chap7 9 april 08 doc

Transplantation of skeletal myoblast in ischemic heart disease 1

Ngày tải lên : 12/09/2015, 08:16

... transplantation 3.2.4.1 Cell Survival by Y chromosome Real Time PCR 84 85 85 86 86 87 88 88 89 90 91 93 94 94 96 99 100 102 104 104 xiv 3.2.5 3.2.4.2 Cell survival by β-gal assay Heart function assay ... Serum preparation and antibody detection 2 .9. 1 Serum preparation 2 .9. 2 Antibody concentration assay 2 .9. 2.1 Flow cytometry assay to detect antibody 2 .9. 2.2 Enzyme linked immunosorbent assay (Elisa) ... Figure 3.1 Figure 3.2 Figure 3.3 112 113 114 115 116 117 118 1 19 120 122 123 124 125 126 127 128 1 29 130 131 133 135 137 138 1 39 141 142 143 144 145 vii and β-gal assay 146 Figure 3.31 Linear...

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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

Ngày tải lên : 18/02/2014, 17:20

... magniﬁcation Scale bar = 50 lm 199 0 FEBS Journal 275 (2 008) 198 8– 199 8 ª 2 008 The Authors Journal compilation ª 2 008 FEBS T Yamaguchi et al expressed in CD31-positive ECs of E9.5 embryos proper and yolk ... by two distinct pathways Proc Natl Acad Sci USA 94 , 861–866 FEBS Journal 275 (2 008) 198 8– 199 8 ª 2 008 The Authors Journal compilation ª 2 008 FEBS 199 7 A new method for mouse endothelial cell culture ... and day 40–50 ECs were used for experiments shown in B–E 199 2 FEBS Journal 275 (2 008) 198 8– 199 8 ª 2 008 The Authors Journal compilation ª 2 008 FEBS VEGF-A C Uterine ECs No treatment DaAPI Lyve-1...

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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Ngày tải lên : 24/03/2014, 04:21

... H.J., Sutherland, G.R & Antalis, T.M ( 199 9) Two novel mucin genes down-regulated in colorectal cancer identified by differential display Cancer Res 59, 4083 –40 89 14 Williams, S.J., Wreschner, D.H., ... ( 199 9) The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein Cancer Res 59, 1552–1561 38 Dent, G.A., Civalier, C.J., Brecher, M.E & Bentley, S.A ( 199 9) ... & Kim, Y.S ( 199 3) Human gastric mucin Identification of a unique species by expression cloning J Biol Chem 268, 58 79 5885 10 Bobek, L.A., Tsai, H., Biesbrock, A.R & Levine, M.J ( 199 3) Molecular...

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cancer cell culture, methods and protocols

Ngày tải lên : 10/04/2014, 11:11

... 17p13.3 2pter 12q22-q24.2 15 31–34 16 70 31 30 522 90 9 600–850 400 94 0 168– 1080 600 to >5000 370–760 80a 81b 85c 78d 97 e 78f 10 to >15000 >99 .9g Heterozygosity (%) Minisatelite VNTR Apo-B1 Col2A1 ... Manoir, S., Veldman, T et al ( 199 6) Multicolor spectral karyotyping of human chromosomes Science 273, 494 – 497 28 Henegariu, O., Heerema, N A., Bray-Ward, P et al ( 199 9) Colour-changing Karyotyping: ... contamination Science 184, 1 093 –1 096 Nelson-Rees, W A., and Flandermeyer, R R ( 197 6) HeLa cultures defined Science 191 , 96 98 Nelson-Rees, W A., Daniels, D W., and Flandermeyer, R R ( 198 1) Cross-contamination...

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cell and tissue culture

Ngày tải lên : 10/04/2014, 11:12

... Metabolism PROCEDURE:Amino acid analysis Case study References 83 85 86 87 88 88 90 91 92 76 76 77 77 78 80 80 93 94 94 95 96 97 98 100 101 106 107 Vlll 3.5 3.6 3.7 CELL AND TISSUE CULTURE Tissue Culture ... 293 WCRE/ WCRIP Urlaub et al ( 198 6) Smith et al ( 198 5) Gluzrnan ( 198 1) Torado & Green ( 196 3); Torado et al ( 196 5) Gey et al ( 195 2) Halpern & Coffman ( 197 2); Lundblad et al ( 197 2); Oi et al ( 198 3) ... Recombinant cells Cell characterization studies References 277 278 280 282 282 285 288 290 290 293 295 296 297 298 299 CONCLUDING REMARKS Productivity 301 301 APPENDIX k TERMINOLOGY Some aspects of...

