cell structure and dynamics

... A and 5.5 A The ranges for backbone torsion angles / and w were derived from the values of 13Ca, 13Cb, 13C¢, 1Ha 1HN and 15N NMR structure and dynamics of eRF1 middle domain chemical shifts and ... correlation time and an order NMR structure and dynamics of eRF1 middle domain parameter between 0.5 and 1.0, against a background of faster motions occurring with a correlation time below 20 ns and an ... the most favored regions and none in the disallowed regions NMR structure and dynamics of eRF1 middle domain A B Structure analysis The conformations of the backbone and side-chains of the M...

Ngày tải lên: 07/03/2014, 05:20

... Outline  Cell theory  Properties common to all cells    why are cells so small? Prokaryotic cells Eukaryotic cells Cell size and shape –    Organelles and structure in all eukaryotic cell ... always be small Prokaryotic Cell Structure  Prokaryotic Cells are smaller and simpler in structure than eukaryotic cells   Typical prokaryotic cell is Prokaryotic cells NOT have: • Nucleus ... including cork cells  1850 – Rudolf Virchow  Proposed that all cells come from existing cells Cell Theory All organisms consist of or more cells Cell is the smallest unit of life All cells come...

Ngày tải lên: 13/03/2014, 19:31

... ‘hypervariable loop‘, D and E, E and F, F and G, and G and H are particularly tolerant of longer insertions [19] In multimodular proteins, however, insertions in the F–G and D–E loops might affect ... ellipsoidal protein CCP structures are highly Flexibility and cooperativity of CCP modules variable, with the invariant parts comprising mainly the b-strands The loops between strands B and C, known as ... folded and stable, as judged by NMR and dynamic light-scattering measurements NMR signal dispersion and secondary chemical shifts showed that the obtained proteins were properly folded and their structures...

Ngày tải lên: 15/03/2014, 23:20

... Asn12 and Lys17 (region 2) and between Lys34 and Asp39 (PAF) and Lys31 and Asp36 (AFP) (region 3) Two conserved GlyLys motifs are repeated in the structure, the glycines (i.e Gly5 from b1 and Gly20 ... indicate b-strands FEBS Journal 276 (2009) 2875–2890 ª 2009 The Authors Journal compilation ª 2009 FEBS 2877 Structure and dynamics of an antifungal protein G Batta et al b-strand secondary structure ... adjacent Lys9 and Lys39 Similarly, Asp23 stabilizes the juxtaposed Lys15 and Lys17 side chains The hydrophobic strand b4 is located between Cys43 and Asp46 and, as a central strand of sheet 2,...

Ngày tải lên: 29/03/2014, 23:20

... PcsB and StkP [41] The distribution and level of conservation of each of these proteins within pneumococcal strains and the presence in S mitis, S oralis and S infantis are reported in Table and ... Donati et al.: Structure and dynamics of the pangenome of Streptococcus pneumoniae and closely related species Genome Biology 2010 11:R107 Submit your next manuscript to BioMed Central and take full ... genes and the number of core, dispensable (that is, missing in at least one strain), and strain-specific genes for S pneumoniae alone, for S pneumoniae, S mitis, S oralis, and S infantis, and for...

Ngày tải lên: 09/08/2014, 22:23

Ngày tải lên: 14/11/2014, 10:21

... of hCD2 and rat CD2 3D structures indicated that Tyr86 and Glu36 side chains overlap in hCD2 and rat CD2 and oriented in the same direction in both the structures To stabilize the b-turn structure ... Blood Cells (expressing CD58 protein) first and then an equal amount of Jurkat cells (expressing CD2 protein) were added later The cells were pelleted by centrifugation and incubated at °C The cell ... protein CD58 and CD2 expressing on Caco-2 cells and Jurkat cells, respectively, were pre-examined Peptides were added to the confluent Caco-2 monolayer and then the BCECF labeled Jurkat cells were...

