cells cancer stem cells and hepatocellular carcinoma

... HCC cells- reactive T cells are induced by fusion cells, T cells from healthy donors were stimulated by fusions of DCs from the same healthy donors (HLA-A2+) and the HCC cells (HLA-A2+, WT1+, and ... nonadherent cells were removed and the adherent cells were cultured overnight To generate immature DCs (DCs), the nonadherent and loosely adherent cells were collected on the next day and placed ... penicillin, and 0.1 mg/ml streptomycin DCs alone, the HCC cells alone, an unfused mixture of both DCs and the HCC cells were used as controls T cells were purified with nylon wool and cultured...

Ngày tải lên: 18/06/2014, 15:20

... fibrosis leading to cirrhosis and hepatocellular carcinoma: a case report Journal of Medical Case Reports 2011 5:160 Submit your next manuscript to BioMed Central and take full advantage of: • ... contributions MRG had the initial idea for this work and was responsible for writing the manuscript AHG handled the patient data acquisition and the literature research MB contributed to the editing ... g/dL, prothrombin time 16.1 seconds and her international normalized ratio 1.44 She was removed from the transplantation waiting list and was admitted for staging and planning treatment strategies...

Ngày tải lên: 11/08/2014, 00:23

... characterized miRNAs and is implicated in the tumorgenesis of several types of human cancers including B-cell lymphoma, breast cancer, colon cancer, lung cancer and pancreatic cancer Our data from ... HBsAg expression in (A) PLC/PRF/5 cells and (B) 2.2.15 cells …………………………………… 89 Figure 4.4 Effect of siRNA on HBsAg RNA in (A) PLC/PRF/5 cells and (B) 2.2.15 cells ……………………………………………………………… 91 ... might aid in cancer diagnosis and understanding of miRNA function in cancers may provide therapeutic targets Keywords: Short interfering RNA, microRNA, hepatocellular carcinoma, hepatitis B, expression...

Ngày tải lên: 14/09/2015, 12:11

... cells, and a novel approach for the identification of potential targets in the diagnosis and treatment of human hepatocellular carcinoma Acknowledgments We are grateful to Dr Rolf K-H Kinne and ... the identification of potential targets in the diagnosis and treatment of human hepatocellular carcinoma Materials and methods Cell culture and reagents Based on the reported alteration of gene ... and doxorubincin in human hepatocellular carcinoma derived cell lines The resistance against anticancer drugs has been associated with the expression of transcription factors, such as NFκB and...

Ngày tải lên: 31/10/2012, 15:28

... c-glutamylcysteine synthetase [14] and glutathione transferase [15] in cancer cells Such alterations of the glutathione system can enhance survival of cancer cells In general, hypoxia and reoxygenation may ... antioxidant system, in particular glutathione metabolism, and on studying DNA damage The metabolism of glutathione and DNA breaks were investigated in hepatocellular carcinoma HepG2 cells cultivated ... HepG2 cells during hypoxia and after reoxygenation HepG2 cells were incubated for h under normoxic and hypoxic conditions in the absence and presence of pyruvate (0.8 mm) Thereafter, hypoxic cells...

Ngày tải lên: 18/02/2014, 16:20

... lower levels of uPA and c-met proteins and mRNAs were detectable in AB2 normal cells and in the differentiated HCC cells, HepG2 and HuH6 cells (Fig 2A,C) These data Fig The uPA and c-met 3¢-UTRs, ... uPA and c-met protein expression in AB2 cells Western blot analysis (A) of uPA in the conditioned media of control cells (lanes 1, 2, 4, and 5) and anti-miR-23b-transfected cells (lanes and 6) ... total RNA isolations from control cells (lanes 1, 2, 4, and 5) and transfected cells (lanes and 6) were examined by RT-PCR to detect uPA mRNA (A) and c-met mRNA (B), and by real-time PCR to detect...

