cd4 t cells and t b cell collaboration

Báo cáo y học: " Importance of spliceosomal RNP1 motif for intermolecular T-B cell spreading and tolerance restoration in lupus" pptx

Ngày tải lên : 09/08/2014, 10:21

... containing 0.5% Tween (TBS -T) and 5% milk for h at room temperature (RT), and then incubated with rabbit antisera diluted 1:500 in TBS -T- milk for h at RT After washing, strips were incubated with ... on T- and B- cell reactivity to auto-antigens was not generalized to the total T- and B- cell response We found that P140-treated mice display normal capacity to successfully mount T- and B- cell ... hnRNP-A2 /B1 ) proteins Starting from the observation that sequences containing this RNP1 motif are often targeted by antibodies from lupus patients and mice, we hypothesized that the RNP1 motif could be...

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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps

Ngày tải lên : 09/08/2014, 07:20

... Another distinctive feature of this subfamily of TLRs is that, in humans, TLR7/8 and TLR9 exhibit a very limited cellular distribution and are detectable only in B cells and plasmacytoid dendritic ... autoreactive B cell precursors toward the follicular B cell pathway, activates T cells, and promotes development of myeloid dendritic cells (mDCs) Activated T cells direct isotype switching and affinity ... Mouse B cells, but not human B cells, also proliferate and differentiate into antibody-secreting cells when stimulated with lipopolysaccharide This requires full assembly of the multireceptor signaling...

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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc

Ngày tải lên : 14/08/2014, 18:20

... cytokine profile, endocytic and T cell activation abilities of the cells, again suggesting differences in the maturation status between mDCs and the DC models These observations have important ... [13], but no studies have compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and then attempt to relate this to the functional ... of both moDC and KG1 cells as mDC models, their similarity to peripheral blood DCs is yet to be properly defined This study aims to assess the suitability of both moDC and KG1 cells as model cells...

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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx

Ngày tải lên : 18/06/2014, 15:20

... provided about the origin of this process but it suggests that targeting T cells to some extent sustains the expansion of NK cells from PBMCs Interestingly; we demonstrated that like such antibodies, ... the fact that 3a-G1 inhibits CD4+ T cell proliferation without affecting NK cell one within PBMCs, we checked whether this activity could not be broadened to all T cells When stimulating T cell ... NK cells initially-obtained their name due to their natural cytotoxicity against tumor cells requiring no prior sensitization, unlike T cells [4] It is well established that the cytotoxicity...

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báo cáo hóa học:" Metabolic and anthropometric parameters contribute to ART-mediated CD4+ T cell recovery in HIV-1-infected individuals: an observational study" potx

Ngày tải lên : 20/06/2014, 08:20

... reconstitution after ART; and (2) if metabolic parameters could contribute to a predictive model for immune reconstitution that includes pre-ART viral, immune activation and CD4 + T cell counts The ... reconstitution attained after six months of continuous ART and contribute significantly to a predictive model of CD4+ T cell immune reconstitution Methods Study subjects We assessed 69 ART-naïve, ... obtained from the clinic record and by interview Written informed consent was obtained from all participants as per University of the Witwatersrand Ethics Committee- and Wistar Institute Institutional...

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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps

Ngày tải lên : 09/08/2014, 01:23

... distinctions still remain between the surface phenotype of TR and primed T cells, but they are more relative than absolute For example, although both primed T cells and TR cells express CD25, the latter ... expression was observed in CD25 CD4+ T cells; this appeared to be attributable to a small population of CD25 CD45 RBlowGITRhigh TR cells ([26], M Ono, manuscript in preparation) In addition, TR cells were ... antibodies against the IL-10 receptor, Asseman and colleagues were able to abrogate the normal IBD-preventative action of CD45 RBlow T cells [50] The same group was also able to show that CD45 RBlow...

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Báo cáo y học: "Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+ T cells from patients with rheumatoid arthritis" ppsx

Ngày tải lên : 09/08/2014, 01:24

... CD4+ T cells These findings indicate that CD4+ T cells become resistant to the inhibitory effect of IL-10 before migration into the inflamed ST, and suggest that this resistance may be attributable ... p-Tyr-STAT3 STAT3 IL-10 (ng/ml) 10 HC PB RA ST CD4+ T cells CD4+ T cells of IL-10-mediated (RA) STAT3 in CD4 T cells from (a)transcription (STAT) and from healthy controls (HC) patients with ... Briefly, the membrane was incubated with the antibodies (rabbit IgG) anti-STAT1 antibody, antiphosphorylated tyrosine 701 of STAT1 antibody, antiSTAT3 antibody, and anti-phosphorylated tyrosine...

