... salivary epithelial cells Cells were obtained from minor salivary glands (a–e) and the HSG cell line (f–i) Positive cells staining for cytokeratin (a) and cytokeratin 19 (b) and the absence of staining ... staining with MPO, CD20, CD3, CD45 and smooth muscle actin excluded the possibility of contamination with myeloid cells, B cells, T cells or myoepithelial cells (Figure 2d–h) The HSG cell line ... cytokeratin 20 (j) indicate the ductal epithelial origin of HSG cell lines Negative staining with myeloperoxydase (MPO) (k) and CD45 (l) excludes the possibility of contamination with myeloid cells...
Ngày tải lên: 09/08/2014, 07:20
... 91,45% lung SPC-HA Transfer 93,63% Figure cytokine staining in CD4+ T cells Intracellular Intracellular cytokine staining in CD4+ T cells CD4+ T cells from the lung or bronchial lymph nodes (BLN) ... the generation of signals involved in cell- cell as well as in cell- matrix interactions Cell- cell interactions may be direct via contact of tight junction proteins, or indirect via secreted and ... immunohistochemical stainings for the corresponding surfactant protein (C) Staining of sorted AEC II with Maclura pomifera lectin revealed high purity of isolated cells Black histogram indicates staining with...
Ngày tải lên: 12/08/2014, 15:20
Báo cáo hóa học: " Identification and classification of human cytomegalovirus capsids in textured electron micrographs using deformed template matching" pptx
... for inoculation Electron microscopy In order to examine virus-infected cells by electron microscopy, uninfected and HCMV-infected cells were harvested at 1, 3, 5, and dpi and thereafter fixed in ... in order of descending matching values beginning at the left side of the top row make the mapping of virus maturation in electron micrographs rapid, objective, reliable and easy to describe In ... allowing weighting of the norms and inner products Conclusion Monitoring the in -cell structural morphology of virus assembly helps the virologist find novel insights on how to combat the virus infection...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo nghiên cứu khoa học: "USE OF IMMOBILIZED YEAST CELL IN ALCOHOL FERMENTATION FROM MOLASSES" ppsx
... of cell biomass concentration [6] [7] Thomas’ cell counting chamber Equation is used to count the numbers of cell in biomass N = [((a/b)*400) / 0.1]*103*10n (2) Where N is the amount of cells in ... yeasty cells is 54.108 cells 3.3.Determination of optimal concentration of alginate solution on yeasty cell immobilization In this work, one-gram biomass will be mixed with various alginate concentrations ... self-supporting for finance so that the results have limited We suggest that should be study adding to complete and application in alcohol fermentation in large scale Adding studies will include...
Ngày tải lên: 22/07/2014, 03:20
Báo cáo y học: "Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo" doc
... significantly inhibited (38.47%; P < 0.05, P = 0.011) cell proliferation Effects of TXL on cell nuclear morphology Nuclear staining with DAPI was used to determine apoptosis-inducing activity of TXL in ... molecular events in TXLtreated HT-29 cells result in remarkable apoptosis process Further in vivo and in vitro studies are needed to clarify the protein interactions, thereby delineating the upstream ... Combination therapy of interferon-alpha and 5-fluorouracil inhibits tumor angiogenesis in human hepatocellular carcinoma cells by regulating vascular endothelial growth factor and angiopoietins...
Ngày tải lên: 13/08/2014, 15:21
The search for stem cell in human breast milk
... cryptic integrin-binding sites on fibrillar collagen and a laminin-5 fragment that elicits epithelial cell motility), cleave cell cell adhesion proteins (for example E-cadherin), degrade cell surface ... alveoli, ductal epithelial cells lining the ducts, as shown in Fig 1.1 and myoepithelial cells that surrounds the alveoli, forming a layer separating the ductal and alveolar epithelial cells from ... assayed in milk Epidermal growth factor Insulin Thyroxine Cortisol Luteinizing hormone-releasing hormone Prostanglandins Transforming growth factors B Demonstrated in vitro effect on growth of cells...
