cd47 iap in human articular cartilage and extracted chondrocytes
Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... degrades the essential amino acid tryptophan Murine macrophages and DCs expressing IDO inhibit T-cell proliferation or induce T-cell apoptosis in vitro and in vivo [17] Munn and colleagues reported ... associated with induction of T-cell tolerance and immune inhibition in the induction of oral tolerance in the murine CIA model We examined the change in the expression of IDO in CD11c+ DCs of ... expression and inhibit T-cell proliferation In this regard, it will be interesting to investigate IDO expression in different subsets of DCs from tolerized mice and to characterize their role in the induction...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, mM L-glutamine, 100 U/ml penicillin, and 0.1 mg/ml streptomycin The ... 1640 medium containing 1% heat-inactivated autologous serum, 1000 U/ml recombinant human GM-CSF, 500 U/ml recombinant human IL-4, and 10 ng/ml recombinant TNF-α (Becton Dickinson) [30] On day ... assay Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium Colorectal carcinoma cell lines (COLP-2 and COLM-6) were maintained in TIL Media I medium...
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báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx
... Mellor AL: Indoleamine 2,3-dioxygenase and tumor-induced tolerance J Clin Invest 2007, 117:1147-54 Astigiano S, Morandi B, Costa R, et al: Eosinophil granulocytes account for indoleamine 2,3-dioxygenase-mediated ... specific inhibitor of IDO at concentration of mM in the co-culture system composing of CHO/IDO cells and CD3+T cells to elucidate the regulatory effect of IDO both in promoting apoptosis and increasing ... and LY participated in the sequence alignment FW and WY performed the statistical analysis JL and XR conceived of the study, and participated in its design and coordination All authors read and...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... Iron in the enzyme was reduced to Fe2+ with hydroxylamine-HCl and then measured using o-phenanthroline [23] 4-Amino-3hydroxybenzoic acid in the growing culture was determined using a diazo coupling ... correlated with an increase in cell density and in protein content 2,5-Pyridinedicarboxylic acid (Fig 4a) in the culture broth in which strain 10d grew was not detected by HPLC The strain could not ... growth experiments, strain 10d was grown in basal medium containing 4-amino-3-hydroxybenzoic acid (1.2 gÆL)1) and yeast extract (0.025 gÆL)1) and in basal medium containing only yeast extract...
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Báo cáo khoa học: The role of residue Thr249 in modulating the catalytic efficiency and substrate specificity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1 ppt
... chain makes the closest contact between the residue and the substrate Lines with round ends indicate stacking between the ring of the substrate and His240 in DHBD (A), His248 in HPCD (B), and ... chain of residue 249 caused by mutation of threonine to serine, alanine and glycine creates a new cavity (subsite 3¢) adjacent to CH atoms at position 6, resulting in the reduction of steric hindrance ... (Table 1) The region facing positions and of the catechol ring is mainly formed by a loop containing residues 246–249 in C2,3O (240–243 in DHBD and 248–251 in HPCD) (Fig 2) Multiple alignments...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... between clinical responses and T regs in cancer patients undergoing hTERT-pulsed DC vaccination This novel finding is of substantial significance in hTERT-based immunotherapy in particular, and cancer ... transiently eliminating T regs Phase II/III clinical trials, involving stage IV cutaneous T cell lymphoma and Non-Hodgkin's lymphoma patients, has demonstrated intravenous denileukin diftitox inducing disease ... partial clinical responses following vaccination with the vaccination time point Patient PSA baseline levels (μg/L) Vaccination time point (V) PSA Post-response (μg/L) Vaccination time point (V)...
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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps
... acts as an interleukin-2 sink and hinders the autocrine/paracrine stimulation of TE cells 23 Arthritis Research & Therapy Vol No Fehérvári and Sakaguchi ably an as yet uncharacterised inhibitory ... them into phenotypical and functional TR cells capable of effectively suppressing both in vitro and in vivo [26,27] Thus Foxp3 seems to be a ‘master gene’ controlling the normal development and/ or ... cytokines such as IL-10 and TGF-β (reviewed in [49]) The situation with naturally occurring TR cells is not nearly so clear-cut and despite intense interest remains strangely inconclusive Potential...
