cell culture organ systems imaging approaches in blood brain barrier research
Báo cáo y học: " In vivo imaging approaches in animal models of rheumatoid arthritis" pot
Ngày tải lên :
09/08/2014, 01:22
... possible An alternative mode of imaging is the use of single photon imaging of γ-emitting isotopes like 11 1In or 99Tc Imaging of γ-emitting isotopes is referred to as single photon emission commuted ... transferred lymphocytes A promising technology for tracking cells deep in animals is the use of paramagnetic contrast agents taken into cells using cell- penetrating peptides in conjunction with MRI [15,16] ... rheumatologists interested in surgical procedures for in vivo imaging, there is a rich arsenal of procedures for imaging within almost all major organs of mice or rats Almost all were developed originally...
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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf
Ngày tải lên :
19/06/2014, 22:20
... Results Anandamide inhibits VCAM-1 induced by TMEV in brain endothelial cells by CB1 receptors The endothelial blood brain barrier protects the CNS from the changing environment in both physiologic ... an in vitro model of the blood- brain barrier, comprising a co -culture of brain capillary endothelial cells and astrocytes Eur J Pharm Sci 2001, 12:215-222 31 Eugenin EA, Berman JW: Chemokine-dependent ... when cleaved by intracellular sterases Cell cultures b.End5: Murine brain endothelial cells (b.End5) which are recognized to present brain endothelium like properties were obtained from European...
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Báo cáo y học: "Effect of magnesium sulfate administration on blood–brain barrier in a rat model of intraperitoneal sepsis: a randomized controlled experimental study" ppt
Ngày tải lên :
12/08/2014, 20:20
... mechanism of brain injury in sepsis Studies have suggested that septic encephalopathy might involve a disturbance of plasma and brain neutral amino acid transport across the blood brain barrier (BBB), ... compared within groups using a Wilcoxon signed rank test Two-tailed P values are shown in the last column Table Assessment of blood brain barrier permeability by Evans blue dye content in brain tissue ... obtained in our sepsis model; however, the changes in SG were higher in the traumatic brain injury model than in our sepsis model This R21 might be explained by the different mechanisms causing...
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Báo cáo y học: "Inhibition of complement C5a prevents breakdown of the blood-brain barrier and pituitary dysfunction in experimental sepsis" ppt
Ngày tải lên :
13/08/2014, 15:21
... with surrounding brain cells, relaying into the brain inflammatory response and jeopardising the functional integrity of the BBB [55-57] In the present study, we found that, during experimental ... tightly regulating the passage of molecules into and out of the brain to protect the brain from microorganisms and neurotoxic substances During sepsis, however, blood- borne proinflammatory mediators ... Annane D: Brain lesions in septic shock: a magnetic resonance imaging study Intensive Care Med 2007, 33:798-806 57 Sharshar T, Hopkinson NS, Orlikowski D, Annane D: Science review: The brain in sepsis...
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Application of biologically active micelles in drug delivery across the blood brain barrier
Ngày tải lên :
11/09/2015, 09:10
... Major cell types in the brain 46 6.1.1 Neurons 46 6.1.2 Main non-neuronal cells 47 6.1.3 NG2 positive cells 49 6.2 Pathological changes of brain cells in brain ... system (CNS) is to overcome the barrier preventing the drugs moving into the brain parenchyma The blood brain barrier (BBB) lies between the blood flow and the brain parenchyma The BBB prohibits ... entering from the blood to brain, including CNS drugs The limited penetration of drugs through the BBB into the brain parenchyma is the rule, not the exception In the treatment of brain infection...
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the blood – brain barrier
Ngày tải lên :
11/04/2014, 10:34
... in blood- brain barrier permeability to protein Acta Neuropathol (Berl) 90, 454–460 Nag S (1998) Blood- brain barrier permeability measured with histochemistry In Introduction to the Blood- Brain ... non-neural blood vessels as well (108) Cadherins are linked intracellularly to a group of proteins termed catenins (109) α-catenin is a vinculin homolog that binds to β-catenin and probably links cadherins ... transpeptidase in isolated brain endothelial cells: induction by glial cells in vitro Science 207, 635–645 Janzer, R C., and Raff, M C (1987) Astrocytes induce blood- brain properties in endothelial cells...
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báo cáo hóa học: " Lipopolysaccharide-enhanced transcellular transport of HIV-1 across the blood-brain barrier is mediated by luminal microvessel IL-6 and GM-CSF" potx
Ngày tải lên :
19/06/2014, 22:20
... Permeability of blood- brain barrier to various sized molecules Am J Physiology 1985, 248:H712-H718 Chou S, Dix RD: Viral infections and the blood- brain barrier In Implications of the Blood- Brain Barrier ... enhanced invasion of HIV-1 into the brain may be mediated by BMEC-derived cytokines secreted into blood or by blood- borne cytokines Consistent with this, IL-6 in the blood compartment induces ... BBB: Blood- brain barrier; BEC: Brain endothelial cells; BMEC: Brain microvascular endothelial cells; cART: Combination antiretroviral therapy; GM-CSF: Granulocyte-macrophage colony-stimulating...
