

Ngày tải lên: 18/06/2014, 16:20

... publication of results achieved with the current state of methodology is essential in the exchange and development of different approaches to gene expression profiling and in comparing selected candidates ... collating of information and development of molecular network models will be essential and will provide the basis for functional interpretation Current status of gene expression profiling in ... limited number of genes Concordance or divergence of results can therefore be estimated only from the selection of genes published in more detail Up to now, gene expression profiling has given...

Ngày tải lên: 09/08/2014, 01:23

... further analyses Effect of the numbers of replicates on data variability gene expression experiments Average coefficient of variation with increasing number of replicates of gene expression experiments ... phase of arthritis Expression patterns of early arthritis genes R203 Hierarchical clustering of a large number of phase-specific genes (n = 507) (Fig 4) obscured the expression pattern of a relatively ... diagnosis of RA, the large variety of cell types (cells of the immune system and of synovial joints) involved in autoimmune arthritic processes, and the extreme genetic heterogeneity of the human...

Ngày tải lên: 09/08/2014, 06:22

... and the gene expression profiles of these cells were compared to the ones of CECs obtained from the same patients before treatment The treatment resulted in differential expression of 2,133 genes ... transcriptional profiles of CECs obtained from SSc patients Finally, we compared the gene expression profiles of CECs from the two subsets of SSc patients and found that 2,303 genes were significantly ... accession numbers and F.C of expression of the genes Noteworthy is that most of these genes showed a significant change at transcription level after iloprost infusion Among genes related to apoptosis,...

Ngày tải lên: 12/08/2014, 14:22

... pituitary gland, ovary and oviduct, immediately frozen in liquid nitrogen and stored at -80° C until total RNA extraction Ducks of the second (n = 350) and third (n = 251) generations of white Liancheng ... egg index, percentage of yolk, percentage of albumen and shell strength [22] DNA isolation, RNA isolation and cDNA synthesis Genomic DNA was extracted by the phenol-chloroform method from blood ... (http://cn.expasy.org/tools/) Expression profiling To determine the tissue expression of the two type splice variants, semi-quantitative RT-PCR were carried out using total RNA from various duck tissues and a pair of primers...

Ngày tải lên: 14/08/2014, 13:21

... 2007) Phylogenetic and gene expression studies are invaluable tools that aid in our understanding of the regulation and function of conserved genes and gene families such as the TWIST gene family ... fates of duplicate genes 12 Chapter 2: Materials and Methods 16 2.1 Animal stocks and maintenance 16 2.2 Isolation of genomic DNA and total RNA 16 2.2.1 Isolation of genomic DNA 16 2.2.2 Isolation ... 3.9: Expression of zebrafish twist genesexpression during the gastrula period Figure 3.10: Expression of zebrafish twist genesexpression during the early segmentation period Figure 3.11: Expression...

Ngày tải lên: 16/10/2015, 15:38

... the expression profiling of the 19 genes using four validation cell lines (RERF-LC-MS [AD], LC1/sq [SQ], 86-2 [LC], and MS-1-L [SC]) and the normal control (MRC-9) The results of expression profiling ... the expression of RAB25 and AMY2A, and positively correlated with TGM2, MALL, and IGSF3 Gene networks and gene pathways To further understand the biological networks of the 19 genes, we next ... Quantification and validation of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared...

Ngày tải lên: 15/02/2014, 04:20