Ngày tải lên: 11/09/2015, 09:57
Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx
... (5.1)° in aqueous solution The Asp1 side chain carboxylic group was suggested to be involved in hydrogen-bonding interaction with either the N-terminal amino group or the Arg2 side chain amino group, ... chain NH of the preceding arginine, forming a stable heptamer ring, both in the X-ray structure and the solution structure of AII (Fig 8) Indeed, aspartate residues at the N-termini of short polypeptides ... statistics Based on the 13 structures, obtained by simulated annealing in CNS followed by refinement in explicit water using NOE distance restraints, dihedral angle restraints, bond, angles, impropers,...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: "LANGUAGE SYNTHESIS GENERATION OF GERMAN FROM CONCEPTUAL STRUCTURE: MT PROJECT IN A JAPANESE/GERMAN" pot
... labelled arcs are used in two ways: a) Binary arcs either express case relations between connected symbols or combine substructures b) Unary arcs serve as modifying tags of various kinds (logical junctors, ... CONCEPTS TO LANGUAGE In the target language oriented stage the following decisions have to be made: REFERENCING AND FOCUSSING 2, For referencing and other phenomena like focussing, the simple approach ... should be target language independent, we use frame structures in a KRL-like notation Our representation distinguishes between case scb~.mta (used to carry the meaning of actions), and concept...
Ngày tải lên: 08/03/2014, 18:20
Báo cáo khoa học: "STRUCTURE AND INTONATION IN SPOKEN LANGUAGE UNDERSTANDING*" pptx
... that particular surface structures that are induced by the specific combinatory grammar that are postulated to explain coordination in English subsume the intonational structures that are postulated ... purposes the distinction between intermediate- and intonational- phrase boundaries.) Finally, we accomplish the effect of interpolation of other parts of the tune by assigning the following polymorphic ... including marked theme and rheme expressed as two distinct intonationalAntermediate phrases are by that token unambiguous as to their information structure However, sentences like the following,...
Ngày tải lên: 08/03/2014, 18:20
Báo cáo khoa học: Re-evaluation of the function of the F420dehydrogenase in electron transport ofMethanosarcina mazei potx
... [34] Cell lysate was obtained by anaerobically harvesting trimethylaminegrown cells and resuspending in Buffer A (40 mm K2HPO4 KH2PO4, pH 7.0, mm dithioerythritol, lgặmL)1 of resazurin) containing ... nding is in line with the observation that hydrogen is a preferred intermediate in the energy-conserving electron transport chain of Ms barkeri [4] A second possibility for F420H2 oxidation in ... in cell lysates Cell lysate Methanosarcina mazei b Ms mazei DfpoA-O Ms mazei DfpoF Ms mazei b Methanosarcina barkeri b Reduction of electron Electron Electron acceptor donor acceptor (nmolặmin)1...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: "USER MODELLING, DIALOG STRUCTURE, DIALOG STRATEGY IN RAM-ANS" potx
... user initializes the dialog step "Finding out what the user wants - In the dialog phase concerning a particular room the system asks for regaining the initiative If the user denies the questioning ... represented in the conceptual network thus determining noun meaning, the meaning of "group nouns" could not be represented in the same formalism Second, inversing the extraction process and entering ... concept "Zimmerbar" (room bar) consisting of a refrigirator, drinking glasses and drinks ANSWERING QUESTIONS ABOUT THAT PARTICULAR ROOM / taking the initiative BOOKING THE ROOM (OR NOT) :GOOD-BYE Another...
Ngày tải lên: 18/03/2014, 02:20
Báo cáo khoa học: Dynamics in electron transfer protein complexes potx
... Tight-binding inhibition of angiogenin and ribonuclease-A by placental ribonuclease inhibitor Biochemistry 28, 225–230 Janin J (2000) Kinetics and thermodynamics of protein– protein interactions In ... distance restraints that can be used to determine the orientations of the two proteins in the complex RDCs are obtained by partial alignment of protein molecules resulting in incomplete averaging of ... protein–protein interactions Curr Opin Struct Biol 10, 153–159 Suh JY, Tang C & Clore GM (2007) Role of electrostatic interactions in transient encounter complexes in protein–protein association investigated...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: Structure–cytotoxicity relationships in bovine seminal ribonuclease: new insights from heat and chemical denaturation studies on variants ppt
... form in which the chains swap their N-termini (MxM) This phenomenon was later found in many proteins, and is now well known under the name of ‘3D domain swapping’ In most cases, the swapping is ... Crescenzi O, Tancredi T & Picone D (2003) Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Eur J Biochem 270, 4729–4735 Leich F, ... Comparative protein structure modeling using Modeller Curr Protoc Bioinformatics Chapter 5, Unit 34 Fiser A, Do RK & Sali A (2000) Modeling of loops in protein structures Protein Sci 9, 1753–1773...