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introduction to cell and tissue culture

Ngày tải lên : 11/04/2014, 09:48

... previously cultured in vitro (Li et al., 199 6; Loo et al., 198 9; Roberts et al., 199 0) or the maintenance of a complex phenotype in vitro (Li et al., 199 5) continue to inform us of the cell's needs, ... Cells 89 Just Look at the Dish 89 The Light Microscope Level 89 Phase Contrast 91 Hoffman or Nomarski Optics 95 Care and Handling of the Phase Contrast Microscope 96 Fluorescence Microscopy 97 Labeling ... as Industrial Property 195 196 Engineering Cells for Specific Properties 196 Preparing, Characterizing, and Storing Cell Banks 197 Very Small Scale 198 Very Large Scale 198 References 204 Glossary...

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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf

Ngày tải lên : 18/06/2014, 15:20

... Complexity index (μm-1) Non-CD CD p 40.15 ± 5.15 91 .93 ± 15.43 88. 79 ± 22.52 0.13 ± 0.02 1.06 ± 0.16 46. 79 ± 8. 09 113.30 ± 19. 11 122.86 ± 19. 11 0.12 ± 0.02 0 .93 ± 0.11 0.025 0.0064 0.0018 ns 0.033 Feret ... 2002, 1 591 : 69- 74 Neaud V, Rosenbaum J: A red wine polyphenolic extract reduces the activation phenotype of cultured human liver myofibroblasts World J Gastroenterol 2 008, 14:2 194 -2 199 Mishra ... Nutr Clin Care 2005, 8:54- 69 Page of (page number not for citation purposes) Journal of Translational Medicine 20 09, 7:40 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Oberhuber...

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báo cáo hóa học: "Fibrillar beta-amyloid peptide Aβ1–40 activates microglial proliferation via stimulating TNF-α release and H2O2 derived from NADPH oxidase: a cell culture study" doc

Ngày tải lên : 19/06/2014, 22:20

... possible inflammatory mechanism of neuronal damage in Alzheimer's disease J Biol Chem 199 9, 274:15 493 -15 499 Sharpe MA, Ollosson R, Stewart VC, Clark JB: Oxidation of nitric oxide by oxomanganese-salen ... Histochemistry 199 4, 102:483-486 Marty S, Dusart I, Peschanski M: Glial changes following an excitotoxic lesion in the CNS – I Microglia/macrophages Neuroscience 199 1, 45:5 29- 5 39 Davies MH, Eubanks ... deposits of Alzheimer disease J Neuroimmunol 198 9, 24:173-182 Dickson DW: The pathogenesis of senile plaques J Neuropathol Exp Neurol 199 7, 56:321-3 39 Zekry D, Epperson TK, Krause KH: A role for...

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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps

Ngày tải lên : 10/08/2014, 05:21

... receptor, CC-CXR-5 Nature 199 6, 381:667-673 Dittmar MT, McKnight A, Simmons G, Clapham PR, Weiss RA, Simmonds P: HIV-1 tropism and co-receptor use Nature 199 7, 385(6616): 495 -6 Delobel P, Sandres-Sauné ... 2 008, 24 (9) :1215-1220 Wagner TA, Frenkel LM: Potential limitation of CCR5 antagonists: drug resistance more often linked to CXCR4-utilizing than to CCR5-utilizing HIV-1 AIDS 2 008, 22(17):2 393 -5 ... V75I, F77L, F116Y, Q151M, M184V/I) and NNRTI (V90I, V108I, Y181C, Y188L, M184I) and had one or more drug specific mutations, K103N, Y181C and G 190 A in the RT region HIV disease progression is...

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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx

Ngày tải lên : 11/08/2014, 00:22

... Health 199 8, 7:371-378 van der Straten A, King R, Grinstead O, Serufilira A, Allen S: Couple communication, sexual coercion and HIV risk reduction in Kigali, Rwanda AIDS 199 5, 9: 935 -94 4 Balzarini ... global overview Obstet Gynecol Clin North Am 199 7, 24:705-7 29 Elias CJ, Coggins C: Female-controlled methods to prevent sexual transmission of HIV AIDS 199 6, 10(Suppl 3):S43-S51 10 Brown H: Marvellous ... Infect Dis 2 008, 8:685- 697 12 Pauwels R, De CE: Development of vaginal microbicides for the prevention of heterosexual transmission of HIV J Acquir Immune Defic Syndr Hum Retrovirol 199 6, 11:211-221...