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Báo cáo y học: "No evidence for an association between the -871 T/C promoter polymorphism in the B-cell-activating factor gene and primary Sjögren''''s syndrome" pptx

Ngày tải lên : 09/08/2014, 07:20

... anti-SSB autoantibodies (n [%]) 52 (32) Anti-SSA autoantibody only (n [%]) 54 (33.4) Anti-SSA and anti-SSB autoantibodies (n [%]) BAFF; and 5'-GCTGTGCTACGTCGCCCT-3' and 5'-AAGGTAGTTTCGTGGATGCC-3' ... circulating levels of BAFF protein leads to downregulation of BAFF transcription This might contribute to a transient dissociation between BAFF protein and BAFF mRNA levels Saturation of BAFF receptors ... specific pattern of autoantibody secretion either (T allele frequency in patients without autoantibody: 45%; in patients positive for anti-SSA autoantibody only: 48%; in patients positive for anti-SSA...

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Báo cáo y học: "Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation" doc

Ngày tải lên : 09/08/2014, 10:22

... lining (type A synoviocytes), with only scattered cells in the subintimal connective tissue These observations provide qualitative support to the quantitative studies on inflammatory cells obtained ... numbers of activated DCs in target tissues of autoimmunity [12,43], therefore, may result from the encounter between autoreactive effector T cells and immature DCs and these cells may exacerbate ... CD4+ CD1 1b+ , and CD4- CD1 1b+ (Figure 4b) Analysis of the CD4+ CD1 1b+ and CD4- CD1 1b+ subpopulations showed that both consist mainly of CD11c+ cells (Figure 4c) In the CD4+ CD1 1b+ subset, the proportions...

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Báo cáo y học: "Improving therapeutic HPV peptide-based vaccine potency by enhancing CD4+ T help and dendritic cell activation" docx

Ngày tải lên : 10/08/2014, 05:21

... more potent protective and therapeutic antitumor effects against TC-1 tumors in treated mice [22] Thus, the induction of CD4+ T help by employment of the PADRE strategy may be used in combination ... dissected Medium (5 ml) was added and the tumor was disintegrated by tweezers by rubbing against the mesh thus releasing the entrapped lymphocytes Cells were then filtered and treated with AKT lysing ... response in the tumor microenvironment, thus contributing to the destruction of the tumor Furthermore, the released tumor antigen, such as E7 may potentially be taken up by antigen-presenting cells, ...

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Báo cáo y học: "Immune restoration disease and changes in CD4+ T-cell count in HIV- infected patients during highly active antiretroviral therapy at Zewditu" ppsx

Ngày tải lên : 10/08/2014, 05:21

... pulmonary tuberculosis (PTB) and disseminated tuberculosis (DTB) between patients with and without IRD (P > 0.05) The interval between the start of HAART and the onset of Table Baseline characteristics ... treatment to alleviate symptoms of IRD There were eight deaths attributable to IRD and the causes of deaths were PTB, EPTB and DTB in 3, 3, and of them, in that order The mean ± SD baseline CD4+ ... initiation One hundred seventy (14.6%) of the study subjects developed IRD Table shows the baseline characteristics of patients with and without IRD The patients with IRD at HAART initiation were...

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báo cáo khoa học: "eneration of diffuse large B cell lymphomaassociated antigen-specific Va6/Vb13+T cells by TCR gene transfer" pdf

Ngày tải lên : 10/08/2014, 22:21

... corresponding to each antibody Cytotoxicity assay TCR gene-transferred CD3 +T cells (effector cells) or TCR CD3 +T cells that were not gene-transduced were incubated with Toledo cells (DLBCL cell line, ATCC, ... following the cocultivation of effector cells (TCR gene-transduced CD3 +T cells or CD3 +T cells transduced with empty plasmid) with target cells (Toledo, Molt-4, and Raji cells) The specific cytotoxicity ... which aim directly at autologous lymphoma cells It is a pity that we were unable to obtain patients’ autologous lymphoma cells as target cells With respect to the most commonly distributed human MHC...

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Báo cáo y học: " Cross-talk between cd1d-restricted nkt cells and gδ cells in t regulatory cell response" ppt

Ngày tải lên : 11/08/2014, 21:21

... investigating oral tolerance to nickel demonstrated that antigen presenting cells interact with type NKT cells through CD1d causing the NKT cells to secrete IL-4 and IL-10 and activate Treg cells ... gδ cells meaning that the innate effector with the stronger binding avidity would dominate The relevant point is that the balance between NKT and gδ activation can be the deciding factor between ... on the dendritic cells making them less effective in antigen presentation Third, Treg cells can either kill or inhibit T cell differentiation TGFb produced by Treg cells will activate NOTCH and...