Ngày tải lên: 29/03/2014, 23:20

... CD44, CD24, ESA and ALDH1 in different cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stem cells and cancer stem cells are known ... overexpressed ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population ... Batra SK: Ovarian cancer: emerging concept on cancer stem cells J Ovarian Res 2008, 1:4 Dean M, Fojo T, Bates S: Tumour stem cells and drug resistance Nat Rev Cancer 2005, 5:275-284 Andrianifahanana...

Ngày tải lên: 20/06/2014, 07:20

... Telmer and Eva Turley Breast Cancer Stem Cells 235 Chapter 11 The Microenvironment of Breast Cancer Stem Cells Deepak Kanojia and Hexin Chen Chapter 12 Involvement of Mesenchymal Stem Cells in ... of breast cancer cells, molecular tumor classification methods, in vitro cancer models, breast cancer and microenvironment, breast cancer stem cells, gene regulation in breast cancer, and mechanism ... Muehlberg, F., Beckmann, C and Song, Y H (2009) Tissue resident stem cells produce CCL5 under the influence of cancer cells and thereby promote breast cancer cell invasion Cancer Lett, Vol 284, No.1:...

Ngày tải lên: 27/06/2014, 17:20

... costunolide for 0, 2, 4, 16 and 24 hours) Then the cells were harvested and washed with phosphate buffered saline (PBS), then fixed and permeated at 4°C for hour with 70% ethanol Cells were stained for ... hepatoma cells and human normal macrophages treated by costunolide Cell viability was assessed by trypan blue exclusion test for HA22T/VGH cells and MTT assay for macrophages A, HA22T/VGH cells ... expression of protein assay YJC and SLF both conceived of the study, and participated in its design and coordination and helped to draft the manuscript All authors read and approved the final manuscript...

Ngày tải lên: 09/08/2014, 09:20

... expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells Mol Cancer 2007, 6:75 19 Pfeiffer MJ, Smit FP, Sedelaar JP, Schalken JA: Steroidogenic enzymes and stem cell markers ... AK1C3 [19] and CD44 [20] have been proposed to be the markers of cancer stem cells Their expression in tumor cells could correlate with their aggressive biological behavior, drug resistance and poor ... variety of malignant tumor cells In particular, AK1C1, and AK1C3 are reported in human non-small cell lung carcinoma (A549) cells [21], and a high expression of AL1A1 in lung cancer cell lines, especially...

Ngày tải lên: 10/08/2014, 09:22

... secretion of BDNF in HepG2 and HCCLM3 cells by ELISA BDNF is a cytokine secreted by a few human cancers, supporting growth and survival of tumor cells [23] To explore whether HCC cells express BDNF secretorily, ... that both anti-BDNF and K252a effectively interrupted the invasion of HepG2 and HCCLM3 cells Discussion Hepatocellular carcinoma is the most lethal malignancy in many countries, and the incurable ... cotton tip, and the migratory cells attached to the lower membrane surface were fixed with 4% paraformaldehyde and stained with crystal violet The number of migrated cells was counted in randomly...

Ngày tải lên: 10/08/2014, 10:21

... Stem Cells to Overcome Drug Resistance 401 Miaorong She and Xilin Chen Chapter 21 Cancer Stem Cells and Chemoresistance Suebwong Chuthapisith Chapter 22 Cancer Stem Cells in Drug Resistance and ... Markers and Targets for New Combination Therapies Candace A Gilbert and Alonzo H Ross Chapter 79 Cancer Stem Cells in Lung Cancer: Distinct Differences between Small Cell and Non-Small Cell Lung Carcinomas ... Chapter Prostate and Colon Cancer Stem Cells as a Target for Anti -Cancer Drug Development Galina Botchkina and Iwao Ojima Niches and Vascularization 135 155 Importance of Stromal Stem Cells in Prostate...

Ngày tải lên: 12/08/2014, 06:20

... normal stem cells to cancer stem cells Adult normal stem cells represent one purported origin of cancer stem cells What causes the transformation from normal stem cells to cancer stem cells? ... checkpoint activation and apoptosis in cancer stem cells Another possible reason for increased genomic instability in cancer stem cells is malignant transformation of aged stem cells Stem cells have a ... Clarke, M.F., and Weissman, I.L (2001) Stem cells, cancer, and cancer stem cells Nature 414, 105-111 Ringrose, L., and Paro, R (2004) Epigenetic regulation of cellular memory by the Polycomb and Trithorax...

