cd34 mouse hematopoietic stem cells

... statistical significance. Results Stem- Kine mobilizes CD34 and CD133 Cells Quantification of peripheral blood cells expressing the hematopoietic stem cell markers CD133 and CD34 was performed at day ... human stem cells. Stem Cells Dev 2006, 15(1):118-23. 77. Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem cells: protection ... These data suggest Stem- Kine supplementation increases circula- tion of cells capable of giving rise to hematopoietic- lineage cells in vitro. Stem- Kine augments circulation of cells with EPC phenotype Agents...

Ngày tải lên: 18/06/2014, 16:20

... relevant signaling pathways in hematopoietic stem cells are still poorly understood. Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for ... erythroid cells. The cultivation of erythroid cells derived from HSCs /CD34 + cells was performed according to a procedure previously described [13] with some modifications. HSCs /CD34 + cells were ... cellular pathway analysis of identified proteins in this study. Abbreviations HSCs /CD34 + : CD34 + hematopoietic stem cells; HbE/β-thalassemia: hemoglobin E beta thalassemia; IMAC: immobilized metal...

Ngày tải lên: 18/06/2014, 19:20

... relevant signaling pathways in hematopoietic stem cells are still poorly understood. Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for ... erythroid cells. The cultivation of erythroid cells derived from HSCs /CD34 + cells was performed according to a procedure previously described [13] with some modifications. HSCs /CD34 + cells were ... cellular pathway analysis of identified proteins in this study. Abbreviations HSCs /CD34 + : CD34 + hematopoietic stem cells; HbE/β-thalassemia: hemoglobin E beta thalassemia; IMAC: immobilized metal...

Ngày tải lên: 20/06/2014, 04:20

... Chapter 68. Hematopoietic Stem Cells Hematopoietic Stem Cells: Introduction All of the cell types in the peripheral blood and some cells in every tissue of the body are derived from hematopoietic ... the clinical use of hematopoietic stem cells, tens of thousands of lives are saved each year (Chap. 108). Stem cells produce tens of billions of blood cells daily from a stem cell pool that ... much of what we know about stem cells is imprecise and based on inferences from genetically manipulated animals. Cardinal Functions of Hematopoietic Stem Cells All stem cell types have two cardinal...

Ngày tải lên: 07/07/2014, 01:20

... Chapter 068. Hematopoietic Stem Cells (Part 2) Developmental Biology of Hematopoietic Stem Cells During development, blood cells are produced at different sites. ... the so-called stem cell niche. Hematopoietic Stem Cell Microenvironment The concept of a specialized microenvironment, or stem cell niche, was first proposed to explain why cells derived from ... the receptor can all result in the release of stem cells into the circulation. This process is an increasingly important aspect of recovering stem cells for therapeutic use as it has permitted...

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Ngày tải lên: 24/08/2014, 09:44

... Flk-1+CD31 -CD34- mesenchymal stem cells: feasibility and safety from monkey to human. Stem Cells Dev,2006,15(3):349-357 41. Devine SM, Bartholomew AM, Mahmud N, et al. Mesenchymal stem cells are ... injured tissues play important roles. (2) Stem cells circulate among tissues, and stem cells migrate to the injured tissues once injury occurs. Stem cell homing is a complicated process in ... of stem cells, it is very important to observe the survival and cura- tive effects of transplanted stem cells. Traditional immunohistochemical method can easily identify the transplanted cells...

Ngày tải lên: 25/10/2012, 11:18

... break-down and power failure. Key words: human embryonic, stem cells, low temperature 1. Introduction In vitro culture of human embryonic stem cells (hESC) often involves temporary exposure to reduced ... efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells. Stem Cells. 2004;22(5):779-89. 10. Speicher MR, Ballard SG, Ward DC. Karyotyping human chromosomes ... some cells detached after exposure to low temperature. Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown). All of the detached cells...

Ngày tải lên: 31/10/2012, 16:57

... mesenchymal stem cells. Science 284, 143–147. 2 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications. Curr Stem Cell ... (2007) Effect of hypoxia on gene expression of bone marrow- derived mesenchymal stem cells and mononuclear cells. Stem Cells 25, 1166–1177. 7 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) ... mesenchymal stem cells (MSCs) are easily obtainable and expandable, multipotent progeni- tor cells [1] that have emerged as attractive candidates for cellular therapies for heart and other organ-system disorders...

Ngày tải lên: 18/02/2014, 04:20

... triggering. There is another example of stemness decisions by Nodal versus BMP signaling emerging from human embryonic stem cell research. Testing human ES -cells BMP signaling in stem cells T. U. Wagner 2972 ... embryonic stem cells. Stem Cells 22, 770–778. 22 Ying QL, Nichols J, Chambers I & Smith A (2003) BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal ... signals in stem cells Bone morphogenetic protein (BMP) signals have tre- mendous effects on all kinds of cells. Most striking and defining, however, are the reactions that stem and progenitor cells...

Ngày tải lên: 19/02/2014, 00:20

... microscope. Construction of mouse Hsp105a expression plasmid and isolation of Hsp105a-over-expressing cells Plasmid pcDNA105a wasusedtoexpressmouseHsp105a in F9 cells. To construct this plasmid, the mouse Hsp105a cDNA ... lgặmL )1 ethidium bromide. Morphological examination of apoptotic cells Cells were plated onto coverslips at a density of 1 Ã 10 5 cells cm )2 and grown at 37 °C for 24 h. After treatment, cells were washed with NaCl/P i , ... cells showed an enlarged and flattened morphology as seen in F9 or V1 cells, which is characteristic of primitive endoderm-like cells (Fig. 1C, e–h). In addition, the Hsp105a over-expressing cells were...

Ngày tải lên: 21/02/2014, 03:20