Ngày tải lên: 19/02/2014, 13:20

... developments in studying the dynamics of protein structures from 15 N and 13C relaxation time mesurements In Biological Magnetic Resonance: Structure Computation and Dynamics in Protein NMR (Krishna ... (HT1 and HT2) were identified in XPCB–hHR23B, one between helix and helix 3, and one between helix and helix 4–5 These two trails were linked by a hydrophobic linker patch between helix and helix ... presented in yellow, and the polar and charged surfaces are shown in white HT1 and HT2 are hydrophobic trail and 2, respectively HL denotes the hydrophobic linker patch Some structures from the...

Ngày tải lên: 07/03/2014, 17:20

... Leu8, His16, and Gly17, is present in seven structures, and another antiparallel b-bulge (B2), composed of Val57, Glu87, and Arg88, is observed for all the structures The secondary structure elements ... and molecular dynamics (MD) simulation data was carried out Results and Discussion Refined NMR structures of MC1 Chemical shift assignments for MC1 were obtained for 97% of N, HN, Ha, Ca, Cb and ... 1A,B) The secondary structure elements, namely an a-helix, a1 (25– 32), and five b-strands, b1 (4–9), b2 (15–21), b3 (43– 48), b4 (55–65), and b5 (79–90), are all antiparallel and packed with each...

Ngày tải lên: 22/03/2014, 17:20

... compact calmodulin (1PRW.pdb) and NSCP (solution structure) : )111 and )116, respectively Sequence and structural comparison between calmodulin and three different compact EF-hand proteins (NSCP, Botrychium ... proton exchange, correspond to global dynamics, and FEBS Journal 272 (2005) 20222036 ê 2005 FEBS G Rabah et al Structure and dynamics of NSCP in solution Fig Dynamics analysis by amide proton exchange ... from the temperature and co-ordinate rmsd proles, the apo and holo dynamics are of comparable quality Figure 6A compares the two nal simulated structures and the crystal structure, using the...

Ngày tải lên: 23/03/2014, 13:20

... molecular sieves, as structures involved in cell adhesion and surface recognition, as scaffolding for enzymes, as 19 20 Structure of the Bacterial Cell virulence factors and as molecular and ion traps ... Bacterial Cell Cycle: Replication of the Chromosome, Partitioning and Cell Division 51 Replication 51 Replication of Circular Chromosomes 51 Dynamics of the Bacterial Chromosome Structure and Function ... elucidation of the DNA structure by F Crick and J Watson more than 50 years ago and was subsequently expanded by the exploration of the basic mechanisms of DNA replication and gene expression These...

Ngày tải lên: 03/04/2014, 12:05

... Germany) 52 2.2 Cell lines and cell culture HeLa cervical cancer cells and MCF-7 breast cancer cells were purchased and cultured as recommended by the American Type Culture Collection (ATCC) Cells were ... optimal cell plating density for ERα and ERβ cells was determined to be × 104 cells and 0.5 × 104 cells per well, respectively The optimal incubation period was 30 h for ERα or 24 h for ERβ cells ... Optimization of ERα and ERβ cell- based bioassay parameters ERα and ERβ cells were developed by Yap et al., 2005 and optimization of bioassay parameters was performed by seeding cells at various...

Ngày tải lên: 10/09/2015, 15:48

... estradiol and (B) estrone (E1) concentrations via LC-MS/MS; and (C) ERα, (D) ERβ, (E) breast cancer cell (MCF-7) and (F) androgen receptor (AR) bioactivities Data points were the means±SEM and expressed ... steroid 89 3.1.3 Validation of ERα and ERβ cell- based bioassays The inter- and intra-assay variations of both ERα and ERβ bioassays, were determined by using 0.1 nM and 0.5 nM of estradiol, respectively ... -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 [ligand], M 98 3.1.6 Anti-estrogenic activities of SERMs and ER-antagonist in ERα and ERβ cell lines The ability of ERα and ERβ bioassays to detect anti-estrogenic...