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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt

Ngày tải lên : 11/08/2014, 20:21

... 48:117-128 19 Arnold S, Plate U, Wiesmann HP, Stratmann U, Kohl H, Hohling HJ: Quantitative electron spectroscopic diffraction analyses of the crystal formation in dentine J Microsc 199 9, 195 (Pt 1):58-63 ... bone: structure-function relations J Struct Biol 199 9, 126:241-255 Boskey AL: Musculoskeletal disorders and orthopedic conditions Jama 2001, 285:6 19- 623 Meyer U, Wiesmann HP: Bone and cartilage ... Jones PL: Extracellular matrix and integrin signalling: the shape of things to come Biochem J 199 9, 3 39( Pt 3):481-488 27 Naito J, Kaji H, Sowa H, Hendy GN, Sugimoto T, Chihara K: Menin suppresses...

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Báo cáo khoa học: " Thermal stability and inactivation of hepatitis C virus grown in cell culture" ppsx

Ngày tải lên : 12/08/2014, 04:21

... calculated as 0.0 89- log/ and 0.1 19- log/min, for HCVcc in culture medium (A) and human serum (C), respectively, after the data were fit to a linear regression model (R2 = 0 .98 83 and 0 .98 13, respectively) ... treatments for hepatitis C Lancet Infect Dis 20 09, 9: 108- 117 Alter MJ: The detection, transmission, and outcome of hepatitis C virus infection Infect Agents Dis 199 3, 2:155-166 Kamili S, Krawczynski K, ... T, Chisari FV: Robust hepatitis C virus infection in vitro Proc Natl Acad Sci USA 2005, 102 :92 94 -92 99 13 Akazawa D, Date T, Morikawa K, Murayama A, Miyamoto M, Kaga M, Barth H, Baumert TF, Dubuisson...

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Báo cáo y học: "Calcium deposition in osteoarthritic meniscus and meniscal cell culture" pps

Ngày tải lên : 12/08/2014, 12:20

... 198 2, 49: 495 - 499 Mitrovic DR, Stankovic A, Iriarte-Borda O, Uzan M, Quintero M, Miravet L, Kuntz D: The prevalence of chondrocalcinosis in the human knee joint An autopsy survey J Rheumatol 198 8, ... mineralization and, when constitutively expressed, blocks endochondral and intramembranous ossification in the limb J Cell Biol 199 9, 147:1 097 -1 108 Theocharis AD, Seidel C, Borset M, Dobra K, Baykov ... 243.3 39 years, F 33.8 ± 15.3 173.5 ± 26.2 49 years, F 53.5 ± 32.2 882.0 ± 151 .9 43 years, M 27.5 ± 5.1 170.0 ± 25.3 50 years, F 56.8 ± 22.8 1,537.0 ± 376.3 65 years, F 27.3 ± 2.5 1, 008. 0 ± 198 .6...

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Báo cáo y học: "Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts" pdf

Ngày tải lên : 12/08/2014, 12:20

... 198 9, 9: 105-113 13 Firestein GS, Echeverri F, Yeo M, Zvaifler NJ, Green DR: Somatic mutations in the p53 tumor suppressor gene in rheumatoid arthritis synovium Proc Natl Acad Sci 199 7, 94 :10 895 -1 090 0 ... fibroblasts Arthritis Rheum 199 9, 42:1 594 -1600 15 Muller-Ladner U, Kriegsmann J, Gay RE, Gay S: Oncogenes in rheumatoid arthritis Rheum Dis Clin North Am 199 5, 21:675- 690 16 Kraan MC, Haringman ... destruction? Ann Rheum Dis 199 3, 52(Suppl 1):S 39- S47 Muller-Ladner U, Gay RE, Gay S: Molecular biology of cartilage and bone destruction Curr Opin Rheumatol 199 8, 10:212-2 19 Firestein GS: Invasive...

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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx

Ngày tải lên : 13/08/2014, 23:20

... linear segment Second linear segment Two linear segment spline Single exponential 0 .99 850 0 .99 888 0 .99 9 59 0 .99 935 ciated with the legend of Figure and Figure should be associated with the legend ... Biophys J 196 9, 9( 6): 792 -8 09 Kubitschek HE: Evidence for the generality of linear cell growth J Theor Biol 197 0, 28(1):15- 29 Kubitschek HE: Bilinear cell growth of Escherichia coli J Bacteriol 198 1, ... http://www.tbiomed.com/content/3/1/10 Human growth 80 70 60 M 50 R M R 40 30 20 Feb-86 Apr-88 Jul -90 Sep -92 Nov -94 Feb -97 Apr -99 Jun-01 Aug-03 Nov-05 Jan -08 Date of measurement Figure Chart of3height of two individuals Chart...