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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
... A: Interleukin-10 and the interleukin-10 receptor Annu Rev Immunol 2001, 19: 683-765 Steinbrink K, Graulich E, Kubsch S, Knop J, Enk AH: CD4(+) and CD8(+) anergic T cells induced by interleukin-10-treated ... both foreign antigen and self-antigen [56], including TR cells specific for alloantigens [57] TR cells in humans, as in mice, express FoxP3 [58], and individuals with a mutation in the FoxP3 gene ... differentiating into TR cells following activation are derived from a separate lineage of CD4+ T cells, and whether their induction is a result of the initial activation early in the inflammatory...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
... Weiner HL, Hafler DA: Induction of circulating myelin basic protein and proteolipid protein-specific transforming growth factor-beta1secreting Th3 T cells by oral administration of myelin in ... activity in some experiments [14] The principal difference between the cytokine-induced CD4+ CD25+ cells and the murine and human positively selected CD4+ CD25+ cells that are predominantly found in ... the predominant TGF-β effect on T cells in this fraction is the generation of ‘professional’ regulatory T cells remains to be determined Our finding that both IL-2 and TGF-β are critical in the...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
... islets and pancreatic lymph nodes in inflammation-induced diabetes [29], in chronically inflamed skin in a Leishmania infection model [30], and in the mucosa and mesenteric lymph nodes in inflammatory ... Netherlands) in RPMI 1640 for at room temperature After washing, × 106 cells were injected intravenously Twenty-four hours later single cell suspensions were prepared from the draining and nondraining ... measured before injection and 24 and 48 hours later using a vernier caliper (Kroeplin) Proliferation assay and ELISPOT analysis Single cell suspension from spleens and lymph nodes (inguinal, popliteal,...
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Báo cáo y học: "β Transforming growth factor-β-induced regulatory T cells referee inflammatory and autoimmune diseases" potx
... cell proliferation and cytokine production, reining in Th1 and/ or Th2 immunity Without adequate intercession by Treg, Th1- or Th2-dominated responses may become pathogenic and/ or function of ... responses with minimal pathology In human infectious, neoplastic, and autoimmune diseases, Treg activities often mirror those in murine systems Numbers of Treg are reportedly reduced in human autoimmune ... associated with the latent TGF-β1-binding protein, forming a large latent complex thought to serve as a tether for binding proteins and matrix molecules [31] In this configuration, TGF-β is not...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
... decreases the infiltrate in joints Furthermore, RA patients relapse shortly after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation and the reinstatement of ... follow in humans because not all autoantigen-specific T cells seem to be effective in treating an ongoing autoimmune disease as described in the T1D model [22] Therefore in humans such a prescreening ... Elferink DG, Bakker AM, Miltenburg AM, Huizinga TW, de Vries RR, Toes RE: Functional regulatory immune responses against human cartilage glycoprotein-39 in health vs proinflammatory responses in...
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Báo cáo y học: "Regulatory T cells in systemic lupus erythematosus: past, present and future" pot
... for interleukin in Foxp3-expressing regulatory T cells Nat Immunol 2005, 6:1142-1151 β Marie JC, Letterio JJ, Gavin M, Rudensky AY: TGF-β1 maintains suppressor function and Foxp3 expression in ... IL-17-producing cells [29,70] iTregs in mice induced ex vivo with IL-2 and TGFβ are resistant to this conversion [31], but in humans this has yet to be demonstrated The cytokine and gene expression ... surface [80], TNF family proteins [81], and adenosine and its receptors [82,83] downhill and fatal, whereas human disease is cyclic and characterized by exacerbations and remission Moreover when...