Ngày tải lên: 10/09/2015, 15:54

... ERα and ERβ stable cell lines and MCF-7 breast cancer cells to detect estrogenic agonistic and antagonistic activities of mixtures was studied ERα and ERβ stable cells; and MCF-7 breast cancer cells ... Serum samples were obtained and assayed ex-vivo for levels of estrone and estradiol by tandem mass spectrometry, for ERα and ERβ bioactivity and MCF-7 breast cancer cell proliferative effects ... determined to be 8.1% and 16% respectively Detection limits for estradiol in ERα and ERβ bioassays were 8.4 and 13.1 pM, respectively This set of assays is simple and rapid to perform and offers highthroughput,...

Ngày tải lên: 10/09/2015, 15:54

... Serum samples were obtained and assayed ex-vivo for levels of estrone and estradiol by tandem mass spectrometry, for ERα and ERβ bioactivity and MCF-7 breast cancer cell proliferative effects ... ligands, such as icaritin found in Epimedium, which have been reported to be able to initiate the degradation of the ER and suppress estrogen signaling and proliferation of breast cancer cells ... analytical methods coupled with cell- based bioassays that can measure global estrogenicity of sera of animals were employed to understand the levels and bioactivity of conjugated and unconjugated active...

Ngày tải lên: 10/09/2015, 15:54

... 27008–27016 Mak, P., Cruz, F.D and Chen, S 1999 A yeast screen system for aromatase inhibitors and ligands for androgen receptor: yeast cells transformed with aromatase and androgen receptor Environ ... actions and functional roles in health and disease In Nuclear Receptors: Current Concepts and Future Challenges (eds Bunce, C & Campbell, M.J.) 91–141 (Springer, The Netherlands) Nowell, S and Falany, ... enzyme activity and CYP1A1 expression by resveratrol Mol Pharmacol 56(4):760-7 Ciolino, H.P., Daschner, P.J and Yeh, G.C 1999 Dietary flavonols quercetin and kaempferol are ligands of the aryl...

Ngày tải lên: 10/09/2015, 15:54

... different unit cell parameters, may coexist in the same cell (Behnke, 1965, 1968; Landh, 1996) These cells lack a nucleus and the cytoplasmic connection and exchange between vertically stacked cells is ... (Landh, 1994), and in lipid and lipopolysaccharide mixtures (Brandenburg, 1990, 1992) Most cubic phases in lipid–water systems exhibit unit cell parameters not larger than 20 nm, while in cellular ... because of its highly convoluted and dynamic structure and crucial functions in membrane lipid synthesis and assembly, protein synthesis and secretion, ion homeostasis, and membrane quality control...

Ngày tải lên: 11/09/2015, 09:56

... monitoring and audit for policy compliance 13 Firewall implementation and management 14 Virus prevention and protection 15 System and network ownership and management 16 End user accountability and ... Identification and authentication Overview of Security Policies 17 Records retention and backup 18 Security Awareness and education 19 Partner and 3rd party connectivity 20 System development and deployment ... Security Policy - Structure and Guidelines • December 2001 business contracts, trade secrets, and customer privacy; or reduce credibility and reputation with its customers, shareholders and partners...

Ngày tải lên: 17/09/2012, 09:40

... for Denmark, Switzerland, Spain, Finland, France, Germany, Ireland, Italy, the Netherlands, Norway and the UK, a threemonth Treasury bill rate for Belgium, Sweden and the US, and a three-month commercial ... equity and residential property price data: Australia, Belgium, Canada, Denmark, Finland, France, Germany, Ireland, Italy, Japan, the Netherlands, Norway, Spain, Sweden, Switzerland, the UK and ... at the 95% level in Canada, Finland, the Netherlands, Norway, Sweden, Switzerland, the UK and the US Moreover, while in some countries, (including Finland, the UK and the US) residential property...

Ngày tải lên: 24/10/2012, 09:40

... collective self-image and style of the organization; its shared values and beliefs, norms and symbols”5 Each organization has different expectations, values, norms and ways to adapt and identify its ... satisfaction and expert power Finally, person culture takes individual as central focus and talent of individual is target for structure exists As we mentioned about organizational structure and culture ... applied a functional structure This type of structure is effective for managing and easy to focus on product group “This unique structure enables Mozilla to financially support and cultivate competitive,...

Ngày tải lên: 12/04/2013, 21:50