... HCC cells- reactive T cells are induced by fusion cells, T cells from healthy donors were stimulated by fusions of DCs from the same healthy donors (HLA-A2+) and the HCC cells (HLA-A2+, WT1+, and ... nonadherent cells were removed and the adherent cells were cultured overnight To generate immature DCs (DCs), the nonadherent and loosely adherent cells were collected on the next day and placed ... penicillin, and 0.1 mg/ml streptomycin DCs alone, the HCC cells alone, an unfused mixture of both DCs and the HCC cells were used as controls T cells were purified with nylon wool and cultured...
... fibrosis leading to cirrhosis andhepatocellular carcinoma: a case report Journal of Medical Case Reports 2011 5:160 Submit your next manuscript to BioMed Central and take full advantage of: • ... contributions MRG had the initial idea for this work and was responsible for writing the manuscript AHG handled the patient data acquisition and the literature research MB contributed to the editing ... g/dL, prothrombin time 16.1 seconds and her international normalized ratio 1.44 She was removed from the transplantation waiting list and was admitted for staging and planning treatment strategies...
... characterized miRNAs and is implicated in the tumorgenesis of several types of human cancers including B-cell lymphoma, breast cancer, colon cancer, lung cancerand pancreatic cancer Our data from ... HBsAg expression in (A) PLC/PRF/5 cellsand (B) 2.2.15 cells …………………………………… 89 Figure 4.4 Effect of siRNA on HBsAg RNA in (A) PLC/PRF/5 cellsand (B) 2.2.15 cells ……………………………………………………………… 91 ... might aid in cancer diagnosis and understanding of miRNA function in cancers may provide therapeutic targets Keywords: Short interfering RNA, microRNA, hepatocellular carcinoma, hepatitis B, expression...
... cells, and a novel approach for the identification of potential targets in the diagnosis and treatment of human hepatocellularcarcinoma Acknowledgments We are grateful to Dr Rolf K-H Kinne and ... the identification of potential targets in the diagnosis and treatment of human hepatocellularcarcinoma Materials and methods Cell culture and reagents Based on the reported alteration of gene ... and doxorubincin in human hepatocellularcarcinoma derived cell lines The resistance against anticancer drugs has been associated with the expression of transcription factors, such as NFκB and...
... c-glutamylcysteine synthetase [14] and glutathione transferase [15] in cancercells Such alterations of the glutathione system can enhance survival of cancercells In general, hypoxia and reoxygenation may ... antioxidant system, in particular glutathione metabolism, and on studying DNA damage The metabolism of glutathione and DNA breaks were investigated in hepatocellularcarcinoma HepG2 cells cultivated ... HepG2 cells during hypoxia and after reoxygenation HepG2 cells were incubated for h under normoxic and hypoxic conditions in the absence and presence of pyruvate (0.8 mm) Thereafter, hypoxic cells...
... lower levels of uPA and c-met proteins and mRNAs were detectable in AB2 normal cellsand in the differentiated HCC cells, HepG2 and HuH6 cells (Fig 2A,C) These data Fig The uPA and c-met 3¢-UTRs, ... uPA and c-met protein expression in AB2 cells Western blot analysis (A) of uPA in the conditioned media of control cells (lanes 1, 2, 4, and 5) and anti-miR-23b-transfected cells (lanes and 6) ... total RNA isolations from control cells (lanes 1, 2, 4, and 5) and transfected cells (lanes and 6) were examined by RT-PCR to detect uPA mRNA (A) and c-met mRNA (B), and by real-time PCR to detect...
... CD44, CD24, ESA and ALDH1 in different cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancerstem cell population Stemcellsandcancerstemcells are known ... overexpressed ovarian cancercells induce the aggressiveness of the cancercellsand enrich the cancerstem cell population It also showed that MUC4 is expressed in the ovarian cancerstem cell population ... Batra SK: Ovarian cancer: emerging concept on cancerstemcells J Ovarian Res 2008, 1:4 Dean M, Fojo T, Bates S: Tumour stemcellsand drug resistance Nat Rev Cancer 2005, 5:275-284 Andrianifahanana...
... Telmer and Eva Turley Breast CancerStemCells 235 Chapter 11 The Microenvironment of Breast CancerStemCells Deepak Kanojia and Hexin Chen Chapter 12 Involvement of Mesenchymal StemCells in ... of breast cancer cells, molecular tumor classification methods, in vitro cancer models, breast cancerand microenvironment, breast cancerstem cells, gene regulation in breast cancer, and mechanism ... Muehlberg, F., Beckmann, C and Song, Y H (2009) Tissue resident stemcells produce CCL5 under the influence of cancercellsand thereby promote breast cancer cell invasion Cancer Lett, Vol 284, No.1:...
... costunolide for 0, 2, 4, 16 and 24 hours) Then the cells were harvested and washed with phosphate buffered saline (PBS), then fixed and permeated at 4°C for hour with 70% ethanol Cells were stained for ... hepatoma cellsand human normal macrophages treated by costunolide Cell viability was assessed by trypan blue exclusion test for HA22T/VGH cellsand MTT assay for macrophages A, HA22T/VGH cells ... expression of protein assay YJC and SLF both conceived of the study, and participated in its design and coordination and helped to draft the manuscript All authors read and approved the final manuscript...