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Báo cáo y học: "Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells" doc
... WJ, Scheld WM: Recombinant human interleukin-1 induces meningitis and blood-brain barrier injury in the rat: characterization and comparison with tumor necrosis factor J Clin Invest 1991, 87:1360-1366 ... of JEV wild strain infection by testing the interaction of JEV and DCs in vivo and in vitro Carrasco et al., [2004] discovered that CSFV could infect and replicate in monocyte and myeloid-derived ... alteration of phenotype and function of the JEV P3-infected DCs both in vitro and in vivo Our results indicated that JEV P3 severely infected DCs in vitro and in vivo, and the infection with JEV impaired...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
... Servant, R Zollinger, SI Bogiatzi, P Hupé, E Barillot, V Soumelis, A critical function for transforming growth factor-β, interleukin 23 and proinflammatory cytokines in driving and modulating human TH-17 ... cells from MPE and blood were determined by flow cytometry after surface staining or intracellular staining with anti -human- specific Abs conjugated with either phycoerythrin or fluorescein isothiocyanate ... Tregs and Th17 cells in CD4+ T cells in MPE (Figure 1A, B, C) and peripheral blood With gating on CD4 + CD25high subset, we once again observed in the present study that a significant increase in...
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Báo cáo y học: "Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells" pot
... directly binding to its sugar export site [34,35] In the presence of CytB, H2RBDEGFP binding was significantly decreased, strongly suggesting that CytB binding to Glut-1 directly inhibits binding of ... H2RBDresults in Glut-1 expression and concomitant glucose uptake in CD4 and CD8 lymphocytes: Induction of H1RBD and TCR stimulation binding TCR stimulation results in Glut-1 expression and concomitant ... detection in both quiescent CD4 and CD8 T cells and increased by >20-fold in both subsets following stimulation (Fig 3B) Notably, the increase in glucose uptake was equivalent in the CD4 and CD8...
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Báo cáo y học: "Signatures of human regulatory T cells: an encounter with old friends and new" pdf
... X-linked XLP, RA, MS S100A4 S100 calcium binding protein A4 RA PIM1 Pim-1 oncogene ID2 Inhibitor of DNA binding FHIT Fragile hisT1Dine triad gene reports SLAMF1 Diabetes RNA binding motif, single ... as being upregulated in the naturally occurring TReg cells from our individual donors S100A4 is a member of the S100 family of proteins containing two EF hand calcium binding motifs EF-hands ... penicillin/streptomycin, and nonessential amino acids (PAA Laboratories, Linz, Austria) Human peripheral blood was obtained after informed consent had been obtained, in accordance with institutional...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... vaccine containing OVA and Fc fusion gene targeting DCs, the murine OVAcDNA was amplified from OVA-pcDNA3.1 plasmid by polymerase chain reaction (PCR), spliced and then cloned into pMIgV containing ... both activating and inhibitory FcRs, and the balance between activating and inhibitory signaling will determine whether uptake of immune complexes results in naive T cell activation and protective ... assessed for morphology and cellular infiltration using haematoxylin and eosin (H&E) staining The degree of cellular infiltration was scored as described previously [6] Inflammatory changes were...
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Tài liệu Lab 4.2.3 Suspending and Disconnecting Telnet Sessions docx
... Login to Router and verify the connection to Router a Login to the Gadsden router b Verify the connection between the two routers Ping the serial interface of the Birmingham router If the ping ... Copyright 2003, Cisco Systems, Inc Router Interface Summary Router Ethernet Ethernet Serial Serial Interface Model Interface #1 Interface #2 Interface #1 Interface #2 #5 800 (806) Ethernet ... of interface even though a specific router may contain one An example of this might be an ISDN BRI interface The string in parenthesis is the legal abbreviation that can be used in IOS command...
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Tài liệu Lab 4.2.3 Suspending and Disconnecting Telnet Sessions doc
... Login to Router and verify the connection to Router a Login to the Gadsden router b Verify the connection between the two routers ping the serial interface of the Birmingham router If the ping ... Copyright 2003, Cisco Systems, Inc Router Interface Summary Router Ethernet Ethernet Serial Serial Interface Model Interface #1 Interface #2 Interface #1 Interface #2 #5 800 (806) Ethernet ... of interface even though a specific router may contain one An example of this might be an ISDN BRI interface The string in parenthesis is the legal abbreviation that can be used in IOS command...
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