cell organelles epithelial cell

... cells We demonstrate that RPE cells can provide trophic effect on DAergic cells, which may be one of the possible mechanisms underlying RPE cell therapy Previous studies had showed that RPE cells ... whether RPE cells can produce and release DA Our results indicate that 1) RPE cells can express and secrete GDNF and BDNF and protect DAergic cells against neurotxoins-induced injury; 2) RPE cells ... with RPE cells significantly striatum and Microcarriers-RPE cells survive in the hostimprove animal Microcarriers-RPE cells survive in the host striatum and transplantation with RPE cells significantly...

Ngày tải lên: 18/06/2014, 15:20

... apoptotic cell death, the expression of PAR-2, and the activation of MAPK in H pylori-infected gastric epithelial cells, cell viability and DNA fragmentation were determined in the cells transfected ... augments H pyloriinduced cell death and DNA fragmentation in gastric epithelial cells To investigate the relations of PAR-2 expression, cell death, and DNA fragmentation, cells were transfected ... in the cells transfected with PAR-2 AS ODN Viable cell numbers were decreased by H pylori with the number of bacterium infected to the cells (Figure 2A) Cell death of H pylori-infected cells was...

Ngày tải lên: 18/06/2014, 16:20

... contamination with myeloid cells, B cells, T cells or myoepithelial cells (Figure 2d–h) The HSG cell line had exactly the same staining pattern as epithelial cells from patients (positive for cytokeratin ... in epithelial cell lines led us to determine whether they could be extended to primary epithelial cells in patients with pSS and controls Epithelial origin of salivary gland cells To exclude cell ... Figure Immunocytochemical analysis of salivary epithelial cells Cells were obtained from minor salivary glands (a–e) and the HSG cell line (f–i) Positive cells staining for cytokeratin (a) and cytokeratin...

Ngày tải lên: 09/08/2014, 07:20

... between epithelial cells and fibroblasts As the tissue preparation and culture conditions were optimized for epithelial cells, the cell cultures predominantly comprised mammary epithelial cells ... precursor cells [34] but may also appear in cultured epithelial cells [35] To distinguish between different populations of cells, double labeling of cellular cytokeratin and vimentin was performed Cells ... decreased the number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as this amount of cells was not altered...

Ngày tải lên: 10/08/2014, 10:21

... carbohydrate-rich cell wall fraction is capable of inducing specific chemokines and cytokines in cells such as macrophages, dendritic cells (DC) and alveolar epithelial cells [11,12,17,18] Airway epithelial cells ... secretion of IL-8 by airway epithelial cells stimulated with PCBG requires intra-cellular, rather than extra-cellular, calcium mobilization IL-8 secretion by airway epithelial cells is mediated by NFκB ... in epithelial cells responses to PCBG Intracellular calcium mobilization appears necessary for IL-8 secretion, since PCBG does not activate airway epithelial cells in the presence of the intracellular...

Ngày tải lên: 12/08/2014, 11:22

... for the transduction as well as the generation of signals involved in cell- cell as well as in cell- matrix interactions Cell- cell interactions may be direct via contact of tight junction proteins, ... type I epithelial cells (AEC I) [18] As depicted in Figure 3C, essentially all cells stained positive with the lectin, demonstrating high purity of AEC II cells obtained by negative selection cell ... SPC-HA Transfer 93,63% Figure cytokine staining in CD4+ T cells Intracellular Intracellular cytokine staining in CD4+ T cells CD4+ T cells from the lung or bronchial lymph nodes (BLN) from either...

Ngày tải lên: 12/08/2014, 15:20

... an inhibitor of cell injury, on DNA fragmentation, caspase activation, and secretion of HMGB1 from L pneumophila-infected A549 cells Cell culture The human alveolar epithelial cell line A549 was ... 0.05 Results Intracellular growth and cytotoxicity of L pneumophila in A549 cells First, we verified the infection and intracellular growth of L pneumophila in A549 cells Intracellular growth of ... alveolar epithelial cells, suggesting a correlation with the cytopathic effect on the cells This is the first description also of increased HMGB1 protein in the supernatants of alveolar epithelial cells...