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Cell isolation and expansion using dynabeads

Ngày tải lên : 22/10/2014, 17:44

... (2002) Regulation of human cell engraftment and development of EBV- 68 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 A.A Neurauter et al related lymphoproliferative disorders in Hu-PBL-scid mice ... [7481] [76, 7884] [85] [85] [86 89] [90 96] [97 100] [101] [102104] [1051 09] [110115] [116, 117] [118] [1 191 21] [122] Human regulatory T cells (CD4+CD25+) Human B cells (CD 19+ ) Human NK cells (CD56+) ... T, Thompson CB, June CH ( 199 7) Effects of CD28 costimulation on long-term proliferation of CD4+ T cells in the absence of exogenous feeder cells J Immunol 1 59: 592 1 593 0 36 Carroll RG, Riley JL,...

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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka

Ngày tải lên : 14/09/2015, 13:38

... and Stat5B α and β (Schindler et al., 199 2; Caldenhoven et al., 199 6; Wang et al., 199 6; Schaefer et al., 199 7; 199 6; Moriggl et al., 199 6; Sasse et al., 199 7) The alternatively spliced RNAs from ... controlling pluripotency: Oct4, Nanog and Sox2 (Okamato et al., 199 0; Rosner et al., 199 0; Schöler et al., 199 0; Yuan et al., 199 5; Niwa et al., 2000; Mitsui et al., 2003; Chapter I Introduction ... DNA sequence 5'-ATGCAAAT-3' (Okamato et al., 199 0; Rosner et al., 199 0; Schöler et al., 199 0) Later, Oct4 also was found in human (Takeda etal., 199 2).The gene in human and mice has been designated...

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Phytohormones on cell growth and taxol accumulation via cell culture of taxus wallichiana zucc

Ngày tải lên : 23/10/2015, 15:38

... taxane biosynthesis (Menhard et al., 199 8; Yukimune et al., 199 6, 2000; 29 Ketchum et al., 199 9; Phisalaphong and Linden, 199 9; Mirajilili and Linden, 199 6) Jasmonic acid (JA) in 100 M concentration ... sp callus culture in 198 9 (Christen, 199 1) Between 199 1 and 199 6 approximately 75 papers including 18 patents were published on Taxus sp cell and tissue culture (Jaziri, 199 6) Variability in growth ... 199 4; Furuya et al., 198 4; Franklin and Dixon, 199 4; Furuya, 198 8), rosmarinic acid by Coleus bluemei (Ulbrich et al., 198 5), shikonin by Lithospermum erythrorhizon (Takahashi and Fujita, 199 1),...

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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

Ngày tải lên : 05/09/2013, 09:08

... polymer 2.5 12.4 79. 6 19. 6 67.1 3.2 0.8 26.1 20.5 55.5 4.3 0.8 4.2 9. 6 41.1 5.4 1.7 1.7 2.7 34.0 6.4 1.7 1.7 2.7 31.8 7.2 0.8 1.7 2.0 32.7 8.2 1.7 1.7 3.4 36.6 9. 3 0 .9 0 .9 0.0 26.5 9. 9 0.8 0.8 0.0 ... al ( 198 6) extracted a flocculant from the culture filtrate of Rhodococcus erythopolis, and its aggregation activity was significantly increased by cations such as Ca2+ Dermlim et al ( 199 9) also ... Vol.54, 484-4 89 Dermlim, W., Prasertsan, P and Doelle, H ( 199 9) Screening and characterization of bioflocculant produced by isolated Klebsiella sp Appl Microbiol Biotechnol., Vol.52, No.5, 698 -703 Hall,...

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Personality Traits and Workplace Culture

Ngày tải lên : 26/09/2013, 14:10

... hội, văn hóa…) Quản trị: Trần Quốc Thành, Toán, P.HT THPT Trần Đại Nghĩa, Đắk Lắk Phone: 090 59 00 99 , mail: ngoclinhson@gmail.com, Y!M: ngoclinhson Keywords: thư viện giáo dục, lý luận, phương ... will be satisfied with his or her work +++++++ Nguồn: St Helens College (ac.uk), ngày download: 09/ 2010 +++++++ NGỌC LINH SƠN THƯ VIỆN ĐIỆN TỬ, THƯ VIỆN GIÁO DỤC TỔNG HỢP http://ngoclinhson.violet.vn,...

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