cell free selection of domain antibodies by in vitro compartmentalization
Báo cáo sinh học: "Regulatable systemic production of monoclonal antibodies by in vivo muscle electroporation" docx
... resulting from repeated injections of massive doses of antibodies and (iii) the restrictive costs of in vitro produced proteins certified for human use Moreover, injection of massive doses of mAbs ... therapy 2004 in press Noel D, Pelegrin M, Marin M, Biard PM, Ourlin JC, Mani JC, Piechaczyk M: In vitro and in vivo secretion of cloned antibodies by genetically modified myogenic cells Hum Gene ... long-term delivery of antibodies in immunocompetent animals using cellulose sulphate capsules containing antibody-producing cells Gene Therapy 1998, 5:828-834 Pelegrin M, Marin M, Oates A, Noel...
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Báo cáo khoa học: Engineering thermal stability of L-asparaginase by in vitro directed evolution ppt
... The rates of inactivation were followed by periodically removing samples for assay of enzymatic activity Observed rates of inactivation (kin) Engineering thermal stability of L-asparaginase were ... state, improvement of molecular packing (e.g shortening of loops, improvement of interactions in the hydrophobic core, for example, by the removal of internal cavities), modification of surface charge ... Enhancing the stability of l-ASNase by protein engineering improves its body-residence time, and thereby minimizes immunosuppressive effects by lowering the therapeutic dose After one round of directed...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in ... that of the original model (Fig 3E) Interestingly, the inclusion of IKK2 inhibition by SC-514 with our experimentally determined rate constants in the original model resulted in a delay in simulated ... for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based and in silico analysis)...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
... 15N-labelled by transamination In this situation, the main effect of transamination is a reduction of the degree of 15N-labelling in the amino acid of interest In the case of [15N]Gly, much of the label ... depleted in natural amino acids Absence of free amino acids from the cell extract is a prerequisite for efficient incorporation of labelled amino acids into target proteins Furthermore, the protein ... production of many different proteins Ó FEBS 2004 Cell- free synthesis of 15 N-labelled proteins (Eur J Biochem 271) 4087 Optimization of other conditions for in vitro protein synthesis Fig Cell- free...
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Studies on the antibody repertoire in a dengue virus immune subject and isolation of neutralizing antibodies by phage display technology
... and incubated at 37°C, 5% CO2 Upon reaching confluence, the splitting of cell 34 monolayer was done by incubating the cells with Trypsin-EDTA (Gibco, Invitrogen) at 37°C for 2-3 minutes Cells ... binding of the Fc portion of antibody to induce ADE was demonstrated by Balsitis et al, whereby the use of Fab and non- Fcϒ-receptor binding variant (N297Q) resulted in the neutralization of infection ... for the in vitro selection of monoclonal antibodies against a given antigen Principally, a protein-encoding (in this case an antibody) is fused to a bacteriophage coat protein resulting in phage...
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KếT QUả BƯớC ĐầU TUYểN CHọN GIốNG XOàI ĐịA PHƯƠNG TạI HUYệN YÊN CHÂU, TỉNH SƠN LA Initial Results on Clone Selection of Local Mangoes Grown in
... 25 T ht Hng nm 2-3 6-7 3.2 Một số đặc điểm sinh trởng v hình thái xoi tuyển chọn Một số đặc điểm thực vật học xoi đợc tuyển chọn trình by bảng - Về sinh trởng cây: Do nhân giống từ hạt nên tất ... khác nhiều, nằm khoảng 3,5 - 4,3 cm Bảng Một số đặc điểm sinh trởng v hình thái xoi tuyển chọn Dng tỏn Cao cõy (m) Di phin lỏ (cm) Rng phin lỏ (cm) Di cung lỏ (cm) 7,1 Hỡnh chúp cao 8,9 24,5 4,63 ... điều tra thiết kế sẵn để xác định cá thể có đặc tính nông sinh học vợt trội, sau tiến hnh theo dõi trực tiếp tiêu đặc điểm hình thái, sinh trởng, hoa, đậu quả, suất, chất lợng cá thể năm liên tục...
