cell lines and growth condition
Genetic approaches to study liver development in zebrafish core component sec13 in COPII complex is essential for liver development in zebrafish
Ngày tải lên :
14/09/2015, 12:11
... kinds of cells in the body, the germ cells, which are set aside and exclusively responsible for reproduction, and the remaining somatic cells The germ cells include gametes (sperms and eggs) and their ... 49 2.11 Cell culture 50 iv 2.11.1 Cell lines and growth condition 50 2.11.2 Subculturing cells 50 2.11.3 Cell transfection 51 2.12 Immunofluorescence ... 1.6 Rational and aim of the project 31 Chapter General Materials and Methods 39 2.1 Fish lines and maintenance conditions 39 2.2 Chemical solutions and growth medium...
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Thiết lập quy trình điện di protein SDS -PAGE và ứng dụng đánh giá phản ứng của cây lúa
Ngày tải lên :
06/11/2012, 09:49
... Saravis C A and Maurer H R., 1984 Gel electrophoresis and Isoelectrics Focusing of protein: Selected Techniques De Gruyter, Berlin Davis B J., 1964 Disc electrophoresis II Method and appication ... Jay A Glasel and Murray P Deutscher, 1995 Introduction to Biophysical Methods for Protein and Nucleic Acid Research Electrophoretic Method pp 53 – 109 37 11 Department of health and ageing office ... and ageing office of the gene technology regulator, 2005 The biology and ecology of rice (O sativa) in Australia 12 Hurkman and Tanaka, 1986 “Phenol extraction followed by methanolic ammonium...
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MÔ HìNH HOá QUá TRìNH TRAO ĐổI NHIệT ẩM TRONG MáY ấP TRứNG GIA CầM Modeling of heat and mass transfer in chicken egg incubators
Ngày tải lên :
28/08/2013, 08:31
... (1980), Heat and mass transfer, Mir Publisher, Moscow Peebles E D, Christopher D McDaniel (2004), A Practical Manual for Understanding the Shell Structure of Broiler Hatching Eggs and Measurement ... Mathematical Simulation of Mass and Heat Transfer in High Moisture Foods, Trans ASAE, pp 55-59 224 Husain A., Chen C.S., Clayton J T (1973), Simultaneuos Heat and Mass Diffusion in Biological ... Solar drying systems: Simulations and optimization, Agrotech Publishing Academy, Udaipur, pp 131-133 Haghighi K., Segerlind L.J, (1988), Modeling Simultaneous Heat and Mass Transfer in Isotropic...
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Effect of periodic suction on three dimensional flow and heat transfer past a vertical porous plate embedded in a porous medium
Ngày tải lên :
05/09/2013, 14:58
... boundary conditions are u11 = 0, θ 11 = at y = 0, u11=0, θ 11 = as y→ ∞ (45) (43) Solving equations (43) and (44) under boundary condition (45) and using equations (18), (22), (25) and (26), ... the velocity and temperature of the flow field are solved employing perturbation technique and the effects of the flow parameters on the velocity and temperature of the flow field and also on ... of the porous medium, ν is the coefficient of kinematic viscosity and k is the thermal conductivity The initial and the boundary conditions of the problem are u* = 0, v* = -V (1+εcosπz* / d), w*...
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Arsenic and membrane toxicity
Ngày tải lên :
17/12/2013, 12:23
... động vào trình đồng hóa glucose Các vò trí đánh dấu Toxicology and Applied Pharmacology 197 (2004) 67– 83 F.S Walton et al / Toxicology and Applied Pharmacology 198 (2004) 424–433 Cơ chế dẫn truyền ... et al./Toxicology and Applied Pharmacology 198 (2004) 424– 433 Vai trò protein có chứa nhóm lưu hình (-SH) Cơ chế gây chết bên tế bào DMAsV vai trò Glutathion (GSH) (Toxicology and Applied Pharmacology ... +5 đến +3 đến +5 E1 MMA(V) reductase E2 arsenic methyltransferase E3 MMA(V) reductase Toxicology and Applied Pharmacology 198 (2004) 327–335 từ là • Arsenic – Nhiều quốc gia bò ảnh hưởng – Các...
