cd4 t cells in the presence of atp
Báo cáo y học: " Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques" potx
Ngày tải lên :
13/08/2014, 05:21
... subtracting the percentage of GFP-positive cells obtained after treatment with a saturating dose of AZT, from the percentage of GFP-positive cells obtained in the absence of AZT treatment Fluorescence ... differentiation of CD34+ cells[ 63], possibly due to a delay in the integration of the transgene during differentiation, resulting in the formation of clusters of GFP-positive cells within a single ... Kootstra et., al showed that the viral determinant involved in postentry restriction of HIV-1 replication in simian cells is located at or near the cyclophilin A (CyPA) binding region of the...
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Báo cáo khoa học: "Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells" pdf
Ngày tải lên :
07/08/2014, 20:23
... sesaercni yltnacifingis GPA taht dewohs yduts sihT sMB fo ytivitcaerolla eht no tceffe sti ro snoitaretla cipytonehp eht no noitamrofni elttil si ereht ,]02[ seitivitca evitcetorpoidar dna yrotaludom ... deniats ehT lortnoc eht sa desu saw bAm dehctam -epytosi delebal-EP ro -CTIF rehtiE gniniats tcerid rof desu saw bA-I esuom-itna delebal-CTIF gniniats tceridni rof )ASU ,secneicsoiB DB( nidivatperts-)EP( ... si ti ,eroferehT )B1 giF( noitaidarri ammag fo ecnesba ro ecneserp eht ni sMB lortnoc eht naht rehgih yltnacifingis saw sMB detaert-GPA fo ytilibaiv eht taht dewohs yassa TTM noitaidar ammag htiw...
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Báo cáo khoa học: "Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity" doc
Ngày tải lên :
07/08/2014, 23:22
... measurements Annexin V-FITC/PI staining and rhodamine 123 staining were used to confirm the protective effects of fucoidan on the viability of irradiated BMCs (Fig 2) With the annexin V-FITC/PI staining ... demonstrate that fucoidan increases BMC viability following irradiation These effects may be the result of apoptotic inhibition in irradiated BMCs Fucoidan treatment alters the expression of apoptosisrelated ... cell types whereas it enhances the survival of other cell types of BMCs The effects of fucoidan on specific cell types of BMCs need to be investigated in future work Our findings indicated that...
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Báo cáo y học: "Quantitative ultrasound can assess the regeneration process of tissue-engineered cartilage using a complex between adherent bone marrow cells and a three-dimensional scaffold" docx
Ngày tải lên :
09/08/2014, 06:22
... below, left, and right of the center The percentage maximum magnitude (the maximum magnitude of the measurement area of the operated knee divided by that of the intact cartilage of the opposite, nonoperated ... expected to become one of the quantitative indexes of cartilage regeneration therapy Competing interests The author(s) declare that they have no competing interests Authors' contributions KH ... operated knee, divided by that of the intact cartilage of the opposite, nonoperated knee *P < 0.05 on the nonparametric Mann–Whitney U test Histological findings In the histological findings, the...
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Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
Ngày tải lên :
12/08/2014, 17:21
... results suggest that the overexpression of genes that take part in the antigen presentation pathway and IFN signaling contribute to the pathogenesis of RA Our results also suggest that the underexpression ... are pleiotropic in their biological activities and that they interact with each other in highly sophisticated networks Along these lines, the effects of IFNb treatment on arthritis were reviewed ... recruited the patients for this study, and analyzed the clinical data of the patients TO and NN made substantial contributions to the conception and design of the experiments, and analysis and interpretation...
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Báo cáo y học: "Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 07:20
... β-actin by real-time quantitative PCR The data are expressed as the ratio of the mRNA copy numbers to those of β-actin The data are representative of two different experiments ure 7, treatment of ... effects in the treatment of RA [4] On the other hand, increasing attention has been paid to the role of bone marrow abnormalities in the pathogenesis of RA In this regard, we demonstrated that RA ... (expressed as the ratio of the mRNA copy numbers to those of β-actin) in bone marrow CD34+ cells from six patients (three rheumatoid arthritis patients and three osteoarthritis patients) Statistical...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
Ngày tải lên :
25/10/2012, 11:18
... apply stem cell transplantation in the treatment of femoral necrosis [32-37] However, intravenous injection of stem cells as a therapeutic strategy is less investigated in the treatment of femoral ... Another concern of intravenous transplantation of MSCs is whether the MSCs can migrate into and proliferate in the target tissues, which is the basis of therapeutic effects of MSCs In adults, ... migrated into almost all organs, and were uniformly distributed in the brain, lungs, heart, kidney, intestine, hip joints and other organs at 24 h after transplantation At 48 h after transplantation,...
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báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot
Ngày tải lên :
18/06/2014, 15:20
... 2-D pattern of the silver stained gel image of the master gel image 2-D pattern of the silver stained gel image of the master gel image 2-D pattern of the silver stained gel image of a master gel ... involved in the formation and maintenance of the histo-architecture Plasmonogen activator inhibitor-2 (PAI-2) Involved in the regulation and inhibition of binding between urokinase-type plasminogen ... image containing the spot information of all investigated samples For protein identification, each gel image was matched to the previously 130 identified spots of the gastric carcinoma cell line EPG85-257...
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báo cáo hóa học:" A practical approach for the validation of sterility, endotoxin and potency testing of bone marrow mononucleated cells used in cardiac regeneration in compliance with good manufacturing practice" docx
Ngày tải lên :
18/06/2014, 15:20
... sterility testing, the detection limit represent the lowest bacterial concentration in the inoculums that the system can evidence The specificity of the system represent its ability to detect ... test system is based on the kinetic chromogenic LAL-test that is based on the cleavage of a synthetic substrate by an enzyme produced in the reaction of the lysate in the presence of endotoxin The ... the direct inoculation method requires the sample to be inoculated directly into test media For the latter, sample is incubated for 14 days in the test media It is important to determine if the...