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Báo cáo y học: "Analysis of the 5''''UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages" potx

Ngày tải lên : 12/08/2014, 04:20

... We are reporting the isolation and replication of HCV from patients infected by type strains of HCV These new isolates can be cultured in both B and T cells By contrast to type strains of HCV, ... Results presented here show that we were able to isolate and culture HCV from patients infected with HCV-3 to a limited extent However, the HCV-3 produced by macrophages, B -cells, and T- cells were ... 314 T1 and 314 T2 were the same, showing that consecutive transfers of HCV into the same cell type not affect the sequence The 314 T1 and T2 sequences were almost identical to genotype H77, therefore...

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Báo cáo y học: "Evidence that Gag facilitates HIV-1 envelope association both in GPI-enriched plasma membrane and detergent resistant membranes and facilitates envelope incorporation onto virions in primary CD4+ T cells" doc

Ngày tải lên : 12/08/2014, 04:21

... respectively As shown in Figure 2, L30E mutation in Gag restricted envelope association with DRM fractions of CD4+ T cells in contrast to the wild type Our results were further substantiated by the ... from CD4+ T cells infected with wild type and L30E expressing equal amounts of p24 and gp41 were incubated with anti-gp41 monoclonal antibodies to immuno-precipitate Env Briefly, CD4 + T cells were ... also known to be associated with DRM [38] Our data showed that abrogation of Gag-Env interaction down modulated envelope transport into CD59 positive compartment in primary CD4+ T cells and also...

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Báo cáo y học: "NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigenspecific CD4+ T cell polarization" pot

Ngày tải lên : 12/08/2014, 11:22

... polarizing cytokines, it is important to remember that these co-cultures did not include epithelial cells It is likely that epithelial cells also influence the outcome of DC and CD4+ T cell interactions, ... CD1 1b are typically expressed by myeloid DCs, the subset of DCs believed to be exceptionally well-suited for antigen presentation and activation of naïve CD4 + T cells to become T effector cells ... expression, with future analyses focusing on the CD11c+CD1 1b+ population (bold gate, A) The total number of CD11c+CD1 1b+ cells within the lung was enumerated (B) and the maturation status of these cells...

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Báo cáo y học: "Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis" docx

Ngày tải lên : 12/08/2014, 14:20

... cant difference either between patients and controls or between patient subgroups (data not shown) TIM-1 was not detectable on T cells from either patients or controls Discussion In this study, ... Institutet, that were distributed to Johan Grunewald, Anders Eklund and Jan Wahlström and were important for the study design, data collection, analysis and interpretation of data Statement on ... BALF CD4+ T cells It has been reported that the interaction between TIM-3 and its ligand, galectin-9, could downregulate Th1 responses [16] Because of the decreased TIM-3 expression in BALF CD4+ ...

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Báo cáo y học: "Defective response of CD4+ T cells to retinoic acid and TGFb in systemic lupus erythematosus" ppt

Ngày tải lên : 12/08/2014, 17:22

... demonstrated that the induction of Tregs by TGFb and RA was defective in SLE patients and that PBX1-d expression in CD4 + T cells impaired the regulation of FOXP3 and CD25 by TGFb and RA on these cells ... between patients with active and inactive disease, or between patients that were treated or non-treated with steroids (data not shown) Differential distribution of expanded CD4+ T cell subsets ... Interestingly, the CD25 Tregs were also expanded by the combination of RA and TGBb, and these cells responded less to TGBb and to the combination of TGBb and RA in SLE patients than HCs Finally, the CD25...

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Báo cáo y học: "Immunologists getting nervous: neuropeptides, dendritic cells and T cell activation" ppsx

Ngày tải lên : 12/08/2014, 18:20

... [39] The precise contribution of somatostatin to the immunostimulatory function of DCs remains to be determined, although certain DC subsets contain immunoreactive somatostatin VIP and the structurally ... neuropeptides on T cell activation should therefore take into account not only the direct effects of these mediators on T cells but also their indirect effects through the modulation of DC function ... its receptor led to the hypothesis that SP not only acts as a mediator of the crosstalk between the nervous and immune systems but is also biologically involved in the direct interaction between...

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Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx

Ngày tải lên : 12/08/2014, 23:20

... Considering the up-regulatory effect of NF- B with regard to HIV-1 transcription and the potent induction of this transactivator by TLR2 stimulation, we thought that the TLR2-mediated augmentation in ... efficient than TLR5, and stimulations as evidenced by the higher production of IL-6, TNF-α and RANTES Knowing that TLR4 stimulation can activate pathways resulting in both NF- B activation and secretion ... Figure triggering modulates HIV-1 transfer between IM-MDDCs and CD4+ T cells TLR2 and TLR2 and triggering modulates HIV-1 transfer between IM-MDDCs and CD4+ T cells A) IM-MDDCs were either left untreated...

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