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Báo cáo sinh học: " Selection of Recombinant MVA by Rescue of the Essential D4R Gene" doc
... visualized by crystal violet staining Western blotting Whole cell lysates were prepared from × 106 infected cells by three cycles of freezing/thawing, followed by ultrasonication and denaturation in ... characteristic of the cell line of choice To evaluate the cell line DF-1 for the intended selection of recombinant replicating MVA, we assessed it for propagation of MVA using primary CEF as a reference In ... for rapid isolation of the target recombinant, resulting in parental virus -free recombinant stocks in a single round of plaque purification While generation of recombinants of the highly attenuated...
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Báo cáo hóa học: " Selection of Recombinant MVA by Rescue of the Essential D4R Gene" docx
... visualized by crystal violet staining Western blotting Whole cell lysates were prepared from × 106 infected cells by three cycles of freezing/thawing, followed by ultrasonication and denaturation in ... characteristic of the cell line of choice To evaluate the cell line DF-1 for the intended selection of recombinant replicating MVA, we assessed it for propagation of MVA using primary CEF as a reference In ... for rapid isolation of the target recombinant, resulting in parental virus -free recombinant stocks in a single round of plaque purification While generation of recombinants of the highly attenuated...
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Báo cáo hóa học: " Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses" pot
... Pfeiffer T, Starck L, Steinhauer DA: Inhibition of release of lentivirus particles with incorporated human influenza virus haemagglutinin by binding to sialic acid-containing cellular receptors J ... proteins and a recombinant genome encoding the green fluorescent protein (GFP) [16,19] Transduction of this marker gene in 'infected' target cells and expression of GFP in transduced cells is indeed ... effective release of VLPs into the cell supernatant in the absence of NA Indeed treatment of these latter cells with purified neuraminidase from Vibrio cholerae induced efficient release of the viral...
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báo cáo hóa học:" Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model" potx
... rodent spine in flexion-extension testing Incorporating the same methodology used in human spine testing, we were able to assess spinal fusion in the rodent model In humans, quality of spinal fusions ... osteoinduction by radiographic examination Microcomputed Tomography inspection of explanted spines exposed to BMP-2 was performed taking an average hours/spine (including preparation, scanning, ... spinal arthrodesis [10] Often heterotopic bone bridging transverse processes of the vertebrae was not capable of restricting intervertebral motion during spinal flexion/extension In our testing,...
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báo cáo hóa học:" Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model" pptx
... rodent spine in flexion-extension testing Incorporating the same methodology used in human spine testing, we were able to assess spinal fusion in the rodent model In humans, quality of spinal fusions ... osteoinduction by radiographic examination Microcomputed Tomography inspection of explanted spines exposed to BMP-2 was performed taking an average hours/spine (including preparation, scanning, ... spinal arthrodesis [10] Often heterotopic bone bridging transverse processes of the vertebrae was not capable of restricting intervertebral motion during spinal flexion/extension In our testing,...
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báo cáo hóa học:"Decreased health-related quality of life in disease-free survivors of differentiated thyroid cancer in Korea" potx
... disease -free survivors of DTC and the control group are shown in Table Disease -free survivors of DTC had greater levels of distress according to the HADS-A and BFI scores Interestingly, the disease -free ... carcinoma 308 97.5 Determinants for HRQOL Follicular carcinoma 2.5 Anxiety, depression and fatigue emerged as the strongest determinants for most of the domains in the EORTC QLQ-C30 in disease -free ... QLQ-C30 of the disease -free survivors of DTC and the control group are compared in Figure The disease -free survivors of DTC showed significantly lower scores in all of the functional domains (physical,...