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Báo cáo hóa học: "Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion" doc
Ngày tải lên :
18/06/2014, 16:20
... establish the efficacy of the method Since the net “healing” capacity of BMSCs is difficult to determine, imaging of transplanted stem cells is crucial in order to investigate the attitude of the ... http://www.translational-medicine.com/content/9/1/12 Figure Intraoperative view showing clinical application of stem cells into the failing heart with multiple targeted injections following device insertion Note the outflow ... support cannot lead to repopulation of the infarcted tissue with contracting cardiomyocytes This fact could explain the inability to wean mechanical support in patients with ICM [2] Induction of...
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báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
Ngày tải lên :
20/06/2014, 01:20
... selected in term of bioactivities of osteoblasts and endothelial cells These outcomes would contribute to the construction of vascularized engineered bone in vitro and implantation in vivo http://www.josr-online.com/content/4/1/5 ... bioactivity of EPCs-originated ECs in biomaterials of bone graft is frequently neglected Therefore, the objective of this study was to evaluate the biocompatibility of the HA-PCL biomaterials to ... endothelial cells Our data showed that the addition of HA elevated NO production in comparison with the HA-free PCL scaffolds, suggesting that HA particles promoted functions of the endothelial cells...
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báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx
Ngày tải lên :
21/06/2014, 02:20
... pointed out that the AFM tip is going to be contaminated at the first touch and continue with the following touches, and this contamination can influence the next interaction of the tip with the cells ... variation in the oscillation amplitude of the cantilever, causing noise in the AFM images the contaminated tip collects, but such a contaminant may be removed from the apex by pushing the tip into ... from linearity was seen in the contact region of the immature BDMCs, indicating that the sample was not deformed by the probe Upon retraction, the adhesion force was detected, reflecting the absence...
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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
Ngày tải lên :
09/08/2014, 07:20
... modifying the characteristics of the MSC, which then R1313 act to favor the accumulation of immunocompetent cells into the joint An important feature revealed in the present study is that cells ... biostatistical analysis JS participated in the analysis of the data CJ participated in the design of the study and the analysis of the data DN participated in the design of the study and the analysis ... [10] Thus, although still to be demonstrated, MSC may play a pivotal role in the induction phase of arthritis by promoting the accumulation of immunocompetent cells into the joint To date, identification...
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Báo cáo y học: "Nurse-like cells reside in the synovial tissue and bone marrow in rheumatoid arthritis" doc
Ngày tải lên :
09/08/2014, 08:23
... 106 cells MC/car cells or Molt-17 cells were added to the culture After days of incubation, the culture supernatants were collected and the amount of each cytokine in the culture supernatant was ... intervention in RA Competing interests The authors declare that they have no competing interests References Acknowledgements The work reported here has been supported in part by a grant -in- aid from the ... antibody production by B cells, the capacity to protect lymphocytes from apoptosis, the ability to secrete large amounts of cytokines and chemokines that could promote the accumulation and activation...
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Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
Ngày tải lên :
09/08/2014, 10:23
... Kindle and colleagues reported that TNF activates endothelial cells and increases the attachment of OCPs to vascular endothelium in vitro They speculated that this could increase the ability of ... contribute to the accelerated OCP mobilization in inflammatory arthritis where systemic TNF levels are elevated Competing interests The authors declare that they have no competing interests Authors' ... malignancies The stem cells are then harvested from the blood as a source of stem cells to be returned to patients following chemotherapy or bone marrow transplantation Whether or not inflammatory cytokines...
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Báo cáo y học: "Rituximab therapy reduces activated B cells in both the peripheral blood and bone marrow of patients with rheumatoid arthritis: depletion of memory B cells correlates with clinical response" potx
Ngày tải lên :
09/08/2014, 14:22
... Treatment with rituximab does not affect the proportion of total CD4+ T cells, activated CD4+ HLA-DR+ T cells and CD4+ CD25+ T cells in the PB of RA patients that B cells are relatively refractory ... associ- ated with the persistence of memory B cells in both compartments Competing interests The authors declare that they have no competing interests Authors' contributions MN carried out the immunological ... immunological studies GK participated in the design and conduction of the study and drafted the manuscript PS participated in the design of the study, performed the statistical analysis of the data and...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
Ngày tải lên :
10/08/2014, 05:21
... these pleiotropic effects are the anti-apoptotic and pro-angiogenic properties of statins It has been demonstrated that statins could protect against ischemic injury of the heart and stimulate ... increased in the mice treated with either MSCs or simvastatin alone in comparison with the control group (p < 0.05) The combined administration of simvastatin and MSCs resulted in the highest ... were defined as TUNEL-positive and the percentage of apoptotic cells per total number of cells was determined by two independent blinded investigators To examine whether simvastatin enhance the release...
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báo cáo khoa học: "Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging" ppsx
Ngày tải lên :
11/08/2014, 00:22
... and fate of stem cells without postmortem histology, and the lack of tracking data represents a serious obstacle for the clinical use of cell therapy Thus, the development of sensitive, non-invasive ... legs; the right leg was injected with cells incubated with FeProt for hrs and left leg was injected with cells incubated with FeProt for 24 hrs There is no apparent difference in the intensity of ... with the cultures of days In the CTRL/7d and FeProt/7d groups, we plated half the amount of cells plated in the CTRL/3d and FeProt/3d groups but the cells approached confluence at days, and the...
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