cell and tissue culture and plant regeneration

... Introduction Cell line CHO dhfrCell line Sf9 Cell line Schneider-2 Cell lines COS 1/COS Cell line NIH3T3 Cell line HeLa Cell line J558L Cell line Vero Myeloma cell lines Hybridomas Cell line MRC-5 Cell ... of cells and their first subculture Use of haemocytometer5 for the calculation cell densities of Cell culture §ubculture of suspension cells Subculture of adherent cells Rejection of failed cell ... 4.3 xvi CULTURE CELL AND TISSUE Angela Martin National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland Section:...

Ngày tải lên: 10/04/2014, 11:12

... INTRODUCTION TO CELL AND TISSUE CULTURE: Theory and Technique Jennie P Mather and Penelope E Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique Jennie P Mather and Penelope ... to cell and tissue culture : theory and technique / Jennie P Mather and Penelope E Roberts p cm.¡ª (Introductory cell and molecular biology techniques) Includes bibliographical references and ... mammalian cell in vitro The Practice and Theory of Tissue Culture This book is meant to serve as an introduction to cell culture both for students who have little or no experience of cell culture and...

Ngày tải lên: 11/04/2014, 09:48

... treatment involved 30-60 explants and was repeated twice Culture media and conditions Organ cultures Explants were cultured on modified GresshoffDoy (GD) medium (Gresshoff and Doy, 1972), BTM (Chalupa, ... embryogenesis and plant regeneration in Picea, Quercus, Betula, Tilia, Robinia, Fagus, and Aesculus Commun Inst For Cech 15, 133-148 V (1987b) European hardwoods In: Cell and Tissue Culture in Forestry ... proliferation Biol Plant 30, 414-421 318 Chalupa V (1990c) Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L) and linden (Tilia cordata Mill) Plant Cell Rep...

Ngày tải lên: 08/08/2014, 19:21

... remaining stem cells for tissue regeneration and repair? In conclusion, MSCs derived from adult tissue present an exciting progenitor cell source for applications of tissue engineering and regenerative ... stem cells in order to identify core ‘stem’ genes and to examine supportive tissue to understand what genes and pathways are involved not only in stem -cell differentiation, but also in stem -cell ... engineering and regeneration [134] Mesenchymal stem cells versus embryonic stem cells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can...

Ngày tải lên: 09/08/2014, 01:21

... contains a population of cells ("dentin pulp stem cells"; DPSCs) with stem cell- like properties such as self-renewal and the ability to differentiate into adipocytes and neural-like cells [36], but not ... the "gold standard" in periodontal regeneration Cellular mixed stratified cementum (CMSC), found in the apical and furcation regions of molars areas, consists of a mixture of AEFC and cellular intrinsic ... progenitor cells within the PDL need to differentiate into several cell types (i.e osteoblasts, cementoblasts, fibroblasts, and endothelial cells) These cells must subsequently synthesize and release...

Ngày tải lên: 11/08/2014, 23:22

... isolates WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a ... cytotoxicity, and limited tropism Able to withstand ultracentrifugation, LCMV glycoproteins yield MLV and HIV vector titers similar to the widely used amphotropic and VSV-G envelopes [1-3] Stable cell ... investigate the expression of a- and b-DG in the liver at pre- and postnatal time points and document the in vivo tissue tropisms of these LCMV pseudotypes in neonatal and adult mice We hypothesized...

Ngày tải lên: 14/08/2014, 19:22

... catalase–peroxidases and APXs have been outlined [15], and a dendrogram of 29 lignin and manganese peroxidases have been presented [16] The present study should contribute to our understanding of the ... Blosum 30 protein–weight matrix; helix and strand gap penalty, 4; and loop gap penalty, Finally, areas of extensive gaps (i.e longer than 10 amino acid positions and present in more than 90% of sequences) ... and Chlamydomonas reinhardtii) and all thylakoid APXs from higher plants analysed here An important observation is the occurrence of two different APXs from the same organism (e.g thylakoid and...

