cell and tissue culture in tree breeding

... Myeloma cell lines Hybridomas Cell line MRC-5 Cell line WI-38 Cell line Namalwa Cell line BHK-21 Cell line MDCK Cell line GH3 Cell line 293 Cell line !VCRE/!VCRIP References 1.3 Master and Working Cell ... References 1.2 Cell Lines for Biotechnologists Introduction Cell line CHO dhfrCell line Sf9 Cell line Schneider-2 Cell lines COS 1/COS Cell line NIH3T3 Cell line HeLa Cell line J558L Cell line Vero ... 1.7 1.8 1.9 CELL AND TISSUE CULTURE Identity Testing - An Overview Cytogenetic analysis Isoenzyme analysis DNA fingerprinting and DNA profiling References DNA Fingerprinting PRELIMINARY PROCEDURE:...

Ngày tải lên: 10/04/2014, 11:12

... or introductory level in a few weeks or months These include sterile technique, subculture of cells, freezing and thawing cells, cloning cells, measuring cell growth and viability, and starting ... lines being maintained in long-term culture Cell cultures coming into the laboratory, as frozen vials or as viable cultures, primaries, or established cell lines, should be quarantined in an incubator ... of Medicine, Houston, Texas INTRODUCTION TO CELL AND TISSUE CULTURE: Theory and Technique Jennie P Mather and Penelope E Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique...

Ngày tải lên: 11/04/2014, 09:48

... genetic and environmental factors in the variation of average ring density, and its components, and evaluating some implications for tree breeding MATERIALS AND METHODS The material, used in this ... traits and tree form [3, 7, 18–20, 22, 23, 27], and notwithstanding studies developed in France by Polge and Illy [36], Keller [26] Nepveu [30], and Chaperon et al [8], big gaps still exist in the ... obtained from a progeny test with 15 open-pollinated families, collected by Gomes [18] in different regions of Portugal (5 in Viana Castelo, in Mondim de Basto, and in Leiria), planted in 1979 in...

Ngày tải lên: 08/08/2014, 14:20

... mutation necessarily being located in the binding interface We selected eight amino acid residues in sTF for individual cysteine replacement and used the cysteines as handles to which we attached ... Residues E99 and L104 are located in the TF1 domain, and T197 is situated in the N-terminal portion of the TF2 domain, i.e close to the TF1 domain at the level of EGF1 in FVIIa in the sTF–FVIIa ... domain is in dark grey and the TF2 domain in light grey The Gla domain of FVIIa is shown in green; EGF1 in magenta; EGF2 in light blue; PD in dark blue The sTF residues highlighted in red are...

Ngày tải lên: 17/03/2014, 03:20

... species) Using baby-food jars for a growing chamber punching an air hole in the lid using foil as a contaminant barrier Baby-food jars make excellent and inexpensive growing chambers For the beginner, ... q Stage I establishment/germination (no hormone) Stage II multiplication (low auxin, high cytokinin) Stage III rooting (high auxin, low cytokinin) For home tissue culture, hormones are not necessary ... hormone that primarily stimulates cell division Examples are 6-(y,y,dimethylally-amino)-purine (2iP), Kinetin (K), and 6-benzylaminopurine (BAP) Toby Marsden defines the three classical stages...

Ngày tải lên: 20/04/2014, 16:35

... contained no cytokinin and had a lower level of mineral salts Cytokinins are strong inhibitors of adventitious rooting, and high-salt media had indirect inhibitory effects GD agar media and WPM ... embryogenic tissue within 7-9 weeks (fig 2) The embryogenic competence was maintained by embryogenic tissue subculture Embryogenic tissues cultured on modified SH medium containing cytokinin kept ... transferred into WPM containing a low concentration of cytokinin (BAP 0.1 mg·l and were cultured under ) -1 continuous light to induce conversion; 1218% of embryogenic cultures produced germinating somatic...

Ngày tải lên: 08/08/2014, 19:21

... Kornik and in Goldap, in 1976 using 2+2 seedlings raised in a nursery in Kornik The experiment includes half-sib progenies from 12 provenances from the North Eastern range of the spruce in Poland ... only, instead of provenances and families (within provenances), we obtain the following changes in variance components: decreases slightly and the expected gain is higher (ΔG At the individual ... was established in an incomplete block design Not only were the maternal trees selected at random, but the provenances were also a random choice of Forest Districts in the area and cone collections...

Ngày tải lên: 09/08/2014, 03:24

... (DO2) and consumption related to defects in microcirculatory perfusion and disturbances in cellular metabolic pathways, resulting in a deficit of oxygen extraction and use [2-4] Whether the tissue ... distributed in a wide variety of tissues in the cardiovascular system, including cardiomyocytes, vascular smooth muscle, and endothelial cells, and has been shown to mediate anti-apoptotic, anti-inflammatory, ... recovery period Tissue preparation The sham and the CLP mice were re-anaesthetized and placed on a heating pad, and core temperature was monitored using a thermocouple rectal probe and maintained between...

Ngày tải lên: 13/08/2014, 03:21

... experimentally, perhaps allowing for transduction of a larger number of the cells in a tissue, and increasing the likelihood of progenitor cell targeting when integrating vectors are used In summary, LCMV ... WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a generalized ... rises during embryonic stages, peaks in the newborn, and diminishes in adult tissues [8,9] Here we develop and investigate the use of LCMV envelope glycoproteins with high or low a-DG affinity for...