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Tài liệu UNIVERSITY ENTREPRENEURSHIP AND TECHNOLOGY TRANSFER: PROCESS, DESIGN, AND INTELLECTUAL PROPERTY doc
Ngày tải lên :
19/02/2014, 05:20
... PhDs in science and engineering, management and economics, and MBA and law students Five factors are included in PhD training – managing R&D for business growth, balancing long-term and short-term ... AND ENTREPRENEURSHIP EDUCATION Arthur A Boni and S Thomas Emerson 241 ORGANIZATIONAL MODULARITY AND INTRA-UNIVERSITY RELATIONSHIPS BETWEEN ENTREPRENEURSHIP EDUCATION AND TECHNOLOGY TRANSFER Andrew ... science and technology research system and with faculty, students, and administrators An interdisciplinary entrepreneurship program provides an excellent environment for commercializing research and...
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Tài liệu Taxation and Technology Transfer: Key Issues doc
Ngày tải lên :
19/02/2014, 08:20
... of poor harvests and demanded that landlords pass the benefit on to their tenants Because the empire depended heavily on the land tax and landowners resisted strongly, this demand was not met Because ... landscape In the coastal provinces, conditions for the rapid growth of TVEs were favorable, but this was far less the case in the central and western parts of the country where the rate of growth ... Ming and the Qing, there was a long-standing commitment never to raise the land tax ( yong bu jia fu) In 1713, the Kangxi emperor froze the land tax at the 1711 level, regardless of changes in land...
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Tài liệu Taxation and Technology Transfer: Key Issues ppt
Ngày tải lên :
19/02/2014, 08:20
... own head office and with others The PE is treated as if it had bought and sold goods from and to its head office and had borrowed money and paid interest, rent, management fees and the like, in ... researchers and policy makers on taxation and technology export and import UNCTAD's work in this area is ongoing, and comments on this preliminary study are welcome Technology Transfer and Taxation: ... Taxation: Key Issues and Introduction Technology is considered an essential precondition for improving productivity, attaining industrial development and promoting export growth Therefore, technology...
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Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
Ngày tải lên :
19/02/2014, 16:20
... applicator 8/1 (code no 18-1816-01) The running condition and staining method followed the procedures given in PhastSystem handbook (ref no 80-1312-29 and 80-1312-30, respectively) Compositions of ... which also displays the results for IFP–F13 and IFP–V13 All peptide analogues exhibit diffuse bands and migrate roughly as dimeric species, with F13 and V13 variants forming oliogomers of slightly ... using a micelle as a model Figure 7A summarizes the NOE interactions from proton pairs with ˚ distance ¼ A in SDS micellar dispersion and in aqueous buffer solution at pH 5.0 The daN(i,i+3) and daN(i,i+4)...
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Tài liệu Báo cáo khoa học: "Syntactic and Semantic Transfer with F-Structures*" pptx
Ngày tải lên :
20/02/2014, 18:20
... input to and output of a transfer component developed within the Verbmobil project (Dorna and Emele, 1996a) The term representation is inspired by earlier work (Kay et al., 1994; Caspari and Schmid, ... representation for transfer and generation The translation between f-structures and terms is based on the correspondence between directed graphs representing f-structures and the functional interpretation ... gerne and the English verb like (see (lb) and (3b)) (la) is the LFG f-structure for the German sentence (lb) (lc) is the set of terms representing (la) Transfer works on source language (SL) and...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
Ngày tải lên :
21/02/2014, 01:21
... known GSTs classes and S mansoni GSTs, allowed us to place the new parasite product among the newly identified GSTO class, and the previously characterized Sm28GST and Sm26GST as mu- and sigma-class, ... hydroquinone and p-chloranil In contrast, SmGSTO showed DHA and HEDS-measured TTase activities Kinetic parameters were obtained for DHA and TTase activities SmGSTO showed a Km ¼ 0.23 mM for HEDS and a ... oxide synthase and nitric oxide production by macrophages J Immunol 160, 3471– 3479 Allaoui, A., Francois, C., Zemzoumi, K., Guilvard, E & Ouaissi, A (1999) Intracellular growth and metacyclogenesis...
