cd4 memory t cells to inflammatory and homeostatic chemokines

... after total knee/hip arthroplasty Patients were informed about the purpose of the study and gave written consent Approval for the study was granted by our ethics committee Patient characteristics ... indicating that both CD4+ and CD4- NKT cells in the peripheral blood of RA patients are biased toward a Th1-like cytokine profile To exclude the possibility that the observations in NKT cell ... order to exert their regulatory function NKT cells express a chemokine receptor profile similar to Th1-type inflammatory homing cells, which suggests that these cells perform their function mainly...

Ngày tải lên: 09/08/2014, 06:22

... characteristics Patient characteristics are shown in Table for the 22 patients treated with IFN-a 2b, the 22 patients not treated with IFN-a 2b, and the 20 healthy subjects Of the 22 treated patients, ... with IFN-a 2b treatment may indeed contribute to the antitumor response A better understanding of the mechanism of action of IFN-a 2b may facilitate the development of treatment strategies to ... Flow cytometry: percentage of CD4+ CD25+HFFoxp3+ cells in interferon-a 2b-treated patients with melanoma Flow cytometric gating strategy to identify Treg cells (A) Dot plot of forward scatter (FSC)...

Ngày tải lên: 18/06/2014, 16:20

... cytokines in the absence of a costimulatory pathway CD4+ CD28– T cells contribute to the cell infiltrate and exhibit increased survival after apoptotic stimuli Resistance to apoptosis in CD28– T cells ... compartment in the periphery [10] The association of CD4+ CD28– T cells with disease status has given rise to the hypothesis that these cells directly contribute to disease manifestations Patients ... reports that the role of CD4+ CD28– T cells in RA pathogenesis may be related to their cytotoxic capability, which may contribute to extraarticular manifestations The higher frequency of these cells...

Ngày tải lên: 09/08/2014, 01:22

... AU and IG contributed to the ELISPOT assay analyses GC and AV contributed to interpretation of the data Competing interests The authors declare that they have no competing interests Page of (page ... both patients, an EBV-specific T- cell response to latent peptides was detectable at baseline but was not detectable at week 12 Nevertheless, the response to lytic peptides was persistent in both ... EBV-specific T- cell effector functions leading to the lack of control of the EBV viral load These two patients having been treated with the association of anti-TNF antibody and MTX makes it impossible to...

Ngày tải lên: 09/08/2014, 14:21

... patients different Toll-like receptor ligands Cytokine production after stimulation of blood from sepsis patients usingusing different Toll-like receptor ligands Cytokine production after stimulation ... and participated together with H-DV in the interpretation of all data and revised the manuscript for important intellectual content Acknowledgements The present work is dedicated to Holger Poehlmann ... CD16positive monocytes may be subdivided into CD14bright and CD14dim cells The latter subset has morphological and functional similarities to DCs, including a strong capacity to activate naïve T cells...

Ngày tải lên: 13/08/2014, 18:22

... subjects and characterized TCCs specific for this antigen The data obtained suggest that hnRNP-A2 may constitute an important T cell autoantigen in patients with SLE, indicating a potential role ... and two SLE patients, and attributed a possible pathogenic role to these T cells in SLE [24] However, the presence of autoreactive T cells is not limited to patients, but has repeatedly been observed ... Among the numerous autoantigens described, the nucleosome represents an important target structure of the autoimmune attack, leading to the formation of a wide array of autoAbs directed to single...

Ngày tải lên: 09/08/2014, 08:22

... demonstrate a failure of catecholamines to shift T- cell cytokine responses toward the expected Th2 profile in patients with RA; this failure thus generates a cytokine environment that perpetuates ... software, using twodimensional dot plots Determination of apoptosis The proportion of apoptotic cells was evaluated in isolated Tcells of five patients with RA and five HDs T cells were stimulated ... death on cytokine production of stimulated T cells upon coincubation with catecholamines was tested After stimulation of T cells for 48 hours with anti-CD3-mAb and anti-CD28-mAb together with...

Ngày tải lên: 09/08/2014, 08:22

... at each visit to the clinic, the patients were questioned about compliance with the study diet Also, between visits, the nurse made phone calls to the patients and the patients had the opportunity ... correlated to changes of C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) [13] These results suggest that the abnormal lipid levels are related to inflammation On the other hand, treatment ... patents relating to anti-PC The other authors declare that they have no competing interests Available online http://arthritis-research.com/content/10/2/R34 Authors' contributions A-CE participated...

Ngày tải lên: 09/08/2014, 10:23

... related to the fact that most patients had low inflammatory activity However, when separating the patients into those with low DAS28 and those with DAS28 corresponding to moderate activity it was ... relates to cardiovascular risk factors Materials and methods Patients A total of 80 consecutive outpatients with RA at the Rheumatology Department, Karolinska University Hospital Huddinge, Stockholm, ... the patients with rheumatoid cachexia In the present cohort of patients we did not find that DAS28 or CRP differed between those with cachexia and the rest of the patients, probably related to...

Ngày tải lên: 09/08/2014, 13:22

... data HRG contributed to the revision of the draft of the manuscript GDK contributed substantially to the conception and design of the project and to the revision of the draft of the manuscript ... interpretation of data and contributed substantially to the drafting of the manuscript VFP participated in the acquisition, analysis, and interpretation of data KMJD contributed to the acquisition ... Competing interests The authors declare that they have no competing interests Authors' contributions TET participated in the conception and design of the project and in the analysis and interpretation...