Ngày tải lên: 14/08/2014, 19:22

Ngày tải lên: 28/05/2016, 08:46

... culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods Induction of callus Fresh mountain ginseng ... above) Stock cell culture and culture condition Suspended cells of P vietnamensis were initiated through callus induction from the cultivated plant root [5] The cell line was maintained in MS liquid ... cut into sections of 2-10 mm and then were inoculated into MS solid medium (Murashige and Skoog, 1962) containing 30 g/L sucrose, mg/L 2,4-D, and 0.1 mg/L kinetin After month callus were induced...

Ngày tải lên: 12/02/2014, 10:20

... The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric acid (IBA), 0.1 mg/L of kinetin and 30 g/L sucrose The pH was adjusted to 5.8 before autoclaving Cells ... metabolite biosynthesis In cell suspension cultures of P ginseng and P notoginseng, a low initial concentration of phosphate in the medium sufficiently promoted both cell growth and ginsenoside accumulation ... [1] reported that NH4+ in the culture medium inhibited ginsenoside accumulation in P notoginseng cell suspension cultures and that maximum ginsenoside production was obtained when NH4+ was absent...

Ngày tải lên: 28/03/2014, 10:20

... ring cell carcinomas Cancer Res 1993, 53:3078-84 29 Weidmann E, Elder EM, Trucco M, Lotze MT, Whiteside TL: Usage of T -cell receptor V beta chain genes in fresh and cultured tumor-infiltrating ... carcinoma patients, unaffected colonic mucosa, carcinoma tissue) one has to keep in mind the differences in sample sizes and applied tests The highly significant difference in CD between blood and ... size of tissue samples as we used for comparing tissue with blood samples (48 vs 42 instead of 24 vs 18) Regarding the repertoire restriction in blood (healthy vs carcinoma patients) and tissue...

Ngày tải lên: 18/06/2014, 16:20

... extraction and running buffers on staining intensity and resolution J Trop For Sci 3, 111-122 Loomis ND (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles ... enstained following Cheliak and Pitel and Liengsiri et al (1990a) with minor modifications zymes (1984) were Effect of tissue storage conditions and freezing periods In this experiment, tissue ... Genetic inference of enzyme variants in The resolving power and the staining intensi- evaluated for each enzyme and pH conbad resolua 6-step score (0 tion, poor, average, good, very good, excellent)...

Ngày tải lên: 08/08/2014, 23:22

... Germany) Cell aggregate cultures were ground up in Tri-Reagent using Molecular Grinding Resin (Geno Technology Inc, St Louis, MO, USA) For gene Results HIF-1α, PHD2 and P4Hα(I) expression in inner and ... region In order to determine whether these differences in expression in vivo were reflected in different responses inherent in the cells present in inner and outer meniscus, we isolated the cells ... factor (HIF)-1α and downstream target genes that are involved in the adaptive response of cells and tissues to low oxygen tension were expressed differently in cells in the outer and inner regions...

Ngày tải lên: 09/08/2014, 10:20

... functions in tissue regeneration, tissue remodelling and cancer progression In tumors and ischemic tissues EPCs have a direct structural role of differentiating into mature endothelial cells and an indirect ... observed a cyclical increase in EPC mobilization following a rise in estrogen and VEGF levels during menstrual cycling in uterine tissue [50,51] Recently, the role of estrogen in tumor induced neovascularization ... generated by ischemic tissue and tumor cells including GM-CSF and VEGF, which play a critical role in mobilization of EPCs to ischemic tissues and tumors Hypoxia in tumors and ischemic tissues mediate...

Ngày tải lên: 10/08/2014, 21:23

... working in the cervical model, working designing, and authoring E.N.G-T participated in the analysis and interpretation of the data and in writing and revising this report C.R-P participated in ... of the data, and in the writing and revision of this report L.I-T participated in designing the in vivo cervical tissue model and helped analyze and interpret the results H.H.L and L.I-T made ... AgNPs After 48 h of incubation in a 37°C humidified incubator, the tissues were washed, embedded in paraffin, and stained with hematoxylin and eosin b) Cervical explants in the upper Transwell...

Ngày tải lên: 11/08/2014, 00:22

... cells Interestingly over the 48 h incubation there was a marked increase in 11b HSD-1 gene expression in nonfat but not in fat cells [Figure 4] The increases in IL-8 release and mRNA in non-fat cells ... mesh and the SV cells were combined to obtain the nonfat cells One gram of adipose tissue explants, the nonfat cell fractions or fat cells obtained by digestion of g of tissue were incubated in ... The initial ratios of IL-8 and HIF1a in nonfat cells to fat cells was 9.2 and 4.6-× while that of 11b HSD1 was 0.25 indicating that there is 4-fold more 11b HSD1 in fat cells than in nonfat cells...

Ngày tải lên: 11/08/2014, 08:22

... Changes in gene expression during passaging (a) The changes in gene expression increase substantially during passaging of the cells in all fibroblast cultures In addition, the variations and differences ... doubling time of the population over the passaging time of the cells to evaluate changes in cell growth rates in comparison to earlier passages Materials and methods Synovial tissue and cell culture ... was involved in experimental design and organization, paper design and structure All authors read and approved the final manuscript Figure Cell doubling rates (in days) during cell culture passages...

Ngày tải lên: 12/08/2014, 12:20

... war that War Land on the Eastern Front had ushered in modernity, breaking traditions, altering and recasting old certainties, and overthrowing empires In these investigations, ‘ culture ’ was ... strikingly, after suppressing the 1863 revolt, Russia banned Coming to war land 37 printing of Lithuanian in Latin letters, seeking thus to remove Lithuanians from Polish inXuence, bringing them ... valleys, a twilight of uncertain shapes and living forms Finally, rain would begin again Winters were harsh, as Siberian winds brought inWnities of snow to cover the land One awed oYcial felt ‘‘deep...

Ngày tải lên: 21/09/2012, 11:02