... expression profiling of cancerstem cell in human lung adenocarcinoma A549 cells Mol Cancer 2007, 6:75 19 Pfeiffer MJ, Smit FP, Sedelaar JP, Schalken JA: Steroidogenic enzymes andstem cell markers ... AK1C3 [19] and CD44 [20] have been proposed to be the markers of cancerstemcells Their expression in tumor cells could correlate with their aggressive biological behavior, drug resistance and poor ... variety of malignant tumor cells In particular, AK1C1, and AK1C3 are reported in human non-small cell lung carcinoma (A549) cells [21], and a high expression of AL1A1 in lung cancer cell lines, especially...
... secretion of BDNF in HepG2 and HCCLM3 cells by ELISA BDNF is a cytokine secreted by a few human cancers, supporting growth and survival of tumor cells [23] To explore whether HCC cells express BDNF secretorily, ... that both anti-BDNF and K252a effectively interrupted the invasion of HepG2 and HCCLM3 cells Discussion Hepatocellularcarcinoma is the most lethal malignancy in many countries, and the incurable ... cotton tip, and the migratory cells attached to the lower membrane surface were fixed with 4% paraformaldehyde and stained with crystal violet The number of migrated cells was counted in randomly...
... StemCells to Overcome Drug Resistance 401 Miaorong She and Xilin Chen Chapter 21 CancerStemCellsand Chemoresistance Suebwong Chuthapisith Chapter 22 CancerStemCells in Drug Resistance and ... Markers and Targets for New Combination Therapies Candace A Gilbert and Alonzo H Ross Chapter 79 CancerStemCells in Lung Cancer: Distinct Differences between Small Cell and Non-Small Cell Lung Carcinomas ... Chapter Prostate and Colon CancerStemCells as a Target for Anti -Cancer Drug Development Galina Botchkina and Iwao Ojima Niches and Vascularization 135 155 Importance of Stromal StemCells in Prostate...
... normal stemcells to cancerstemcells Adult normal stemcells represent one purported origin of cancerstemcells What causes the transformation from normal stemcells to cancerstem cells? ... checkpoint activation and apoptosis in cancerstemcells Another possible reason for increased genomic instability in cancerstemcells is malignant transformation of aged stemcellsStemcells have a ... Clarke, M.F., and Weissman, I.L (2001) Stem cells, cancer, andcancerstemcells Nature 414, 105-111 Ringrose, L., and Paro, R (2004) Epigenetic regulation of cellular memory by the Polycomb and Trithorax...
... [62] Murine and human hematopoietic stemcells [63-64], murine neural stemcells [65], normal and malignant human mammary stemcells [66], and normal and malignant human colorectal stemcells [62,67] ... normal neural stemcellsand glioma stem cells, and may identify new CD133- glioma stemcells 4.4 New markers: Podoplanin and Integrin Alpha There are two new promising cancerstem cell markers ... factors For instance, Wnt ligands are produced and released from both stemcellsand niche cells, BMP and Shh are released from niche cellsand epithelial cells respectively, and Notch signaling is...
... 126 CancerStemCells Theories and Practice Table Cancerstem cell markers in small cell lung carcinoma amd non-small cell lung carcinoma This is trial version www.adultpdf.com CancerStemCells ... differentiation and promotes a stem cell-like phenotype in human colorectal carcinomacells Br J Cancer 101(2):320-326 Kondo T Stem cell-like cancercells in cancer cell lines Cancer Biomark 2007;3(4-5):245-50 ... 118 CancerStemCells Theories and Practice Fig Expression of ALDH1, ALDH2 and CD133 mRNA in immortalized human airway cell and lung cancer cell lines Levels of mRNA of ALDH1, ALDH2 and CD133 and...
... review this issue with depth Stemcellsandcancerstem cell theory StemCells In order to understand cancerstemcells a brief description of normal adult stemcellsand the environment in which ... metastasis, and immunosurveillance avoidance in tumors This is trial version www.adultpdf.com CancerStemCells as a New Opportunity for Therapeutic Intervention 381 CancerStemCellsCancerStemCells ... lung cancer Later, In 2008, Chen et al isolated CD133+ and - cancer cell populations from lung cancer cell lines and non-small cell lung cancer patients and reported that CD133+ lung cancer cells...
... marker for human hematopoietic stemand progenitor cells Blood 1997, 90:5002-5012 22 Wu Y, Wu PY: CD133 as a marker for cancerstem cells: progresses and concerns StemCells Dev 2009, 18:1127-1134 ... cancerstemand progenitor cellsStemCells Dev 2009, 18:17-25 Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, et al: ALDH1 is a marker of normal and malignant human mammary stem ... breast cancer Clin Cancer Res 16:45-55 19 Chen YC, Chen YW, Hsu HS, Tseng LM, Huang PI, et al: Aldehyde dehydrogenase is a putative marker for cancerstemcells in head and neck squamous cancer...
... primitive cancercells PTL induces apoptosis in primitive cancercells Percent viability of prostate cancercellsandcells from a patient with BPH treated with increasing concentrations of PTL Cells ... signature StemcellsStemcells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature Tumour Birnie et al R83.3 Gleason included Benign Figure Distinctive stem cell and ... interactions andstem cell self-renewal Results Identification of gene products associated with a cancerstem cell phenotype By comparing RNA expression patterns from stemand committed cells, independent...