Ngày tải lên: 19/02/2014, 16:20

... negative than Yw (cell) (–0.244 MPa), and water will move from the turgid cell to the solution As water leaves the cell, the cell volume decreases As the cell volume decreases, cell Yp and Yw decrease ... loss and the threat of dehydration A major difference between plant and animal cells that affects virtually all aspects of their relation with water is the existence in plants of the cell wall Cell ... water loss by the cell and consequent increased concentration of cell solutes Contrast this with the volume changes of a cell lacking a wall Measurements of cell water potential and cell volume (see...

Ngày tải lên: 20/02/2014, 01:20

... in plant cells Annu Rev Plant Physiol Plant Mol Biol 46: 189–214 Bradley, D J., Kjellbom, P., and Lamb, C J (1992) Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell ... contact with the celluloses and hemicelluloses already present The mechanical rigidity of lignin strengthens stems and vascular tissue, allowing upward growth and permitting water and minerals to ... feeding, the cell contents of different tissues mix and HCN forms Cyanogenic glycosides are widely distributed in the plant kingdom and are frequently encountered in legumes, grasses, and species...

Ngày tải lên: 07/03/2014, 20:20

... miR-133a and miR-133b inhibited cell Esophageal Cancer – Cell and Molecular Biology, Biomarkers, Nutrition and Treatment proliferation and invasion in ESCC MiR-200a has been linked to the etiology and ... therapy [4] Growth factors regulate growth and development of cells They might be supplied by distant glands and tissues, neighboring cells, or in situ by tumor cells themselves Thus growth factors ... resulting in cell morphology change and increased invasiveness [33] 24 Esophageal Cancer – Cell and Molecular Biology, Biomarkers, Nutrition and Treatment Fig Growth factors, Ras signaling and cell...

Ngày tải lên: 08/03/2014, 00:20

... 1966 Sand and water culture methods used in the study of plant nutrition.Technical communication 22, Common wealth Agriculture Bureaux England: 399–463 Hoagland D and Arnon D I 1950 The water culture ... accumulation in root and aerial parts of the plant in both the sand and soil cultures (p≤ 0.03) Relatively higher increases in Cd accumulation were observed in plants grown in sand (Mann Whitney ... acid and Cd-aspartic acid] and are depicted in Figure Discussion The distribution of Cd in the plant tissues (root, shoot and fruit) indicated that 70–80% of the Cd was retained in roots and only...

Ngày tải lên: 15/03/2014, 23:22

Ngày tải lên: 15/03/2014, 23:56

... n.d n.d and pCH2pppA appear to be the strongest inhibitors of both the human and plant enzymes The Ki values estimated for the human enzyme, 1.6 and 2.3 nm, respectively, were over 30- and 20-fold ... [2], and the dinucleoside 5¢,5¢¢¢-P1,Pn-polyphosphates (NpnN¢s, where N and N¢ are 5¢-O-nucleosides and n represents the number of phosphate residues in the polyphosphate chain that links N and ... hydrolase by p4A and ppCH2ppA with that by ATP (p3A) and pCH2ppA, both compounds truncated by one phosphate (and one negative charge) relative to the nucleoside tetraphosphates Both ATP and its b,c-methylene...

Ngày tải lên: 16/03/2014, 14:20

... helper cells The cytotoxic CD8+ T cells then affect beta -cell damage by releasing perforin and granzyme, and by Fas-mediated apoptosis In this way, macrophages, CD4+ T cells, and CD8+ T cells ... infiltrate showed that most cells corresponded to T cells, with T cytotoxic/suppressor cells being most abundant, although helper CD4+ T cells and NK cells Immunologic and Genetic Factors in Type ... and Stem Cell and Other Therapies disease initiation method, with a single and defined myelin peptide allows for the observation and measurement of changing T cell specificities over time (Vanderlugt...