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báo cáo khoa học: "Experimental comparison of methods for simultaneous selection of two correlated traits in Tribolium. 2. Index selection and independent culling levels : a replicated single generation test" potx
... index line exceeded the independent culling line, being about twice as good In all replicates selection response was greater in the I line than in the N line The analysis of variance of the selection ... weight and egg laying, respectively The selection intensities for egg laying and adult weight in both lines are shown in table In the independent culling line the intensity of selection was larger ... culling method, in the present experiment selection was practised for egg laying and adult weight of Tribolium, comparing index selection and independent culling levels in ten replicates of a single...
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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" pdf
... were defined within single cells, and the average fluorescence intensity of each region of interest was measured The role of WIN62577 on ASMC proliferation induced by IL-13 was next investigated ... chemotropism [27] In this experiment, we demonstrated that NK-1R antagonist WIN62577 had the effect of inhibiting ASMC migration in vitro, indicating that WIN62577 may contribute to the inhibition of airway ... concentration in ASMC, while WIN62577 caused it to decline Serca2 and Ip3r mRNA expression in different groups The equilibrium of Ca2+ content in the SR is maintained by SERCA pumping calcium in, while...
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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" potx
... were defined within single cells, and the average fluorescence intensity of each region of interest was measured The role of WIN62577 on ASMC proliferation induced by IL-13 was next investigated ... chemotropism [27] In this experiment, we demonstrated that NK-1R antagonist WIN62577 had the effect of inhibiting ASMC migration in vitro, indicating that WIN62577 may contribute to the inhibition of airway ... concentration in ASMC, while WIN62577 caused it to decline Serca2 and Ip3r mRNA expression in different groups The equilibrium of Ca2+ content in the SR is maintained by SERCA pumping calcium in, while...
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báo cáo khoa học:" Decreased health-related quality of life in disease-free survivors of differentiated thyroid cancer in Korea" pptx
... disease -free survivors of DTC and the control group are shown in Table Disease -free survivors of DTC had greater levels of distress according to the HADS-A and BFI scores Interestingly, the disease -free ... carcinoma 308 97.5 Determinants for HRQOL Follicular carcinoma 2.5 Anxiety, depression and fatigue emerged as the strongest determinants for most of the domains in the EORTC QLQ-C30 in disease -free ... QLQ-C30 of the disease -free survivors of DTC and the control group are compared in Figure The disease -free survivors of DTC showed significantly lower scores in all of the functional domains (physical,...
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Báo cáo sinh học: "Localization of leucocyte interferon gene in the q2.5 region of pig chromosomel by in situ hybridization" potx
... by in situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon ... combined with the use of high-resolution chromosome spreads, will be useful in several fields: localization of unique sequences, increasing the precision of gene localization on chromosomes (Lin ... method thereby, permitting more reproducible results The high specific activity could partly explain the efficiency of in situ hybridization This observation confirms the one given by Lin et al...
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Study of domain wall devices in magnetic nanowires
... of domain walls in nanowires The most common approach to pin a domain wall is by introducing a constriction along the lateral edge of the nanowire The parameters such as pinning fields and pinning ... cross-tie domain wall (XDW) A XDW consists of a main DW, separating two antiparallel magnetic domains The structure of the main wall varies continuously along its length, comprising alternating Néel ... for imaging the domain structure in magnetic materials Even though the types of domain wall can be distinguished by AMR measurements, it cannot be correlated without imaging the domain structure...
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Comparative study of HLA b27 peptide binding specificity by in vitro refolding assay
... of the pockets and therefore 11 Introduction Peptide A α2 Domain β2-Microglobulin α3 Domain B α1 Domain α1 Domain α2 Domain Figure 1.4 Ribbon diagram showing the three-dimensional structure of ... Schematic diagram of six pockets defined in the peptide-binding cleft of HLA class I molecules (top view of peptide-binding cleft) A, residues of HLA class I molecules identified by their amino acid position ... and B*2717 differ from B*2705 by a single amino acid substitution: tyrosine at position 59 is substituted by histidine in case of B*2703, and by phenylalanine in case of B*2717 B*2704, which seems...
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