Ngày tải lên: 12/08/2014, 15:21

... the expression of cellular genes Methods Cell culture and in vitro HTLV-I infection Human A549, a type II alveolar epithelial cell line, and NCI-H292, a tracheal epithelial cell line, were maintained ... in lung epithelial cells cocultured with HTLV-I infected T cells A549 and NCI-H292 cells were cocultured with either MT2 or CCRF-CEM cells After cocultivation for days, A549 and NCI-H292 cells ... chemokines and cell adhesion molecule in A549 cells cocultured with MT2 cells A549 cells were cocultured with MMC-treated MT-2 or CCRF-CEM cells At and days after cocultivation, A549 cells were harvested...

Ngày tải lên: 13/08/2014, 05:21

... Hodgkin and R-S cells to activated lymphocytes The identification of this antigen in cells as apparently disparate as activated lymphocytes, R-S cells and now human epithelial cells of the developing ... signals for cell proliferation, apoptosis and cytotoxicity in lymphoid cells [20,36,37] Our results give the first indication that the CD30 antigen is expressed in the epithelial cells of developing ... also been reported in other nonlymphoid tissues and cells such as soft tissue tumours [23], decidual cells [24,25], lipoblasts [26], myoepithelial cells [27], reactive and neoplastic vascular lesions...

Ngày tải lên: 13/08/2014, 22:22

... mediates HSV-1 entry into RPE cells Results Attachment of HSV-1 to cell membrane of RPE cells In order to study the initial interaction of HSV-1 virions with cells, live cell imaging was performed ... entry into RPE cells than in CHO-K1 cells, and the absorbance (A) signals representing entry into RPE cells were very similar to those seen with HeLa and Vero cells Both HeLa and Vero cell are naturally ... mediating cell- to -cell fusion [32,33], we also decided to examine the role of nectin-1 in the fusion of RPE cells with viral glycoprotein-expressing cells In a semiquantitative, luciferase-based cell...

Ngày tải lên: 18/02/2014, 14:20

... intestinal mucosal epithelial surface forms the first barrier encountered by the enteric pathogen V cholerae, the intestinal epithelial cell line Int407 and human colonic epithelial cell lines T84 ... Cytokine response in infected epithelial cells A Bandyopadhaya et al Fig Induction of cytokine expression in intestinal epithelial cell lines by V cholerae (A) Int407 and T84 cells were infected with ... 2007 FEBS 4641 Cytokine response in infected epithelial cells A Bandyopadhaya et al 17 Strober W (1998) Interactions between epithelial cells and immune cells in the intestine Ann NY Acad Sci 859,...

Ngày tải lên: 18/02/2014, 16:20

... alveolar epithelial cells of wild-type (+ ⁄ +) and Caveolin-1-knockout (- ⁄ -) lungs Wildtype: Arrow indicates a negative alveolar epithelial type II cell and a neighboring positive type I cell Note ... Analysis of P2X7 receptor expression in alveolar epithelial lung cell lines P2X7 receptor expression was measured in cell lysates of A549, E10, R3 ⁄ and L2 cells by SDS ⁄ PAGE and western blot analysis ... electron microscopy of P2X7 in alveolar epithelial E10 cells Immunogold labeling of P2X7 receptors expressed in E10 cells was performed to analyze the subcellular localization of the receptor in...

Ngày tải lên: 19/02/2014, 00:20

... particulate fraction of epithelial cells Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal cells, Caco-2 cells were investigated ... 272 (2005) 444–453 ª 2004 FEBS Apical iNOS dimer in epithelial cells Experimental procedures Cell culture Human intestinal epithelial DLD-1 cells (ATCC CCL-221) were cultured and stimulated with ... in human bronchial epithelial cells FEBS Journal 272 (2005) 444–453 ª 2004 FEBS Apical iNOS dimer in epithelial cells induces c-fos and stimulates the cGMP pathway Am J Respir Cell Mol Biol 11,...

Ngày tải lên: 19/02/2014, 16:20

... of eRBCC appears to be present in the nucleus of the epithelial cells of the secondary lamella including the chloride cells and pavement cells whose nuclei are labeled by arrows and double arrowheads, ... demonstrated that the chloride cells can be classified into two types based on the locations in the gill: filament chloride cells and lamellar chloride cells The lamellar chloride cells are considered to ... type of cells expressing eRBCC Serial sections were stained with anti-eRBCC and anti-(Na+,K+-ATPase) In freshwater specimens, anti-eRBCC immunostaining was observed mainly in epithelial cells of...