Ngày tải lên: 22/03/2014, 17:20
Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases potx
... clostripain The molecular modelling, in conjunction with sequence analysis studies, indicates the P1 specicity determining residue as Asp229 and locates possible calciumbinding sites involved in the ... Enzyme inactivation studies Inactivation of clostripain was performed in an incubation mixture containing, in a total volume of mL at 25 C, ChC (100 lg) in 100 mM Hepes/NaOH buffer pH 7.6 was inactivated ... clostripain homologues but it is interesting to note that both forkhead and NHL domains are implicated in proteinprotein interactions [48,49] Similarly, both bacterial immunoglobulin-like domains...
Ngày tải lên: 23/03/2014, 12:20
OrganizationalStructureandPerformanceinEuropeanBanks: A Reassessment pptx
... types of institutions in the Spanish depository industry during 1986-1995 They find that mutual institutions are more non-interest cost inefficient than commercial banks, but when performing a further ... ass+*** Herfindhal +*** Log(GDP) -*** List-*** For+*** Loans/investments-*** Loan Loss/Int.earning ass+*** Herfindhal +*** Log(GDP)-*** Cost inefficiency Profit inefficiency Log(int.earning ass)-*** ... traditional intermediation, while SHVs in innovations and financial market related business After the liberalization of the financial sector in the European Union, co-operative banks have been opting...
Ngày tải lên: 29/03/2014, 09:20
Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot
... reading frame During the cloning procedure, a cysteine residue was introduced between this signal and the mature poneratoxin Surprisingly, poneratoxin expression was detected only in cells infected ... likely reinforce its hydrophobic character Confocal microscopy was used to determine the cellular localization of the recombinant poneratoxin in insect cells The toxin was observed at the cell periphery ... defined, resulting in lowered number of experimental longrange interhelical restraints In conclusion, the solution structure of synthetic poneratoxin can be modeled as two loosely interacting helices...
Ngày tải lên: 30/03/2014, 13:20
Internal governance structure of law in Vietnam (Business Law for MBA students)
... despite starting economic reforms two decades ago and increasingly reforming the state-economic sector in various ways, such as via equitisation, leasing, selling, and restructuring, Vietnamʼs ... CORPORATE GOVERNANCE IN VIETNAM : A SYSTEM IN TRANSITION 53 The Internal Governance Structure of a Shareholding Company The mandatory governance structure of a shareholding company consists of ... the Communist Party, in order to upgrade the law on business associations and create a convenient legal environment for investors in the context of international economic integration, especially...
Ngày tải lên: 31/03/2014, 14:18
Báo cáo khoa học: "A CONSIDERATION ON THE CONCEPTS STRUCTURE AND LANGUAGE IN RELATION TO SELECTIONS OF TRANSLATION EQUIVALENTS OF VERBS IN MACHINE TRANSLATION SYSTEMS" doc
... all the nouns belonging to nearby categories in the given concepts structure and find a nouns group to which we associate the translation equivalent In this manner, we can find pairs of verb and ... O (:Things) ~ / C ~ ~ obj Play: < (:Keyboard instrument) ~ ~'T/ (:Piano) ~ ~ - - ~ u ~ y( :Organ) i ~~(:String instrument) ~ (:Musical instrument) ~ o b j Do.Play: ~ ~ ~ -'
Ngày tải lên: 31/03/2014, 17:20
data structure and algorithms in java - mitchel waite
... 5, "Linked Lists," introduces linked lists, including doubly linked lists and doubleended lists The use of references as "painless pointers" in Java is explained A Workshop applet shows how insertion, ... type int and returns it: public int getInt() throws IOException { String s = getString(); return Integer.parseInt(s); } The parseInt() method of class Integer converts the string to type int A ... beginning of main(), as you can see in the bank.java listing (You don't need to worry yet about the String[] args argument in main().) The main() method creates an object of class BankAccount, initialized...
Ngày tải lên: 17/04/2014, 09:14
modeling nanoscale imaging in electron microscopy thomas vogt, wolfgang dahmen, peter binev, editors.
... proprietary rights Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Preface Imaging with electrons, in particular using scanning transmission electron microscopy ... entitled “Imaging in Electron Microscopy” in 2009 and 2010 and “New Frontiers in Imaging and Sensing” in 2011 At these workshops world-class practitioners of electron microscopy, engineers, and ... accompanied by an increase in signal to noise—resulting in higher contrast images However, in some cases the increase in beam current (that gives rise to the increase in signal to noise) results in beam...
Ngày tải lên: 30/05/2014, 00:33