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báo cáo hóa học: " Neuro-Inflammation, Blood-Brain Barrier, Seizures and Autism" pot
Ngày tải lên :
19/06/2014, 22:20
... as well as in inflammation [26] Substances originating in the gut or the brain can trigger mast cells to release mediators that could disrupt the gutblood barrier and blood- brain barrier (BBB), ... Corticotropin-releasing hormone (CRH) and brain mast cells regulate blood- brain- barrier permeability induced by acute stress J Pharmacol Exp Ther 2002, 303:1061-1066 51 Theoharides, T C and Konstantinidou, ... behavior in mice [70] Luteolin also inhibits mast cell- dependent stimulation of activated T cells [71], as well as activated peripheral blood mononuclear cells from patients with the brain inflammatory...
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Hình thái và chức năng của hàng rào máu não (blood brain barrier)
Ngày tải lên :
19/07/2014, 20:48
... tế bào chất gắn với ß-catenin plakoglobin, đến chu kỳ lại gắn α-catenin, α-actinin vinculin, mục đích làm ổn định phức hợp vùng dính Dải bịt Lòng mạch Phức hợp protein Khoảng gian bào Màng tế ... trúc phân tử Occludin[12] 22 4.2.3.Claudin Claudin có vị trí màng tế bào giống occludin đồng đẳng occludin Claudin protein có trọng lượng phân tử 20-24 kDa Có 24 đồng đẳng claudin phát động vật ... cầu CD11b/CD18 integrins ICAM-1, iC3b, Bạch cầu đơn nhân, đa fibrinogen nhân , lympho iC3b Bạch cầu đơn nhân, (Mac-1) CD11c/CD18 integrins đa nhân VLA-4 integrins VCAM-1 fibronectin Tất cả, trừ...
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Nanoparticles of biodegradable polymers for delivery of therapeutic agents and diagnostic sensitizers to cross the blood brain barrier (BBB) for chemotherapy and MRI of the brain
Ngày tải lên :
26/11/2015, 22:48
... ONE: INTRODUCTION 1.1 BLOOD BRAIN BARRIER 1.1.1 History and Anatomy of the Blood Brain Barrier 1.1.2 Functions of the Blood Brain Barrier 1.1.3 Clinical Significance of the Blood Brain Barrier ... Disturbing the blood brain barrier includes osmotic blood brain barrier disruption [40-44] and biochemical blood brain barrier disruption [45-47] Alternative routes to CNS drug delivery include intraventricular/intrathecal ... dosing and (Right) controlled delivery dosing 19 2.2 BRAIN CANCER AND OTHER BRAIN DISEASES A brain tumor is a mass of unnecessary cells growing in the brain [94] There are two basic kinds of brain...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
Ngày tải lên :
18/02/2014, 08:20
... capable of mimicking or evoking the potentiation effect induced by JHIII in this assay Experimental procedures Cell culture, EMSA, and western immunoblotting All aspects of cell culture methodology, ... sites inserted by the PCR primers are underlined The resulting LdUSP amplicon includes the entire DBD, whereas LdUSP ⁄ DDBD includes the entire ORF beginning at the first amino acid following the ... candidate insecticidal compounds showing both inductive and potentiative activity Results The DNA-binding domains (DBDs) of Leptinotarsa and Drosophila EcR and USP are identical at every amino acid...
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Báo cáo khoa học: The influence of cold shock proteins on transcription and translation studied in cell-free model systems pdf
Ngày tải lên :
07/03/2014, 21:20
... enzymes cleaving the target protein Other effects which can occur in whole cells (e.g the induction of new proteins interfering with the protein expression) cannot occur in our isolated in vitro ... existing in vivo, the unspecic inhibition of protein expression observed in the living cell after a cold shock can be explained by a direct binding of CSP to RNA and or DNA Besides this, in our ... as an activating factor after binding to specic mRNAs [20] The starting point for our experiments was the idea to use CSPs from different organisms in cell free expression systems in order to...
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basic cell culture protocols methods in molecular biology - cheryl d. helgason, cindy l. miller
Ngày tải lên :
08/04/2014, 12:50
... enabling every researcher using human cell lines to easily verify cell line identity Key Words: Authentication; cross-contamination; PCR; DNA fingerprinting; false cell lines; VNTR; AmpFLP Introduction ... sample of cell lines using DNA fingerprinting and cytogenetic evaluation have shown a high incidence (approx 15%) of false cell lines observed among cell lines obtained directly from original investigators ... Basic Cell Culture Techniques 1 Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D Helgason Summary Cell culture is an invaluable tool for investigators in...