Ngày tải lên: 16/03/2014, 21:20

... Animal and Plant Diseases cultures are more convenient and less expensive than eggs and lab animals Cell cultures are suitable to be examined microscopically for evidence of viral proliferation, and, ... tissue cultures Viral disease diagnosis has relied on the isolation of viral pathogens in cell cultures This method is often slow and requires considerable technical expertise and equipment Cell ... detection and identification of many human viral pathogens Viruses reach high titres when grown within susceptible cells, and culture tubes are convenient to manipulate Cell cultures in cell monolayer...

Ngày tải lên: 23/03/2014, 16:21

... hypoxia on cancer cells and endothelial cells within a tumour Fig Schematic representation of the effects of HIF-1 activation under intermittent hypoxia on cancer cells and endothelial cells within ... Durand & AquinoParsons that blood flow decreases could transiently arrest the division of tumour cells in S-phase [62] These cells are the main targets of chemotherapy, and the arrest of their cell ... tumour cell dissemination pathway in the body, tumour blood vessels supply to cancer cells the oxygen and nutrients essential for their survival and proliferation In the absence of angiogenesis and...

Ngày tải lên: 30/03/2014, 04:20

... is then dialyzed, titered, and stored in aliquots at –70°C Materials 293 Cells (or derivatives such as C7 or C7-cre cells) Tissue culture dishes (60, 100, and 150 mm) and 24-well plates DMEM + ... equal volume of 2X HBS and equilibrate it, the PBS, and the culture medium to 37°C Viral CPE is considered 100% when all cells are round and mostly detached from the tissue culture dish Because ... replication by the C7 cells and will ultimately be produced at much lower titers Seed a 60-mm tissue culture dish with approx 106 C7 cells in mL of DMEM + FBS Incubate until these cells reach approx...

Ngày tải lên: 10/04/2014, 11:11

... between the cells and the host tissue Important advances that use primary cells, stem cells, and cell lines that withdraw from the cell cycle are NEUROTROPHIC FACTORS AND GENE THERAPY 215 now the ... tissue and therefore remain as isolated tissue masses These types of cells are foreign to the brain and we not know the longterm consequences of these foreign cells within the CNS The ideal cells ... Progenitor cells refer to cells with a more restricted potential than stem cells, and precursor cells refer to cells within a given developmental pathway The presence of neural stem cells in the...

Ngày tải lên: 10/04/2014, 22:11

... sizes of the fragments/bands You may draw a line across the band of your query and the size marker to the estimation How many fragment(s) of DNA were generated by RE1 and RE2? And what is/are the ... Cover the gel lid and connect the power supply to run at 100 volts for 20 Please be careful and not touch any part of the electrodes and power supply Page of IBO2012 SINGAPORE CELL & MOLECULAR ... fragments of 650 bp and 350 bp The same piece of kbp DNA was digested with another enzyme RE4, releasing fragments of 800 bp and 200 bp And when this kbp DNA was digested with RE3 and RE4 together,...

Ngày tải lên: 03/06/2014, 17:36

... ANSWER SHEET CELL & MOLECULAR BIOLOGY Q1.7 (1 point) Answer: Q1.8 (1 point) Larger Q1.9 Smaller Same size (1 point) Answer: Q1.10 (2 points) a b c Page of IBO2012 SINGAPORE CELL & ... points × = 10 points) a Q1.4 b b (20 points) Page of IBO2012 SINGAPORE PRACTICAL TEST ANSWER SHEET CELL & MOLECULAR BIOLOGY Q1.5 (10 points) Attach photograph of gel here Q1.6 (1 point × = points) ... IBO2012 SINGAPORE PRACTICAL TEST ANSWER SHEET CELL & MOLECULAR BIOLOGY Task (100 points) Gene mapping by restriction endonuclease digestion of...

Ngày tải lên: 03/06/2014, 17:41