Ngày tải lên: 17/03/2014, 10:20

... carcinoma cell line Caco-2 was obtained from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) The renal cell line SKPT-0193 Cl.2, established from isolated cells ... studies, cells were cultured on permeable polycarbonate TranswellÒ cell culture inserts (24 mm diameter, lm pore size; Corning Costar Bodenheim GmbH, Germany) with a starting cell density of 44 000 cellsÆcm)2 ... apotransferrin (5 lgÆmL)1) Cells were subcultured in Petri dishes with a starting cell density of 83 000 cellsÆcm)2 or in TranswellÒ chambers with a seeding density of 133 000 cellsÆcm)2 Uptake and...

Ngày tải lên: 23/03/2014, 12:20

... in human RPE cells [23], human renal epithelial cells [56], and A549 human lung cancer cells [57] Thus, TGF-b1 is a candidate that may mediate the hypoxic induction of HO-1 in RPE cells In fact, ... ARPE-19 cells The differential effects of TNF-a and IL-1b in the two RPE cell lines may reflect the heterogeneity of RPE cells [31,48] Effects of hypoxia on the expression of HO-1 in human RPE cell ... is reduced in human cell lines by treatment with interferon-c [26,27] or by hypoxia in primary cultures of human umbilical vein endothelial cells, coronary artery endothelial cells, and astrocytes...

Ngày tải lên: 23/03/2014, 13:20

... HAT is specifically found in ciliated epithelial cells; however, it is not found in the basal and goblet cells in the epithelium or in the submucosal gland cells [4] Therefore, it is thought that ... 17 intracellular free Ca2+ concentration in human bronchial epithelial cells J Med Invest 50, 95–107 Schmidlin F & Bunnett NW (2001) Protease-activated receptors: how proteases signal to cells ... phosphorylation of EGFR mediated by AR in NCIH292 cells NCI-H292 cells were stimulated with HAT (200 nM) or AR (3 ngÆmL)1) for the indicated durations (A, B) NCI-H292 cells were pretreated with the vehicle...

Ngày tải lên: 30/03/2014, 11:20

... ependymal cells (Table 1) Interestingly, Kolmer cells (macrophage-like cells) but not the epithelial cells were found to express high levels of HLAclass II (Table 1) Tumour-derived ependymal cells ... na Abbreviations used: CP, choroid plexus epithelial cells; Ep, ependymal cell layer; M, male; F, female; K, Kolmer cells; 0: negative; +: very few cells positive; ++: positive; +++: strongly ... brain epithelial cells and potentially driving robust expression of regulatory proteins to protect from bystander complement attack In human meningitis, we found that epithelial cells and glial cells...

Ngày tải lên: 19/06/2014, 22:20

... diploid epithelial cell line from normal adult rat liver with phenotypic properties of 'oval' cells Experimental Cell Research 1984, 154:38-52 44 Mosmann T: Rapid colorimetric assay for cellular ... tumor cell lines The Journal of Cell Biology 1992, 118:1213-1221 30 Garber SA, Fernstrom MJ, Stoner GD, Ruch RJ: Altered gap junctional intercellular communication in neoplastic rat esophageal epithelial ... Schlemmer SR, Kaufman DG: Endometrial stromal cells regulate gapjunction function in normal human endometrial epithelial cells but not in endometrial carcinoma cells Molecular Carcinogenesis 2000, 28:70-75...

Ngày tải lên: 20/06/2014, 00:20

... ultrasonic bath for 30 minutes And stock solution was stored at −20oC until use Cell culture Rat lung epithelial cell, rat type II epithelial origin, was obtained from ATCC (Manassas, USA) and cultured ... PMs, rat lung epithelial cells were treated with different concentrations of PMs for day, and Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells 13 TTGTTTCTCGT-3', ... rat lung epithelial cell, but also to provide the biological data for the risk assessment of PMs In MTT assay, PM2.5 has shown much highly cytotoxic than PM10 in rat lung epithelial cells and...

Ngày tải lên: 07/08/2014, 17:22