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Báo cáo hóa học: " Comparison of T-cell receptor repertoire restriction in blood and tumor tissue of colorectal cancer patients" docx
Ngày tải lên :
18/06/2014, 16:20
... ring cell carcinomas Cancer Res 1993, 53:3078-84 29 Weidmann E, Elder EM, Trucco M, Lotze MT, Whiteside TL: Usage of T -cell receptor V beta chain genes in fresh and cultured tumor-infiltrating ... comparing tissue with blood samples (48 vs 42 instead of 24 vs 18) Regarding the repertoire restriction in blood (healthy vs carcinoma patients) and tissue (unaffected colon mucosa vs carcinoma ... patients confirming the results of former studies investigating Vβ-repertoire restrictions in blood of patients with other tumor entities, which have been interpreted as indication for the induction...
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Báo cáo hóa học: "IL-2 as a therapeutic target for the restoration of Foxp3+ regulatory T cell function in organ-specific autoimmunity: implications in pathophysiology and translation to human disease" doc
Ngày tải lên :
18/06/2014, 16:20
... to be examined Page of 12 In humans, exposing CD4+ T cells to both IL-2 and rapamycin in vitro leads to an increase in the cellular frequency of FOXP3+ T cells, originating from nTreg cells and ... Teff cells, and in turn, driving the functional homeostasis of CD4+Foxp3+ Treg cells in the target organ IL-2 restores the Treg/Teff balance in T1 D IL-2 is important in instructing Treg lineage ... diabetes in NOD mice by quantification of autoreactive T cells in peripheral blood J Clin Invest 2003, 111:217-223 Eisenbarth GS, Kotzin BL: Enumerating autoreactive T cells in peripheral blood: ...
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Báo cáo sinh học: " Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" pot
Ngày tải lên :
18/06/2014, 22:20
... presented in the plus-sense RNA occur as nonpairing C/A bases in the minus-sense RNA Interestingly, in Puumala hantavirus, a hairpin-like structure formed by a highly conserved inverted repeat in the ... RNA from cells infected with TULV02 (line 1), on RNA from cells infected with the recTULV (line 2) and on the mechanical mixture of both RNA preparations (line 3) Lines 5–7: the corresponding products ... O: Intragenic recombination in rotaviruses FEBS Letters 1998, 427:183-187 Nayak DP, Chambers TM, Akkina RK: Defective interfering RNAs of influenza viruses: origin, structure, expression and interference...
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Báo cáo hóa học: " Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture" pptx
Ngày tải lên :
20/06/2014, 01:20
... AM: Differentiation of varicella-zoster virus ORF47 protein kinase and IE62 protein binding domains and their contributions to replication in human skin xenografts in the SCID-hu mouse J Virol ... was amplified from the rpOka-infected cells, but not from the pOka∆58-infected cells, indicating that the ORF58 gene product was not expressed in the rpOka∆58-infected cells As positive controls, ... L, Sommer MH, Heineman TC, Cohen JI, Kaneshima H, Arvin AM: The ORF47 and ORF66 putative protein kinases of varicella-zoster virus determine tropism for human T cells and skin in the SCID-hu mouse...
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Báo cáo hóa học: " Adaptation to cell culture induces functional differences in measles virus proteins" pdf
Ngày tải lên :
20/06/2014, 01:20
... IFN-inhibiting domains in the P and V proteins of Location of IFN-inhibiting domains in the P and V proteins of MeV P indicates P protein, V indicates V protein, NTD indicates shared amino terminal ... proteins, perhaps even proteins involved in avian IFN signaling IFN signaling inhibition by paramyxoviruses can be species specific, for example PIV5 cannot inhibit IFN-α/β signaling in murine cell ... V protein of MeV has been shown to regulate replication both in mini-genome assays and in infected cells [16,36] Mutational analysis characterized two domains involved in inhibition of mini-genome...
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báo cáo hóa học:" Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" ppt
Ngày tải lên :
20/06/2014, 04:20
... presented in the plus-sense RNA occur as nonpairing C/A bases in the minus-sense RNA Interestingly, in Puumala hantavirus, a hairpin-like structure formed by a highly conserved inverted repeat in the ... RNA from cells infected with TULV02 (line 1), on RNA from cells infected with the recTULV (line 2) and on the mechanical mixture of both RNA preparations (line 3) Lines 5–7: the corresponding products ... O: Intragenic recombination in rotaviruses FEBS Letters 1998, 427:183-187 Nayak DP, Chambers TM, Akkina RK: Defective interfering RNAs of influenza viruses: origin, structure, expression and interference...
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Báo cáo hóa học: " Resource allocation based on integer programming and game theory in uplink multi-cell cooperative OFDMA systems" ppt
Ngày tải lên :
20/06/2014, 22:20
... unilateral SINR increase in even time slot, and the capacity of a relay user is confined by the minimum SINR of the two kinds of time slots To get the balance in the two slots, the introduction ... c+d SINRn (2)(t − 1) k,m + SINRn (2)(t − 1) k,m + c+d · SINRn (2)(t − 1) k,m SINRn (2)(t) − SINRn (2)(t − 1) k,m k,m + SINRn (2)(t) + SINRn (2)(t − 1) k,m k,m + SINRn (2)(t − 1) k,m c+d SINRn ... a certain relay All of the orthogonal subcarriers are available in each cell, and each of them can exclusively be allocated to a certain user [9] Therefore, inter -cell interference originates...
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