Ngày tải lên: 09/08/2014, 01:22

... exclusion method was used to evaluate the potential of direct cytotoxic effect of inhibitors on cells After incubation for 24 hours, the cells were harvested and the percentage cell viability was ... might have affected the production of IL-17 from T cells To evaluate the effects of inflammatory cytokines released by activated PBMC, we tested the effects of several cytokines and chemokines on ... intense than that in RA PBMC (Fig 7b) To confirm the link between PI3K activity and NF-κB, we performed EMSA to determine the NF-κB binding activity after treatment with both LY294002 and PDTC...

Ngày tải lên: 09/08/2014, 06:22

... juxta-articular bone destruction Competing interests The authors declare that they have no competing interests Authors' contributions The initial draft of the report was written by HM and PM Patients ... now on the market, and it would be unethical not to use and even to withhold infliximab treatment from RA patients with active disease We regarded the optimal situation to be one in which the only ... anti-TNF-α therapy for treatment of persistent active disease, despite treatment with methotrexate Patients were enrolled, starting from when infliximab entered the market, from January 2001 to...

Ngày tải lên: 09/08/2014, 10:20

... the new exercises, we presented to the experts the entire hand or foot that included the joint-of-interest from the single joint exercise This allowed us to test whether the presentation of the ... P, Feldmann M, et al.: Infliximab and methotrexate in the treatment of rheumatoid arthritis Anti-Tumor Necrosis Factor Trial in Rheumatoid Arthritis with Concomitant Therapy Study Group N Engl ... Martin Mola E, Pavelka K, Sany J, Settas J, et al.: Therapeutic effect of the combination of etanercept and methotrexate compared with each treatment alone in patients with rheumatoid arthritis:...

Ngày tải lên: 09/08/2014, 10:20

... study in patients with RA – the first of its kind in humans The study's primary objective was to investigate safety and tolerability; therefore, it was not powered to investigate efficacy or to ... conducted the clinical study as investigators and contributed to the writing of the manuscript LJ, WvdB and HG performed the preclinical studies and contributed to the writing of the manuscript TW ... blocker rather than anakinra as a first biologic agent to treat RA patients, concordant with a recent consensus statement, and national and international registries [3] Treatment with recombinant IL-1Ra...

Ngày tải lên: 09/08/2014, 10:23

... data HRG contributed to the revision of the draft of the manuscript GDK contributed substantially to the conception and design of the project and to the revision of the draft of the manuscript ... interpretation of data and contributed substantially to the drafting of the manuscript VFP participated in the acquisition, analysis, and interpretation of data KMJD contributed to the acquisition ... Competing interests The authors declare that they have no competing interests Authors' contributions TET participated in the conception and design of the project and in the analysis and interpretation...

Ngày tải lên: 09/08/2014, 13:22

... years followup Of the 163 patients in this study, 128 had X-rays at all four time points, 29 at three time points and six patients at two time points The patients with and without hand X-rays had ... antiCCP and ESR as important predictors of bone involvement in RA The bone involvement in RA has been shown to start in the inflamed synovium that express receptor activator of nuclear factor-κB ... statistical analyses and prepared the manuscript MH contributed in the statistical analyses and substantially contributed to the manuscript SØ organised the clinical data collection GH organised the...

Ngày tải lên: 09/08/2014, 14:22

... unrestricted random method of allocation by the research assistant Both the primary investigator and consultant radiologist were blinded to the image selection procedure The two investigators ... Thirty two patients with RA were recruited One patient withdrew due to time issues during the visit Thirty one patients completed the study There were 24 female and male patients, 12 seronegative and ... helped to draft the manuscript SS participated in study design and coordination JB participated in the design of the study and helped to draft the manuscript CE participated in the design of the study,...

Ngày tải lên: 10/08/2014, 21:23

... sought to identify respiratory symptoms and additional patient characteristics that, alone or in combination, discriminated patients with PFT abnormalities Page of 11 Materials and methods Participants ... In contrast to previous studies, ours is the first study to exclude patients with clinically apparent CVD, thus increasing the likelihood that the reported respiratory symptoms truly reflected ... on symptoms, PFT and HRCT results, but did not test predictor variables against a uniform outcome [12] The study by Gochuico and colleagues enrolled 64 RA patients without respiratory symptoms...

Ngày tải lên: 12/08/2014, 14:21

... consecutive rheumatoid arthritis (RA) patients were recruited from the rheumatology outpatient clinics of the Dudley Group of Hospitals NHS Trust, UK All patients met the retrospective application ... according to the Declaration of Helsinki Protocol Patients reported to a temperature-controlled vascular laboratory (22°C) after a 12-hour overnight fast For ethical reasons, patients were not asked to ... the vasodilatory response between the two assessments In the current work, participants were not asked to withhold their antirheumatic drug treatment or vasoactive medications prior to the vascular...

Ngày tải lên: 12/08/2014, 17:21

... impact on the quality of life of the patient It also associates with very high costs to the health system Hence, it is important to identify strategies that may improve overall management and ... admitted) stayed as inpatients at the hospital after they were admitted The length of hospitalisation for these 32 patients was: a) between one to five days for eight patients (25% of the 32 patients), ... important parameters that may affect cost of treatment and quality of life Therefore, substantial effort has being devoted to devise effective and safe interventions to reduce preventable hospital admissions...

Ngày tải lên: 12/08/2014, 17:22