Ngày tải lên: 12/08/2014, 06:20

... [62] Murine and human hematopoietic stem cells [63-64], murine neural stem cells [65], normal and malignant human mammary stem cells [66], and normal and malignant human colorectal stem cells [62,67] ... normal neural stem cells and glioma stem cells, and may identify new CD133- glioma stem cells 4.4 New markers: Podoplanin and Integrin Alpha There are two new promising cancer stem cell markers ... factors For instance, Wnt ligands are produced and released from both stem cells and niche cells, BMP and Shh are released from niche cells and epithelial cells respectively, and Notch signaling is...

Ngày tải lên: 12/08/2014, 06:20

... 126 Cancer Stem Cells Theories and Practice Table Cancer stem cell markers in small cell lung carcinoma amd non-small cell lung carcinoma This is trial version www.adultpdf.com Cancer Stem Cells ... differentiation and promotes a stem cell-like phenotype in human colorectal carcinoma cells Br J Cancer 101(2):320-326 Kondo T Stem cell-like cancer cells in cancer cell lines Cancer Biomark 2007;3(4-5):245-50 ... 118 Cancer Stem Cells Theories and Practice Fig Expression of ALDH1, ALDH2 and CD133 mRNA in immortalized human airway cell and lung cancer cell lines Levels of mRNA of ALDH1, ALDH2 and CD133 and...

Ngày tải lên: 12/08/2014, 06:20

... in stem cell regulation and cancer formation and progression are an attractive area of research Self-renewal of cancer stem cells Stem cells are defined by their multi-lineage differentiation and ... (Papagiannakopoulos and Kosik, 2008) According to this cancer stem cell hypothesis, cancer stem cells are tumorinitiating cells that proliferate uniquely through self-renewal Cancer stem cells are thought ... distinction between normal adult stem cells and cancer stem cells Pharmacology & therapeutics 119, 74-82 This is trial version www.adultpdf.com 16 MicroRNAs and Cancer Stem Cells in Medulloblastoma Massimo...

Ngày tải lên: 12/08/2014, 06:20

... review this issue with depth Stem cells and cancer stem cell theory Stem Cells In order to understand cancer stem cells a brief description of normal adult stem cells and the environment in which ... metastasis, and immunosurveillance avoidance in tumors This is trial version www.adultpdf.com Cancer Stem Cells as a New Opportunity for Therapeutic Intervention 381 Cancer Stem Cells Cancer Stem Cells ... lung cancer Later, In 2008, Chen et al isolated CD133+ and - cancer cell populations from lung cancer cell lines and non-small cell lung cancer patients and reported that CD133+ lung cancer cells...

Ngày tải lên: 12/08/2014, 06:20

... marker for human hematopoietic stem and progenitor cells Blood 1997, 90:5002-5012 22 Wu Y, Wu PY: CD133 as a marker for cancer stem cells: progresses and concerns Stem Cells Dev 2009, 18:1127-1134 ... cancer stem and progenitor cells Stem Cells Dev 2009, 18:17-25 Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, et al: ALDH1 is a marker of normal and malignant human mammary stem ... breast cancer Clin Cancer Res 16:45-55 19 Chen YC, Chen YW, Hsu HS, Tseng LM, Huang PI, et al: Aldehyde dehydrogenase is a putative marker for cancer stem cells in head and neck squamous cancer...

Ngày tải lên: 13/08/2014, 15:21

... primitive cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells ... signature Stem cells Stem cells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature Tumour Birnie et al R83.3 Gleason included Benign Figure Distinctive stem cell and ... interactions and stem cell self-renewal Results Identification of gene products associated with a cancer stem cell phenotype By comparing RNA expression patterns from stem and committed cells, independent...

Ngày tải lên: 14/08/2014, 08:21