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Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt
Ngày tải lên :
07/03/2014, 05:20
... envelope between 11 and 26 charges (m ⁄ z 4982–2108) was observed, and for hexamers and nonamers, complete envelopes between 20 and 35 charges (m ⁄ z 5480–3132) and between 28 and 37 charges (m ... 1.2 and 1.9 kV, and cone and RF lens potentials were 100 and 38 V, respectively The pumping of the ESI interface region was restricted; backing pirani vacuum gauge from 1.8 to 1.95 mbar, and ... donor and acceptor, FRET experiments indicate molecular proximity of Tyr residues in CTC and the bound bis-ANS percentages of a-helix and b-sheet structures decreased, whereas that of random...
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Báo cáo Y học: Properties of the Na+/H+ exchanger protein Detergent-resistant aggregation and membrane microdistribution potx
Ngày tải lên :
08/03/2014, 16:20
... into mL of MBS (25 mM Mes, pH 6.5, 0.15 M NaCl and 1% Triton X-100) Cells were solubilized at °C for 30 and scraped from the dishes and collected Cells were homogenized with a Dounce homogenizer ... proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation Mol Cell 6, 1425–1436 25 Fliegel, L (1999) Functional and cellular regulation of the myocardial Na+/H+ ... NaCl/Pi pH 7.4 for 15 Cells were then washed twice with 0.1% Triton-X 100 and 0.1% BSA in NaCl/Pi (TA-NaCl/Pi) and then permeabilized by incubation in the same solution for 30 The cells were then washed...
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Báo cáo " Sol-gel synthesis and particle size characterization of CdSe Quantum dots " ppt
Ngày tải lên :
14/03/2014, 13:20
... long time reaction and its distribution in particle size is in the broadening range In this article, we report on the preparation of CdSe quantum dots (QDs) by sol-gel method and investigate their ... spectrophotometer Result and discussion To calculate the particle size of QDs we use some following models: + Using absorption spectra to estimate the mean sizes of QDs: The Effros, Brus and Kayanuma’s ... between mean size and specific parameters of QDs [7]: a a E g (a ) = E g + π R B − 1,786R * B − 0,248R * y y a a * y (1) where E g(a) is the effective band gap of QDs with...
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Báo cáo khoa học: Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain" doc
Ngày tải lên :
14/03/2014, 22:20
... residues Trp50 and Trp153 are located in the C domain, in b-strand CB3 and just after strand CB7, respectively, according to the crystal structure of diphtheria toxin [16–18] (Fig 1) Trp206 and Trp281 ... the C and T domains influences the membrane interaction and the associated conformational changes of the other domain We compared the pH sensitivities and the membrane interactions of the C and ... the CTs, the C and T domains were folded at basic pH and adopted their known MG state at acidic pH We studied, by CD spectropolarimetry in the far-UV and near-UV, the secondary and tertiary structures...
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Chapter 26 Remote Logging, Electronic Mail, and File Transfer pot
Ngày tải lên :
15/03/2014, 12:20
... Content-transfer-encoding 26.33 Figure 26.16 SMTP range 26.34 Figure 26.17 Commands and responses 26.35 Figure 26.18 Command format 26.36 Table 26.7 Commands 26.37 Table 26.8 Responses 26.38 Table 26.8 Responses ... to send an e-mail and simulate the commands and responses we described in this section We use TELNET to log into port 25 (the well-known port for SMTP) We then use the commands directly to send ... of items in a directory The colored lines show the responses from the server control connection; the black lines show the commands sent by the client The lines in white with a black background...
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Cadmium and zinc interactions and their transfer in
Ngày tải lên :
15/03/2014, 23:22
... WA was 47 and 17 times higher than the contents found at YA and MA in spring wheat lands, respectively, and 46 and 20 times in corn lands, respectively Soil Zn concentration at WA was and 1.5 times ... applications and industrial wastewater irrigation, and partially from aerial deposition and the usage of fertilizers and pesticides ŽMench et al., 1994; Ullrich et al., 1999; Nan and Zhao, 2000; ... at WA was and 1.5 times higher than the contents found at YA and MA in spring wheat lands, respectively, and and 2.4 times in corn lands, respectively These facts could be the result of differences...
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